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The map label for this gene is hldE [H]

Identifier: 220904659

GI number: 220904659

Start: 1664579

End: 1666045

Strand: Reverse

Name: hldE [H]

Synonym: Ddes_1391

Alternate gene names: 220904659

Gene position: 1666045-1664579 (Counterclockwise)

Preceding gene: 220904660

Following gene: 220904658

Centisome position: 57.98

GC content: 59.44

Gene sequence:

>1467_bases
ATGAATTTTGATACGGTGCATGTGCTGGTAGTGGGCGACGTAATGCTTGACCGCTACATTGCCGGGAGTGTGGGGCGCAT
ATCGCCTGAAGCCCCGGTGCCTGTACTTGGCGTGAACAAACGATGGTTCGCGCCGGGCGGCGCGGCCAATGTGGCCCGCA
ATCTGGTGAACCTTGGAGCGCAGGCCACCCTTGTGGGCCTTTCCGGCATTGATGAAGCCGGCGACGATCTGTGTCGGAGC
CTGGAGCAGGCCGGCATTGAGCACCGCCTGATATCTTCCGGAGCGCGCAGCACCACATGCAAGACCAGAGTGCTCGCCCA
GGGGCAGCAGTTGCTGCGGCTGGATGAAGAGCGCTGCATGCACCCGGACGCGGATGAGCTTGATGCCTTGCGCCAGGCGG
TGTTTGATACGCTGCCTGATTGCCGTGCAGTGATCCTTTCTGATTACGGCAAGGGTGTTTTGCTGGAAAACGAGCAGGGT
GAAAGCCTGTGCCGCGCTATTATCGACGAAGCCCGCCGCCTGGGTATTGCCGTGCTTGTGGATCCCAAGGGAACGCAGTG
GGGCCGCTATGCGGGTGCGCATTGCGTAACGCCCAACAGGCAGGAATTTGCTCTGGCCTGTGGCAGGGACGAACATGTGC
CGCTGGACAGGGCGCTGAGGGCAGAACTGGCAGACCGGCTGCGGAAACGCCATGCCATTGAAAGAGTGCTGCTGACCCGT
GGGGCCAAGGGAATGGCCCTGTTCGGCCCGGATGCACCTCCACGTTATATACGAGCCGCCATGCGCGAAGTTGCGGATGT
TTCCGGAGCGGGCGATACTGTGATTGCCACTCTGGGGGCCTGCATCGGCAGCGGCTTGTCCTGGGAAGAAAGCATGGATG
CGGCCAATGCCGCGGCCGGCGTTGCTGTGGGAAAAATGGGTACGGCTCCTGTAAGTATTACCGAATTGCGTCAGGCGCTG
CGTCAGGGCGCGGATAATCCCAAGCTTTATGCCTTGCCAGAGCTTCTTGAAAAGGTGGAAGACTGGCGGCGAAAAAATGA
AAGCATAGTCTTCACCAACGGATGTTTTGACTTACTGCATCCGGGTCATATTTCTCTTTTACGCCAAAGTGCCGCTCAGG
GAACCCACCTTGTGGTGGGGCTTAATTCCGATGCCTCGGTACGCCGTCTCAAGGGGCCAAGCCGCCCGGTACAAAACGAG
CAAAGCCGCGCGTTGCTTTTGTCCGCTCTGCACGGAGTGGACGCAGTCGTGTTTTTTGACGAAGACACACCGCTGAATCT
TGTCACCGCCCTGCGTCCTGATGTACTCGTCAAAGGCAGTGACTATACGGTGGAAACCGTAGTGGGGGCGGATGTGGTGC
TGGCTTCGGGCGGCCGCGTCTATCTGGCCGATCTTGTGCAGGGGTGCAGTACCACCGGGATTGTGCGCAAAATTGACGCT
TCCGGCGGGAACAGCTCCTCACGTTGA

Upstream 100 bases:

>100_bases
AGAGGGCGTTGAGGCCCGTATGAGCACGTTTTTTAAAAAGGCGTGCCGCAAGCTGTGAGCAACAACATCTTGTGAGTCCG
CATCCTGAAACGGAAATGCC

Downstream 100 bases:

>100_bases
GTGACTTGTCATGCAGCGAATTCTTGTGGCCGATGTGGGGGGAACCAACTGCCGGATGGGCAGCTTCAGGCTGGAGGGAA
CCTCGCTTACGCTTGAATGC

Product: rfaE bifunctional protein

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MNFDTVHVLVVGDVMLDRYIAGSVGRISPEAPVPVLGVNKRWFAPGGAANVARNLVNLGAQATLVGLSGIDEAGDDLCRS
LEQAGIEHRLISSGARSTTCKTRVLAQGQQLLRLDEERCMHPDADELDALRQAVFDTLPDCRAVILSDYGKGVLLENEQG
ESLCRAIIDEARRLGIAVLVDPKGTQWGRYAGAHCVTPNRQEFALACGRDEHVPLDRALRAELADRLRKRHAIERVLLTR
GAKGMALFGPDAPPRYIRAAMREVADVSGAGDTVIATLGACIGSGLSWEESMDAANAAAGVAVGKMGTAPVSITELRQAL
RQGADNPKLYALPELLEKVEDWRRKNESIVFTNGCFDLLHPGHISLLRQSAAQGTHLVVGLNSDASVRRLKGPSRPVQNE
QSRALLLSALHGVDAVVFFDEDTPLNLVTALRPDVLVKGSDYTVETVVGADVVLASGGRVYLADLVQGCSTTGIVRKIDA
SGGNSSSR

Sequences:

>Translated_488_residues
MNFDTVHVLVVGDVMLDRYIAGSVGRISPEAPVPVLGVNKRWFAPGGAANVARNLVNLGAQATLVGLSGIDEAGDDLCRS
LEQAGIEHRLISSGARSTTCKTRVLAQGQQLLRLDEERCMHPDADELDALRQAVFDTLPDCRAVILSDYGKGVLLENEQG
ESLCRAIIDEARRLGIAVLVDPKGTQWGRYAGAHCVTPNRQEFALACGRDEHVPLDRALRAELADRLRKRHAIERVLLTR
GAKGMALFGPDAPPRYIRAAMREVADVSGAGDTVIATLGACIGSGLSWEESMDAANAAAGVAVGKMGTAPVSITELRQAL
RQGADNPKLYALPELLEKVEDWRRKNESIVFTNGCFDLLHPGHISLLRQSAAQGTHLVVGLNSDASVRRLKGPSRPVQNE
QSRALLLSALHGVDAVVFFDEDTPLNLVTALRPDVLVKGSDYTVETVVGADVVLASGGRVYLADLVQGCSTTGIVRKIDA
SGGNSSSR
>Mature_488_residues
MNFDTVHVLVVGDVMLDRYIAGSVGRISPEAPVPVLGVNKRWFAPGGAANVARNLVNLGAQATLVGLSGIDEAGDDLCRS
LEQAGIEHRLISSGARSTTCKTRVLAQGQQLLRLDEERCMHPDADELDALRQAVFDTLPDCRAVILSDYGKGVLLENEQG
ESLCRAIIDEARRLGIAVLVDPKGTQWGRYAGAHCVTPNRQEFALACGRDEHVPLDRALRAELADRLRKRHAIERVLLTR
GAKGMALFGPDAPPRYIRAAMREVADVSGAGDTVIATLGACIGSGLSWEESMDAANAAAGVAVGKMGTAPVSITELRQAL
RQGADNPKLYALPELLEKVEDWRRKNESIVFTNGCFDLLHPGHISLLRQSAAQGTHLVVGLNSDASVRRLKGPSRPVQNE
QSRALLLSALHGVDAVVFFDEDTPLNLVTALRPDVLVKGSDYTVETVVGADVVLASGGRVYLADLVQGCSTTGIVRKIDA
SGGNSSSR

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505651, Length=132, Percent_Identity=34.8484848484849, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI296841136, Length=132, Percent_Identity=34.8484848484849, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1789432, Length=479, Percent_Identity=44.4676409185804, Blast_Score=362, Evalue=1e-101,
Organism=Drosophila melanogaster, GI45550974, Length=136, Percent_Identity=31.6176470588235, Blast_Score=66, Evalue=6e-11,
Organism=Drosophila melanogaster, GI45550975, Length=136, Percent_Identity=31.6176470588235, Blast_Score=66, Evalue=6e-11,
Organism=Drosophila melanogaster, GI45550973, Length=136, Percent_Identity=31.6176470588235, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.-

Molecular weight: Translated: 52123; Mature: 52123

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFDTVHVLVVGDVMLDRYIAGSVGRISPEAPVPVLGVNKRWFAPGGAANVARNLVNLGA
CCCCEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCC
QATLVGLSGIDEAGDDLCRSLEQAGIEHRLISSGARSTTCKTRVLAQGQQLLRLDEERCM
CEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHCCHHHHHHCHHHCC
HPDADELDALRQAVFDTLPDCRAVILSDYGKGVLLENEQGESLCRAIIDEARRLGIAVLV
CCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCEEEEE
DPKGTQWGRYAGAHCVTPNRQEFALACGRDEHVPLDRALRAELADRLRKRHAIERVLLTR
CCCCCCCCCCCCCCCCCCCCCHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GAKGMALFGPDAPPRYIRAAMREVADVSGAGDTVIATLGACIGSGLSWEESMDAANAAAG
CCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VAVGKMGTAPVSITELRQALRQGADNPKLYALPELLEKVEDWRRKNESIVFTNGCFDLLH
HEECCCCCCCCCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCEEEECCCHHHCC
PGHISLLRQSAAQGTHLVVGLNSDASVRRLKGPSRPVQNEQSRALLLSALHGVDAVVFFD
CCHHHHHHHHHCCCCEEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEEEC
EDTPLNLVTALRPDVLVKGSDYTVETVVGADVVLASGGRVYLADLVQGCSTTGIVRKIDA
CCCCCHHHHHCCCCEEEECCCCEEEEEECCEEEEECCCCEEHHHHHCCCCCCCCEEEEEC
SGGNSSSR
CCCCCCCC
>Mature Secondary Structure
MNFDTVHVLVVGDVMLDRYIAGSVGRISPEAPVPVLGVNKRWFAPGGAANVARNLVNLGA
CCCCEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCC
QATLVGLSGIDEAGDDLCRSLEQAGIEHRLISSGARSTTCKTRVLAQGQQLLRLDEERCM
CEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHCCHHHHHHCHHHCC
HPDADELDALRQAVFDTLPDCRAVILSDYGKGVLLENEQGESLCRAIIDEARRLGIAVLV
CCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCEEEEE
DPKGTQWGRYAGAHCVTPNRQEFALACGRDEHVPLDRALRAELADRLRKRHAIERVLLTR
CCCCCCCCCCCCCCCCCCCCCHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GAKGMALFGPDAPPRYIRAAMREVADVSGAGDTVIATLGACIGSGLSWEESMDAANAAAG
CCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VAVGKMGTAPVSITELRQALRQGADNPKLYALPELLEKVEDWRRKNESIVFTNGCFDLLH
HEECCCCCCCCCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCEEEECCCHHHCC
PGHISLLRQSAAQGTHLVVGLNSDASVRRLKGPSRPVQNEQSRALLLSALHGVDAVVFFD
CCHHHHHHHHHCCCCEEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEEEC
EDTPLNLVTALRPDVLVKGSDYTVETVVGADVVLASGGRVYLADLVQGCSTTGIVRKIDA
CCCCCHHHHHCCCCEEEECCCCEEEEEECCEEEEECCCCEEHHHHHCCCCCCCCEEEEEC
SGGNSSSR
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA