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The map label for this gene is thiO/thiG [H]

Identifier: 220915278

GI number: 220915278

Start: 175634

End: 176764

Strand: Reverse

Name: thiO/thiG [H]

Synonym: A2cp1_0157

Alternate gene names: 220915278

Gene position: 176764-175634 (Counterclockwise)

Preceding gene: 220915279

Following gene: 220915277

Centisome position: 3.51

GC content: 78.25

Gene sequence:

>1131_bases
ATGAAGACGGACGTGGTGGTGGTGGGCGCCGGGCTGCAGGGCGCCTCGGTGGCGCTGCGCCTCGCGCAGGCCGGCAAGCA
GGTGGTGGTGCTGGAGCGGGCGGTGCCGGGCGCCGAGGCGTCCTACGCGGCCGGCGGCATCCTCTCGCCCGGCGTGGAGG
CGCTCGAGCCGGGCCCGTTCTACGCGCTCTGCGCCGCCTCGCTGGCGCGCTACCCGGCGTTCGCGCGCGAGGTCGAGGCC
GCGAGCGGCATGTGGGTCGGCCTCCGCGGCGGCGGGACGCTCGAGATCGCGGTGGACGATCCGGAGGCCCGGCTCCTCGC
CGGGCGCGCCGAGAAGCTCCACAAGGCCGGGCTGGCGGTGGAGGTGCTGGACGACGCCGAGGTGCGCCGGCTCGAGCCGG
GCGTGTCGCCGGAGACGCGCGGCGCGCTGTACTTCGCCGACGAGGCCTCGGTGGACCCGCGGCTGCTGGGCCGGGCCGTG
TACGTGGCGGCGGCGCGCGCCGGGGCCCGCTTCGTCACCGGCAAGGTGGAGCGGATCGTCCACGCGGGCGGGCGGGCGGT
GGGCGTGGACCACGACGCCGGGCGCATCGAGGCGGACGCGGTGGTGCTCGCCGCGGGGAGCTGGAGCATGCAGGTGGAGG
GGAACGGGCTGCCGCCCGGCGCGGTGCGCCCGGTGCGCGGGCAGATCGCGCTCCTCGACACCCGGCCGCCGCTCCTCTCG
CGCGTGGTGTTCTCGGCCAACGGCTACGTGGTGCCGCGCGCCGACGGGCGGATCCTGTGCGGCTCCACCATGGAGGAGGT
CGGGCACGAGAAGGCGGTGACCGCGGGCGGGCTGCGGCGCGTCCTCGACATCGCGCTCGAGATCGCGCCCGCGCTGGCGG
CCGCGCCGGTGGTCGAGACCTGGTCCAACTTCCGCCCCGCCAGCCCGGACGGCTCGCCCATCCTCGGCGCCGGGACCGTG
CCCGGCCTGTACTACGCCACCGGCCACACGCGGAACGGCATCCTGCTCGCGCCGGTCACCGCCGACGCGGTGGCGGCGGC
CGTGCTCGGGACGCCGCCGCCGGTGGACCTCGCGCCGTTCTCGCCGGCGCGCCTCGGGGCGGGCCGGCCGCAGGCGGCGG
GCCGCAGGTAG

Upstream 100 bases:

>100_bases
CGGCTGAGCGCCCGCCGCCCGTCTGCGCGCCCGGCCCGCCGTCCCGGCGGGGGCACGCGAGCCTTTCCGGCGCGCCGGGG
CGGGCGTAGGCTCGCGCACC

Downstream 100 bases:

>100_bases
GCGCGGGCTCCCCCGGCTTGCCCGCGGCCGCGCCGGGCCGCAGGTTTTCACCGGCCCATGCGCGGCGCGCCGGACATCCG
GGAGGTGCAGGACCACCTCT

Product: glycine oxidase ThiO

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: Probable FAD-dependent glycine oxidase; Thiazole synthase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKTDVVVVGAGLQGASVALRLAQAGKQVVVLERAVPGAEASYAAGGILSPGVEALEPGPFYALCAASLARYPAFAREVEA
ASGMWVGLRGGGTLEIAVDDPEARLLAGRAEKLHKAGLAVEVLDDAEVRRLEPGVSPETRGALYFADEASVDPRLLGRAV
YVAAARAGARFVTGKVERIVHAGGRAVGVDHDAGRIEADAVVLAAGSWSMQVEGNGLPPGAVRPVRGQIALLDTRPPLLS
RVVFSANGYVVPRADGRILCGSTMEEVGHEKAVTAGGLRRVLDIALEIAPALAAAPVVETWSNFRPASPDGSPILGAGTV
PGLYYATGHTRNGILLAPVTADAVAAAVLGTPPPVDLAPFSPARLGAGRPQAAGRR

Sequences:

>Translated_376_residues
MKTDVVVVGAGLQGASVALRLAQAGKQVVVLERAVPGAEASYAAGGILSPGVEALEPGPFYALCAASLARYPAFAREVEA
ASGMWVGLRGGGTLEIAVDDPEARLLAGRAEKLHKAGLAVEVLDDAEVRRLEPGVSPETRGALYFADEASVDPRLLGRAV
YVAAARAGARFVTGKVERIVHAGGRAVGVDHDAGRIEADAVVLAAGSWSMQVEGNGLPPGAVRPVRGQIALLDTRPPLLS
RVVFSANGYVVPRADGRILCGSTMEEVGHEKAVTAGGLRRVLDIALEIAPALAAAPVVETWSNFRPASPDGSPILGAGTV
PGLYYATGHTRNGILLAPVTADAVAAAVLGTPPPVDLAPFSPARLGAGRPQAAGRR
>Mature_376_residues
MKTDVVVVGAGLQGASVALRLAQAGKQVVVLERAVPGAEASYAAGGILSPGVEALEPGPFYALCAASLARYPAFAREVEA
ASGMWVGLRGGGTLEIAVDDPEARLLAGRAEKLHKAGLAVEVLDDAEVRRLEPGVSPETRGALYFADEASVDPRLLGRAV
YVAAARAGARFVTGKVERIVHAGGRAVGVDHDAGRIEADAVVLAAGSWSMQVEGNGLPPGAVRPVRGQIALLDTRPPLLS
RVVFSANGYVVPRADGRILCGSTMEEVGHEKAVTAGGLRRVLDIALEIAPALAAAPVVETWSNFRPASPDGSPILGAGTV
PGLYYATGHTRNGILLAPVTADAVAAAVLGTPPPVDLAPFSPARLGAGRPQAAGRR

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=420, Percent_Identity=24.7619047619048, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006076
- InterPro:   IPR012727
- InterPro:   IPR008867 [H]

Pfam domain/function: PF01266 DAO; PF05690 ThiG [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 38412; Mature: 38412

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTDVVVVGAGLQGASVALRLAQAGKQVVVLERAVPGAEASYAAGGILSPGVEALEPGPF
CCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCH
YALCAASLARYPAFAREVEAASGMWVGLRGGGTLEIAVDDPEARLLAGRAEKLHKAGLAV
HHHHHHHHHHCCHHHHHHHHCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEE
EVLDDAEVRRLEPGVSPETRGALYFADEASVDPRLLGRAVYVAAARAGARFVTGKVERIV
EEECCCHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHEEEHHHCCCEEHHHHHHHHH
HAGGRAVGVDHDAGRIEADAVVLAAGSWSMQVEGNGLPPGAVRPVRGQIALLDTRPPLLS
HCCCCEEECCCCCCCEEECEEEEEECCEEEEEECCCCCCCCCCCCCCEEEEEECCCHHHH
RVVFSANGYVVPRADGRILCGSTMEEVGHEKAVTAGGLRRVLDIALEIAPALAAAPVVET
HHEECCCCEEEECCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSNFRPASPDGSPILGAGTVPGLYYATGHTRNGILLAPVTADAVAAAVLGTPPPVDLAPF
HHCCCCCCCCCCEEEECCCCCCEEEECCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCC
SPARLGAGRPQAAGRR
CCHHCCCCCCCCCCCC
>Mature Secondary Structure
MKTDVVVVGAGLQGASVALRLAQAGKQVVVLERAVPGAEASYAAGGILSPGVEALEPGPF
CCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCH
YALCAASLARYPAFAREVEAASGMWVGLRGGGTLEIAVDDPEARLLAGRAEKLHKAGLAV
HHHHHHHHHHCCHHHHHHHHCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEE
EVLDDAEVRRLEPGVSPETRGALYFADEASVDPRLLGRAVYVAAARAGARFVTGKVERIV
EEECCCHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHEEEHHHCCCEEHHHHHHHHH
HAGGRAVGVDHDAGRIEADAVVLAAGSWSMQVEGNGLPPGAVRPVRGQIALLDTRPPLLS
HCCCCEEECCCCCCCEEECEEEEEECCEEEEEECCCCCCCCCCCCCCEEEEEECCCHHHH
RVVFSANGYVVPRADGRILCGSTMEEVGHEKAVTAGGLRRVLDIALEIAPALAAAPVVET
HHEECCCCEEEECCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSNFRPASPDGSPILGAGTVPGLYYATGHTRNGILLAPVTADAVAAAVLGTPPPVDLAPF
HHCCCCCCCCCCEEEECCCCCCEEEECCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCC
SPARLGAGRPQAAGRR
CCHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 14 {D-glutamine}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]