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The map label for this gene is emrE [H]

Identifier: 220905334

GI number: 220905334

Start: 2492977

End: 2493321

Strand: Reverse

Name: emrE [H]

Synonym: Ddes_2072

Alternate gene names: 220905334

Gene position: 2493321-2492977 (Counterclockwise)

Preceding gene: 220905335

Following gene: 220905333

Centisome position: 86.77

GC content: 52.75

Gene sequence:

>345_bases
ATGCATCCCGCCACCTTGGCCTATCTGCAACTGGGCGGAGCCATTCTGCTTGAGGTCATTGCCACGTCATGCCTCAAGCA
GTCCGAGGGCATGAGCCGTCTTGTCCCCACTGTGACTGCCCTGGTCGGTTACGGCTTTTCATTTTTCCTGCTATCACTGG
TGCTCAAGGTTATACCCATGGGGGTTTCCTACGGCATCTGGAGTGGCGTGGGCATCGTTATTGTCTCTATCATCGGCCTT
GTTTTTTTCGGGCAAAAACTTGACATGGCGGCCTGCATAGGGCTTGGATTTATTGTTGTGGGCGTGCTTGTCATCAATTT
TTTTTCCAGCTCCGTGCCGCATTGA

Upstream 100 bases:

>100_bases
TTCTGGCGGTCATGTGCCGGGCGAGCATGCAGGCTTTGTACGCTTTCTCAATGTTGAAGCTCTAGTTGTGGCCGCTTATG
ACGGCCTGTATGAGGAAAGT

Downstream 100 bases:

>100_bases
TCACGAGCGGGCAACATGGCGCAATGATACGGTAAAAAGACATTGCCGACGTGACGCCAGGCGGTGTTTTCATGCCCGGA
TCACGGCTTTTCGCCGGGGG

Product: small multidrug resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Efflux-multidrug resistance protein emrE; Ethidium resistance protein; Methyl viologen resistance protein C [H]

Number of amino acids: Translated: 114; Mature: 114

Protein sequence:

>114_residues
MHPATLAYLQLGGAILLEVIATSCLKQSEGMSRLVPTVTALVGYGFSFFLLSLVLKVIPMGVSYGIWSGVGIVIVSIIGL
VFFGQKLDMAACIGLGFIVVGVLVINFFSSSVPH

Sequences:

>Translated_114_residues
MHPATLAYLQLGGAILLEVIATSCLKQSEGMSRLVPTVTALVGYGFSFFLLSLVLKVIPMGVSYGIWSGVGIVIVSIIGL
VFFGQKLDMAACIGLGFIVVGVLVINFFSSSVPH
>Mature_114_residues
MHPATLAYLQLGGAILLEVIATSCLKQSEGMSRLVPTVTALVGYGFSFFLLSLVLKVIPMGVSYGIWSGVGIVIVSIIGL
VFFGQKLDMAACIGLGFIVVGVLVINFFSSSVPH

Specific function: Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane of E.coli., thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistanc

COG id: COG2076

COG function: function code P; Membrane transporters of cations and cationic drugs

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the small multidrug resistance (SMR) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786755, Length=114, Percent_Identity=51.7543859649123, Blast_Score=114, Evalue=2e-27,
Organism=Escherichia coli, GI87082378, Length=88, Percent_Identity=35.2272727272727, Blast_Score=59, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000390 [H]

Pfam domain/function: PF00893 Multi_Drug_Res [H]

EC number: NA

Molecular weight: Translated: 11978; Mature: 11978

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPATLAYLQLGGAILLEVIATSCLKQSEGMSRLVPTVTALVGYGFSFFLLSLVLKVIPM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
GVSYGIWSGVGIVIVSIIGLVFFGQKLDMAACIGLGFIVVGVLVINFFSSSVPH
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHPATLAYLQLGGAILLEVIATSCLKQSEGMSRLVPTVTALVGYGFSFFLLSLVLKVIPM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
GVSYGIWSGVGIVIVSIIGLVFFGQKLDMAACIGLGFIVVGVLVINFFSSSVPH
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1936950; 1320256; 9278503; 7896833; 9050242; 9688273 [H]