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The map label for this gene is ispDF [H]

Identifier: 220904521

GI number: 220904521

Start: 1471325

End: 1472587

Strand: Direct

Name: ispDF [H]

Synonym: Ddes_1252

Alternate gene names: 220904521

Gene position: 1471325-1472587 (Clockwise)

Preceding gene: 220904520

Following gene: 220904522

Centisome position: 51.2

GC content: 59.7

Gene sequence:

>1263_bases
ATGCCAGAAACTTCCGTGCCCCCCAGAGGGCAGCCCGTAAAGCCATGGGCCATACTGCTGGCCGCCGGACAGGGCCATCG
CCTTGCCGCCGCCACGGGCGGGCTTGCCAAGCAGTTTCTGCACTGGCAGGGAATGCCCCTGTATTGGCACGCCGCCAGAG
CCATGAGCCGCAGCGCGGCGGTGGCGGGCATTGTTTTTGTTTTTCCCCCTGCCGATTATGACGCACATGCAGAAAATCTG
CGCGCTCTTTATGCTGCTGGTGATTTGGGGTTGCCCTGCCTTACAGCAGTCGGCGGAGCACTGCGCCAGGACTCCGTACG
TCACGGGCTGGCCGCTTTGCCCGCAGATGTGAGCCATGTGCTTATTCATGATGCGGCCCGGCCTTTTGTCAGCCCGGCGT
TGATACGCAAGATATGTGCGTCCCTTGAATCCGGTGTACCGGGAGCAATCCCGGCCCTGCCGGTAACAGATACCATAAAG
ACTGTGTCACATGACCTGGTTACAGGCACCTTGCCTCGCCATACGCTCAGGGCAGTCCAGACGCCTCAGGGCTTCCGTCT
TGACCTTCTGCTTCAGGCCCATTGCCATGCGGAAAAACAGAAACTGAACGTCACTGACGATGCTTCCCTGCTGGAAGAGA
TGGGGTGTGAGATCCACGTGGTTGAAGGAGAAGCCGCCAACGTGAAAATTACCAACCCAGAGGATATGGATCTTTTGCGC
CCCCCCCATGCCCTGCCCCGCCCTTGCACGGGCATGGGCTACGATGTTCATCGCTACGCTCCGGGCGGCCGCCCCATGCG
GCTTGGCGGCGTGCTCATTCCCGCCGGGCCTGAGGTGGCCGCACATTCCGATGGCGATGTGCTGCTGCATGCTCTGACCG
ATGCAATACTCGGCTGTGCCGGTCTCGGCGATATAGGTCAGCACTTTTCTGATAAAGATCCCCGCTTTGATAATATTTCT
TCAGCGCTCCTGCTGGACGAAGTTCTGGATATGGTGCGCAAAAAAGGTGTGCAGTTGTGTCACGCCGACCTTACCCTAAT
TGCCCAGACGCCCCGTCTCGCGCCACACCGCGAAGAAATCAGAAAAAACATAGCCCGCCTGCTGGCTTTGCCCGCTGACT
GCGTCAACCTTAAAGCCAGCACAGAGGAAGGGCTTGGATTCACAGGCCGCGCCGAAGGCATCAAGGCATACGCCGTTGTC
ACAGCGTGGGCAGGCGCCCGCGCGGCCTTTGCGCCGCCGTCTTTTTTGCAACGCGACATCTGA

Upstream 100 bases:

>100_bases
ACCGCACTCCTAGAGGAATGACGGTCACACGCGGGCAACCGTGTGGACAGAACCCGGCTTACAGTCCGACTCCCGCATAC
TGTCAGCCAGAGGCCTAGCC

Downstream 100 bases:

>100_bases
TTCACAAGGATCGCCATGAACACAGAACTGTATCGTCCCTGGTTCGCTCACTACGATGCCTTTGTGCCGCGCATCACAGA
AGCATGGGATAAACCCCTCT

Product: 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MPETSVPPRGQPVKPWAILLAAGQGHRLAAATGGLAKQFLHWQGMPLYWHAARAMSRSAAVAGIVFVFPPADYDAHAENL
RALYAAGDLGLPCLTAVGGALRQDSVRHGLAALPADVSHVLIHDAARPFVSPALIRKICASLESGVPGAIPALPVTDTIK
TVSHDLVTGTLPRHTLRAVQTPQGFRLDLLLQAHCHAEKQKLNVTDDASLLEEMGCEIHVVEGEAANVKITNPEDMDLLR
PPHALPRPCTGMGYDVHRYAPGGRPMRLGGVLIPAGPEVAAHSDGDVLLHALTDAILGCAGLGDIGQHFSDKDPRFDNIS
SALLLDEVLDMVRKKGVQLCHADLTLIAQTPRLAPHREEIRKNIARLLALPADCVNLKASTEEGLGFTGRAEGIKAYAVV
TAWAGARAAFAPPSFLQRDI

Sequences:

>Translated_420_residues
MPETSVPPRGQPVKPWAILLAAGQGHRLAAATGGLAKQFLHWQGMPLYWHAARAMSRSAAVAGIVFVFPPADYDAHAENL
RALYAAGDLGLPCLTAVGGALRQDSVRHGLAALPADVSHVLIHDAARPFVSPALIRKICASLESGVPGAIPALPVTDTIK
TVSHDLVTGTLPRHTLRAVQTPQGFRLDLLLQAHCHAEKQKLNVTDDASLLEEMGCEIHVVEGEAANVKITNPEDMDLLR
PPHALPRPCTGMGYDVHRYAPGGRPMRLGGVLIPAGPEVAAHSDGDVLLHALTDAILGCAGLGDIGQHFSDKDPRFDNIS
SALLLDEVLDMVRKKGVQLCHADLTLIAQTPRLAPHREEIRKNIARLLALPADCVNLKASTEEGLGFTGRAEGIKAYAVV
TAWAGARAAFAPPSFLQRDI
>Mature_419_residues
PETSVPPRGQPVKPWAILLAAGQGHRLAAATGGLAKQFLHWQGMPLYWHAARAMSRSAAVAGIVFVFPPADYDAHAENLR
ALYAAGDLGLPCLTAVGGALRQDSVRHGLAALPADVSHVLIHDAARPFVSPALIRKICASLESGVPGAIPALPVTDTIKT
VSHDLVTGTLPRHTLRAVQTPQGFRLDLLLQAHCHAEKQKLNVTDDASLLEEMGCEIHVVEGEAANVKITNPEDMDLLRP
PHALPRPCTGMGYDVHRYAPGGRPMRLGGVLIPAGPEVAAHSDGDVLLHALTDAILGCAGLGDIGQHFSDKDPRFDNISS
ALLLDEVLDMVRKKGVQLCHADLTLIAQTPRLAPHREEIRKNIARLLALPADCVNLKASTEEGLGFTGRAEGIKAYAVVT
AWAGARAAFAPPSFLQRDI

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=149, Percent_Identity=49.6644295302013, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1789104, Length=226, Percent_Identity=33.6283185840708, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 44681; Mature: 44550

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS01295 ISPD ; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPETSVPPRGQPVKPWAILLAAGQGHRLAAATGGLAKQFLHWQGMPLYWHAARAMSRSAA
CCCCCCCCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
VAGIVFVFPPADYDAHAENLRALYAAGDLGLPCLTAVGGALRQDSVRHGLAALPADVSHV
EEEEEEEECCCCCCCHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LIHDAARPFVSPALIRKICASLESGVPGAIPALPVTDTIKTVSHDLVTGTLPRHTLRAVQ
HHHHHHCHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHC
TPQGFRLDLLLQAHCHAEKQKLNVTDDASLLEEMGCEIHVVEGEAANVKITNPEDMDLLR
CCCCCEEEEEEHHHHCHHHHCCCCCCHHHHHHHCCCEEEEEECCCCEEEECCCCCCHHCC
PPHALPRPCTGMGYDVHRYAPGGRPMRLGGVLIPAGPEVAAHSDGDVLLHALTDAILGCA
CCCCCCCCCCCCCCCCEEECCCCCEEEECCEEECCCCCCCCCCCCHHHHHHHHHHHHHHC
GLGDIGQHFSDKDPRFDNISSALLLDEVLDMVRKKGVQLCHADLTLIAQTPRLAPHREEI
CCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCCCCHHHH
RKNIARLLALPADCVNLKASTEEGLGFTGRAEGIKAYAVVTAWAGARAAFAPPSFLQRDI
HHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHCCC
>Mature Secondary Structure 
PETSVPPRGQPVKPWAILLAAGQGHRLAAATGGLAKQFLHWQGMPLYWHAARAMSRSAA
CCCCCCCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
VAGIVFVFPPADYDAHAENLRALYAAGDLGLPCLTAVGGALRQDSVRHGLAALPADVSHV
EEEEEEEECCCCCCCHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LIHDAARPFVSPALIRKICASLESGVPGAIPALPVTDTIKTVSHDLVTGTLPRHTLRAVQ
HHHHHHCHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHC
TPQGFRLDLLLQAHCHAEKQKLNVTDDASLLEEMGCEIHVVEGEAANVKITNPEDMDLLR
CCCCCEEEEEEHHHHCHHHHCCCCCCHHHHHHHCCCEEEEEECCCCEEEECCCCCCHHCC
PPHALPRPCTGMGYDVHRYAPGGRPMRLGGVLIPAGPEVAAHSDGDVLLHALTDAILGCA
CCCCCCCCCCCCCCCCEEECCCCCEEEECCEEECCCCCCCCCCCCHHHHHHHHHHHHHHC
GLGDIGQHFSDKDPRFDNISSALLLDEVLDMVRKKGVQLCHADLTLIAQTPRLAPHREEI
CCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCCCCHHHH
RKNIARLLALPADCVNLKASTEEGLGFTGRAEGIKAYAVVTAWAGARAAFAPPSFLQRDI
HHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA