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The map label for this gene is recN [H]

Identifier: 220904486

GI number: 220904486

Start: 1429322

End: 1430917

Strand: Direct

Name: recN [H]

Synonym: Ddes_1216

Alternate gene names: 220904486

Gene position: 1429322-1430917 (Clockwise)

Preceding gene: 220904485

Following gene: 220904487

Centisome position: 49.74

GC content: 56.2

Gene sequence:

>1596_bases
ATGTTGGAATACCTTCGCATTCGCAATCTTGCTCTTATTGAAGATATGGAGCTGGAGTTCGAGCCGGGCATGAACGTTCT
CACCGGTGAAACCGGGGCCGGCAAGAGCTTTATCCTTAAGGCTCTGGGCTTTCTGCTGGGCGATAAACTGTCAGCAGATA
TGGTGCGTGGGGGAGCGGAGCGTGCTCAGGTAGAGGCCCTTTTCACCTTGAAGGATGACGATATTGTGCTGCGACGCGAA
CTGTTGGCTGAAACCGGCCGCAGCCGCCTTTATATTAACGATGAACTCCGCTCCCAGGATAGCTTGCGTGATCTGCGCCC
GCGCCTTGTGGCCCACACAAGCCAGCACGCGCAGCAAAAACTTTTGCAGCCTGCGGTCCAGGCGCGCCTGCTCGAGAGCG
GTCTTGAGCAGCCAGAGCTTCTGGCCAAGCGTGACGCCCTGCTCTCCCGCCTGACTGAAGCTGCAGCACAGCGCAAAGCG
TTGCTTGAACGGCAGGCCGGGCTTGCCGAAAAACGCGAACTGCTCGAGATGCAACAACAGGAAATTGACAAGGTCGAGCC
GGAAGATGGTGAAGAGGAACAGTTGGAGGAGGTGCGCGCCCAGGCGCGTTCACTTGAAAGCGTGCGCGAAAACTATGAGC
AGGCTCTATCGCTTTTGCACGGAAATGAAACTGAAGGATTGCTGGATCTGCTGGGTAATTTTGAAAAAGTGCTGACCCGC
ATGGCCAAAGAGGACGAATCGCTCAAGCCTGATGCTGATGCTGTCACTGCCCTGCGCCAGCAATTATCCCATCTGGGTGG
GCGCTTGCGCCGCCCACCTCAAATGGAAGACATGCCCGATATGGATCAGGTTGAGGAAAGGCTTTTCGCTTTGGCTCAAC
TTAAGCGCAAGCTGCACCGGACGCTGCCGGAAATCCTTTCCTTGCGGGATGAAATAACAGAAAATCTTTCATTTCTTGAT
GTTTGCGCTTTAGATCTGGCCCGCCTTGGGCGGGAAGAAGAGGCTCTGGTTCAGGAACTCACCGCTTTGGTGGAGACGAT
CATTCCCATTCGTCACAAGGCGGCTTTGGCCCTGGCTTCGGCCCTGGAAGAAGAATTGCGGCAACTTGGTTTTTCTGAAC
AGGTGCAAGTGATCCCACATTTTGAAAAGCAGGAAATCTGGCCCGGCGTTATGGATGAGCGCGGGCGCATCCTGTGGGCG
CCAAACCCTGGGCAACCTCCCCAACCTCTGGACAAGATTGCATCCGGAGGCGAACTTTCCCGCTTTTTACTGGCACTGGC
CAGCGTACGGCAGGATGGTGAAAACGCGATTTTTATCTTTGACGAAGTGGATGCCGGGGTGGGAGGGCTTACCCTCAACA
AGCTGGCTGAAAAGCTGTATGCGCTCGCTGCAAGCAGGCAAATGCTGCTTATCACGCACTGGCCCCAGCTGGCGGCCCGA
GCGCGCAAACATTTTCAGATCAGCAAAACCGTGCGGGATAATGCTACCTTTACCCTTTGCAGACCGCTGGACAAACAGGC
CCGGCATGCAGAGCTGGCACGCATGGCCGGGGGCGGCGCACAGGGAGAAGCCGTGGCGCACAGCCTGGAAATCTGA

Upstream 100 bases:

>100_bases
GAGAGATATTTTCGATCCCAGGCTGCGGCGCTGAACCATGCCGGGGCATCTTTATATTTAAAGTAAAACTTTAAAAACAT
ACTGTGCGGACGCAAACAAC

Downstream 100 bases:

>100_bases
CGGAACAGTTTCGACCACCCGGCACAGATACCCTGGAAAAACAGGCAGGTCTTCACGTTCGTGGAGCCATGCCGCTAAGT
ATGTATGCGTTGAGAGCAGC

Product: SMC domain-containing protein

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MLEYLRIRNLALIEDMELEFEPGMNVLTGETGAGKSFILKALGFLLGDKLSADMVRGGAERAQVEALFTLKDDDIVLRRE
LLAETGRSRLYINDELRSQDSLRDLRPRLVAHTSQHAQQKLLQPAVQARLLESGLEQPELLAKRDALLSRLTEAAAQRKA
LLERQAGLAEKRELLEMQQQEIDKVEPEDGEEEQLEEVRAQARSLESVRENYEQALSLLHGNETEGLLDLLGNFEKVLTR
MAKEDESLKPDADAVTALRQQLSHLGGRLRRPPQMEDMPDMDQVEERLFALAQLKRKLHRTLPEILSLRDEITENLSFLD
VCALDLARLGREEEALVQELTALVETIIPIRHKAALALASALEEELRQLGFSEQVQVIPHFEKQEIWPGVMDERGRILWA
PNPGQPPQPLDKIASGGELSRFLLALASVRQDGENAIFIFDEVDAGVGGLTLNKLAEKLYALAASRQMLLITHWPQLAAR
ARKHFQISKTVRDNATFTLCRPLDKQARHAELARMAGGGAQGEAVAHSLEI

Sequences:

>Translated_531_residues
MLEYLRIRNLALIEDMELEFEPGMNVLTGETGAGKSFILKALGFLLGDKLSADMVRGGAERAQVEALFTLKDDDIVLRRE
LLAETGRSRLYINDELRSQDSLRDLRPRLVAHTSQHAQQKLLQPAVQARLLESGLEQPELLAKRDALLSRLTEAAAQRKA
LLERQAGLAEKRELLEMQQQEIDKVEPEDGEEEQLEEVRAQARSLESVRENYEQALSLLHGNETEGLLDLLGNFEKVLTR
MAKEDESLKPDADAVTALRQQLSHLGGRLRRPPQMEDMPDMDQVEERLFALAQLKRKLHRTLPEILSLRDEITENLSFLD
VCALDLARLGREEEALVQELTALVETIIPIRHKAALALASALEEELRQLGFSEQVQVIPHFEKQEIWPGVMDERGRILWA
PNPGQPPQPLDKIASGGELSRFLLALASVRQDGENAIFIFDEVDAGVGGLTLNKLAEKLYALAASRQMLLITHWPQLAAR
ARKHFQISKTVRDNATFTLCRPLDKQARHAELARMAGGGAQGEAVAHSLEI
>Mature_531_residues
MLEYLRIRNLALIEDMELEFEPGMNVLTGETGAGKSFILKALGFLLGDKLSADMVRGGAERAQVEALFTLKDDDIVLRRE
LLAETGRSRLYINDELRSQDSLRDLRPRLVAHTSQHAQQKLLQPAVQARLLESGLEQPELLAKRDALLSRLTEAAAQRKA
LLERQAGLAEKRELLEMQQQEIDKVEPEDGEEEQLEEVRAQARSLESVRENYEQALSLLHGNETEGLLDLLGNFEKVLTR
MAKEDESLKPDADAVTALRQQLSHLGGRLRRPPQMEDMPDMDQVEERLFALAQLKRKLHRTLPEILSLRDEITENLSFLD
VCALDLARLGREEEALVQELTALVETIIPIRHKAALALASALEEELRQLGFSEQVQVIPHFEKQEIWPGVMDERGRILWA
PNPGQPPQPLDKIASGGELSRFLLALASVRQDGENAIFIFDEVDAGVGGLTLNKLAEKLYALAASRQMLLITHWPQLAAR
ARKHFQISKTVRDNATFTLCRPLDKQARHAELARMAGGGAQGEAVAHSLEI

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=557, Percent_Identity=32.1364452423698, Blast_Score=202, Evalue=4e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 59523; Mature: 59523

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEYLRIRNLALIEDMELEFEPGMNVLTGETGAGKSFILKALGFLLGDKLSADMVRGGAE
CCCHHHHHCHHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHCCHH
RAQVEALFTLKDDDIVLRRELLAETGRSRLYINDELRSQDSLRDLRPRLVAHTSQHAQQK
HHHHHHEEEECCCHHHHHHHHHHHCCCCEEEECHHHCCHHHHHHHHHHHHHHHHHHHHHH
LLQPAVQARLLESGLEQPELLAKRDALLSRLTEAAAQRKALLERQAGLAEKRELLEMQQQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
EIDKVEPEDGEEEQLEEVRAQARSLESVRENYEQALSLLHGNETEGLLDLLGNFEKVLTR
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
MAKEDESLKPDADAVTALRQQLSHLGGRLRRPPQMEDMPDMDQVEERLFALAQLKRKLHR
HHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TLPEILSLRDEITENLSFLDVCALDLARLGREEEALVQELTALVETIIPIRHKAALALAS
HHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALEEELRQLGFSEQVQVIPHFEKQEIWPGVMDERGRILWAPNPGQPPQPLDKIASGGELS
HHHHHHHHCCCCCCCHHCCCCCHHHCCCCCCCCCCCEEECCCCCCCCCHHHHHCCCCHHH
RFLLALASVRQDGENAIFIFDEVDAGVGGLTLNKLAEKLYALAASRQMLLITHWPQLAAR
HHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHH
ARKHFQISKTVRDNATFTLCRPLDKQARHAELARMAGGGAQGEAVAHSLEI
HHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHCCC
>Mature Secondary Structure
MLEYLRIRNLALIEDMELEFEPGMNVLTGETGAGKSFILKALGFLLGDKLSADMVRGGAE
CCCHHHHHCHHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHCCHH
RAQVEALFTLKDDDIVLRRELLAETGRSRLYINDELRSQDSLRDLRPRLVAHTSQHAQQK
HHHHHHEEEECCCHHHHHHHHHHHCCCCEEEECHHHCCHHHHHHHHHHHHHHHHHHHHHH
LLQPAVQARLLESGLEQPELLAKRDALLSRLTEAAAQRKALLERQAGLAEKRELLEMQQQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
EIDKVEPEDGEEEQLEEVRAQARSLESVRENYEQALSLLHGNETEGLLDLLGNFEKVLTR
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
MAKEDESLKPDADAVTALRQQLSHLGGRLRRPPQMEDMPDMDQVEERLFALAQLKRKLHR
HHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TLPEILSLRDEITENLSFLDVCALDLARLGREEEALVQELTALVETIIPIRHKAALALAS
HHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALEEELRQLGFSEQVQVIPHFEKQEIWPGVMDERGRILWAPNPGQPPQPLDKIASGGELS
HHHHHHHHCCCCCCCHHCCCCCHHHCCCCCCCCCCCEEECCCCCCCCCHHHHHCCCCHHH
RFLLALASVRQDGENAIFIFDEVDAGVGGLTLNKLAEKLYALAASRQMLLITHWPQLAAR
HHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHH
ARKHFQISKTVRDNATFTLCRPLDKQARHAELARMAGGGAQGEAVAHSLEI
HHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]