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The map label for this gene is pchA [H]

Identifier: 220904613

GI number: 220904613

Start: 1614892

End: 1616295

Strand: Reverse

Name: pchA [H]

Synonym: Ddes_1345

Alternate gene names: 220904613

Gene position: 1616295-1614892 (Counterclockwise)

Preceding gene: 220904614

Following gene: 220904612

Centisome position: 56.25

GC content: 55.34

Gene sequence:

>1404_bases
ATGGTTGATGCATTACGCCAGCCTCTGCGCATCGCCATGCACAAAGCTTGTGAGTCTGCCGCAAGGCTGGAGCGAGCCAT
CCTTGCCGTAGCGAGCATAGTTGTCCCCCGCATTGATATGTTTTCAGTATTTGCACGTTATTCTTCACAGATGGATGCAA
ATATTTGGCTTGGTCTGGCAGGAAGTTCCATGCTCGGCCTTGGTGCTGCCCTTGAGTGCACAGGGGAGGGGAAGGACAGA
TTCGCAACTATTTCAGCTTGCTGGCACGATGCTGTCAGGCACGCCGTGATTGAAGGGGATGCGCCTCCTGTGGCTTTCGG
GGGATTTCGGTTTGACGCCGCGCGGCCCGCGTCTTCAGTATGGCCAGGGTTTGGAGACGGAGCGCTGGTTGTTCCCCGTC
TGACTGTCGTGCAATACGGGCGGGATTGTTGTCGCGTTATAGCTGCGGAGCAGATGTTCCCTGGCTCAGATGCTGTTGCT
CGGGCAGAAGCTGCTCTTTCCCTTTGGGAAGGTCTGGCTGGCGGCAATGCCATACATCCCACGCATAGGAACGCACATGT
CCAGGTACTCATTCCAAAAAATGCAAAAAACCAATGGTGCGCTCTGGTCCATGCAGCCTTGCATAGCATCTCACAGGGTG
ATGCAAGAAAGATTGTGGTAGCTCGCACACTCAAGGCCAAGGCCGCAGATCCTTTCACACTGCCAGATATTCTCGACAGG
TTGCGCTCTGCTAATCCCAAGGCAGCCATATTCGCATTTGGTCGCAAGGGAACCTACTTTGTCGGCGCAACGCCAGAAAT
ACTCATCACTGCCCATAGTGGAACATTTCGGACCATGGCTCTTGCCGGAAGTGCTGCCCGCAGTGAGACCCCCCAACAAG
ACGCCAGGTTGGGGCAAGCGCTTCTGACCTCTCCCAAGGACCGCCTGGAGCACGATTTCGTGGTGCAGGCAGTGCTACAG
GCGCTTCAATCCTGCTGCAGCAGGATTGAAGCGGACTCTTCGCCAAGCCTGCACAAACTGCCCACGGTGCAACACCTCGT
CACATTTTTTAGAGGGGATATTTTGCCCCACAAGAGTCTCCTGGATGTAATTGCACGCTTGCATCCCACACCAGCGGTGG
GAGGAGTTCCAGCCGGTTCCGCGCTGGCATTTTTGCGCGACCATGAAGGTTTTGACAGGGGATGGTACGCCGGGCCTGTG
GGCTGGCTTGATGCCGAGGGAAATGGTGAGTTTATGGTTGCCCTCCGCTCTGGGGTAATACAGGGGAATGAGGCTGTTCT
TTTTGCCGGTTGTGGCCTTGTGGCAGGTTCCGATCCTGAAAAGGAATATCAGGAGACCTGGATCAAGTTTTCAACCATGC
TCAATGGCCTGTGCCCGGCCCAAGGTGCTGAATTCGTCATTTGA

Upstream 100 bases:

>100_bases
GATTTTATTGAAAATTTATTTAAGCAGATCACCGCCTGGTATATCGCAACCCAAGTTGCGCACTGGCGAAAAGTGCACGC
TCTGGATGGGGAAAAGAGCG

Downstream 100 bases:

>100_bases
AGGAATATTAGAGGAATTACCGCTTCAAATGATTGCTTAACGGTCGGCAAAGCCGCCCTGCAATCATTTGGCTACAAGGA
TTTGGAGGCAAATCCTTGTA

Product: isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MVDALRQPLRIAMHKACESAARLERAILAVASIVVPRIDMFSVFARYSSQMDANIWLGLAGSSMLGLGAALECTGEGKDR
FATISACWHDAVRHAVIEGDAPPVAFGGFRFDAARPASSVWPGFGDGALVVPRLTVVQYGRDCCRVIAAEQMFPGSDAVA
RAEAALSLWEGLAGGNAIHPTHRNAHVQVLIPKNAKNQWCALVHAALHSISQGDARKIVVARTLKAKAADPFTLPDILDR
LRSANPKAAIFAFGRKGTYFVGATPEILITAHSGTFRTMALAGSAARSETPQQDARLGQALLTSPKDRLEHDFVVQAVLQ
ALQSCCSRIEADSSPSLHKLPTVQHLVTFFRGDILPHKSLLDVIARLHPTPAVGGVPAGSALAFLRDHEGFDRGWYAGPV
GWLDAEGNGEFMVALRSGVIQGNEAVLFAGCGLVAGSDPEKEYQETWIKFSTMLNGLCPAQGAEFVI

Sequences:

>Translated_467_residues
MVDALRQPLRIAMHKACESAARLERAILAVASIVVPRIDMFSVFARYSSQMDANIWLGLAGSSMLGLGAALECTGEGKDR
FATISACWHDAVRHAVIEGDAPPVAFGGFRFDAARPASSVWPGFGDGALVVPRLTVVQYGRDCCRVIAAEQMFPGSDAVA
RAEAALSLWEGLAGGNAIHPTHRNAHVQVLIPKNAKNQWCALVHAALHSISQGDARKIVVARTLKAKAADPFTLPDILDR
LRSANPKAAIFAFGRKGTYFVGATPEILITAHSGTFRTMALAGSAARSETPQQDARLGQALLTSPKDRLEHDFVVQAVLQ
ALQSCCSRIEADSSPSLHKLPTVQHLVTFFRGDILPHKSLLDVIARLHPTPAVGGVPAGSALAFLRDHEGFDRGWYAGPV
GWLDAEGNGEFMVALRSGVIQGNEAVLFAGCGLVAGSDPEKEYQETWIKFSTMLNGLCPAQGAEFVI
>Mature_467_residues
MVDALRQPLRIAMHKACESAARLERAILAVASIVVPRIDMFSVFARYSSQMDANIWLGLAGSSMLGLGAALECTGEGKDR
FATISACWHDAVRHAVIEGDAPPVAFGGFRFDAARPASSVWPGFGDGALVVPRLTVVQYGRDCCRVIAAEQMFPGSDAVA
RAEAALSLWEGLAGGNAIHPTHRNAHVQVLIPKNAKNQWCALVHAALHSISQGDARKIVVARTLKAKAADPFTLPDILDR
LRSANPKAAIFAFGRKGTYFVGATPEILITAHSGTFRTMALAGSAARSETPQQDARLGQALLTSPKDRLEHDFVVQAVLQ
ALQSCCSRIEADSSPSLHKLPTVQHLVTFFRGDILPHKSLLDVIARLHPTPAVGGVPAGSALAFLRDHEGFDRGWYAGPV
GWLDAEGNGEFMVALRSGVIQGNEAVLFAGCGLVAGSDPEKEYQETWIKFSTMLNGLCPAQGAEFVI

Specific function: Involved in the conversion of chorismate to salicylate (Probable) [H]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=266, Percent_Identity=37.218045112782, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI87082077, Length=264, Percent_Identity=34.469696969697, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1788114, Length=225, Percent_Identity=26.2222222222222, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787518, Length=283, Percent_Identity=24.0282685512367, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320935, Length=261, Percent_Identity=31.0344827586207, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 49887; Mature: 49887

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDALRQPLRIAMHKACESAARLERAILAVASIVVPRIDMFSVFARYSSQMDANIWLGLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEC
GSSMLGLGAALECTGEGKDRFATISACWHDAVRHAVIEGDAPPVAFGGFRFDAARPASSV
CCCHHCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHCCCCCCCEEECCEECCCCCCCCCC
WPGFGDGALVVPRLTVVQYGRDCCRVIAAEQMFPGSDAVARAEAALSLWEGLAGGNAIHP
CCCCCCCCEEHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
THRNAHVQVLIPKNAKNQWCALVHAALHSISQGDARKIVVARTLKAKAADPFTLPDILDR
CCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHCCCCCCCHHHHHHH
LRSANPKAAIFAFGRKGTYFVGATPEILITAHSGTFRTMALAGSAARSETPQQDARLGQA
HHCCCCCEEEEEECCCCCEEECCCCEEEEEECCCCEEEHEECCCHHCCCCCHHHHHHHHH
LLTSPKDRLEHDFVVQAVLQALQSCCSRIEADSSPSLHKLPTVQHLVTFFRGDILPHKSL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHHHCCCCCHHHH
LDVIARLHPTPAVGGVPAGSALAFLRDHEGFDRGWYAGPVGWLDAEGNGEFMVALRSGVI
HHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCEEEEEECCCC
QGNEAVLFAGCGLVAGSDPEKEYQETWIKFSTMLNGLCPAQGAEFVI
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MVDALRQPLRIAMHKACESAARLERAILAVASIVVPRIDMFSVFARYSSQMDANIWLGLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEC
GSSMLGLGAALECTGEGKDRFATISACWHDAVRHAVIEGDAPPVAFGGFRFDAARPASSV
CCCHHCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHCCCCCCCEEECCEECCCCCCCCCC
WPGFGDGALVVPRLTVVQYGRDCCRVIAAEQMFPGSDAVARAEAALSLWEGLAGGNAIHP
CCCCCCCCEEHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
THRNAHVQVLIPKNAKNQWCALVHAALHSISQGDARKIVVARTLKAKAADPFTLPDILDR
CCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHCCCCCCCHHHHHHH
LRSANPKAAIFAFGRKGTYFVGATPEILITAHSGTFRTMALAGSAARSETPQQDARLGQA
HHCCCCCEEEEEECCCCCEEECCCCEEEEEECCCCEEEHEECCCHHCCCCCHHHHHHHHH
LLTSPKDRLEHDFVVQAVLQALQSCCSRIEADSSPSLHKLPTVQHLVTFFRGDILPHKSL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHHHCCCCCHHHH
LDVIARLHPTPAVGGVPAGSALAFLRDHEGFDRGWYAGPVGWLDAEGNGEFMVALRSGVI
HHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCEEEEEECCCC
QGNEAVLFAGCGLVAGSDPEKEYQETWIKFSTMLNGLCPAQGAEFVI
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7500944; 10984043 [H]