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The map label for this gene is ispE

Identifier: 220915262

GI number: 220915262

Start: 158138

End: 159079

Strand: Reverse

Name: ispE

Synonym: A2cp1_0141

Alternate gene names: 220915262

Gene position: 159079-158138 (Counterclockwise)

Preceding gene: 220915276

Following gene: 220915261

Centisome position: 3.16

GC content: 76.33

Gene sequence:

>942_bases
GTGCGCCTCGTCACGCTCGCCCCGGCCAAGGTGAACCTCGTGCTCCGCGTGGGCCCGGTCCGCGCGGACGGCTACCACGA
CCTCCGCACGCTGATGGTCCCGCTCGACCTCGGCGATCGCGTGGACGTCCGCGTGTCGCCGCGCCGCGGGCCGGTCCGCT
GCACGGTGCCGGGCCGGCCGGAGCTGGACGGCCCGGAGAACCTGGCGGCGCGCGCCGCCGAGGCGTTCCGCCGGCGCTTC
GGCGTCGATCGCGCGGTGTCGATCCGGATCGAGAAGCGTACGCCGGTGACCGCCGGGCTGGGCGGGGGCTCCTCCGACGC
GGCGGCGGTGCTCCGCTGCCTCGCCCGCGCGCTGCGGGTGCGCGACGGCGCGGCGCTCGCCGCGCTCGCGCTCGAGATCG
GCTCGGACGTTCCGTTCTTCCTCGGCCCCGGGCCGGCGTGGGCGGCGGGGCGCGGCGAGCGGCTCTCCCGCGCCGAGGTG
CCGCCGCTCGACCTCGTGCTCGTGTATCCGGCCGATCCTTCGCTCGCGATCCGGGCGGGCGACGCCTACCGCTGGCTGGA
CGAGGCGCGCGCCAGCGGGTCGCAGGCCCCGCGGCGTCTGGGCCGTCCGGGGCGGTGGCGTCCGACCCTGCTGGGGAACG
ATCTCCAGGCGCCCTGCGTGGCGCGAAAGCCCGCGCTCCAGGCGCTGCTTGGGCTGCTTGTGGGGGCGGGGGCGACGGCC
GCTATAATGTCCGGCAGCGGGCCGACCGTCTTCGGAATCTTCCCGGGCCGAGGCGCGGCGCGCGGGGCGGCGTTAGCGAT
CCAGGGCAGGGCGAAGGGGGGCGCGGCTGGCGTGCAGGTCCTCCTTGCGCGGACGGTGCGACGGCATCCGAGGGTATCGC
CATGGAGATCACCGAGGTCCGCGTCTTCCCGGTCAACGAGGAGAAGCTCAAGGCCTACGTGA

Upstream 100 bases:

>100_bases
GCCCTCGGGGAGAAGGAGTAAGCGCGCGCACCTCCGTGACACGTGCATCCGCGCGCAGGCCCGGCGCACCGGCGCGCGCG
ACCGTGGTAGAGGCCTCTCC

Downstream 100 bases:

>100_bases
CGATCACGCTGGATCACTGCTTCGTGATCCGCGACCTGAAGGTGATCCACGGCAACACCGGCCTGTTCATCGCGATGCCG
GCCAAGCGCCGCAAGGACGG

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MRLVTLAPAKVNLVLRVGPVRADGYHDLRTLMVPLDLGDRVDVRVSPRRGPVRCTVPGRPELDGPENLAARAAEAFRRRF
GVDRAVSIRIEKRTPVTAGLGGGSSDAAAVLRCLARALRVRDGAALAALALEIGSDVPFFLGPGPAWAAGRGERLSRAEV
PPLDLVLVYPADPSLAIRAGDAYRWLDEARASGSQAPRRLGRPGRWRPTLLGNDLQAPCVARKPALQALLGLLVGAGATA
AIMSGSGPTVFGIFPGRGAARGAALAIQGRAKGGAAGVQVLLARTVRRHPRVSPWRSPRSASSRSTRRSSRPT

Sequences:

>Translated_313_residues
MRLVTLAPAKVNLVLRVGPVRADGYHDLRTLMVPLDLGDRVDVRVSPRRGPVRCTVPGRPELDGPENLAARAAEAFRRRF
GVDRAVSIRIEKRTPVTAGLGGGSSDAAAVLRCLARALRVRDGAALAALALEIGSDVPFFLGPGPAWAAGRGERLSRAEV
PPLDLVLVYPADPSLAIRAGDAYRWLDEARASGSQAPRRLGRPGRWRPTLLGNDLQAPCVARKPALQALLGLLVGAGATA
AIMSGSGPTVFGIFPGRGAARGAALAIQGRAKGGAAGVQVLLARTVRRHPRVSPWRSPRSASSRSTRRSSRPT
>Mature_313_residues
MRLVTLAPAKVNLVLRVGPVRADGYHDLRTLMVPLDLGDRVDVRVSPRRGPVRCTVPGRPELDGPENLAARAAEAFRRRF
GVDRAVSIRIEKRTPVTAGLGGGSSDAAAVLRCLARALRVRDGAALAALALEIGSDVPFFLGPGPAWAAGRGERLSRAEV
PPLDLVLVYPADPSLAIRAGDAYRWLDEARASGSQAPRRLGRPGRWRPTLLGNDLQAPCVARKPALQALLGLLVGAGATA
AIMSGSGPTVFGIFPGRGAARGAALAIQGRAKGGAAGVQVLLARTVRRHPRVSPWRSPRSASSRSTRRSSRPT

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=272, Percent_Identity=32.7205882352941, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_ANAD2 (B8J808)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002490566.1
- ProteinModelPortal:   B8J808
- SMR:   B8J808
- GeneID:   7299783
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_0141
- HOGENOM:   HBG734593
- ProtClustDB:   CLSK944838
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 32964; Mature: 32964

Theoretical pI: Translated: 12.23; Mature: 12.23

Prosite motif: NA

Important sites: ACT_SITE 10-10 ACT_SITE 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLVTLAPAKVNLVLRVGPVRADGYHDLRTLMVPLDLGDRVDVRVSPRRGPVRCTVPGRP
CEEEEECCCEEEEEEEECCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCCEEEECCCCC
ELDGPENLAARAAEAFRRRFGVDRAVSIRIEKRTPVTAGLGGGSSDAAAVLRCLARALRV
CCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHC
RDGAALAALALEIGSDVPFFLGPGPAWAAGRGERLSRAEVPPLDLVLVYPADPSLAIRAG
CCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEC
DAYRWLDEARASGSQAPRRLGRPGRWRPTLLGNDLQAPCVARKPALQALLGLLVGAGATA
CHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCE
AIMSGSGPTVFGIFPGRGAARGAALAIQGRAKGGAAGVQVLLARTVRRHPRVSPWRSPRS
EEECCCCCEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
ASSRSTRRSSRPT
CCHHHHHHCCCCC
>Mature Secondary Structure
MRLVTLAPAKVNLVLRVGPVRADGYHDLRTLMVPLDLGDRVDVRVSPRRGPVRCTVPGRP
CEEEEECCCEEEEEEEECCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCCEEEECCCCC
ELDGPENLAARAAEAFRRRFGVDRAVSIRIEKRTPVTAGLGGGSSDAAAVLRCLARALRV
CCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHC
RDGAALAALALEIGSDVPFFLGPGPAWAAGRGERLSRAEVPPLDLVLVYPADPSLAIRAG
CCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEC
DAYRWLDEARASGSQAPRRLGRPGRWRPTLLGNDLQAPCVARKPALQALLGLLVGAGATA
CHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCE
AIMSGSGPTVFGIFPGRGAARGAALAIQGRAKGGAAGVQVLLARTVRRHPRVSPWRSPRS
EEECCCCCEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
ASSRSTRRSSRPT
CCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA