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The map label for this gene is trmE [H]

Identifier: 220905227

GI number: 220905227

Start: 2368591

End: 2369997

Strand: Reverse

Name: trmE [H]

Synonym: Ddes_1965

Alternate gene names: 220905227

Gene position: 2369997-2368591 (Counterclockwise)

Preceding gene: 220905228

Following gene: 220905226

Centisome position: 82.48

GC content: 62.19

Gene sequence:

>1407_bases
ATGAGCACCAACGACACCATAGCAGCCATTGCCACGCCCCCCGGATCCGGCGGGGTCGGCATTGTGCGTATTTCCGGCCC
CGAAGCCAAGCAGACGCTCGCGCGCATGTTTTTGCCCCTGTCGCCGAAATTTGAAAACTTTCGCCCCTGGTTTCTGCACA
GGGGACGCGTCGTGGACTGGAACGGGGAAAACCTGGATGACGTGCTTGCTGTCTTTATGCCCGGTCCGCGCAGTTTTACC
GGCGAAGATGTGGCTGAAATACATTGTCATGGCGGCCCGTTTATTGTGCAGTCAGTGCTTGAGGCTGCCTTGCGTCTCGG
GGCGCGCCAGGCACAGCAGGGCGAATTTTCGCGTCGTGCCTTTATCAACGGGCGTATGGACCTGAGCCAGGCTGAAGCTG
TGGCCGAACTGATTGCCGCGCCTTCGCGCGAAGCCCTGCGTTACGGCCTGAACCGGCTGGACGGGCTGCTGGCCCGCCGG
ACGGAGACTCTGCGCCGCGAGCTTGAAGACCTGCGCATGCAGGTCTGCGTGGCCGTGGATTTTCCCGAGGACGAAGTGGA
AGGCATGGATGCGCCCACGTTCAGCGCGGCTGTGGAAAAAGTGGCTTCGGCCTTGCGTCATCTGCTTCAGGGCAGCCGCA
GGGCGCGGGTCATGCAGCAGGGTGCTGTGGTTGTGCTGGCCGGGGCGGTCAATGCGGGCAAGTCCAGTCTCATGAACGCG
CTGCTTGGGCGCAACCGCGCCCTGGTTACCGATATTCCCGGTACAACGCGGGACTTTCTTGAAGAAGCCTGCGATCTTGG
CGGCCTGCCCGTGCGGCTGACAGATACTGCCGGCTTGCGCAAAGCTGCAGAAAGTATTGAAGAAATGGGCGTTGAGCGCA
GCAGGCAAAAACTGGCTGAAGCCGATGCTGTTGTTTTGGTGCTTGACGGCGGCGTGTTGGGAGAAGCCGGAGCCGCTGCC
GCGACCTGCCCTGACCCCGCTGCCCGGGAAGTTCTGGAGCTTGCCGGTGCTACACCTGTGCTGGTTGTATGGAACAAATG
CGATCTGTGCACGCCTTCGGTTTTTCCGCCTCGCTGGATTCAGGGGCAAACCTGTTGCACAACAAGCGCTTTTTCCGATA
CACAGGTGGAGGACATGGCCGAAAGCTTGCGCGCCCTGCTGCTGGCCGGCGGCGGGGGAGCCGCCGCAGAAGAGGGGCTT
GCTCCCAATGCGCGGCAAAGCATGGCACTGGAAGCGGCCCTGGCCGAACTGGCGGCCCTGCGGGAAGATGTGGATGCCGG
GCGGTCGTATGACTGTTGCGCCGTGCGCCTGGACGTGGCTGCCGCCCATCTGGCGGAGGTAACAGGCCTGAGCAGCCCCG
CTGAGGTGCTGGACAGGGTTTTTTCTCAATTTTGTATCGGCAAGTAG

Upstream 100 bases:

>100_bases
TCACTTTTTTCGCCCTGCGCGAAAGGCGGAGGCCGCATTTTTTGAACTTTCACGCCGCGCACGGCGGCACGCCCGGCGAT
CAGCGGGGCAGTACGACATC

Downstream 100 bases:

>100_bases
TCTGGTAAGTAATTTATGGATATGGATGTTCTGCGCCGCCGCCTGAGCAGCGAAGAGATAAACGCCTTGCCCCTGTGTCA
CTACGAGGGGCCGGTGCATG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSTNDTIAAIATPPGSGGVGIVRISGPEAKQTLARMFLPLSPKFENFRPWFLHRGRVVDWNGENLDDVLAVFMPGPRSFT
GEDVAEIHCHGGPFIVQSVLEAALRLGARQAQQGEFSRRAFINGRMDLSQAEAVAELIAAPSREALRYGLNRLDGLLARR
TETLRRELEDLRMQVCVAVDFPEDEVEGMDAPTFSAAVEKVASALRHLLQGSRRARVMQQGAVVVLAGAVNAGKSSLMNA
LLGRNRALVTDIPGTTRDFLEEACDLGGLPVRLTDTAGLRKAAESIEEMGVERSRQKLAEADAVVLVLDGGVLGEAGAAA
ATCPDPAAREVLELAGATPVLVVWNKCDLCTPSVFPPRWIQGQTCCTTSAFSDTQVEDMAESLRALLLAGGGGAAAEEGL
APNARQSMALEAALAELAALREDVDAGRSYDCCAVRLDVAAAHLAEVTGLSSPAEVLDRVFSQFCIGK

Sequences:

>Translated_468_residues
MSTNDTIAAIATPPGSGGVGIVRISGPEAKQTLARMFLPLSPKFENFRPWFLHRGRVVDWNGENLDDVLAVFMPGPRSFT
GEDVAEIHCHGGPFIVQSVLEAALRLGARQAQQGEFSRRAFINGRMDLSQAEAVAELIAAPSREALRYGLNRLDGLLARR
TETLRRELEDLRMQVCVAVDFPEDEVEGMDAPTFSAAVEKVASALRHLLQGSRRARVMQQGAVVVLAGAVNAGKSSLMNA
LLGRNRALVTDIPGTTRDFLEEACDLGGLPVRLTDTAGLRKAAESIEEMGVERSRQKLAEADAVVLVLDGGVLGEAGAAA
ATCPDPAAREVLELAGATPVLVVWNKCDLCTPSVFPPRWIQGQTCCTTSAFSDTQVEDMAESLRALLLAGGGGAAAEEGL
APNARQSMALEAALAELAALREDVDAGRSYDCCAVRLDVAAAHLAEVTGLSSPAEVLDRVFSQFCIGK
>Mature_467_residues
STNDTIAAIATPPGSGGVGIVRISGPEAKQTLARMFLPLSPKFENFRPWFLHRGRVVDWNGENLDDVLAVFMPGPRSFTG
EDVAEIHCHGGPFIVQSVLEAALRLGARQAQQGEFSRRAFINGRMDLSQAEAVAELIAAPSREALRYGLNRLDGLLARRT
ETLRRELEDLRMQVCVAVDFPEDEVEGMDAPTFSAAVEKVASALRHLLQGSRRARVMQQGAVVVLAGAVNAGKSSLMNAL
LGRNRALVTDIPGTTRDFLEEACDLGGLPVRLTDTAGLRKAAESIEEMGVERSRQKLAEADAVVLVLDGGVLGEAGAAAA
TCPDPAAREVLELAGATPVLVVWNKCDLCTPSVFPPRWIQGQTCCTTSAFSDTQVEDMAESLRALLLAGGGGAAAEEGLA
PNARQSMALEAALAELAALREDVDAGRSYDCCAVRLDVAAAHLAEVTGLSSPAEVLDRVFSQFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=472, Percent_Identity=34.1101694915254, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI193082988, Length=507, Percent_Identity=33.1360946745562, Blast_Score=206, Evalue=5e-53,
Organism=Homo sapiens, GI193082993, Length=466, Percent_Identity=33.4763948497854, Blast_Score=200, Evalue=3e-51,
Organism=Escherichia coli, GI2367268, Length=470, Percent_Identity=38.7234042553192, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI87082120, Length=87, Percent_Identity=39.0804597701149, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17507259, Length=469, Percent_Identity=28.1449893390192, Blast_Score=167, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6323665, Length=503, Percent_Identity=29.2246520874751, Blast_Score=188, Evalue=2e-48,
Organism=Drosophila melanogaster, GI45550813, Length=480, Percent_Identity=30, Blast_Score=187, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49686; Mature: 49555

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNDTIAAIATPPGSGGVGIVRISGPEAKQTLARMFLPLSPKFENFRPWFLHRGRVVDW
CCCCCCEEEEECCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHEECCEEEEC
NGENLDDVLAVFMPGPRSFTGEDVAEIHCHGGPFIVQSVLEAALRLGARQAQQGEFSRRA
CCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCHHHHHCCCHHHHH
FINGRMDLSQAEAVAELIAAPSREALRYGLNRLDGLLARRTETLRRELEDLRMQVCVAVD
HHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEE
FPEDEVEGMDAPTFSAAVEKVASALRHLLQGSRRARVMQQGAVVVLAGAVNAGKSSLMNA
CCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECCCCCHHHHHHH
LLGRNRALVTDIPGTTRDFLEEACDLGGLPVRLTDTAGLRKAAESIEEMGVERSRQKLAE
HHCCCCEEEECCCCCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHC
ADAVVLVLDGGVLGEAGAAAATCPDPAAREVLELAGATPVLVVWNKCDLCTPSVFPPRWI
CCEEEEEECCCEECCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCC
QGQTCCTTSAFSDTQVEDMAESLRALLLAGGGGAAAEEGLAPNARQSMALEAALAELAAL
CCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHH
REDVDAGRSYDCCAVRLDVAAAHLAEVTGLSSPAEVLDRVFSQFCIGK
HHHHHCCCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STNDTIAAIATPPGSGGVGIVRISGPEAKQTLARMFLPLSPKFENFRPWFLHRGRVVDW
CCCCCEEEEECCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHEECCEEEEC
NGENLDDVLAVFMPGPRSFTGEDVAEIHCHGGPFIVQSVLEAALRLGARQAQQGEFSRRA
CCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCHHHHHCCCHHHHH
FINGRMDLSQAEAVAELIAAPSREALRYGLNRLDGLLARRTETLRRELEDLRMQVCVAVD
HHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEE
FPEDEVEGMDAPTFSAAVEKVASALRHLLQGSRRARVMQQGAVVVLAGAVNAGKSSLMNA
CCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECCCCCHHHHHHH
LLGRNRALVTDIPGTTRDFLEEACDLGGLPVRLTDTAGLRKAAESIEEMGVERSRQKLAE
HHCCCCEEEECCCCCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHC
ADAVVLVLDGGVLGEAGAAAATCPDPAAREVLELAGATPVLVVWNKCDLCTPSVFPPRWI
CCEEEEEECCCEECCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCC
QGQTCCTTSAFSDTQVEDMAESLRALLLAGGGGAAAEEGLAPNARQSMALEAALAELAAL
CCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHH
REDVDAGRSYDCCAVRLDVAAAHLAEVTGLSSPAEVLDRVFSQFCIGK
HHHHHCCCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA