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The map label for this gene is pit [H]

Identifier: 220905222

GI number: 220905222

Start: 2363336

End: 2364340

Strand: Reverse

Name: pit [H]

Synonym: Ddes_1960

Alternate gene names: 220905222

Gene position: 2364340-2363336 (Counterclockwise)

Preceding gene: 220905223

Following gene: 220905216

Centisome position: 82.28

GC content: 59.8

Gene sequence:

>1005_bases
ATGTTTGATATTCCTGTGCTGCTGGCGCTCATCGTGCTGGTGGCCCTGATATTCGACTTTACCAACGGCGCTCACGACTG
CGCCAACGCCATCGCCACCGTGGTATCCACCAAGGTGGTCACGCCGCGTTTTGCCGTGGGAGCCGCCGCCCTGCTGAATC
TGGGCGGCGCTCTGCTTGGAACGGAAGTCGCCAAGACGCTGGGCAGCGGCATCGTGCTGCCCCATGTGGTCGAGGGCAGC
CATGTGCTTGTGCTGGCTGCGCTGGTGGGGGCCATAAGCTGGAACTGCATAACCTGGTATTTCGGCATTCCCTCTTCTTC
TTCCCACGCCCTTATTGGCGGGCTGATCGGTGCGGCAGTGGCCGATGCCGGTTTTAGCGCGCTCAACGGCGGGGGCATTG
TGGACAAGGTGCTTATCCCGCTGATAGCCTCGCCTCTGGCCGGTTTTCTGGTGGGTTACCTGATTATGTGGATCATCTTC
TGGACTTGCGCCCGCATGCACCGGCGTAAGGTCAACGGCGTTTTCCGGCGGTTGCAGCTTGTGTCAGCAGCCTTTATGGC
TACCAGCCACGGCCTCAACGATGCCCAGAAAACCATGGGCATCATTACCCTGGCCCTGCTTATTTTCGGCAAGATTGATG
TTGTGGAAGTGCCGTTGTGGGTCAAGCTTTCCTGCGCCGGGGCCATGGCCCTGGGAACCGCTGTGGGCGGCTGGAAGATC
GTGAAAACTATGGGCCATCGCATTTTCAAGCTGGAACCGGTGCACGGCTTTGCCGCTGAGACTTCGGCGGCGCTGGTCAT
TACGGGAGCCTCCATGCTGGGCGCTCCCGTAAGTACCACCCACACCATTTCCGCATGCATTTTTGGCGTCGGTTCCACCA
AGCGTCTTTCTGCGGTGCGCTGGAATGTGGCCGGAAGCCTGGTCACTGCCTGGGTGCTTACCCTGCCCGCCGCGGGCGGC
ATCGGCTTTATCTCTTACTGGTTGCTGCATTTTATCTGGAACTAG

Upstream 100 bases:

>100_bases
ATGCGGACGCTCAAATGGAGCCAGGCGTACGAGCGCATGAGCATCCTGCTTGAACAGGTCAATGCGCTGGCGGAAACCAT
CGAGGAAGCGGTGCTGAAAA

Downstream 100 bases:

>100_bases
CGCCGGCAAGCGCCGGGTAAAAAATTCTGCTGTGAGCGTACCCCCTGAAGCAGGGGGTACGCTCACATTTTTTTGTTGCA
AAGGTATATCTTTTTCGCGG

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MFDIPVLLALIVLVALIFDFTNGAHDCANAIATVVSTKVVTPRFAVGAAALLNLGGALLGTEVAKTLGSGIVLPHVVEGS
HVLVLAALVGAISWNCITWYFGIPSSSSHALIGGLIGAAVADAGFSALNGGGIVDKVLIPLIASPLAGFLVGYLIMWIIF
WTCARMHRRKVNGVFRRLQLVSAAFMATSHGLNDAQKTMGIITLALLIFGKIDVVEVPLWVKLSCAGAMALGTAVGGWKI
VKTMGHRIFKLEPVHGFAAETSAALVITGASMLGAPVSTTHTISACIFGVGSTKRLSAVRWNVAGSLVTAWVLTLPAAGG
IGFISYWLLHFIWN

Sequences:

>Translated_334_residues
MFDIPVLLALIVLVALIFDFTNGAHDCANAIATVVSTKVVTPRFAVGAAALLNLGGALLGTEVAKTLGSGIVLPHVVEGS
HVLVLAALVGAISWNCITWYFGIPSSSSHALIGGLIGAAVADAGFSALNGGGIVDKVLIPLIASPLAGFLVGYLIMWIIF
WTCARMHRRKVNGVFRRLQLVSAAFMATSHGLNDAQKTMGIITLALLIFGKIDVVEVPLWVKLSCAGAMALGTAVGGWKI
VKTMGHRIFKLEPVHGFAAETSAALVITGASMLGAPVSTTHTISACIFGVGSTKRLSAVRWNVAGSLVTAWVLTLPAAGG
IGFISYWLLHFIWN
>Mature_334_residues
MFDIPVLLALIVLVALIFDFTNGAHDCANAIATVVSTKVVTPRFAVGAAALLNLGGALLGTEVAKTLGSGIVLPHVVEGS
HVLVLAALVGAISWNCITWYFGIPSSSSHALIGGLIGAAVADAGFSALNGGGIVDKVLIPLIASPLAGFLVGYLIMWIIF
WTCARMHRRKVNGVFRRLQLVSAAFMATSHGLNDAQKTMGIITLALLIFGKIDVVEVPLWVKLSCAGAMALGTAVGGWKI
VKTMGHRIFKLEPVHGFAAETSAALVITGASMLGAPVSTTHTISACIFGVGSTKRLSAVRWNVAGSLVTAWVLTLPAAGG
IGFISYWLLHFIWN

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=157, Percent_Identity=30.5732484076433, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI5803173, Length=152, Percent_Identity=30.9210526315789, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1789907, Length=220, Percent_Identity=35.4545454545455, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1789360, Length=220, Percent_Identity=33.1818181818182, Blast_Score=99, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI25146401, Length=170, Percent_Identity=29.4117647058824, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71981576, Length=170, Percent_Identity=29.4117647058824, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI32566716, Length=206, Percent_Identity=28.1553398058252, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17539280, Length=163, Percent_Identity=30.0613496932515, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17536725, Length=179, Percent_Identity=24.0223463687151, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319773, Length=161, Percent_Identity=31.055900621118, Blast_Score=82, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 34821; Mature: 34821

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDIPVLLALIVLVALIFDFTNGAHDCANAIATVVSTKVVTPRFAVGAAALLNLGGALLG
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHH
TEVAKTLGSGIVLPHVVEGSHVLVLAALVGAISWNCITWYFGIPSSSSHALIGGLIGAAV
HHHHHHHCCCCEECEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHH
ADAGFSALNGGGIVDKVLIPLIASPLAGFLVGYLIMWIIFWTCARMHRRKVNGVFRRLQL
HHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSAAFMATSHGLNDAQKTMGIITLALLIFGKIDVVEVPLWVKLSCAGAMALGTAVGGWKI
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEECCCEEEEECCHHHHHHHHCCHHHH
VKTMGHRIFKLEPVHGFAAETSAALVITGASMLGAPVSTTHTISACIFGVGSTKRLSAVR
HHHHCCCEEEECCCCCCCCCCCCEEEEECHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHH
WNVAGSLVTAWVLTLPAAGGIGFISYWLLHFIWN
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MFDIPVLLALIVLVALIFDFTNGAHDCANAIATVVSTKVVTPRFAVGAAALLNLGGALLG
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHH
TEVAKTLGSGIVLPHVVEGSHVLVLAALVGAISWNCITWYFGIPSSSSHALIGGLIGAAV
HHHHHHHCCCCEECEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHH
ADAGFSALNGGGIVDKVLIPLIASPLAGFLVGYLIMWIIFWTCARMHRRKVNGVFRRLQL
HHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSAAFMATSHGLNDAQKTMGIITLALLIFGKIDVVEVPLWVKLSCAGAMALGTAVGGWKI
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEECCCEEEEECCHHHHHHHHCCHHHH
VKTMGHRIFKLEPVHGFAAETSAALVITGASMLGAPVSTTHTISACIFGVGSTKRLSAVR
HHHHCCCEEEECCCCCCCCCCCCEEEEECHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHH
WNVAGSLVTAWVLTLPAAGGIGFISYWLLHFIWN
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]