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The map label for this gene is appA [H]

Identifier: 220904806

GI number: 220904806

Start: 1836812

End: 1838503

Strand: Reverse

Name: appA [H]

Synonym: Ddes_1538

Alternate gene names: 220904806

Gene position: 1838503-1836812 (Counterclockwise)

Preceding gene: 220904818

Following gene: 220904804

Centisome position: 63.98

GC content: 56.56

Gene sequence:

>1692_bases
ATGAAAAAAATAAATTTTGAGTGGGTAAACAGTATGTTGGATGTATTTCTTCCAATTCGTATCCTGCTCTTACTGACTGT
TTTTATGGGATTTTTTTCGCCCCTTGCCGCCGTGGCCTCCGTAACCGGGGCTGACGCAGCAAAAAAAGAAAATTTTGAAA
CGCCCGTGGACGGAGGCCGTATCCTTTTCGGCAGCATTGGTGAAGCCTCCAACCTTATCCCCTACCTTTCGGCTGACTCC
GCTTCACACGAGGTCGCGGGGCTTATTTATGTGGCGCCCCTGCGTTATGACAAGGACCTTCAGGTAGAACCCTGGGCCGC
AGAAAGCTGGAGTATGGAAGACGAAGGGCGGCTTATGCGCTTTACCCTGCGCAAGGGGATCCTGTGGGAAGACGGTGTCG
AGCTGACTGCCGAGGACGTGGCCTATACCTGCAAGGTAGTGGCGGATCCGGCCACGGGAAGCCCCTATGCCGAAGATTTT
TCGCGCATCAAGGAACTCCGGGTTATTGACCGCTATACGTTTGAAGTCCGTTACGAGCATTTTTTTGCGCGCGCCGTTTC
TTCGTGGATGCAGCCCATATTGCCCCGGCATCTGCTTGATGGGCAGGATATCCGCAACAGCCCGCTCACACGCAAGCCGG
TGGGGGCGGGACCGTACAGGCTCAAGGCCTGGGAGCCGGGCAGCCGTATTATTCTTGAGGCTTCGCCCACCTATTTTGAT
GGCAAGCCCTACATAACCGAAGCCGTGTACCGCATCATTCCCGATGGCGCGACCATGTTTATGGAGGCGCGCGCCGGGCG
GCTGGACATTATGGATCTTTCTCCCCTGCAGTATCTGCGGCAGACCTCCGGCCCCATGTGGGAGCGCGAGTTCAACAAGT
TTCGCTATCTTGCCTCGGCCTACGTGTTTCTGGGCTTTAACCTGCAACATCCGTTTTTTGGGGATGTGCGGGTGCGCAGG
GCCATTTCCCAGGCCATCGACCGGGACAGCATCATCCAGGGGGTGCTGCTCGGGCAGGGCATACCGGCCTTCGGGCCTTT
CAAGCCCGGGGTCTGGGCCTATCATCCGACCCTGAAGCCCATACCGCAAAATGTGGCCGCCGCACGCGCCCTGCTGGCCG
AGGCCGGTTTTACCGACAGCGATGGCGACGGCCTGCTGGACAGGGACGGCAAGCCCTTTTCTTTTACCATACTGACCAAT
CAGGGCAACGAGCAGCGTATCCTCACGGCCACGGTCATACAGGCGCAGCTCAGAGCCATCGGCATTGACGTGCATATCCG
CACGGTGGAGTGGGCGGCCTTTATCCGCGAATTTGTCAACAAGGGCCGTTTTGACGCCATTATTCTTGGCTGGACCATTC
CACAGGACCCGGATATCTATCAGGTATGGCACTCATCGCAGGCTGTTGAAGGCGGCCTTAACTTTACCTACTATAAAAAT
CCCGAAGTGGACAGGTTGCTGGAAGAAGCGCGCTCCATCCCCGATCAGAGCACGCGCGCCCGCCTGTACTGTCGCATTCA
GGAAATACTGGATGCGGAACAGCCCTACTGTTTTTTGTATGTTCCCTACGCGCTGCCTGTGGTGCAGAAGCGTTTTCAGG
GCGTCAAGCCGGCTCTGGCTGGCCTGATGTACAATTTTGAAAAGTGGTGGGTTCCGAAAGAATTACAACGGCACAGCCTC
TCGGCGGACTAG

Upstream 100 bases:

>100_bases
CTGTCAGGTGTATCGAAAATTTCAATGACAGGGCGATGTTATGATGTTTGCGATAAATAGCACAGGTTGGGGGGCGAAAG
CAATATGAAGCATCAAAATT

Downstream 100 bases:

>100_bases
AGGAGATTATCTTTGAAGTGCTTCACAGTTCAAAGGTGTCATTCTGTCTAAAAATACAGTTTTGGGGCAGAATCTCCGCC
GTGTTACGTTGTGCTGCATT

Product: family 5 extracellular solute-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MKKINFEWVNSMLDVFLPIRILLLLTVFMGFFSPLAAVASVTGADAAKKENFETPVDGGRILFGSIGEASNLIPYLSADS
ASHEVAGLIYVAPLRYDKDLQVEPWAAESWSMEDEGRLMRFTLRKGILWEDGVELTAEDVAYTCKVVADPATGSPYAEDF
SRIKELRVIDRYTFEVRYEHFFARAVSSWMQPILPRHLLDGQDIRNSPLTRKPVGAGPYRLKAWEPGSRIILEASPTYFD
GKPYITEAVYRIIPDGATMFMEARAGRLDIMDLSPLQYLRQTSGPMWEREFNKFRYLASAYVFLGFNLQHPFFGDVRVRR
AISQAIDRDSIIQGVLLGQGIPAFGPFKPGVWAYHPTLKPIPQNVAAARALLAEAGFTDSDGDGLLDRDGKPFSFTILTN
QGNEQRILTATVIQAQLRAIGIDVHIRTVEWAAFIREFVNKGRFDAIILGWTIPQDPDIYQVWHSSQAVEGGLNFTYYKN
PEVDRLLEEARSIPDQSTRARLYCRIQEILDAEQPYCFLYVPYALPVVQKRFQGVKPALAGLMYNFEKWWVPKELQRHSL
SAD

Sequences:

>Translated_563_residues
MKKINFEWVNSMLDVFLPIRILLLLTVFMGFFSPLAAVASVTGADAAKKENFETPVDGGRILFGSIGEASNLIPYLSADS
ASHEVAGLIYVAPLRYDKDLQVEPWAAESWSMEDEGRLMRFTLRKGILWEDGVELTAEDVAYTCKVVADPATGSPYAEDF
SRIKELRVIDRYTFEVRYEHFFARAVSSWMQPILPRHLLDGQDIRNSPLTRKPVGAGPYRLKAWEPGSRIILEASPTYFD
GKPYITEAVYRIIPDGATMFMEARAGRLDIMDLSPLQYLRQTSGPMWEREFNKFRYLASAYVFLGFNLQHPFFGDVRVRR
AISQAIDRDSIIQGVLLGQGIPAFGPFKPGVWAYHPTLKPIPQNVAAARALLAEAGFTDSDGDGLLDRDGKPFSFTILTN
QGNEQRILTATVIQAQLRAIGIDVHIRTVEWAAFIREFVNKGRFDAIILGWTIPQDPDIYQVWHSSQAVEGGLNFTYYKN
PEVDRLLEEARSIPDQSTRARLYCRIQEILDAEQPYCFLYVPYALPVVQKRFQGVKPALAGLMYNFEKWWVPKELQRHSL
SAD
>Mature_563_residues
MKKINFEWVNSMLDVFLPIRILLLLTVFMGFFSPLAAVASVTGADAAKKENFETPVDGGRILFGSIGEASNLIPYLSADS
ASHEVAGLIYVAPLRYDKDLQVEPWAAESWSMEDEGRLMRFTLRKGILWEDGVELTAEDVAYTCKVVADPATGSPYAEDF
SRIKELRVIDRYTFEVRYEHFFARAVSSWMQPILPRHLLDGQDIRNSPLTRKPVGAGPYRLKAWEPGSRIILEASPTYFD
GKPYITEAVYRIIPDGATMFMEARAGRLDIMDLSPLQYLRQTSGPMWEREFNKFRYLASAYVFLGFNLQHPFFGDVRVRR
AISQAIDRDSIIQGVLLGQGIPAFGPFKPGVWAYHPTLKPIPQNVAAARALLAEAGFTDSDGDGLLDRDGKPFSFTILTN
QGNEQRILTATVIQAQLRAIGIDVHIRTVEWAAFIREFVNKGRFDAIILGWTIPQDPDIYQVWHSSQAVEGGLNFTYYKN
PEVDRLLEEARSIPDQSTRARLYCRIQEILDAEQPYCFLYVPYALPVVQKRFQGVKPALAGLMYNFEKWWVPKELQRHSL
SAD

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=482, Percent_Identity=26.7634854771784, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1789966, Length=464, Percent_Identity=25.8620689655172, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1787762, Length=502, Percent_Identity=25.8964143426295, Blast_Score=142, Evalue=8e-35,
Organism=Escherichia coli, GI1787052, Length=478, Percent_Identity=24.6861924686192, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1789397, Length=498, Percent_Identity=26.9076305220884, Blast_Score=125, Evalue=8e-30,
Organism=Escherichia coli, GI1789887, Length=478, Percent_Identity=23.8493723849372, Blast_Score=119, Evalue=7e-28,
Organism=Escherichia coli, GI1787551, Length=472, Percent_Identity=25.8474576271186, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI87081878, Length=503, Percent_Identity=23.2604373757455, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 63774; Mature: 63774

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKINFEWVNSMLDVFLPIRILLLLTVFMGFFSPLAAVASVTGADAAKKENFETPVDGGR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCE
ILFGSIGEASNLIPYLSADSASHEVAGLIYVAPLRYDKDLQVEPWAAESWSMEDEGRLMR
EEEECCCCCCCCCCEEECCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEE
FTLRKGILWEDGVELTAEDVAYTCKVVADPATGSPYAEDFSRIKELRVIDRYTFEVRYEH
EEHHCCCEECCCCCEEHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEHHHHH
FFARAVSSWMQPILPRHLLDGQDIRNSPLTRKPVGAGPYRLKAWEPGSRIILEASPTYFD
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCEEC
GKPYITEAVYRIIPDGATMFMEARAGRLDIMDLSPLQYLRQTSGPMWEREFNKFRYLASA
CCCHHHHHHHHHCCCCHHEEEECCCCEEEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHH
YVFLGFNLQHPFFGDVRVRRAISQAIDRDSIIQGVLLGQGIPAFGPFKPGVWAYHPTLKP
HHEEEECCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCC
IPQNVAAARALLAEAGFTDSDGDGLLDRDGKPFSFTILTNQGNEQRILTATVIQAQLRAI
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEHHHHHHHHHHHHE
GIDVHIRTVEWAAFIREFVNKGRFDAIILGWTIPQDPDIYQVWHSSQAVEGGLNFTYYKN
EEEEEEEEHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHCCCHHHCCCCEEEEECC
PEVDRLLEEARSIPDQSTRARLYCRIQEILDAEQPYCFLYVPYALPVVQKRFQGVKPALA
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCHHHH
GLMYNFEKWWVPKELQRHSLSAD
HHHHHHHHCCCCHHHHHHCCCCC
>Mature Secondary Structure
MKKINFEWVNSMLDVFLPIRILLLLTVFMGFFSPLAAVASVTGADAAKKENFETPVDGGR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCE
ILFGSIGEASNLIPYLSADSASHEVAGLIYVAPLRYDKDLQVEPWAAESWSMEDEGRLMR
EEEECCCCCCCCCCEEECCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEE
FTLRKGILWEDGVELTAEDVAYTCKVVADPATGSPYAEDFSRIKELRVIDRYTFEVRYEH
EEHHCCCEECCCCCEEHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEHHHHH
FFARAVSSWMQPILPRHLLDGQDIRNSPLTRKPVGAGPYRLKAWEPGSRIILEASPTYFD
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCEEC
GKPYITEAVYRIIPDGATMFMEARAGRLDIMDLSPLQYLRQTSGPMWEREFNKFRYLASA
CCCHHHHHHHHHCCCCHHEEEECCCCEEEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHH
YVFLGFNLQHPFFGDVRVRRAISQAIDRDSIIQGVLLGQGIPAFGPFKPGVWAYHPTLKP
HHEEEECCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCC
IPQNVAAARALLAEAGFTDSDGDGLLDRDGKPFSFTILTNQGNEQRILTATVIQAQLRAI
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEHHHHHHHHHHHHE
GIDVHIRTVEWAAFIREFVNKGRFDAIILGWTIPQDPDIYQVWHSSQAVEGGLNFTYYKN
EEEEEEEEHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHCCCHHHCCCCEEEEECC
PEVDRLLEEARSIPDQSTRARLYCRIQEILDAEQPYCFLYVPYALPVVQKRFQGVKPALA
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCHHHH
GLMYNFEKWWVPKELQRHSLSAD
HHHHHHHHCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]