Click here to switch to the map view.

The map label for this gene is malK [H]

Identifier: 220904803

GI number: 220904803

Start: 1833121

End: 1833747

Strand: Reverse

Name: malK [H]

Synonym: Ddes_1535

Alternate gene names: 220904803

Gene position: 1833747-1833121 (Counterclockwise)

Preceding gene: 220904804

Following gene: 220904794

Centisome position: 63.82

GC content: 52.63

Gene sequence:

>627_bases
ATGGATTTCATTAAAAATAACAGCCTGTTCATCAAGCTGGTGATTATGGTCAGTCTGGCCCTCTGCCCACCCATCCTGTT
GAGTCATCTGGCCGGATCGTATTTTATCAGCAAGTATGGTTTTGAGCAGGCCGAGCAGACAGTGGCCAATGTGGCCCAGC
TCACGGCGGAATCCTCGGCGGTTATTGAGGGCATGGAGGAGCCGCGTGGGGAGGCCTGGAAGCAGATGGCGGATTTTCTC
GGCATGTTGACCAGTGTTTCCGGGGTCAAATTTATTGTGCTGATAGATATGAACGGCAACCGGATATACCATCCCGAGGC
ACAGAAAATCGGCGCTCATGTGGTTGGGGGCGATGAAGGCGAGGCCCTGAAAGGTAAAACATATATTTCTTCGGCCCGTG
GTACCTTCGGGTTTTCACAACGGGCGTTTCGGCCGGTATATGCAGAAAACGGACGGCAGGTGGGCGTTGTTGTTGTGGGC
ATCATGTCGCGCGACGAGGTACGTTCACCTGCAAATATTTGTGGTGCGGCTTTTCCAGAAAACCTGCGCAGACAGCGGCC
TTTTGCAATGTTTTCATTGCCGTCATCCTGTCCCGTATTTGAGCCATATGCGCAATGGTGCAGGTAA

Upstream 100 bases:

>100_bases
CGGCCTTCGAGCAGGCTCGGCCAGTCCGTGTGCATCTGCGGCATGAAGCGCCGTCAGCAGCCATTTCAGAACCAGCACCC
AAGCAACGGCAGATATTCTG

Downstream 100 bases:

>100_bases
GGGGCAGGGCTGTACTCTTCGAAGCCTTCTTTGTATTCCCCGGGGCCATCTCCGGACATCCCATTAGTATTTTAAAGCGT
CGGCAATGCATCGGCAGGTT

Product: signal transduction histidine kinase regulating citrate/malate metabolism

Products: NA

Alternate protein names: Malate kinase sensor [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MDFIKNNSLFIKLVIMVSLALCPPILLSHLAGSYFISKYGFEQAEQTVANVAQLTAESSAVIEGMEEPRGEAWKQMADFL
GMLTSVSGVKFIVLIDMNGNRIYHPEAQKIGAHVVGGDEGEALKGKTYISSARGTFGFSQRAFRPVYAENGRQVGVVVVG
IMSRDEVRSPANICGAAFPENLRRQRPFAMFSLPSSCPVFEPYAQWCR

Sequences:

>Translated_208_residues
MDFIKNNSLFIKLVIMVSLALCPPILLSHLAGSYFISKYGFEQAEQTVANVAQLTAESSAVIEGMEEPRGEAWKQMADFL
GMLTSVSGVKFIVLIDMNGNRIYHPEAQKIGAHVVGGDEGEALKGKTYISSARGTFGFSQRAFRPVYAENGRQVGVVVVG
IMSRDEVRSPANICGAAFPENLRRQRPFAMFSLPSSCPVFEPYAQWCR
>Mature_208_residues
MDFIKNNSLFIKLVIMVSLALCPPILLSHLAGSYFISKYGFEQAEQTVANVAQLTAESSAVIEGMEEPRGEAWKQMADFL
GMLTSVSGVKFIVLIDMNGNRIYHPEAQKIGAHVVGGDEGEALKGKTYISSARGTFGFSQRAFRPVYAENGRQVGVVVVG
IMSRDEVRSPANICGAAFPENLRRQRPFAMFSLPSSCPVFEPYAQWCR

Specific function: Member of a two-component regulatory system malK/malR. Involved in the activation of maeA, maeN and yflS in presence of malate. Probably activates malR by phosphorylation [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790567, Length=158, Percent_Identity=31.6455696202532, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1786837, Length=154, Percent_Identity=25.3246753246753, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 22848; Mature: 22848

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFIKNNSLFIKLVIMVSLALCPPILLSHLAGSYFISKYGFEQAEQTVANVAQLTAESSA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
VIEGMEEPRGEAWKQMADFLGMLTSVSGVKFIVLIDMNGNRIYHPEAQKIGAHVVGGDEG
HHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEECCCHHHCCCEEECCCCC
EALKGKTYISSARGTFGFSQRAFRPVYAENGRQVGVVVVGIMSRDEVRSPANICGAAFPE
CCCCCHHHHHCCCCCCCCCHHHCCCHHHCCCCEEEEEEEEECCHHHHCCCHHHHHHCCCH
NLRRQRPFAMFSLPSSCPVFEPYAQWCR
HHHHCCCEEEEECCCCCCCCCHHHHHCC
>Mature Secondary Structure
MDFIKNNSLFIKLVIMVSLALCPPILLSHLAGSYFISKYGFEQAEQTVANVAQLTAESSA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
VIEGMEEPRGEAWKQMADFLGMLTSVSGVKFIVLIDMNGNRIYHPEAQKIGAHVVGGDEG
HHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEECCCHHHCCCEEECCCCC
EALKGKTYISSARGTFGFSQRAFRPVYAENGRQVGVVVVGIMSRDEVRSPANICGAAFPE
CCCCCHHHHHCCCCCCCCCHHHCCCHHHCCCCEEEEEEEEECCHHHHCCCHHHHHHCCCH
NLRRQRPFAMFSLPSSCPVFEPYAQWCR
HHHHCCCEEEEECCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 12949159; 12949160 [H]