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The map label for this gene is pfkA [H]

Identifier: 220904691

GI number: 220904691

Start: 1702022

End: 1703329

Strand: Direct

Name: pfkA [H]

Synonym: Ddes_1423

Alternate gene names: 220904691

Gene position: 1702022-1703329 (Clockwise)

Preceding gene: 220904690

Following gene: 220904695

Centisome position: 59.23

GC content: 57.8

Gene sequence:

>1308_bases
ATGAATAAAGAATGTACTCTGCAAACGGATATACGCACCCTTGGTCCCTGCAAGCTCGATTCCCACCTGCCCTATCGCAC
CTGGGCCGATGACAGGACCGTACAGATTTTTGTGGATGAAGATCTGACAGACGACGCCGGCAAGGCCTTTAGCGTATGCC
TCGAGGCCGCCGGGCCACGCAAAAAGCTCTACTTTGACTCCACGCGGGCCAAATGCGCCATCGTGACCTGCGGCGGCCTT
TGCCCCGGCATCAACGATGTCATACGGGCCATCGTAATGGAGGCCTTTCATGCGTACAACGTGCCGTCCATCCTGGGTAT
TCCTTACGGTCTTGAAGGCTTTATCCCCAAGTACGGTCACACCCTCCAGGAACTGACGCCCAAAGGCGTGGCTGACATCC
ATCGCTTTGGCGGAACCATGCTCGGGTCTTCACGCGGGCCGCAGTCCGCCGAGGAAATTGTGGACGCCCTTGAACGCAGC
AATGTCAATATTCTTTTTGTGATCGGCGGCGACGGGACTATGAAGGCCGCGCGGGCCATCAGTACAGAAATTCAGGCCCG
CAACCTGAAACTTGCCGTTATCGGCATTCCCAAAACCATTGATAATGACATCAACTTCATTCCCCAGTCCTTCGGCTTTG
AAACCGCTGTCTACAAGGCCACAGAAGCCATTGAATGCGCCCACACCGAAGCGTGCGGCGTTCCCAACGGCATTGGGCTG
GTGAAGCTTATGGGGCGCGAATCGGGTTTTATCGCCGCCCGGGCCGCGCTGGCGCTCAAGGAAGTAAACTTTGTGCTCAT
TCCCGAAGCGCCATTCAGCCTTGAAGGTGAAGGGGGGCTTCTGCCTGCTCTGGAAGAACGCCTGAAAGCGCGGGGACATG
CCGTCATCGTTGCCGCCGAAGGGGCCGGGCAACATTTGCTTGCGGAACAGAACGGCACCGATGCCTCCGGCAATCCCGTG
CTCGGCGACATCTCCGAACTCCTGCGCACGTCCATCAACACCTATCTGGACACACGTGGCGTGGCTCACTCCATAAAATA
TATCGACCCGAGCTATATCATCCGCTCCATTCCCGCCAGCGCCAACGACAAGGTTTACTGCGGCTTTCTGGGCCAGTACG
CGGTGCACGCCGCCATGGCGGGTCGCACACATATGGTGGTGGGGAAAATTCAGGACCGCTACGTGCACCTGCCGCTGGAA
CTGGTCACGCGCAAGCGCCGCAAACTCAATATCTATTCCGACCTGTGGCGGGCCGTACTTGAATCCACGGGGCAAGGCAT
GCTCAAGGGCATGCTGCCACCGATATAG

Upstream 100 bases:

>100_bases
GCCGGACTTGAGCTGGACGAATTTTTGGCCCTGTCCATCGAGGCCATGCGCGCCAGCGCCCAAGAACTTGGACTGAGCAA
ATAACATGCGCGAGGCCGGT

Downstream 100 bases:

>100_bases
TACAGGCTTGCTTTAGCTGAGCGCCTGCTCCTACAGCCGTCAGAGCAGTTTAAAATTAAATTGCTCTAAAGGCAGCCTTG
TCTGCCAGATCGCTTTTTAC

Product: diphosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MNKECTLQTDIRTLGPCKLDSHLPYRTWADDRTVQIFVDEDLTDDAGKAFSVCLEAAGPRKKLYFDSTRAKCAIVTCGGL
CPGINDVIRAIVMEAFHAYNVPSILGIPYGLEGFIPKYGHTLQELTPKGVADIHRFGGTMLGSSRGPQSAEEIVDALERS
NVNILFVIGGDGTMKAARAISTEIQARNLKLAVIGIPKTIDNDINFIPQSFGFETAVYKATEAIECAHTEACGVPNGIGL
VKLMGRESGFIAARAALALKEVNFVLIPEAPFSLEGEGGLLPALEERLKARGHAVIVAAEGAGQHLLAEQNGTDASGNPV
LGDISELLRTSINTYLDTRGVAHSIKYIDPSYIIRSIPASANDKVYCGFLGQYAVHAAMAGRTHMVVGKIQDRYVHLPLE
LVTRKRRKLNIYSDLWRAVLESTGQGMLKGMLPPI

Sequences:

>Translated_435_residues
MNKECTLQTDIRTLGPCKLDSHLPYRTWADDRTVQIFVDEDLTDDAGKAFSVCLEAAGPRKKLYFDSTRAKCAIVTCGGL
CPGINDVIRAIVMEAFHAYNVPSILGIPYGLEGFIPKYGHTLQELTPKGVADIHRFGGTMLGSSRGPQSAEEIVDALERS
NVNILFVIGGDGTMKAARAISTEIQARNLKLAVIGIPKTIDNDINFIPQSFGFETAVYKATEAIECAHTEACGVPNGIGL
VKLMGRESGFIAARAALALKEVNFVLIPEAPFSLEGEGGLLPALEERLKARGHAVIVAAEGAGQHLLAEQNGTDASGNPV
LGDISELLRTSINTYLDTRGVAHSIKYIDPSYIIRSIPASANDKVYCGFLGQYAVHAAMAGRTHMVVGKIQDRYVHLPLE
LVTRKRRKLNIYSDLWRAVLESTGQGMLKGMLPPI
>Mature_435_residues
MNKECTLQTDIRTLGPCKLDSHLPYRTWADDRTVQIFVDEDLTDDAGKAFSVCLEAAGPRKKLYFDSTRAKCAIVTCGGL
CPGINDVIRAIVMEAFHAYNVPSILGIPYGLEGFIPKYGHTLQELTPKGVADIHRFGGTMLGSSRGPQSAEEIVDALERS
NVNILFVIGGDGTMKAARAISTEIQARNLKLAVIGIPKTIDNDINFIPQSFGFETAVYKATEAIECAHTEACGVPNGIGL
VKLMGRESGFIAARAALALKEVNFVLIPEAPFSLEGEGGLLPALEERLKARGHAVIVAAEGAGQHLLAEQNGTDASGNPV
LGDISELLRTSINTYLDTRGVAHSIKYIDPSYIIRSIPASANDKVYCGFLGQYAVHAAMAGRTHMVVGKIQDRYVHLPLE
LVTRKRRKLNIYSDLWRAVLESTGQGMLKGMLPPI

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453619, Length=354, Percent_Identity=24.2937853107345, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI266453768, Length=354, Percent_Identity=24.2937853107345, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI266453748, Length=354, Percent_Identity=24.2937853107345, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI4505749, Length=354, Percent_Identity=24.2937853107345, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI11321601, Length=291, Percent_Identity=27.8350515463918, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI48762920, Length=274, Percent_Identity=27.007299270073, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790350, Length=332, Percent_Identity=27.1084337349398, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17558788, Length=243, Percent_Identity=27.1604938271605, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25147584, Length=264, Percent_Identity=28.4090909090909, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6321679, Length=371, Percent_Identity=24.2587601078167, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323861, Length=261, Percent_Identity=26.0536398467433, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28573326, Length=361, Percent_Identity=26.3157894736842, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652337, Length=361, Percent_Identity=26.0387811634349, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17647809, Length=359, Percent_Identity=26.1838440111421, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 47019; Mature: 47019

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKECTLQTDIRTLGPCKLDSHLPYRTWADDRTVQIFVDEDLTDDAGKAFSVCLEAAGPR
CCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCC
KKLYFDSTRAKCAIVTCGGLCPGINDVIRAIVMEAFHAYNVPSILGIPYGLEGFIPKYGH
CEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCHHCC
TLQELTPKGVADIHRFGGTMLGSSRGPQSAEEIVDALERSNVNILFVIGGDGTMKAARAI
HHHHHCCCCHHHHHHHCCEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHH
STEIQARNLKLAVIGIPKTIDNDINFIPQSFGFETAVYKATEAIECAHTEACGVPNGIGL
HHHHHHCCEEEEEEECCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHCHHHCCCCCCCCE
VKLMGRESGFIAARAALALKEVNFVLIPEAPFSLEGEGGLLPALEERLKARGHAVIVAAE
EEEECCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEC
GAGQHLLAEQNGTDASGNPVLGDISELLRTSINTYLDTRGVAHSIKYIDPSYIIRSIPAS
CCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHEEECCHHHHHHHCCCC
ANDKVYCGFLGQYAVHAAMAGRTHMVVGKIQDRYVHLPLELVTRKRRKLNIYSDLWRAVL
CCCCEEEEEHHHHHHHHHHCCCCEEEEEEECCCEEECCHHHHHHHHHHCCHHHHHHHHHH
ESTGQGMLKGMLPPI
HHCCCHHHHHCCCCC
>Mature Secondary Structure
MNKECTLQTDIRTLGPCKLDSHLPYRTWADDRTVQIFVDEDLTDDAGKAFSVCLEAAGPR
CCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCC
KKLYFDSTRAKCAIVTCGGLCPGINDVIRAIVMEAFHAYNVPSILGIPYGLEGFIPKYGH
CEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCHHCC
TLQELTPKGVADIHRFGGTMLGSSRGPQSAEEIVDALERSNVNILFVIGGDGTMKAARAI
HHHHHCCCCHHHHHHHCCEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHH
STEIQARNLKLAVIGIPKTIDNDINFIPQSFGFETAVYKATEAIECAHTEACGVPNGIGL
HHHHHHCCEEEEEEECCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHCHHHCCCCCCCCE
VKLMGRESGFIAARAALALKEVNFVLIPEAPFSLEGEGGLLPALEERLKARGHAVIVAAE
EEEECCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEC
GAGQHLLAEQNGTDASGNPVLGDISELLRTSINTYLDTRGVAHSIKYIDPSYIIRSIPAS
CCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHEEECCHHHHHHHCCCC
ANDKVYCGFLGQYAVHAAMAGRTHMVVGKIQDRYVHLPLELVTRKRRKLNIYSDLWRAVL
CCCCEEEEEHHHHHHHHHHCCCCEEEEEEECCCEEECCHHHHHHHHHHCCHHHHHHHHHH
ESTGQGMLKGMLPPI
HHCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA