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The map label for this gene is prfA

Identifier: 220905560

GI number: 220905560

Start: 2766784

End: 2767854

Strand: Reverse

Name: prfA

Synonym: Ddes_2299

Alternate gene names: 220905560

Gene position: 2767854-2766784 (Counterclockwise)

Preceding gene: 220905561

Following gene: 220905556

Centisome position: 96.33

GC content: 58.82

Gene sequence:

>1071_bases
ATGTTCGCCAAATTGGAAGGCCTGGAAAAGAAATATCTGGAGCTTGAAGAGGCCCTTGCCCAGCCTGATGTTTTCAACGA
TCAGGATCAGTACCGCAAGCTGACCAAAGCCCACGCCGACCTGAGCGACGTGGTGGAGCTTTTTCGCCGTCACCGCGCGG
TGAGCGAAGAGCTGGCCGAAAACAGGCTGCTGCTGCACGATGAAGACCCCGAAATCCGTGCCATGGCGCAGGAAGAAGTG
CACCGGGGCGAGGCCGAACTGCCAAATCTTGAGCATGAACTCAAGGTAATGCTGCTGCCCAGCGACCCCCTGGACGAAAA
AAATACCATTCTTGAAATCCGCGCGGGTACGGGCGGCGAAGAGGCTGCCCTGTTTGCAGCGGATCTTTTCCGCATGTATA
CGCGCTATGCCGAGCTTAAAAACTGGAAGGTGGAGATCATGAGTGAATCCCCTTCCGAAAACGGCGGCTATAAGGAAATC
ATCTGCCTTATCGCCGGCGACCGCGTGTACAGCCACCTCAAGTTCGAGGCGGGAACACACCGTGTGCAACGCGTACCCGC
TACCGAGGCGCAGGGCCGTATTCACACCTCGGCCGCTACCGTGGCCGTTATGCCCGAAGCTGAAGAGGTCGACGTTGAAA
TCCGCCCCGAAGACCTGCGCATAGATATTTACCGGGCTTCGGGCGCGGGCGGACAGCACGTCAACAAGACGGAATCGGCC
GTGCGCATCACCCACCTGCCCACCAATACCGTGGTTACCTGTCAGGATGAGCGCTCGCAGCATAAAAACAAGGCCCGCGC
CATGAAGGTGCTTGCCTCGCGTATTCTGGCTGCCGAACGCGAGCGCCAGAGTTCGGAGCTTTCCGCAGACCGCAAGGCAC
AGGTCGGCTCCGGCGACCGTTCGGAGCGTATCCGTACCTATAACTTTCCGCAGGGCCGCTGCACGGACCATCGCATCAAT
CTGACACTGTATTCCCTGGACAGGATTATGGAAGGCGAGTTGGAATCGTTGGTGGAGGCTTTGGGCACAGCCGCCCAGGC
CGAAGCCCTCAAGGCCCAGGCCTCCGAATAG

Upstream 100 bases:

>100_bases
ATTGAAAGTATGACATTTTCGCGAAAGCGTCCTGCCTGTGCGGTTGGGGCGGTTCGCCGCCGCATGAGTTGTTTTTGTCC
ACTGCCAGTCGGAGACGTTT

Downstream 100 bases:

>100_bases
TTCCCGGGGCATTTCCCCCCTGTCCGGCACGGGGGGGATCGGCGCTTCCGCGCTGAGCCATGTGCTGTTTGCCGCAAAAA
AGCCCTCGCAAGTATCTTAT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MFAKLEGLEKKYLELEEALAQPDVFNDQDQYRKLTKAHADLSDVVELFRRHRAVSEELAENRLLLHDEDPEIRAMAQEEV
HRGEAELPNLEHELKVMLLPSDPLDEKNTILEIRAGTGGEEAALFAADLFRMYTRYAELKNWKVEIMSESPSENGGYKEI
ICLIAGDRVYSHLKFEAGTHRVQRVPATEAQGRIHTSAATVAVMPEAEEVDVEIRPEDLRIDIYRASGAGGQHVNKTESA
VRITHLPTNTVVTCQDERSQHKNKARAMKVLASRILAAERERQSSELSADRKAQVGSGDRSERIRTYNFPQGRCTDHRIN
LTLYSLDRIMEGELESLVEALGTAAQAEALKAQASE

Sequences:

>Translated_356_residues
MFAKLEGLEKKYLELEEALAQPDVFNDQDQYRKLTKAHADLSDVVELFRRHRAVSEELAENRLLLHDEDPEIRAMAQEEV
HRGEAELPNLEHELKVMLLPSDPLDEKNTILEIRAGTGGEEAALFAADLFRMYTRYAELKNWKVEIMSESPSENGGYKEI
ICLIAGDRVYSHLKFEAGTHRVQRVPATEAQGRIHTSAATVAVMPEAEEVDVEIRPEDLRIDIYRASGAGGQHVNKTESA
VRITHLPTNTVVTCQDERSQHKNKARAMKVLASRILAAERERQSSELSADRKAQVGSGDRSERIRTYNFPQGRCTDHRIN
LTLYSLDRIMEGELESLVEALGTAAQAEALKAQASE
>Mature_356_residues
MFAKLEGLEKKYLELEEALAQPDVFNDQDQYRKLTKAHADLSDVVELFRRHRAVSEELAENRLLLHDEDPEIRAMAQEEV
HRGEAELPNLEHELKVMLLPSDPLDEKNTILEIRAGTGGEEAALFAADLFRMYTRYAELKNWKVEIMSESPSENGGYKEI
ICLIAGDRVYSHLKFEAGTHRVQRVPATEAQGRIHTSAATVAVMPEAEEVDVEIRPEDLRIDIYRASGAGGQHVNKTESA
VRITHLPTNTVVTCQDERSQHKNKARAMKVLASRILAAERERQSSELSADRKAQVGSGDRSERIRTYNFPQGRCTDHRIN
LTLYSLDRIMEGELESLVEALGTAAQAEALKAQASE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=296, Percent_Identity=50, Blast_Score=301, Evalue=4e-82,
Organism=Homo sapiens, GI34577120, Length=357, Percent_Identity=38.9355742296919, Blast_Score=248, Evalue=5e-66,
Organism=Homo sapiens, GI166795305, Length=193, Percent_Identity=51.2953367875648, Blast_Score=214, Evalue=1e-55,
Organism=Escherichia coli, GI1787462, Length=353, Percent_Identity=57.2237960339943, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI2367172, Length=344, Percent_Identity=38.953488372093, Blast_Score=219, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17542784, Length=290, Percent_Identity=38.9655172413793, Blast_Score=197, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6321295, Length=316, Percent_Identity=42.7215189873418, Blast_Score=243, Evalue=4e-65,
Organism=Drosophila melanogaster, GI19921226, Length=320, Percent_Identity=40.9375, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_DESDA (B8J4R4)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002480872.1
- ProteinModelPortal:   B8J4R4
- GeneID:   7286017
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_2299
- HOGENOM:   HBG629764
- ProtClustDB:   PRK00591
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40054; Mature: 40054

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAKLEGLEKKYLELEEALAQPDVFNDQDQYRKLTKAHADLSDVVELFRRHRAVSEELAE
CCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
NRLLLHDEDPEIRAMAQEEVHRGEAELPNLEHELKVMLLPSDPLDEKNTILEIRAGTGGE
CCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCCC
EAALFAADLFRMYTRYAELKNWKVEIMSESPSENGGYKEIICLIAGDRVYSHLKFEAGTH
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEEEECCHHHHHEEECCCCH
RVQRVPATEAQGRIHTSAATVAVMPEAEEVDVEIRPEDLRIDIYRASGAGGQHVNKTESA
HHHCCCCCCCCCEEEECCEEEEECCCCHHCCEEECCCCEEEEEEEECCCCCCCCCCCCCE
VRITHLPTNTVVTCQDERSQHKNKARAMKVLASRILAAERERQSSELSADRKAQVGSGDR
EEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCC
SERIRTYNFPQGRCTDHRINLTLYSLDRIMEGELESLVEALGTAAQAEALKAQASE
CCCCEECCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
>Mature Secondary Structure
MFAKLEGLEKKYLELEEALAQPDVFNDQDQYRKLTKAHADLSDVVELFRRHRAVSEELAE
CCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
NRLLLHDEDPEIRAMAQEEVHRGEAELPNLEHELKVMLLPSDPLDEKNTILEIRAGTGGE
CCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCCC
EAALFAADLFRMYTRYAELKNWKVEIMSESPSENGGYKEIICLIAGDRVYSHLKFEAGTH
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEEEECCHHHHHEEECCCCH
RVQRVPATEAQGRIHTSAATVAVMPEAEEVDVEIRPEDLRIDIYRASGAGGQHVNKTESA
HHHCCCCCCCCCEEEECCEEEEECCCCHHCCEEECCCCEEEEEEEECCCCCCCCCCCCCE
VRITHLPTNTVVTCQDERSQHKNKARAMKVLASRILAAERERQSSELSADRKAQVGSGDR
EEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCC
SERIRTYNFPQGRCTDHRINLTLYSLDRIMEGELESLVEALGTAAQAEALKAQASE
CCCCEECCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA