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The map label for this gene is metF [H]

Identifier: 220905270

GI number: 220905270

Start: 2421703

End: 2422602

Strand: Reverse

Name: metF [H]

Synonym: Ddes_2008

Alternate gene names: 220905270

Gene position: 2422602-2421703 (Counterclockwise)

Preceding gene: 220905275

Following gene: 220905269

Centisome position: 84.31

GC content: 61.22

Gene sequence:

>900_bases
ATGCATATCGGAGACAGCATCCGCGCCGCCCAAAAGCCTTTTTTTTCGCTGGAGTTCTTTCCTCCGGCGGATGAGGCCAA
TCTGCCCGCCTTTTTTGAAACTGTTGAGCAGCTGCGCCCCCTGGCTCCCCTGTTTGCTTCCGTAACTTACGGCGCGGGAG
GCGGCAAGCAGCGCAACACACTGGCCGTGACGGCCGAGCTTGCGCGGCGCGGTCTGAACACTATGGCCCACCTGACCTGT
GTGGGGGCCGACCCCGAGAGCATCTCGACCTATCTTTCCGAACTGAGGGCCGCTGGTGTGGACAACGTGCTGGCTCTTCG
TGGTGACCCGCCCGCCGGAGAAGAGTGGGACTGGAACAGCGGCCGCTTCCGGCACGCATCTGATCTGGTGACGTTTGCCC
GTCGGCAGCAGCCCTATATGGGCATTGGCGTGGCGGCTTATCCCGCGCCGCATCCCGAGTCTACTTCCATTTCGCAGGAC
CGTCGCCATACCGCCGATAAAATGCGCAGCGGCGCTGATTTTGCCGTGACCCAGCTTTTTTTCGATGCCCGCGAATATGC
CGACCTGCTGGAGCATCTGCGCGGTCAGGGCATCCACACGCCTGTTATTCCGGGCATTCTGCCCATACAGAGCTTTGCCT
CGCTACGGCGGGTGCTTGCGTTGTGCGGGGCCAATATTCCCGGCAAGCTCTACCTTGATCTTGAAAAGGCTGATGAAAAG
GGCGGGGTAGAGGCCGTGCGCGAGGTGGGGCTGGAATTTGCCGTGCGCCAGATACGCTTTTTGCTGGATAACGGTGCCCC
AGGGGTCCACCTGTACACCCTGAACAAGGCCGACATGTGTTTGCGCCTCGCGCAGGAATCCGGCCTGCTTGGGCCTGATG
TGAATCTGCCTGCGGCCTGA

Upstream 100 bases:

>100_bases
GTGGGGCTTTTTTTCTTGACATATCAATTTATCTTGATATAAACATAAAATAGATTCGCACCCGTATGGGTCGCACCCTG
CTGCCACAAGGAGTTGCCGC

Downstream 100 bases:

>100_bases
TGCGCTCGCGCCCGCGTGGGTAAGGTCGGCCCCGCGTGCTTCTTGCCGGGAGGGCAGCCCTGCGTGCTTCGGCCCAGATG
CCCCGGCCGTATTGACCTTG

Product: methylenetetrahydrofolate reductase (NAD(P)H)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MHIGDSIRAAQKPFFSLEFFPPADEANLPAFFETVEQLRPLAPLFASVTYGAGGGKQRNTLAVTAELARRGLNTMAHLTC
VGADPESISTYLSELRAAGVDNVLALRGDPPAGEEWDWNSGRFRHASDLVTFARRQQPYMGIGVAAYPAPHPESTSISQD
RRHTADKMRSGADFAVTQLFFDAREYADLLEHLRGQGIHTPVIPGILPIQSFASLRRVLALCGANIPGKLYLDLEKADEK
GGVEAVREVGLEFAVRQIRFLLDNGAPGVHLYTLNKADMCLRLAQESGLLGPDVNLPAA

Sequences:

>Translated_299_residues
MHIGDSIRAAQKPFFSLEFFPPADEANLPAFFETVEQLRPLAPLFASVTYGAGGGKQRNTLAVTAELARRGLNTMAHLTC
VGADPESISTYLSELRAAGVDNVLALRGDPPAGEEWDWNSGRFRHASDLVTFARRQQPYMGIGVAAYPAPHPESTSISQD
RRHTADKMRSGADFAVTQLFFDAREYADLLEHLRGQGIHTPVIPGILPIQSFASLRRVLALCGANIPGKLYLDLEKADEK
GGVEAVREVGLEFAVRQIRFLLDNGAPGVHLYTLNKADMCLRLAQESGLLGPDVNLPAA
>Mature_299_residues
MHIGDSIRAAQKPFFSLEFFPPADEANLPAFFETVEQLRPLAPLFASVTYGAGGGKQRNTLAVTAELARRGLNTMAHLTC
VGADPESISTYLSELRAAGVDNVLALRGDPPAGEEWDWNSGRFRHASDLVTFARRQQPYMGIGVAAYPAPHPESTSISQD
RRHTADKMRSGADFAVTQLFFDAREYADLLEHLRGQGIHTPVIPGILPIQSFASLRRVLALCGANIPGKLYLDLEKADEK
GGVEAVREVGLEFAVRQIRFLLDNGAPGVHLYTLNKADMCLRLAQESGLLGPDVNLPAA

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=291, Percent_Identity=33.6769759450172, Blast_Score=168, Evalue=6e-42,
Organism=Escherichia coli, GI1790377, Length=276, Percent_Identity=32.9710144927536, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI25147436, Length=287, Percent_Identity=35.8885017421603, Blast_Score=171, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI25147441, Length=287, Percent_Identity=35.8885017421603, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6325234, Length=314, Percent_Identity=34.3949044585987, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6321313, Length=293, Percent_Identity=30.7167235494881, Blast_Score=144, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 32491; Mature: 32491

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIGDSIRAAQKPFFSLEFFPPADEANLPAFFETVEQLRPLAPLFASVTYGAGGGKQRNT
CCCCCHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
LAVTAELARRGLNTMAHLTCVGADPESISTYLSELRAAGVDNVLALRGDPPAGEEWDWNS
HEEEHHHHHCCCCHHHHEEEECCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCC
GRFRHASDLVTFARRQQPYMGIGVAAYPAPHPESTSISQDRRHTADKMRSGADFAVTQLF
CCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
FDAREYADLLEHLRGQGIHTPVIPGILPIQSFASLRRVLALCGANIPGKLYLDLEKADEK
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
GGVEAVREVGLEFAVRQIRFLLDNGAPGVHLYTLNKADMCLRLAQESGLLGPDVNLPAA
CHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MHIGDSIRAAQKPFFSLEFFPPADEANLPAFFETVEQLRPLAPLFASVTYGAGGGKQRNT
CCCCCHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
LAVTAELARRGLNTMAHLTCVGADPESISTYLSELRAAGVDNVLALRGDPPAGEEWDWNS
HEEEHHHHHCCCCHHHHEEEECCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCC
GRFRHASDLVTFARRQQPYMGIGVAAYPAPHPESTSISQDRRHTADKMRSGADFAVTQLF
CCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
FDAREYADLLEHLRGQGIHTPVIPGILPIQSFASLRRVLALCGANIPGKLYLDLEKADEK
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
GGVEAVREVGLEFAVRQIRFLLDNGAPGVHLYTLNKADMCLRLAQESGLLGPDVNLPAA
CHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]