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The map label for this gene is nifA [H]

Identifier: 220904573

GI number: 220904573

Start: 1545968

End: 1547107

Strand: Reverse

Name: nifA [H]

Synonym: Ddes_1305

Alternate gene names: 220904573

Gene position: 1547107-1545968 (Counterclockwise)

Preceding gene: 220904575

Following gene: 220904572

Centisome position: 53.84

GC content: 54.65

Gene sequence:

>1140_bases
ATGGCGTTGAATACAGACGGCATTATTGGCCAGAGCACAAGCCTGGCAGAGGTTTTTAAAGTTCTCGGCAAGGTTGCGCC
TACCGACAGCACTGTGCTTGTAACCGGGGAATCCGGAACAGGCAAGGAATTGCTGGTGCGCGCCCTGCACTCCAACAGCA
GCCGCGAAGATAAGCCCTTTGTTCCCATCAACTGCGGAGCCATACCTAAAGAATTGCTTGAAAGTGAACTTTTCGGACAT
GAAAAGGGAGCATTTACACACGCCATTCGTTCCCGTCCGGGGCGTTTTGAGCTGGCCGACGGCGGCACGATATTTCTTGA
CGAAATAGGCGAAATGGACCTGAGCCTTCAGGTCAAGATATTGCGGGTGCTCCAGGAAAAGGAGATCGAACGCGTTGGGG
GAAGTGGCAGCAAAAAAGTGGACGTACGCATTGTGGCGGCCACCAACCGTGACCTTGAAGCTGAGGTGGCTGCCGGGCGC
TTTCGCGAAGACCTTTATTATCGCCTCAATGTCATCCCGCTGCACCTGCCGCCCCTGCGTGAAAGAGGGGGTGATGTGCT
CGTGCTGGCGCGGCACTTTCTGACCCATTTTTGCGGCAAGAAAAAGCGCCCGGTTCTTGTCCTTGACGATGATGCGCGCC
GTGTGCTTTCTGCCTACACTTGGCCCGGCAATGTGCGCGAGCTTGAAAATTTCATGGAACGGTTGAGCATTCTTGTAGAT
GGGGATGTGGTACACCCGCAGGACCTGCCCGGCAAGATACTTGACCAGGTAGGAGATATTGCATCCCTGCCGGAAGTGGA
AGATATTGCCGCAGGTGCTGATGAAGCAGGTGCTGTCGAAAATATTGTTGCCGCTGCGCCCTCTACGGGGATGGATGCGG
TTGAAAATGAAAAAATGGCGGCAGCCTCCGCTGCCGCGCCGGACAATGGAGCGGGCCAGCAGGCGGGTACTTTTGTCTGG
CCTGACTTGAGCGTGCTGGAAGCGCGTGGTCTGACTCTGAAAGATTTTCTTGATGCCGTTGAAGGGCGCCTTATTGATGA
AGCACTGGATAAAGCTCTGGGCGTCAAGAATCAGGCGGCAGAACTACTGGGCATCAAGCGTACGACTCTTATTGAGAAAC
TCAAAAAGCGCAATGCGTGA

Upstream 100 bases:

>100_bases
ACAGGTTGGTTAAACGGAATGTCAAAACAGCTTTTTTACTGGTAATCCTGTTCGCGTCAGTGTATTGACACAAGAAAGGA
TTACTGGTGGAGAAAAATTC

Downstream 100 bases:

>100_bases
TGGTATATATGTTGCATTACGCATAACGGTTCCTGACTTTCAACCCTCTTCGCGAAGCGCACGTGAACATGATTTTTTTT
ACCGGCACAGGCCGTATTCA

Product: Fis family sigma-54 specific transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MALNTDGIIGQSTSLAEVFKVLGKVAPTDSTVLVTGESGTGKELLVRALHSNSSREDKPFVPINCGAIPKELLESELFGH
EKGAFTHAIRSRPGRFELADGGTIFLDEIGEMDLSLQVKILRVLQEKEIERVGGSGSKKVDVRIVAATNRDLEAEVAAGR
FREDLYYRLNVIPLHLPPLRERGGDVLVLARHFLTHFCGKKKRPVLVLDDDARRVLSAYTWPGNVRELENFMERLSILVD
GDVVHPQDLPGKILDQVGDIASLPEVEDIAAGADEAGAVENIVAAAPSTGMDAVENEKMAAASAAAPDNGAGQQAGTFVW
PDLSVLEARGLTLKDFLDAVEGRLIDEALDKALGVKNQAAELLGIKRTTLIEKLKKRNA

Sequences:

>Translated_379_residues
MALNTDGIIGQSTSLAEVFKVLGKVAPTDSTVLVTGESGTGKELLVRALHSNSSREDKPFVPINCGAIPKELLESELFGH
EKGAFTHAIRSRPGRFELADGGTIFLDEIGEMDLSLQVKILRVLQEKEIERVGGSGSKKVDVRIVAATNRDLEAEVAAGR
FREDLYYRLNVIPLHLPPLRERGGDVLVLARHFLTHFCGKKKRPVLVLDDDARRVLSAYTWPGNVRELENFMERLSILVD
GDVVHPQDLPGKILDQVGDIASLPEVEDIAAGADEAGAVENIVAAAPSTGMDAVENEKMAAASAAAPDNGAGQQAGTFVW
PDLSVLEARGLTLKDFLDAVEGRLIDEALDKALGVKNQAAELLGIKRTTLIEKLKKRNA
>Mature_378_residues
ALNTDGIIGQSTSLAEVFKVLGKVAPTDSTVLVTGESGTGKELLVRALHSNSSREDKPFVPINCGAIPKELLESELFGHE
KGAFTHAIRSRPGRFELADGGTIFLDEIGEMDLSLQVKILRVLQEKEIERVGGSGSKKVDVRIVAATNRDLEAEVAAGRF
REDLYYRLNVIPLHLPPLRERGGDVLVLARHFLTHFCGKKKRPVLVLDDDARRVLSAYTWPGNVRELENFMERLSILVDG
DVVHPQDLPGKILDQVGDIASLPEVEDIAAGADEAGAVENIVAAAPSTGMDAVENEKMAAASAAAPDNGAGQQAGTFVWP
DLSVLEARGLTLKDFLDAVEGRLIDEALDKALGVKNQAAELLGIKRTTLIEKLKKRNA

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=260, Percent_Identity=51.9230769230769, Blast_Score=280, Evalue=9e-77,
Organism=Escherichia coli, GI1790437, Length=295, Percent_Identity=47.7966101694915, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI87082117, Length=235, Percent_Identity=56.1702127659575, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1790299, Length=372, Percent_Identity=42.2043010752688, Blast_Score=268, Evalue=3e-73,
Organism=Escherichia coli, GI1789087, Length=238, Percent_Identity=53.3613445378151, Blast_Score=254, Evalue=9e-69,
Organism=Escherichia coli, GI87082152, Length=237, Percent_Identity=51.8987341772152, Blast_Score=244, Evalue=7e-66,
Organism=Escherichia coli, GI1789233, Length=278, Percent_Identity=43.5251798561151, Blast_Score=234, Evalue=5e-63,
Organism=Escherichia coli, GI1788905, Length=239, Percent_Identity=47.2803347280335, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI87081872, Length=249, Percent_Identity=47.7911646586345, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI1786524, Length=310, Percent_Identity=41.6129032258065, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1787583, Length=242, Percent_Identity=42.1487603305785, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI87081858, Length=296, Percent_Identity=30.4054054054054, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1789828, Length=247, Percent_Identity=34.412955465587, Blast_Score=125, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 40890; Mature: 40759

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALNTDGIIGQSTSLAEVFKVLGKVAPTDSTVLVTGESGTGKELLVRALHSNSSREDKPF
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCE
VPINCGAIPKELLESELFGHEKGAFTHAIRSRPGRFELADGGTIFLDEIGEMDLSLQVKI
EEEECCCCHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCEEEEECCCCCCHHHHHHH
LRVLQEKEIERVGGSGSKKVDVRIVAATNRDLEAEVAAGRFREDLYYRLNVIPLHLPPLR
HHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHEEEEEEEEECCCCHH
ERGGDVLVLARHFLTHFCGKKKRPVLVLDDDARRVLSAYTWPGNVRELENFMERLSILVD
HCCCCEEEHHHHHHHHHHCCCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
GDVVHPQDLPGKILDQVGDIASLPEVEDIAAGADEAGAVENIVAAAPSTGMDAVENEKMA
CCEECCCCCCHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHCCHHH
AASAAAPDNGAGQQAGTFVWPDLSVLEARGLTLKDFLDAVEGRLIDEALDKALGVKNQAA
HHHCCCCCCCCCCCCCEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ELLGIKRTTLIEKLKKRNA
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALNTDGIIGQSTSLAEVFKVLGKVAPTDSTVLVTGESGTGKELLVRALHSNSSREDKPF
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCE
VPINCGAIPKELLESELFGHEKGAFTHAIRSRPGRFELADGGTIFLDEIGEMDLSLQVKI
EEEECCCCHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCEEEEECCCCCCHHHHHHH
LRVLQEKEIERVGGSGSKKVDVRIVAATNRDLEAEVAAGRFREDLYYRLNVIPLHLPPLR
HHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHEEEEEEEEECCCCHH
ERGGDVLVLARHFLTHFCGKKKRPVLVLDDDARRVLSAYTWPGNVRELENFMERLSILVD
HCCCCEEEHHHHHHHHHHCCCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
GDVVHPQDLPGKILDQVGDIASLPEVEDIAAGADEAGAVENIVAAAPSTGMDAVENEKMA
CCEECCCCCCHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHCCHHH
AASAAAPDNGAGQQAGTFVWPDLSVLEARGLTLKDFLDAVEGRLIDEALDKALGVKNQAA
HHHCCCCCCCCCCCCCEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ELLGIKRTTLIEKLKKRNA
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3027503 [H]