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The map label for this gene is mnmE [H]

Identifier: 220904769

GI number: 220904769

Start: 1792451

End: 1793677

Strand: Reverse

Name: mnmE [H]

Synonym: Ddes_1501

Alternate gene names: 220904769

Gene position: 1793677-1792451 (Counterclockwise)

Preceding gene: 220904770

Following gene: 220904766

Centisome position: 62.42

GC content: 61.61

Gene sequence:

>1227_bases
ATGAAAGATGCGCCCAAGGGGCTTCGTCTGCATATCGCCGTTCTCGGTCGCTGCAATGTGGGCAAGTCTTCGCTGCTCAA
CGCATTGTGTGGGCAGGACGTTGCCATTGTGTCCGCCACTCCGGGCACGACAGCCGACCCGGTGGAAAAAACGCTTGAAA
TGGCGCCGCTGGGGCCGGTTGTTTTTCTTGATACCGCCGGTACTGACGATACGGGCGAGCTTGGAGGATTGCGCGCCGGG
CGGAGCCTTGCGGCCATGACCAGAGCTGACCTGGCTTTGCTGGTAACGGATTCTCCGTCCTGGGGGCCGTATGAACGTGA
CCTTGCCGCTCGCCTGAAAGAGCAGGAAATACCCTTTGTGGCCGTGCGCAACAAGGCGGACCAGCCGGGGAGCATGGCGC
GCCCACAAGGCCTGTCCGATGAAGTGCCTTTTGTCTGCGTTTCGGCGTACAGCGGCCAGGGACTGGATGCTGTACGCGCG
GCCCTGATGGCGGCAGCCCCTGAGGGTGCTCTGCGGCAGCCGCCGCTGGCAAGCGATCTGCTGCCTGACAAGGGGGTGCT
TGTCCTCGTGACGCCCATTGACTCCGGCGCCCCCCGCGGCAGGCTGATTCTGCCGCAGGTTCAGACCATTCGTGACAGCC
TGGACGGGCGAAAGATATGCCTTGTTGTCACAGAGGCGGAATTGCCCGCAGCGCTGCGCTGTCTGAAAAAAGCGCCCGAT
CTGGTCGTGTGTGATTCGCAGGTGGTGCATGCGGTCGATCGTCATACACCGGAGTATATTCCCATGACGACCTTCTCCAT
TCTCATGGCCCGGTTCAAAGGTGATCTGACCTGTCTGGTCCAAGGTGCGGCCACGCTGAGAAAACTCAGGTCCGGTGACA
GTGTGCTGATTCAGGAGGCGTGCTCCCATCATGCACAGAAGGATGATATCGGCAGGGTCAAACTGCCGCGCCTGCTGCAA
AAAATGGCGGGTGGCGGCTTGCGTATTTCCATTGCCGCAGGCAAGGAGTTTTCCGGGTACAGCGGGGATTGCAAGGCCGT
CGTGCATTGCGGCGGCTGCGTTATTACGCGCGGGCAGATGATGGCGCGCCTGCACGCTGCAACCCGGGCGGGGCTGCCCA
TCACCAATTACGGAGTGGCTATTTCTCTCGCCCAAGGGGTGCTGCCGCGTGTACTTGCGCCTTTTCCCGAGGCGCTGCGG
GCGTATGAAGACGCGTGCTGCCTGTGA

Upstream 100 bases:

>100_bases
GGAACCTGCACCCGGGTCCCCCGCCCGAACAGGCCGGCACGGTTTACCCCGCTGTGCCGGCCGCCACGTTCCGGCGGAAA
CACCTGTATGGGAGTTTGTC

Downstream 100 bases:

>100_bases
CCGGTATGCGTGCGGTTACTAGGTGTCGGACGCATGCGTGTCCCGAAACGTCAAGGCCCCGGCATATACGCCCGGGGCCT
TGACGGTGAAAGATCGGGAA

Product: small GTP-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MKDAPKGLRLHIAVLGRCNVGKSSLLNALCGQDVAIVSATPGTTADPVEKTLEMAPLGPVVFLDTAGTDDTGELGGLRAG
RSLAAMTRADLALLVTDSPSWGPYERDLAARLKEQEIPFVAVRNKADQPGSMARPQGLSDEVPFVCVSAYSGQGLDAVRA
ALMAAAPEGALRQPPLASDLLPDKGVLVLVTPIDSGAPRGRLILPQVQTIRDSLDGRKICLVVTEAELPAALRCLKKAPD
LVVCDSQVVHAVDRHTPEYIPMTTFSILMARFKGDLTCLVQGAATLRKLRSGDSVLIQEACSHHAQKDDIGRVKLPRLLQ
KMAGGGLRISIAAGKEFSGYSGDCKAVVHCGGCVITRGQMMARLHAATRAGLPITNYGVAISLAQGVLPRVLAPFPEALR
AYEDACCL

Sequences:

>Translated_408_residues
MKDAPKGLRLHIAVLGRCNVGKSSLLNALCGQDVAIVSATPGTTADPVEKTLEMAPLGPVVFLDTAGTDDTGELGGLRAG
RSLAAMTRADLALLVTDSPSWGPYERDLAARLKEQEIPFVAVRNKADQPGSMARPQGLSDEVPFVCVSAYSGQGLDAVRA
ALMAAAPEGALRQPPLASDLLPDKGVLVLVTPIDSGAPRGRLILPQVQTIRDSLDGRKICLVVTEAELPAALRCLKKAPD
LVVCDSQVVHAVDRHTPEYIPMTTFSILMARFKGDLTCLVQGAATLRKLRSGDSVLIQEACSHHAQKDDIGRVKLPRLLQ
KMAGGGLRISIAAGKEFSGYSGDCKAVVHCGGCVITRGQMMARLHAATRAGLPITNYGVAISLAQGVLPRVLAPFPEALR
AYEDACCL
>Mature_408_residues
MKDAPKGLRLHIAVLGRCNVGKSSLLNALCGQDVAIVSATPGTTADPVEKTLEMAPLGPVVFLDTAGTDDTGELGGLRAG
RSLAAMTRADLALLVTDSPSWGPYERDLAARLKEQEIPFVAVRNKADQPGSMARPQGLSDEVPFVCVSAYSGQGLDAVRA
ALMAAAPEGALRQPPLASDLLPDKGVLVLVTPIDSGAPRGRLILPQVQTIRDSLDGRKICLVVTEAELPAALRCLKKAPD
LVVCDSQVVHAVDRHTPEYIPMTTFSILMARFKGDLTCLVQGAATLRKLRSGDSVLIQEACSHHAQKDDIGRVKLPRLLQ
KMAGGGLRISIAAGKEFSGYSGDCKAVVHCGGCVITRGQMMARLHAATRAGLPITNYGVAISLAQGVLPRVLAPFPEALR
AYEDACCL

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG1160

COG function: function code R; Predicted GTPases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Escherichia coli, GI2367268, Length=169, Percent_Identity=31.9526627218935, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 43006; Mature: 43006

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDAPKGLRLHIAVLGRCNVGKSSLLNALCGQDVAIVSATPGTTADPVEKTLEMAPLGPV
CCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCCE
VFLDTAGTDDTGELGGLRAGRSLAAMTRADLALLVTDSPSWGPYERDLAARLKEQEIPFV
EEEECCCCCCCCCCCCCCCCCHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEE
AVRNKADQPGSMARPQGLSDEVPFVCVSAYSGQGLDAVRAALMAAAPEGALRQPPLASDL
EECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHC
LPDKGVLVLVTPIDSGAPRGRLILPQVQTIRDSLDGRKICLVVTEAELPAALRCLKKAPD
CCCCCEEEEEEECCCCCCCCCEECCHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCC
LVVCDSQVVHAVDRHTPEYIPMTTFSILMARFKGDLTCLVQGAATLRKLRSGDSVLIQEA
EEEECHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCHHHHHHH
CSHHAQKDDIGRVKLPRLLQKMAGGGLRISIAAGKEFSGYSGDCKAVVHCGGCVITRGQM
HHHCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEEEECCEEEEHHHH
MARLHAATRAGLPITNYGVAISLAQGVLPRVLAPFPEALRAYEDACCL
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKDAPKGLRLHIAVLGRCNVGKSSLLNALCGQDVAIVSATPGTTADPVEKTLEMAPLGPV
CCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCCE
VFLDTAGTDDTGELGGLRAGRSLAAMTRADLALLVTDSPSWGPYERDLAARLKEQEIPFV
EEEECCCCCCCCCCCCCCCCCHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEE
AVRNKADQPGSMARPQGLSDEVPFVCVSAYSGQGLDAVRAALMAAAPEGALRQPPLASDL
EECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHC
LPDKGVLVLVTPIDSGAPRGRLILPQVQTIRDSLDGRKICLVVTEAELPAALRCLKKAPD
CCCCCEEEEEEECCCCCCCCCEECCHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCC
LVVCDSQVVHAVDRHTPEYIPMTTFSILMARFKGDLTCLVQGAATLRKLRSGDSVLIQEA
EEEECHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCHHHHHHH
CSHHAQKDDIGRVKLPRLLQKMAGGGLRISIAAGKEFSGYSGDCKAVVHCGGCVITRGQM
HHHCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEEEECCEEEEHHHH
MARLHAATRAGLPITNYGVAISLAQGVLPRVLAPFPEALRAYEDACCL
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]