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The map label for this gene is rnfE [H]

Identifier: 220904510

GI number: 220904510

Start: 1455753

End: 1456430

Strand: Direct

Name: rnfE [H]

Synonym: Ddes_1241

Alternate gene names: 220904510

Gene position: 1455753-1456430 (Clockwise)

Preceding gene: 220904509

Following gene: 220904511

Centisome position: 50.66

GC content: 55.01

Gene sequence:

>678_bases
ATGAGCATACTACAAGAATTTACCAAAGGGCTTTGGAAAGAACTGCCGCCATTCCGTCTGGTGCTGGGGCTTTGCCCCAC
CTTGGCCGTAACCAACTCTGCCGACAATGGTCTTGGCATGGGAGTGGCCGTCATTTTTGTGCTTGTACTTTCCAACATGC
TCATTTCAATGGTACGCAGCATTATCCCCAAAACAGTACGTATCGCCTGTTTTATTGTCATTGCGGCTTCACTGGTGGTC
GCGGTGGAACTGCTCATGCAGGCATATGCCTACCCTCTGTACCAGAGACTGGGCATCTTTGTGCCGCTTATCGTGGTCAA
CTGCATAATTCTCGGCCGGGCCGAAGCCTTTGCAGCCAAAAATGGCGTGGCAGCTTCCATTGCTGATGGGCTGGGCATGG
GGCTAGGTTTCACCCTGTCGCTGACCTTTCTTGGCTCGTTGCGCGAAATTTTTGGCAACGGAACATGGTTTGGACAGAGC
GTCATGTGGGAAGGCTTTGAGCCCATGCGCATCATGATTCAAGCGCCGGGGGCCTTTATTTGTCTCGGCCTCATACTGGC
GGCCATGAACATGTACAACTACTGGAAAGCCGGCAGAGGCAAGGCCGTGCCCGCCCCGCAGGGCGGTTGCGGTTCCTGCG
GGTGCTGCTCGCTGGAACGGCGTGAAGAAGCCCGGTAA

Upstream 100 bases:

>100_bases
TGGAGGCTGTGAATAAAGCCGCTCAAACGTATATGGGCCTGAAGCCTGAAATTTTCAAAGCCTGGGCCAAGTAACCCACT
GGCATACGCCTGGAGTTTCC

Downstream 100 bases:

>100_bases
CTGGGTGTTTTATACAGCCAGGAGCAGTTACGGCAAAATGCCCGACTGCAACGCCACGGCAACAAGCAGGACCTGAAAGG
CTGTCGCTGGCAGGTGTGAT

Product: electron transport complex RsxE subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MSILQEFTKGLWKELPPFRLVLGLCPTLAVTNSADNGLGMGVAVIFVLVLSNMLISMVRSIIPKTVRIACFIVIAASLVV
AVELLMQAYAYPLYQRLGIFVPLIVVNCIILGRAEAFAAKNGVAASIADGLGMGLGFTLSLTFLGSLREIFGNGTWFGQS
VMWEGFEPMRIMIQAPGAFICLGLILAAMNMYNYWKAGRGKAVPAPQGGCGSCGCCSLERREEAR

Sequences:

>Translated_225_residues
MSILQEFTKGLWKELPPFRLVLGLCPTLAVTNSADNGLGMGVAVIFVLVLSNMLISMVRSIIPKTVRIACFIVIAASLVV
AVELLMQAYAYPLYQRLGIFVPLIVVNCIILGRAEAFAAKNGVAASIADGLGMGLGFTLSLTFLGSLREIFGNGTWFGQS
VMWEGFEPMRIMIQAPGAFICLGLILAAMNMYNYWKAGRGKAVPAPQGGCGSCGCCSLERREEAR
>Mature_224_residues
SILQEFTKGLWKELPPFRLVLGLCPTLAVTNSADNGLGMGVAVIFVLVLSNMLISMVRSIIPKTVRIACFIVIAASLVVA
VELLMQAYAYPLYQRLGIFVPLIVVNCIILGRAEAFAAKNGVAASIADGLGMGLGFTLSLTFLGSLREIFGNGTWFGQSV
MWEGFEPMRIMIQAPGAFICLGLILAAMNMYNYWKAGRGKAVPAPQGGCGSCGCCSLERREEAR

Specific function: May be part of a membrane complex involved in electron transport [H]

COG id: COG4660

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfE

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family [H]

Homologues:

Organism=Escherichia coli, GI1787919, Length=201, Percent_Identity=47.2636815920398, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1787914, Length=134, Percent_Identity=31.3432835820896, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003667
- InterPro:   IPR010968 [H]

Pfam domain/function: PF02508 Rnf-Nqr [H]

EC number: NA

Molecular weight: Translated: 24149; Mature: 24018

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILQEFTKGLWKELPPFRLVLGLCPTLAVTNSADNGLGMGVAVIFVLVLSNMLISMVRS
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IIPKTVRIACFIVIAASLVVAVELLMQAYAYPLYQRLGIFVPLIVVNCIILGRAEAFAAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHC
NGVAASIADGLGMGLGFTLSLTFLGSLREIFGNGTWFGQSVMWEGFEPMRIMIQAPGAFI
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEECCHHHH
CLGLILAAMNMYNYWKAGRGKAVPAPQGGCGSCGCCSLERREEAR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCCC
>Mature Secondary Structure 
SILQEFTKGLWKELPPFRLVLGLCPTLAVTNSADNGLGMGVAVIFVLVLSNMLISMVRS
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IIPKTVRIACFIVIAASLVVAVELLMQAYAYPLYQRLGIFVPLIVVNCIILGRAEAFAAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHC
NGVAASIADGLGMGLGFTLSLTFLGSLREIFGNGTWFGQSVMWEGFEPMRIMIQAPGAFI
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEECCHHHH
CLGLILAAMNMYNYWKAGRGKAVPAPQGGCGSCGCCSLERREEAR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA