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The map label for this gene is ansA [H]

Identifier: 220905599

GI number: 220905599

Start: 2817713

End: 2818771

Strand: Reverse

Name: ansA [H]

Synonym: Ddes_2339

Alternate gene names: 220905599

Gene position: 2818771-2817713 (Counterclockwise)

Preceding gene: 220905600

Following gene: 220905598

Centisome position: 98.1

GC content: 65.53

Gene sequence:

>1059_bases
ATGAGCCGTTCTTTTGATTCCGCTGCTGCTCCGTGCGGCGCGCCGCTGCCCCAGGTGGCCCTGCTGGGGACGGGCGGAAC
CATTGCCGGGGTGGCCGACACCCCGCTGGAACAGATAAGTTATCAGGCGGGTGTGCTTTCTGTGGAGTGCATGCTGGAAA
GCCTGCCGGGTATTGACGGCGTGGCCCGCATCGCCAGCCGCCAGTGCGGTAACCTGCCCAGCGAGGATATGCGCCCCTGT
CATTGGGCCAAGCTCGGGCGCGACTGTGGCGCGGCTCTGGATGACGCATCCATGTGTGGGGTGGTGCTGACCCACGGCAC
AGACACGCTTGAGGAGTCGGCTTTTTACCTGCATCTGACCATGAGCAGCCCCAAACCTGTGGTGCTTACCGGGGCCATGC
GTCCGGCCACATCCCTGAGTGCGGACGGTCCCATCAATATTCGCGATGCCGTGGCCGTGGCGGCCAGCCCCGGGGCGCGC
GGGCGTGGTGCCCTTATTGTGATGAACGGGCGAATTCTTTCGGCGCGCACGGCGGTGAAGGCCGGAACACTTGATGTGGA
GGCCTTTTGCGCTCTGGAATCAGGGCTGCTCGGCCGGGTCATCAACGGCCGCCCTGTTTTTTACCACAAGGGCGAGGATG
AACCGCCTCTGGCAGGGACCTATGCGGCCCATGCGGGGCAGGAGCGCTTGCCCCGCGTGGACGTGCTTTATGCCTGTGCC
GGAATGCCTCTGGACGCGGTGCTCTTTTCTATGGAGCGTTCGGCCGGACTTGTGGTGGCTGGTATGGGCAATGGCTCGAT
GCCTTCAGATGTGCGCAAGGCTCTTGCCGGGGCTGTTGCTGCGGGCAGGCCCGTGGTACGGGCCAGCCGCACGGGTGGCG
GCCCTGTGACGGATCTGGACGGATACGCACCCTTTATAGCCTCGGGCATGCTTTCCGCGCCCAAGGCGCGGGTGCTGCTC
ATGCTGGCGCTGGCCGAAGCCGCCCGGCAGGGGGGGCAGAGTCCGCAGGCGCTGACTGCAGGCGTGCGTCGGGCTTTTGA
ATGCTGCCACGAGGCATGA

Upstream 100 bases:

>100_bases
TGCGGCGGGCAGCTTCCGGCCGGATGCGGGTCAACCTGAAACTGCAAGTCCGCGCGGCTGCTCCCCGGCAACAGGAGGGG
ACTGCGCGGCGGGAGCCGTC

Downstream 100 bases:

>100_bases
CGGAACGAAACATGATGTTTTTTCCTGACTGCGCCGGGCATGTTGCGTCGCCGGACCATTTGACTTGCCTGAGAGATTGA
AAAGACGCACAATCGCCCTG

Product: Asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MSRSFDSAAAPCGAPLPQVALLGTGGTIAGVADTPLEQISYQAGVLSVECMLESLPGIDGVARIASRQCGNLPSEDMRPC
HWAKLGRDCGAALDDASMCGVVLTHGTDTLEESAFYLHLTMSSPKPVVLTGAMRPATSLSADGPINIRDAVAVAASPGAR
GRGALIVMNGRILSARTAVKAGTLDVEAFCALESGLLGRVINGRPVFYHKGEDEPPLAGTYAAHAGQERLPRVDVLYACA
GMPLDAVLFSMERSAGLVVAGMGNGSMPSDVRKALAGAVAAGRPVVRASRTGGGPVTDLDGYAPFIASGMLSAPKARVLL
MLALAEAARQGGQSPQALTAGVRRAFECCHEA

Sequences:

>Translated_352_residues
MSRSFDSAAAPCGAPLPQVALLGTGGTIAGVADTPLEQISYQAGVLSVECMLESLPGIDGVARIASRQCGNLPSEDMRPC
HWAKLGRDCGAALDDASMCGVVLTHGTDTLEESAFYLHLTMSSPKPVVLTGAMRPATSLSADGPINIRDAVAVAASPGAR
GRGALIVMNGRILSARTAVKAGTLDVEAFCALESGLLGRVINGRPVFYHKGEDEPPLAGTYAAHAGQERLPRVDVLYACA
GMPLDAVLFSMERSAGLVVAGMGNGSMPSDVRKALAGAVAAGRPVVRASRTGGGPVTDLDGYAPFIASGMLSAPKARVLL
MLALAEAARQGGQSPQALTAGVRRAFECCHEA
>Mature_351_residues
SRSFDSAAAPCGAPLPQVALLGTGGTIAGVADTPLEQISYQAGVLSVECMLESLPGIDGVARIASRQCGNLPSEDMRPCH
WAKLGRDCGAALDDASMCGVVLTHGTDTLEESAFYLHLTMSSPKPVVLTGAMRPATSLSADGPINIRDAVAVAASPGARG
RGALIVMNGRILSARTAVKAGTLDVEAFCALESGLLGRVINGRPVFYHKGEDEPPLAGTYAAHAGQERLPRVDVLYACAG
MPLDAVLFSMERSAGLVVAGMGNGSMPSDVRKALAGAVAAGRPVVRASRTGGGPVTDLDGYAPFIASGMLSAPKARVLLM
LALAEAARQGGQSPQALTAGVRRAFECCHEA

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=330, Percent_Identity=37.2727272727273, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1788065, Length=148, Percent_Identity=31.7567567567568, Blast_Score=68, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6323189, Length=323, Percent_Identity=35.2941176470588, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6323187, Length=323, Percent_Identity=35.2941176470588, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6323186, Length=323, Percent_Identity=35.2941176470588, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6323184, Length=323, Percent_Identity=35.2941176470588, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6320527, Length=323, Percent_Identity=33.1269349845201, Blast_Score=166, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 36008; Mature: 35877

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSFDSAAAPCGAPLPQVALLGTGGTIAGVADTPLEQISYQAGVLSVECMLESLPGIDG
CCCCCCCCCCCCCCCCCCEEEEECCCCEECCCCCCHHHHHHHCCCCHHHHHHHHCCCCHH
VARIASRQCGNLPSEDMRPCHWAKLGRDCGAALDDASMCGVVLTHGTDTLEESAFYLHLT
HHHHHHHHHCCCCCHHCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEEEEEEE
MSSPKPVVLTGAMRPATSLSADGPINIRDAVAVAASPGARGRGALIVMNGRILSARTAVK
ECCCCCEEEECCCCCCCCCCCCCCCCHHHHEEEECCCCCCCCCEEEEECCEEEEHHHHHH
AGTLDVEAFCALESGLLGRVINGRPVFYHKGEDEPPLAGTYAAHAGQERLPRVDVLYACA
CCCCCHHHHHHHHCCCCEEEECCCEEEEECCCCCCCCCCCHHHHCCHHHCCCHHHHHHHC
GMPLDAVLFSMERSAGLVVAGMGNGSMPSDVRKALAGAVAAGRPVVRASRTGGGPVTDLD
CCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
GYAPFIASGMLSAPKARVLLMLALAEAARQGGQSPQALTAGVRRAFECCHEA
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRSFDSAAAPCGAPLPQVALLGTGGTIAGVADTPLEQISYQAGVLSVECMLESLPGIDG
CCCCCCCCCCCCCCCCCEEEEECCCCEECCCCCCHHHHHHHCCCCHHHHHHHHCCCCHH
VARIASRQCGNLPSEDMRPCHWAKLGRDCGAALDDASMCGVVLTHGTDTLEESAFYLHLT
HHHHHHHHHCCCCCHHCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEEEEEEE
MSSPKPVVLTGAMRPATSLSADGPINIRDAVAVAASPGARGRGALIVMNGRILSARTAVK
ECCCCCEEEECCCCCCCCCCCCCCCCHHHHEEEECCCCCCCCCEEEEECCEEEEHHHHHH
AGTLDVEAFCALESGLLGRVINGRPVFYHKGEDEPPLAGTYAAHAGQERLPRVDVLYACA
CCCCCHHHHHHHHCCCCEEEECCCEEEEECCCCCCCCCCCHHHHCCHHHCCCHHHHHHHC
GMPLDAVLFSMERSAGLVVAGMGNGSMPSDVRKALAGAVAAGRPVVRASRTGGGPVTDLD
CCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
GYAPFIASGMLSAPKARVLLMLALAEAARQGGQSPQALTAGVRRAFECCHEA
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]