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The map label for this gene is recJ [H]

Identifier: 220904677

GI number: 220904677

Start: 1683643

End: 1685379

Strand: Direct

Name: recJ [H]

Synonym: Ddes_1409

Alternate gene names: 220904677

Gene position: 1683643-1685379 (Clockwise)

Preceding gene: 220904676

Following gene: 220904678

Centisome position: 58.59

GC content: 60.33

Gene sequence:

>1737_bases
GTGAAAAACTGGCTTTTTCGTACGCCGCCCGCAGGCGGTCCCGTCTGTCTGCCCGCTTCCTGGGCAGAAGAGCTTTCCAT
TTCCCCCCTTCTACTGGAGGTGCTCTGGAGGCGTGGCTTTTCGCATAAACAGGCCATCAATGCCTTTCTGGATGCCCGCC
TTGACAGCCTGACCCCGCCGCACCGCTGGCCCCAGATGCCTGAAGCCGCCCAATTGCTGGCAGACGAACTGCTGGCCGGC
AAAAAGCTGGCTGTGTGGGGCGACTACGATGTGGATGGAATAACAGCTTCCACCCTGGTGCTGGACGTACTGGAAAGCCA
CGGCATTGAGGCTCTCTGGCATATTCCTGACCGGAGGACGGAAGGATACGGGCTGAATGTGAAACAGATCGATGCGCTGG
CCGCGCAGGGTTGCCGCATATTGCTCACCGTTGACTGCGGCATTGCCGATGTAGATGCCGTAAACCATGCACGGCGGATC
GGCATGACCGTTGTAGTATCCGACCACCACCTGCCCCAGGACGACCTGCCCCCCGCCCACGCCCTTTGTAATCCCCGGAT
GTGCCCTGAAGAATCCCTGCCTTGCCCCCATCTGGCAGGCGTGGGCGTGGCCTTTTATCTTATGGGAGCCGTCAACGCGG
CCCTGGCTCCGCATACGGGCAAGAAGCACAGAATGGATAAGTGCCTTGACCTTGTAGCTTTGGGAACACTGGCCGATATC
ATGCCCCTGACCAGCGAAAACCGCATTCTGGTGCGTGGCGGCCTTGGTCATCTTGCCGACGCCTCCCGTCCGGGCATAGC
CGCGCTCAAGGTTGCCAGCGGTATGGACGCCAAAACACGGCTCACCAGCGGGCAGGTAAGCTTTCAGCTTGTACCGCGCA
TCAATGCCGCAGGACGCATTGGCGATGGAGGGTTGGCCCTCCGCCTTTTGCGGCAGAAAGACTTTTCTTCCGCCCTGCCC
CTGGCCCAGCAGCTCGACCAGCTCAATACCGAACGCAAACAGGCGGAAAACACCATCCATAGCGAGGCCCGCGCCCAGGT
TCTGGACATGCTCTCCCGCGAACCAAGAGCCAGCCTTGTCCTGTATGGGCAGGACTGGCATCCGGGCATCGTGGGCATTG
TGGCCTCGCGGATTGTGGAAGAATTCTACAGGCCCACCATCATTCTCTGCCAGGATCAGGGCAGCCTGAAAGGGTCCGGC
CGTTCTGTGAGGGAATTTGACTTGCACGCGGGTTTATGCCAGACCGCCAGCAGCCTTTCGGGGTTCGGCGGTCACAGGCT
GGCCGCAGGCGTTCGCCTGCCCCTGGAAAACCTGGCCCGCTTTCGCACGGAATTTGAAAATTTCGTTGCCCAACAGCTTG
GCACAAATCCCCTCAGCCCCAGCCTGACGCTGGAATGCCAACTGGATTTCGCCCAGGCCAGCAACCAGAGTTTTCTTAAA
GAACTGGAGATGCTCCAGCCTTTTGGCCCGGGCAATGCCGAACCGGTCTTTGCCTCGCCGCCGCTTCTGGTCAAGGAGCG
CTCGCCTCTGGGGCGCGGCGGCGAACACGTGCGTCTGCGCCTTCAGGACGAACAGAGCGGCCTCATCCTTACAGCCAAGG
CCTGGCGCATGGCCCAAACGCTGACAGCTTCAATGATCGGCAAGCGCATCCGCGTAGCTTACACTCCCCGCCTTGATACC
TACAACGGCATAACGTCCGTTGATGTGGGCATCAAGGATTGGCAACCCGCATCCTGA

Upstream 100 bases:

>100_bases
TTGTACCCGCCGACAAGGTGGACGGAGTGCGCGCCGCCCTGCAGGCCGTAACCCAGAACGCCGGCAGCGCCTCCTGAGAC
AGGCAGGCCCCAGAGGTTTC

Downstream 100 bases:

>100_bases
CCACTGCCAGCAGATGGTCGTGACAGACAGTGGGCGGTAAAAAACTTTCTGTCTGTCGCAGGGTTTTCGCCTTTCCACAT
TTTCCCGCTGCACAGCCTGC

Product: single-stranded-DNA-specific exonuclease RecJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MKNWLFRTPPAGGPVCLPASWAEELSISPLLLEVLWRRGFSHKQAINAFLDARLDSLTPPHRWPQMPEAAQLLADELLAG
KKLAVWGDYDVDGITASTLVLDVLESHGIEALWHIPDRRTEGYGLNVKQIDALAAQGCRILLTVDCGIADVDAVNHARRI
GMTVVVSDHHLPQDDLPPAHALCNPRMCPEESLPCPHLAGVGVAFYLMGAVNAALAPHTGKKHRMDKCLDLVALGTLADI
MPLTSENRILVRGGLGHLADASRPGIAALKVASGMDAKTRLTSGQVSFQLVPRINAAGRIGDGGLALRLLRQKDFSSALP
LAQQLDQLNTERKQAENTIHSEARAQVLDMLSREPRASLVLYGQDWHPGIVGIVASRIVEEFYRPTIILCQDQGSLKGSG
RSVREFDLHAGLCQTASSLSGFGGHRLAAGVRLPLENLARFRTEFENFVAQQLGTNPLSPSLTLECQLDFAQASNQSFLK
ELEMLQPFGPGNAEPVFASPPLLVKERSPLGRGGEHVRLRLQDEQSGLILTAKAWRMAQTLTASMIGKRIRVAYTPRLDT
YNGITSVDVGIKDWQPAS

Sequences:

>Translated_578_residues
MKNWLFRTPPAGGPVCLPASWAEELSISPLLLEVLWRRGFSHKQAINAFLDARLDSLTPPHRWPQMPEAAQLLADELLAG
KKLAVWGDYDVDGITASTLVLDVLESHGIEALWHIPDRRTEGYGLNVKQIDALAAQGCRILLTVDCGIADVDAVNHARRI
GMTVVVSDHHLPQDDLPPAHALCNPRMCPEESLPCPHLAGVGVAFYLMGAVNAALAPHTGKKHRMDKCLDLVALGTLADI
MPLTSENRILVRGGLGHLADASRPGIAALKVASGMDAKTRLTSGQVSFQLVPRINAAGRIGDGGLALRLLRQKDFSSALP
LAQQLDQLNTERKQAENTIHSEARAQVLDMLSREPRASLVLYGQDWHPGIVGIVASRIVEEFYRPTIILCQDQGSLKGSG
RSVREFDLHAGLCQTASSLSGFGGHRLAAGVRLPLENLARFRTEFENFVAQQLGTNPLSPSLTLECQLDFAQASNQSFLK
ELEMLQPFGPGNAEPVFASPPLLVKERSPLGRGGEHVRLRLQDEQSGLILTAKAWRMAQTLTASMIGKRIRVAYTPRLDT
YNGITSVDVGIKDWQPAS
>Mature_578_residues
MKNWLFRTPPAGGPVCLPASWAEELSISPLLLEVLWRRGFSHKQAINAFLDARLDSLTPPHRWPQMPEAAQLLADELLAG
KKLAVWGDYDVDGITASTLVLDVLESHGIEALWHIPDRRTEGYGLNVKQIDALAAQGCRILLTVDCGIADVDAVNHARRI
GMTVVVSDHHLPQDDLPPAHALCNPRMCPEESLPCPHLAGVGVAFYLMGAVNAALAPHTGKKHRMDKCLDLVALGTLADI
MPLTSENRILVRGGLGHLADASRPGIAALKVASGMDAKTRLTSGQVSFQLVPRINAAGRIGDGGLALRLLRQKDFSSALP
LAQQLDQLNTERKQAENTIHSEARAQVLDMLSREPRASLVLYGQDWHPGIVGIVASRIVEEFYRPTIILCQDQGSLKGSG
RSVREFDLHAGLCQTASSLSGFGGHRLAAGVRLPLENLARFRTEFENFVAQQLGTNPLSPSLTLECQLDFAQASNQSFLK
ELEMLQPFGPGNAEPVFASPPLLVKERSPLGRGGEHVRLRLQDEQSGLILTAKAWRMAQTLTASMIGKRIRVAYTPRLDT
YNGITSVDVGIKDWQPAS

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=569, Percent_Identity=32.688927943761, Blast_Score=257, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 62866; Mature: 62866

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNWLFRTPPAGGPVCLPASWAEELSISPLLLEVLWRRGFSHKQAINAFLDARLDSLTPP
CCCCCCCCCCCCCCEEECCCHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
HRWPQMPEAAQLLADELLAGKKLAVWGDYDVDGITASTLVLDVLESHGIEALWHIPDRRT
CCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCC
EGYGLNVKQIDALAAQGCRILLTVDCGIADVDAVNHARRIGMTVVVSDHHLPQDDLPPAH
CCCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCHH
ALCNPRMCPEESLPCPHLAGVGVAFYLMGAVNAALAPHTGKKHRMDKCLDLVALGTLADI
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
MPLTSENRILVRGGLGHLADASRPGIAALKVASGMDAKTRLTSGQVSFQLVPRINAAGRI
HCCCCCCEEEEECCCCHHCCCCCCCEEEEEECCCCCHHHEECCCCEEEEEEECCCCCCCC
GDGGLALRLLRQKDFSSALPLAQQLDQLNTERKQAENTIHSEARAQVLDMLSREPRASLV
CCCHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
LYGQDWHPGIVGIVASRIVEEFYRPTIILCQDQGSLKGSGRSVREFDLHAGLCQTASSLS
EEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
GFGGHRLAAGVRLPLENLARFRTEFENFVAQQLGTNPLSPSLTLECQLDFAQASNQSFLK
CCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEHHHCCCHHHHH
ELEMLQPFGPGNAEPVFASPPLLVKERSPLGRGGEHVRLRLQDEQSGLILTAKAWRMAQT
HHHHHCCCCCCCCCCEECCCCEEEECCCCCCCCCCEEEEEEEECCCCEEEEHHHHHHHHH
LTASMIGKRIRVAYTPRLDTYNGITSVDVGIKDWQPAS
HHHHHHCCEEEEEECCCCCCCCCCEEEECCCCCCCCCC
>Mature Secondary Structure
MKNWLFRTPPAGGPVCLPASWAEELSISPLLLEVLWRRGFSHKQAINAFLDARLDSLTPP
CCCCCCCCCCCCCCEEECCCHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
HRWPQMPEAAQLLADELLAGKKLAVWGDYDVDGITASTLVLDVLESHGIEALWHIPDRRT
CCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCC
EGYGLNVKQIDALAAQGCRILLTVDCGIADVDAVNHARRIGMTVVVSDHHLPQDDLPPAH
CCCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCHH
ALCNPRMCPEESLPCPHLAGVGVAFYLMGAVNAALAPHTGKKHRMDKCLDLVALGTLADI
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
MPLTSENRILVRGGLGHLADASRPGIAALKVASGMDAKTRLTSGQVSFQLVPRINAAGRI
HCCCCCCEEEEECCCCHHCCCCCCCEEEEEECCCCCHHHEECCCCEEEEEEECCCCCCCC
GDGGLALRLLRQKDFSSALPLAQQLDQLNTERKQAENTIHSEARAQVLDMLSREPRASLV
CCCHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
LYGQDWHPGIVGIVASRIVEEFYRPTIILCQDQGSLKGSGRSVREFDLHAGLCQTASSLS
EEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
GFGGHRLAAGVRLPLENLARFRTEFENFVAQQLGTNPLSPSLTLECQLDFAQASNQSFLK
CCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEHHHCCCHHHHH
ELEMLQPFGPGNAEPVFASPPLLVKERSPLGRGGEHVRLRLQDEQSGLILTAKAWRMAQT
HHHHHCCCCCCCCCCEECCCCEEEECCCCCCCCCCEEEEEEEECCCCEEEEHHHHHHHHH
LTASMIGKRIRVAYTPRLDTYNGITSVDVGIKDWQPAS
HHHHHHCCEEEEEECCCCCCCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]