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The map label for this gene is rimO [H]

Identifier: 220905544

GI number: 220905544

Start: 2748319

End: 2749647

Strand: Reverse

Name: rimO [H]

Synonym: Ddes_2283

Alternate gene names: 220905544

Gene position: 2749647-2748319 (Counterclockwise)

Preceding gene: 220905546

Following gene: 220905542

Centisome position: 95.69

GC content: 62.0

Gene sequence:

>1329_bases
ATGACAAGTGCAAACAAACAGAAAAAAATCAAGGTCTGGTCGCTGAGCCTGGGATGTCCCAAAAACAGGGTCGACAGCGA
GCGCCTGCTCGGTTCGCTGGGTGCGCCTGTGAGCCATGTGGAGCACATGGGCCGCGCTGATCTTGTATTCATCAATACCT
GTGGATTTATTGATCCCGCCGTGCGCGAATCCGTGCGCGCCGTTCTGGATGCGGGGCAGCGTCTGGGCCGTTGCAGCAAA
AAGCCTCTGCTGGCTGTGGGCGGCTGCATGGTGGGCCGTTACGGTGCGGAAAACCTGGCTGAAGATCTGCCCGAAGTGGA
TCTGTGGCTACCCACGGCTGATCTGGACCGCTGGCCCGGCATGGTGGCCGAGGCCCTGGCCCTGCCCACGCCGCCTGTGC
GTATGCCCGGCGGCGGCAGGCTGCTTTCCACGGGGCCTTCCTACGCATGGCTCAAGATAGGCGAAGGCTGCCGGCACAAA
TGTGCCTTTTGCACCATTCCCTCCATCAGGGGGGGGCTGCATTCGCTGCCTGCCGAATATATTGTGGACGAGGCCCGCGC
CCTGCTGGGGCAGGGAGTTCGCGAACTGGCCCTTGTGGCTCAGGATCTGACGGCCTGGGGTGCGGATCTGGGCATGAAAA
ACGGTCTTCCTTCCCTGCTGGAAAAACTTGCCGCGCTGGACGGTCTGGCCTGGCTGCGCCTGCTCTATCTGTACCCCAGC
GGAGTGACCCCGGAACTTCTTGCCCTCATACGCGATACCGGGGCACCGCTTTTGCCCTATCTGGATATTCCCCTGCAGCA
TGCCCACCCCGATGTACTCTCGCGCATGGGCAGGCCTTTTGCGGGCAACCCCCGGCGCGTGCTGGATGCCGTGCGGCAAA
ATTTGCCGCACGCGGCCCTGCGCACCACATTTATTGTGGGTTACCCCGGCGAGACGGATGAACAGTTTGAAAGCCTGTGC
CGCTTTGTGGAAGAAAGCGCTTTTCAGCATGTGGGGGTATTTGCCTATCAGGCTGAAGACGGCACAGAAGCGGCGACCTT
GCCGGATCAGGTGCCGGATGCGGTCAAGCAGTGGCGGCGCGATACTCTTATGGACATACAGGCAGATATCAGCGAACGCC
TGCTGGCGGCCCATGTGGGCAGTCGCATGCAGGTGCTGGTGGATGCGCCGCATCCCGAGTGGCCGGGCCTGCACAGCGGG
CGCGTGTGGCTTCAGGCCCCGGAGGTGGACGGCATCACCTATGTAAGCGGGCCGGGCGTCGCGCCCGGAGCGCTGGTGGA
ATGCGACATTGTGGAAAATACCGAATATGATCTGACTGCACTGGCCTAA

Upstream 100 bases:

>100_bases
GACGCCGGACTGTCAAGTCTTGAGCCTAGCACCGGACCCGGCGTTTTGCAAATGAGCTGCACTGGTGACAAGCCGCGGGC
ACATCTGTAGGATGCCCCTC

Downstream 100 bases:

>100_bases
GGGCTGTCTGCCATTGAAAATGTCTGGTTGCCCGGTGCGGTTTTTTCGGAAAATCCACACCACGGAGCTGTGCGAAGCGT
GACCGGCTTGCCGGGCATCC

Product: MiaB-like tRNA modifying protein YliG

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTSANKQKKIKVWSLSLGCPKNRVDSERLLGSLGAPVSHVEHMGRADLVFINTCGFIDPAVRESVRAVLDAGQRLGRCSK
KPLLAVGGCMVGRYGAENLAEDLPEVDLWLPTADLDRWPGMVAEALALPTPPVRMPGGGRLLSTGPSYAWLKIGEGCRHK
CAFCTIPSIRGGLHSLPAEYIVDEARALLGQGVRELALVAQDLTAWGADLGMKNGLPSLLEKLAALDGLAWLRLLYLYPS
GVTPELLALIRDTGAPLLPYLDIPLQHAHPDVLSRMGRPFAGNPRRVLDAVRQNLPHAALRTTFIVGYPGETDEQFESLC
RFVEESAFQHVGVFAYQAEDGTEAATLPDQVPDAVKQWRRDTLMDIQADISERLLAAHVGSRMQVLVDAPHPEWPGLHSG
RVWLQAPEVDGITYVSGPGVAPGALVECDIVENTEYDLTALA

Sequences:

>Translated_442_residues
MTSANKQKKIKVWSLSLGCPKNRVDSERLLGSLGAPVSHVEHMGRADLVFINTCGFIDPAVRESVRAVLDAGQRLGRCSK
KPLLAVGGCMVGRYGAENLAEDLPEVDLWLPTADLDRWPGMVAEALALPTPPVRMPGGGRLLSTGPSYAWLKIGEGCRHK
CAFCTIPSIRGGLHSLPAEYIVDEARALLGQGVRELALVAQDLTAWGADLGMKNGLPSLLEKLAALDGLAWLRLLYLYPS
GVTPELLALIRDTGAPLLPYLDIPLQHAHPDVLSRMGRPFAGNPRRVLDAVRQNLPHAALRTTFIVGYPGETDEQFESLC
RFVEESAFQHVGVFAYQAEDGTEAATLPDQVPDAVKQWRRDTLMDIQADISERLLAAHVGSRMQVLVDAPHPEWPGLHSG
RVWLQAPEVDGITYVSGPGVAPGALVECDIVENTEYDLTALA
>Mature_441_residues
TSANKQKKIKVWSLSLGCPKNRVDSERLLGSLGAPVSHVEHMGRADLVFINTCGFIDPAVRESVRAVLDAGQRLGRCSKK
PLLAVGGCMVGRYGAENLAEDLPEVDLWLPTADLDRWPGMVAEALALPTPPVRMPGGGRLLSTGPSYAWLKIGEGCRHKC
AFCTIPSIRGGLHSLPAEYIVDEARALLGQGVRELALVAQDLTAWGADLGMKNGLPSLLEKLAALDGLAWLRLLYLYPSG
VTPELLALIRDTGAPLLPYLDIPLQHAHPDVLSRMGRPFAGNPRRVLDAVRQNLPHAALRTTFIVGYPGETDEQFESLCR
FVEESAFQHVGVFAYQAEDGTEAATLPDQVPDAVKQWRRDTLMDIQADISERLLAAHVGSRMQVLVDAPHPEWPGLHSGR
VWLQAPEVDGITYVSGPGVAPGALVECDIVENTEYDLTALA

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI93277076, Length=397, Percent_Identity=28.7153652392947, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI28872782, Length=420, Percent_Identity=23.8095238095238, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI28872784, Length=459, Percent_Identity=23.0936819172113, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1787057, Length=457, Percent_Identity=36.980306345733, Blast_Score=258, Evalue=5e-70,
Organism=Escherichia coli, GI1786882, Length=443, Percent_Identity=27.9909706546275, Blast_Score=155, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17553146, Length=439, Percent_Identity=24.8291571753986, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI71996771, Length=242, Percent_Identity=31.404958677686, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922432, Length=376, Percent_Identity=25, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI21356207, Length=445, Percent_Identity=24.4943820224719, Blast_Score=105, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 47884; Mature: 47752

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSANKQKKIKVWSLSLGCPKNRVDSERLLGSLGAPVSHVEHMGRADLVFINTCGFIDPA
CCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHCCCCCEEEEECCCCCCHH
VRESVRAVLDAGQRLGRCSKKPLLAVGGCMVGRYGAENLAEDLPEVDLWLPTADLDRWPG
HHHHHHHHHHHHHHHHCCCCCCCEEECCHHHCCCCHHHHHHHCCCCEEECCCCCCHHCCH
MVAEALALPTPPVRMPGGGRLLSTGPSYAWLKIGEGCRHKCAFCTIPSIRGGLHSLPAEY
HHHHHHHCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCEEEECCHHHCCHHHCCHHH
IVDEARALLGQGVRELALVAQDLTAWGADLGMKNGLPSLLEKLAALDGLAWLRLLYLYPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GVTPELLALIRDTGAPLLPYLDIPLQHAHPDVLSRMGRPFAGNPRRVLDAVRQNLPHAAL
CCCHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHH
RTTFIVGYPGETDEQFESLCRFVEESAFQHVGVFAYQAEDGTEAATLPDQVPDAVKQWRR
HEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHHHHHHHHHHHH
DTLMDIQADISERLLAAHVGSRMQVLVDAPHPEWPGLHSGRVWLQAPEVDGITYVSGPGV
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCEEEECCCCC
APGALVECDIVENTEYDLTALA
CCCCEEEEEEECCCCCCEEECC
>Mature Secondary Structure 
TSANKQKKIKVWSLSLGCPKNRVDSERLLGSLGAPVSHVEHMGRADLVFINTCGFIDPA
CCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHCCCCCEEEEECCCCCCHH
VRESVRAVLDAGQRLGRCSKKPLLAVGGCMVGRYGAENLAEDLPEVDLWLPTADLDRWPG
HHHHHHHHHHHHHHHHCCCCCCCEEECCHHHCCCCHHHHHHHCCCCEEECCCCCCHHCCH
MVAEALALPTPPVRMPGGGRLLSTGPSYAWLKIGEGCRHKCAFCTIPSIRGGLHSLPAEY
HHHHHHHCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCEEEECCHHHCCHHHCCHHH
IVDEARALLGQGVRELALVAQDLTAWGADLGMKNGLPSLLEKLAALDGLAWLRLLYLYPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GVTPELLALIRDTGAPLLPYLDIPLQHAHPDVLSRMGRPFAGNPRRVLDAVRQNLPHAAL
CCCHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHH
RTTFIVGYPGETDEQFESLCRFVEESAFQHVGVFAYQAEDGTEAATLPDQVPDAVKQWRR
HEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHHHHHHHHHHHH
DTLMDIQADISERLLAAHVGSRMQVLVDAPHPEWPGLHSGRVWLQAPEVDGITYVSGPGV
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCEEEECCCCC
APGALVECDIVENTEYDLTALA
CCCCEEEEEEECCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA