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The map label for this gene is bioB

Identifier: 220904569

GI number: 220904569

Start: 1539721

End: 1540671

Strand: Reverse

Name: bioB

Synonym: Ddes_1301

Alternate gene names: 220904569

Gene position: 1540671-1539721 (Counterclockwise)

Preceding gene: 220904570

Following gene: 220904568

Centisome position: 53.62

GC content: 54.99

Gene sequence:

>951_bases
ATGACAGAACTTTATACGGCGGCAAATCCCGCCGAGGCACTGAAGCTTGTATCGCTTCCGGAAGATGAGCTTTTTGTCGG
AGCTACGCAATTGCGCAGAGCGACTTTTGACAACAGGATAACGCTTTGTGCCATCATCAATGCACGTAGCGGCAACTGTG
GCATGGATTGCCGTTTTTGTTCACAGAGCAAACATAACCATACCCCAATTGAAACCTTTTCCCTTTTGCCCGACGATGAG
CTGCGAGGGCGAATCCTGGCGCTGGGCACGCAACCCGTAGCGCGCATAGGTGTGGTTACCAGTGGCGGGGCACTTAGCGG
TGAGGAATTTGACCGTTTGCTGGGAGTGCTGCGCTCCCTGCCGGAGTATGTACTCAAACGTGTTTGTGCATCTCTGGGCA
AGCTTTCTGCCGTTCAGCTTGCACAATTGATGGATATCGGTCTTGACCGATATCACCATAATCTTGAAACTTCCCGCCGG
TATTATCCCAGCATCTGTACCACACAAACATGGGACCAGCGCAAAGCAACGGTGGAACGTGTGTTCGGCGCAGGTATGAC
GGCCTGCACAGGTGGTCTGTTCGGCCTTGGAGAAAGCTGGCGTGACCGTATAGACTTTGCTTTTGCCCTCAAGTCGCTGG
GGGTGACGCATGTGCCCATGAATTTTCTGCATCCTCACCCGGAAACGCCGCTCGCGGGCCAGCCGCCGCTTACAGCCGGA
GAAGCCTTGCGCATTGTGGCTGTTTTCAGGCACATACTGCCCACGGCTACCTTGCGCATCTGCGGAGGCCGACCGCTGGT
GCTGGGGGCGCGGCAGAAAGAAATTTTTGCAGCCGGGGCGAATGCTCTGATGACTGGCGATTACCTGACTACTCAGGGCC
GCGGCCTGGTTGATGATCTTGCCATGATTGATTCTCTTGGTCTGGAGGTTGATTGTGACCAGAGCTGTTGA

Upstream 100 bases:

>100_bases
CCGACCGTTAAACAATCATTTGAAGCGTTAATTGCTCTAATTGCTGCGAGGCGCGGCGCAGGCAGGCTGTTCGCCGTCTT
CAGATTCCCAGGATCGGGTT

Downstream 100 bases:

>100_bases
AAAATGTTGCCCTGACCACATGTGCGAACCTTTGCGCGGCGTTTTTGTGGCGGGATCAGGTACGGACGTGGGTAAAACAG
TGTGCGCGGGCGCTTTGTTG

Product: Biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTELYTAANPAEALKLVSLPEDELFVGATQLRRATFDNRITLCAIINARSGNCGMDCRFCSQSKHNHTPIETFSLLPDDE
LRGRILALGTQPVARIGVVTSGGALSGEEFDRLLGVLRSLPEYVLKRVCASLGKLSAVQLAQLMDIGLDRYHHNLETSRR
YYPSICTTQTWDQRKATVERVFGAGMTACTGGLFGLGESWRDRIDFAFALKSLGVTHVPMNFLHPHPETPLAGQPPLTAG
EALRIVAVFRHILPTATLRICGGRPLVLGARQKEIFAAGANALMTGDYLTTQGRGLVDDLAMIDSLGLEVDCDQSC

Sequences:

>Translated_316_residues
MTELYTAANPAEALKLVSLPEDELFVGATQLRRATFDNRITLCAIINARSGNCGMDCRFCSQSKHNHTPIETFSLLPDDE
LRGRILALGTQPVARIGVVTSGGALSGEEFDRLLGVLRSLPEYVLKRVCASLGKLSAVQLAQLMDIGLDRYHHNLETSRR
YYPSICTTQTWDQRKATVERVFGAGMTACTGGLFGLGESWRDRIDFAFALKSLGVTHVPMNFLHPHPETPLAGQPPLTAG
EALRIVAVFRHILPTATLRICGGRPLVLGARQKEIFAAGANALMTGDYLTTQGRGLVDDLAMIDSLGLEVDCDQSC
>Mature_315_residues
TELYTAANPAEALKLVSLPEDELFVGATQLRRATFDNRITLCAIINARSGNCGMDCRFCSQSKHNHTPIETFSLLPDDEL
RGRILALGTQPVARIGVVTSGGALSGEEFDRLLGVLRSLPEYVLKRVCASLGKLSAVQLAQLMDIGLDRYHHNLETSRRY
YPSICTTQTWDQRKATVERVFGAGMTACTGGLFGLGESWRDRIDFAFALKSLGVTHVPMNFLHPHPETPLAGQPPLTAGE
ALRIVAVFRHILPTATLRICGGRPLVLGARQKEIFAAGANALMTGDYLTTQGRGLVDDLAMIDSLGLEVDCDQSC

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=305, Percent_Identity=31.8032786885246, Blast_Score=141, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6321725, Length=295, Percent_Identity=31.5254237288136, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_DESDA (B8J0C6)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479881.1
- GeneID:   7284993
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1301
- HOGENOM:   HBG405569
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 34358; Mature: 34227

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELYTAANPAEALKLVSLPEDELFVGATQLRRATFDNRITLCAIINARSGNCGMDCRFC
CCCCCCCCCHHHHHEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCC
SQSKHNHTPIETFSLLPDDELRGRILALGTQPVARIGVVTSGGALSGEEFDRLLGVLRSL
CCCCCCCCCCCEEECCCCHHHCCEEEEECCCCHHHEEEEECCCCCCHHHHHHHHHHHHHH
PEYVLKRVCASLGKLSAVQLAQLMDIGLDRYHHNLETSRRYYPSICTTQTWDQRKATVER
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
VFGAGMTACTGGLFGLGESWRDRIDFAFALKSLGVTHVPMNFLHPHPETPLAGQPPLTAG
HHCCCHHHHHCCHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCHH
EALRIVAVFRHILPTATLRICGGRPLVLGARQKEIFAAGANALMTGDYLTTQGRGLVDDL
HHHHHHHHHHHHHHHHHEEEECCCEEEECCCHHHHHHHCCCEEEECCEEECCCCCHHHHH
AMIDSLGLEVDCDQSC
HHHHHCCCEECCCCCC
>Mature Secondary Structure 
TELYTAANPAEALKLVSLPEDELFVGATQLRRATFDNRITLCAIINARSGNCGMDCRFC
CCCCCCCCHHHHHEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCC
SQSKHNHTPIETFSLLPDDELRGRILALGTQPVARIGVVTSGGALSGEEFDRLLGVLRSL
CCCCCCCCCCCEEECCCCHHHCCEEEEECCCCHHHEEEEECCCCCCHHHHHHHHHHHHHH
PEYVLKRVCASLGKLSAVQLAQLMDIGLDRYHHNLETSRRYYPSICTTQTWDQRKATVER
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
VFGAGMTACTGGLFGLGESWRDRIDFAFALKSLGVTHVPMNFLHPHPETPLAGQPPLTAG
HHCCCHHHHHCCHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCHH
EALRIVAVFRHILPTATLRICGGRPLVLGARQKEIFAAGANALMTGDYLTTQGRGLVDDL
HHHHHHHHHHHHHHHHHEEEECCCEEEECCCHHHHHHHCCCEEEECCEEECCCCCHHHHH
AMIDSLGLEVDCDQSC
HHHHHCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA