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The map label for this gene is metH [H]

Identifier: 220904710

GI number: 220904710

Start: 1726873

End: 1729281

Strand: Reverse

Name: metH [H]

Synonym: Ddes_1442

Alternate gene names: 220904710

Gene position: 1729281-1726873 (Counterclockwise)

Preceding gene: 220904726

Following gene: 220904709

Centisome position: 60.18

GC content: 60.32

Gene sequence:

>2409_bases
ATGACTTTCAGACAAGCGCTCGGTTCGGGACGTCCTCTTTTGCTTGACGGCGCCATGGGCACCATGCTTCAGGCTTCCGG
TCTTCCTGCGGGTACAAGCCCGGAAGAATTTTGCATGGAAAATCCCGATATTCTGCGGGGTATCCATGCGTCCTACCTCA
AGGCGGGCGTTGATCTGCTTACGTCTTGCACCTTCGGCGGCAATGCTTACAAGCTTTCGAAAAATCTTGATGTTTTTTCT
TTTAACAAGCGTATGGCCTCGGTGGCGCGCGCGGCTGCGGCCGATGTGTCACGCCCTGACGGCCGCCCGATTTTCGTGGC
GGGCAACGTGGGACCCACCGGACATTTCGCCAAACCTTTGGGTCCTGTTGAACCTGCCGAGCTTATTGAAGTTTTCGCCC
AGCAGATACGAGGGCTTGTGGCCGGCGGGGCTGATCTTGTTTTCATCGAAACCCAGTTTGACCTGGCCGAGGCCAGGGCC
GCCGTGGTGGCCGCCAGACAGGTCTGTGACCTGCCGGTAATGGTTTCTATGACTTTTGAGCAGGGCGTAAGCCTTACGGG
ATCTACCCCCGCCATATTTGCCGAAACCATGCAAAACATGGGCGTGGATGTGGTGGGAACCAACTGCAGCCTCGGGCCGG
AACAGATGGAGCCTGTGGTTGCCGAACTTCTGGGCGTGTGCGCCTGCCCGGTCATGGCCGAGCCGAATGCCGGCCTGCCC
GAGCTGCGGGGAACGCAGACGGTATTCCCGCTGGGGCCGGAGGACTTCGCCCGCAAGACAGCTTCTTTTGCCCATATGGG
CGCGCAGGTGCTCGGCGGCTGTTGCGGTACCACACCCGAACATCTTGCGGCTCTGGCGCACAGTCTGCGCGGCCTTGGCG
AGGTGCGCCCCACGGGTGTGAAGCGCAGCGGCATCTGCCTGACGAGCCGTTCGCAGATGGTGCGCATCGGCGCGGGACGG
CCTCTTGCCATTATCGGCGAACGTATCAATCCCACAGGCAAAAAGCAGCTTACTCTTGAACTTCAGGAAGGGCGCTTTGA
CACGGCCCTGCAATTTGCTGATGCGCAGATTGAGGCCGGAGCCACTGTACTGGACGTGAATGTGGGCGCGCCGCTTGTGG
ATGAAACCGCGCTTTTGCCCGACCTGGTGCAGCGTCTGGTGGGGCGGCTCACGGTGCCGCTTTCTCTGGACTCATCCAAT
GCCCAGGCCATTGCCGCCGCCCTGCCGTACTGCCCCGGCTCTTTTCTGGTGAATTCCATCAGTGGCGAAGAAGGCCGGAT
GGAACTGCTGGGGCCTCTATGCCGCGATTACGGCGCGCCTTTCATCCTGCTGCCGCTCGAGGGCGCAACCTTGCCCGAAA
AGGCCGTGGAACGGATCCGCACTGTGGAGAGCCTTCTTCTGCGGGCGGAACGTCTCGGCATTCCACGGCGGCTCATGATG
GTGGATATTCTGGCTCTGGCAGTCTCCTCCAGTCCGGACGGCGCGCGCCAGTGCCTTGAAATGACGCGCTGGTGTGCAGA
GCAGGGCCTGCCGACAACCCTGGGGCTTTCCAATCTTTCTTTCGGCCTGCCCGCGCGCGACCTGCTTAATGCCACATTTC
TGGCCTACGCCGCCGGAGCGGGACTTTCATCGTGCATTGCCAATCCATCAGCCCAGCGCCTGCGCGAGGCGGCTGATGCA
CTCAAGGTGCTGGGCGAGCATGATCCCCATGCGTCTTCATTCATTTCAAGTTACGCCAACTGGAAGCCCGGCGAAGGCAG
CGTACTGCAACGCCAGGGGGGCGGTGGGGCGTCCAAAAGCCTTGGCGAGGCCGTACTCAACGGCGACAGGGAAAATGTCC
TGTCCCTGCTTGAAGCCGAACTGGCCGCAGGGGCCGAGCCTTTCAGCCTTGTGCAGGAAGTGCTTATTCCGGCTATTACC
GAGGTGGGCGCACGCTACGAACGGCGCGAATATTTTTTGCCCCAGCTTATCCGCGCGGCCGAAACCATGCAGACGGCTTT
TGGTCATCTGAAGCCTCGCCTCGAGGCCGGGCGGGGGGCCGAAGAGCGCCCTGTGGTGATTATGGCCACGGTGGAAGGGG
ATATTCATGATATTGGCAAAAATATTGTTTCCCTGCTTTTGGGAAACCACGGTTTTGATGTGATTGACGCGGGCAAGGAT
GTGCCTGCCGAAGATATCGTGGCTTGCGCCCTTGAGCATAACGCGCGTATTATCGGCCTGTCGGCCCTGATGACCACGAC
AATGGTGCGTATGGAAGACACCATAAAACTGGTGAAGGAAAAAGGGCTGGCTATCAAGGTGATGGTGGGGGGCGCGGCTG
TTACCCAGGCTTTTGCCGATGCGATCGGGGCCGATGCCTACTGTGCGGATGCCGTGGGCGCTGTGCGGGCCGCCAAGAAT
TTTATGTAG

Upstream 100 bases:

>100_bases
TATACAGGTATTATGGCTGGAAAGCAAAGACGGGCTTTGCGCGGCAGTGGGGAAGGGCCTGTTTTTTCAATAAACTATGA
CGGCCAAGGTGGCGGCATTC

Downstream 100 bases:

>100_bases
CATTGTTCTGCATTACGCGGGTGCGCTCATGCCGGGCGCCCGCGCGCAAGGCCCGGCGGCATGGTAACCCTTCGCATATT
CCGGCCTTCCTAATGGCCTG

Product: homocysteine S-methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 802; Mature: 801

Protein sequence:

>802_residues
MTFRQALGSGRPLLLDGAMGTMLQASGLPAGTSPEEFCMENPDILRGIHASYLKAGVDLLTSCTFGGNAYKLSKNLDVFS
FNKRMASVARAAAADVSRPDGRPIFVAGNVGPTGHFAKPLGPVEPAELIEVFAQQIRGLVAGGADLVFIETQFDLAEARA
AVVAARQVCDLPVMVSMTFEQGVSLTGSTPAIFAETMQNMGVDVVGTNCSLGPEQMEPVVAELLGVCACPVMAEPNAGLP
ELRGTQTVFPLGPEDFARKTASFAHMGAQVLGGCCGTTPEHLAALAHSLRGLGEVRPTGVKRSGICLTSRSQMVRIGAGR
PLAIIGERINPTGKKQLTLELQEGRFDTALQFADAQIEAGATVLDVNVGAPLVDETALLPDLVQRLVGRLTVPLSLDSSN
AQAIAAALPYCPGSFLVNSISGEEGRMELLGPLCRDYGAPFILLPLEGATLPEKAVERIRTVESLLLRAERLGIPRRLMM
VDILALAVSSSPDGARQCLEMTRWCAEQGLPTTLGLSNLSFGLPARDLLNATFLAYAAGAGLSSCIANPSAQRLREAADA
LKVLGEHDPHASSFISSYANWKPGEGSVLQRQGGGGASKSLGEAVLNGDRENVLSLLEAELAAGAEPFSLVQEVLIPAIT
EVGARYERREYFLPQLIRAAETMQTAFGHLKPRLEAGRGAEERPVVIMATVEGDIHDIGKNIVSLLLGNHGFDVIDAGKD
VPAEDIVACALEHNARIIGLSALMTTTMVRMEDTIKLVKEKGLAIKVMVGGAAVTQAFADAIGADAYCADAVGAVRAAKN
FM

Sequences:

>Translated_802_residues
MTFRQALGSGRPLLLDGAMGTMLQASGLPAGTSPEEFCMENPDILRGIHASYLKAGVDLLTSCTFGGNAYKLSKNLDVFS
FNKRMASVARAAAADVSRPDGRPIFVAGNVGPTGHFAKPLGPVEPAELIEVFAQQIRGLVAGGADLVFIETQFDLAEARA
AVVAARQVCDLPVMVSMTFEQGVSLTGSTPAIFAETMQNMGVDVVGTNCSLGPEQMEPVVAELLGVCACPVMAEPNAGLP
ELRGTQTVFPLGPEDFARKTASFAHMGAQVLGGCCGTTPEHLAALAHSLRGLGEVRPTGVKRSGICLTSRSQMVRIGAGR
PLAIIGERINPTGKKQLTLELQEGRFDTALQFADAQIEAGATVLDVNVGAPLVDETALLPDLVQRLVGRLTVPLSLDSSN
AQAIAAALPYCPGSFLVNSISGEEGRMELLGPLCRDYGAPFILLPLEGATLPEKAVERIRTVESLLLRAERLGIPRRLMM
VDILALAVSSSPDGARQCLEMTRWCAEQGLPTTLGLSNLSFGLPARDLLNATFLAYAAGAGLSSCIANPSAQRLREAADA
LKVLGEHDPHASSFISSYANWKPGEGSVLQRQGGGGASKSLGEAVLNGDRENVLSLLEAELAAGAEPFSLVQEVLIPAIT
EVGARYERREYFLPQLIRAAETMQTAFGHLKPRLEAGRGAEERPVVIMATVEGDIHDIGKNIVSLLLGNHGFDVIDAGKD
VPAEDIVACALEHNARIIGLSALMTTTMVRMEDTIKLVKEKGLAIKVMVGGAAVTQAFADAIGADAYCADAVGAVRAAKN
FM
>Mature_801_residues
TFRQALGSGRPLLLDGAMGTMLQASGLPAGTSPEEFCMENPDILRGIHASYLKAGVDLLTSCTFGGNAYKLSKNLDVFSF
NKRMASVARAAAADVSRPDGRPIFVAGNVGPTGHFAKPLGPVEPAELIEVFAQQIRGLVAGGADLVFIETQFDLAEARAA
VVAARQVCDLPVMVSMTFEQGVSLTGSTPAIFAETMQNMGVDVVGTNCSLGPEQMEPVVAELLGVCACPVMAEPNAGLPE
LRGTQTVFPLGPEDFARKTASFAHMGAQVLGGCCGTTPEHLAALAHSLRGLGEVRPTGVKRSGICLTSRSQMVRIGAGRP
LAIIGERINPTGKKQLTLELQEGRFDTALQFADAQIEAGATVLDVNVGAPLVDETALLPDLVQRLVGRLTVPLSLDSSNA
QAIAAALPYCPGSFLVNSISGEEGRMELLGPLCRDYGAPFILLPLEGATLPEKAVERIRTVESLLLRAERLGIPRRLMMV
DILALAVSSSPDGARQCLEMTRWCAEQGLPTTLGLSNLSFGLPARDLLNATFLAYAAGAGLSSCIANPSAQRLREAADAL
KVLGEHDPHASSFISSYANWKPGEGSVLQRQGGGGASKSLGEAVLNGDRENVLSLLEAELAAGAEPFSLVQEVLIPAITE
VGARYERREYFLPQLIRAAETMQTAFGHLKPRLEAGRGAEERPVVIMATVEGDIHDIGKNIVSLLLGNHGFDVIDAGKDV
PAEDIVACALEHNARIIGLSALMTTTMVRMEDTIKLVKEKGLAIKVMVGGAAVTQAFADAIGADAYCADAVGAVRAAKNF
M

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=871, Percent_Identity=29.8507462686567, Blast_Score=312, Evalue=1e-84,
Organism=Homo sapiens, GI13162290, Length=313, Percent_Identity=26.1980830670927, Blast_Score=78, Evalue=4e-14,
Organism=Homo sapiens, GI157266337, Length=385, Percent_Identity=24.6753246753247, Blast_Score=75, Evalue=3e-13,
Organism=Escherichia coli, GI1790450, Length=844, Percent_Identity=29.1469194312796, Blast_Score=310, Evalue=3e-85,
Organism=Escherichia coli, GI1786456, Length=295, Percent_Identity=26.7796610169492, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17535405, Length=848, Percent_Identity=29.8349056603774, Blast_Score=300, Evalue=2e-81,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 84335; Mature: 84204

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50970 HCY ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFRQALGSGRPLLLDGAMGTMLQASGLPAGTSPEEFCMENPDILRGIHASYLKAGVDLL
CCCHHHHCCCCCEEEECCHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHH
TSCTFGGNAYKLSKNLDVFSFNKRMASVARAAAADVSRPDGRPIFVAGNVGPTGHFAKPL
HHCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCC
GPVEPAELIEVFAQQIRGLVAGGADLVFIETQFDLAEARAAVVAARQVCDLPVMVSMTFE
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEEEHH
QGVSLTGSTPAIFAETMQNMGVDVVGTNCSLGPEQMEPVVAELLGVCACPVMAEPNAGLP
CCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
ELRGTQTVFPLGPEDFARKTASFAHMGAQVLGGCCGTTPEHLAALAHSLRGLGEVRPTGV
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCC
KRSGICLTSRSQMVRIGAGRPLAIIGERINPTGKKQLTLELQEGRFDTALQFADAQIEAG
CCCCEEEECCCCEEEECCCCCEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHCCHHHCC
ATVLDVNVGAPLVDETALLPDLVQRLVGRLTVPLSLDSSNAQAIAAALPYCPGSFLVNSI
CEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHCCCCCHHHHHCCC
SGEEGRMELLGPLCRDYGAPFILLPLEGATLPEKAVERIRTVESLLLRAERLGIPRRLMM
CCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VDILALAVSSSPDGARQCLEMTRWCAEQGLPTTLGLSNLSFGLPARDLLNATFLAYAAGA
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCC
GLSSCIANPSAQRLREAADALKVLGEHDPHASSFISSYANWKPGEGSVLQRQGGGGASKS
CHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCHHH
LGEAVLNGDRENVLSLLEAELAAGAEPFSLVQEVLIPAITEVGARYERREYFLPQLIRAA
HHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETMQTAFGHLKPRLEAGRGAEERPVVIMATVEGDIHDIGKNIVSLLLGNHGFDVIDAGKD
HHHHHHHHHCCCHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEECCCC
VPAEDIVACALEHNARIIGLSALMTTTMVRMEDTIKLVKEKGLAIKVMVGGAAVTQAFAD
CCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHH
AIGADAYCADAVGAVRAAKNFM
HHCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TFRQALGSGRPLLLDGAMGTMLQASGLPAGTSPEEFCMENPDILRGIHASYLKAGVDLL
CCHHHHCCCCCEEEECCHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHH
TSCTFGGNAYKLSKNLDVFSFNKRMASVARAAAADVSRPDGRPIFVAGNVGPTGHFAKPL
HHCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCC
GPVEPAELIEVFAQQIRGLVAGGADLVFIETQFDLAEARAAVVAARQVCDLPVMVSMTFE
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEEEHH
QGVSLTGSTPAIFAETMQNMGVDVVGTNCSLGPEQMEPVVAELLGVCACPVMAEPNAGLP
CCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
ELRGTQTVFPLGPEDFARKTASFAHMGAQVLGGCCGTTPEHLAALAHSLRGLGEVRPTGV
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCC
KRSGICLTSRSQMVRIGAGRPLAIIGERINPTGKKQLTLELQEGRFDTALQFADAQIEAG
CCCCEEEECCCCEEEECCCCCEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHCCHHHCC
ATVLDVNVGAPLVDETALLPDLVQRLVGRLTVPLSLDSSNAQAIAAALPYCPGSFLVNSI
CEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHCCCCCHHHHHCCC
SGEEGRMELLGPLCRDYGAPFILLPLEGATLPEKAVERIRTVESLLLRAERLGIPRRLMM
CCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VDILALAVSSSPDGARQCLEMTRWCAEQGLPTTLGLSNLSFGLPARDLLNATFLAYAAGA
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCC
GLSSCIANPSAQRLREAADALKVLGEHDPHASSFISSYANWKPGEGSVLQRQGGGGASKS
CHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCHHH
LGEAVLNGDRENVLSLLEAELAAGAEPFSLVQEVLIPAITEVGARYERREYFLPQLIRAA
HHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETMQTAFGHLKPRLEAGRGAEERPVVIMATVEGDIHDIGKNIVSLLLGNHGFDVIDAGKD
HHHHHHHHHCCCHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEECCCC
VPAEDIVACALEHNARIIGLSALMTTTMVRMEDTIKLVKEKGLAIKVMVGGAAVTQAFAD
CCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHH
AIGADAYCADAVGAVRAAKNFM
HHCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]