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The map label for this gene is cca [H]

Identifier: 220904454

GI number: 220904454

Start: 1391721

End: 1394438

Strand: Reverse

Name: cca [H]

Synonym: Ddes_1184

Alternate gene names: 220904454

Gene position: 1394438-1391721 (Counterclockwise)

Preceding gene: 220904455

Following gene: 220904453

Centisome position: 48.53

GC content: 56.07

Gene sequence:

>2718_bases
GTGAGCCATGTGAGTGCTGCCAAAGCCACGCTGATAACCTGCCATGCCAATGCGGACTTTGACGCCTTCGCCGCCATGCT
GGCCGCCCGCCACCTGTACAGCCCGCACGTGCTGCTTTTTCCGGGCAGTCAGGAACGTGGACTGCAAAAACTTTACGCCA
CTCTTGATACCAGAGCCTATAATTTTGCCGCCAGCGCGGAGCTGGACTGGGATTCATTTGACCGACTGGTACTGGTGGAT
ACGCGCCAGCGCGGCAGGGTCAGCCATGTGGCCCCGTTGCTGGATCGACCGGATGTACATGTGGAAATATGGGATCACCA
TCCGGAATCCGGCGATGACATCACCCCCGACAGGCTATATTTTGCCTCCATAGGTTCTGTCACAAGCCTTATAGTGCAGC
ACCTGCGCGAAGCCGGAATCCGCCTTGAAGCAGAAGATGCCACCCTGCTGGGACTCGGCATTTATGGCGACACCGGCTCC
TTCACTTACTCATCCACCACAACGGCGGACTTTGAAGCTGCCGCATGGCTGCTTGGTCAGGGCATGGAAGTCAACCGCAT
AAATGATCTGGCTGCCCATGAGCTGACCAGCCTGCATATACAGGCGCTCAACAGCCTTCTGGAATCGGCACAGTCCTACA
CCATTAACGGAACCCAAGTGGTTCTGGCCGAAGCCTCCATGGAGCATTATCTGGGCGACTTTGCCTATCTTGCGCACCGG
CTTATGGAAATGGAGAAATTCATTGTCCTTTTTGCCATCGGCCTCATGGGAGATCGCATACAGGTTGTGGCCCGCAGCCG
CAGCGATACTGTCAACGTGGGCGATATCTGCGCGGCTCTGGGCGGCGGCGGGCATGTCTATGCGGCCTCGGCCTCTGTGC
GGCACATGACCCTGCATGAAGTGCGCGACGCCATTCTGAGGCAGCTCTACGCACAGGCACAGCCTGACAAGACAGCGCGC
GAATACATGTCATCCCCTGCTGTAGGTGTGGAATCCACAGCGACCATACGTCAGGCAGATGAACTGATGCTGCACTTCGG
CCTCAAGGCAGTACCTGTCTTCATACCCGGTACACGCAGGTGCGAGGGGCTACTGGACGCCCAGACTGCCTCGCGCGCCA
GCGCCCACGGGCTTGGAAACGCCAGAGTGGAAGACTATATGCAACGGCGCATCCAAACCCTGCCGCCGGAAGCGGTGCTC
AAAGACCTTACCGCAGCCATTGTCGGCGCACGACAACGCCTGGTACCGATTGTCGAAAATGAAGATGTCATCGGCGTTGT
GACGCGTACCGACCTTATCAATGTTTTCGCCCACGAACCCGGAAACCTGCCCGTTCTGAAGCATTCCTCCAGCAAGGAGC
GACATGTGGGCAAGCTCATACAGGACCGTTTGCCCGCTTCTGCCAAAAACCTGCTCCATCTGGCAGGCCGGCTTGGTCGT
GACCTGGGCCTGCCCGTCTATGCTGTAGGCGGCTTTGTTCGTGATCTTCTGCTCAACAGGGCCAATCAGGATATCGACCT
GGTGGTAGAGGGCAACGGCATTGCCCTGGCCAAGGCCCTGGCCGCAGAGCTTGGTGGCAGAGTGCGCGAACATCAGGAAT
TTCTGACTTCGGTAGTCATCTTCAAAGATGCCGAAGGCCGCGAATCGCGTATTGATGTGGCTACCGCCCGGCTTGAGTAT
TATGAGTATCCCGCTGCCTTGCCTACAGTGGAGCTGTCTTCCATTAAAATGGACCTGTTCCGGCGTGATTTTTCCATAAA
TGCTTTGGCGGTGCGTCTGGACAGCACTCCATTCGGACAGCTCGTGGACTTTTTCGGCGGCCAAAGAGATATAAAAGAGC
GCATTATACGCGTACTTCATACCCTGAGCTTTGTTGAAGACCCCACACGCTGCCTGCGGGCCGTGCGTTTTGAACAGCGC
TATGATTTTCGTATAGGGGCAGGTTCAGAAAAGCTTATTAAAAATGCTATGGGGCTCAAGCTGATGGACAGGCTCAGCGG
GCCGCGGCTTTTTCATGAATTCAAGCTGATTTGTGATGAAGAGAACCCACTGGCCTGCCTTGAACGGTTGGACCAACTGG
GCATACTACCGGCCATCGCACAACAGTTGGCGCTTACGCCAGGGAAGAGAAAGATCCTCGAAGAACTTCAGGACATGCTG
TCCTGGTACCGCCTTTTATACTTCGAAAAAATGCCTCAACCCTGGCTCGTATACTTTCTCGGGCTTTGCCATGGCCTGCC
CTATGTGGAAACATCCGTACTTTACCGCCGGATGGGGCTACCTGAGAACAAAAGAAACGATATCCTTGGTCAACGGGAAC
AGATGCGCACCGTGAGAGGACGCCTGGAAAACCGACAAAAACGCCAAGGCGTGCAGGAGTTCAAGGTGAGCGTCCTTCAT
GGGCTGCTGGCCCCCCTGTCGCTGGAATCCCTGCTCTACCTTATGGCCGAAACCAACGACGAGGGGCTGCAAAAAAATCT
GTCCCGCTATATTACCCAGTGGCGTCACGAAAAAGTGGACATCACCGGCGAGGATCTCAAAAATATGGGCCTGTCGCCCG
GACCTCTTTTCAGCCAAATACTGCGCGCGGTGCTTCAGGCCAAGCTTGACGGCGAGACGCCGGGGCGGGAAGAACAACTG
GCCCTTGCACGCAGTCTGGCCCGCAACAGAACAAAAGACGAAGGAAAAAAGCTTGCAGCAGCCAAGCGCAACAATTGA

Upstream 100 bases:

>100_bases
TGCTCGGTCATGCCGACATCAGCGCCACAGAAATATATACCCATGTACAGGCAGAGCGCCTGCGTAGCATCCACCGCAAA
TTCCACCCCCGGAGCCAGAT

Downstream 100 bases:

>100_bases
TCAAGCCGGTTTTCGCGCGGGCATATCCTTGCGGACAACTCACTTTGATTGAATTTATTGCACGCATAATGTATTGAATA
TATTGCTCATATTACAATCA

Product: polynucleotide adenylyltransferase protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 905; Mature: 904

Protein sequence:

>905_residues
MSHVSAAKATLITCHANADFDAFAAMLAARHLYSPHVLLFPGSQERGLQKLYATLDTRAYNFAASAELDWDSFDRLVLVD
TRQRGRVSHVAPLLDRPDVHVEIWDHHPESGDDITPDRLYFASIGSVTSLIVQHLREAGIRLEAEDATLLGLGIYGDTGS
FTYSSTTTADFEAAAWLLGQGMEVNRINDLAAHELTSLHIQALNSLLESAQSYTINGTQVVLAEASMEHYLGDFAYLAHR
LMEMEKFIVLFAIGLMGDRIQVVARSRSDTVNVGDICAALGGGGHVYAASASVRHMTLHEVRDAILRQLYAQAQPDKTAR
EYMSSPAVGVESTATIRQADELMLHFGLKAVPVFIPGTRRCEGLLDAQTASRASAHGLGNARVEDYMQRRIQTLPPEAVL
KDLTAAIVGARQRLVPIVENEDVIGVVTRTDLINVFAHEPGNLPVLKHSSSKERHVGKLIQDRLPASAKNLLHLAGRLGR
DLGLPVYAVGGFVRDLLLNRANQDIDLVVEGNGIALAKALAAELGGRVREHQEFLTSVVIFKDAEGRESRIDVATARLEY
YEYPAALPTVELSSIKMDLFRRDFSINALAVRLDSTPFGQLVDFFGGQRDIKERIIRVLHTLSFVEDPTRCLRAVRFEQR
YDFRIGAGSEKLIKNAMGLKLMDRLSGPRLFHEFKLICDEENPLACLERLDQLGILPAIAQQLALTPGKRKILEELQDML
SWYRLLYFEKMPQPWLVYFLGLCHGLPYVETSVLYRRMGLPENKRNDILGQREQMRTVRGRLENRQKRQGVQEFKVSVLH
GLLAPLSLESLLYLMAETNDEGLQKNLSRYITQWRHEKVDITGEDLKNMGLSPGPLFSQILRAVLQAKLDGETPGREEQL
ALARSLARNRTKDEGKKLAAAKRNN

Sequences:

>Translated_905_residues
MSHVSAAKATLITCHANADFDAFAAMLAARHLYSPHVLLFPGSQERGLQKLYATLDTRAYNFAASAELDWDSFDRLVLVD
TRQRGRVSHVAPLLDRPDVHVEIWDHHPESGDDITPDRLYFASIGSVTSLIVQHLREAGIRLEAEDATLLGLGIYGDTGS
FTYSSTTTADFEAAAWLLGQGMEVNRINDLAAHELTSLHIQALNSLLESAQSYTINGTQVVLAEASMEHYLGDFAYLAHR
LMEMEKFIVLFAIGLMGDRIQVVARSRSDTVNVGDICAALGGGGHVYAASASVRHMTLHEVRDAILRQLYAQAQPDKTAR
EYMSSPAVGVESTATIRQADELMLHFGLKAVPVFIPGTRRCEGLLDAQTASRASAHGLGNARVEDYMQRRIQTLPPEAVL
KDLTAAIVGARQRLVPIVENEDVIGVVTRTDLINVFAHEPGNLPVLKHSSSKERHVGKLIQDRLPASAKNLLHLAGRLGR
DLGLPVYAVGGFVRDLLLNRANQDIDLVVEGNGIALAKALAAELGGRVREHQEFLTSVVIFKDAEGRESRIDVATARLEY
YEYPAALPTVELSSIKMDLFRRDFSINALAVRLDSTPFGQLVDFFGGQRDIKERIIRVLHTLSFVEDPTRCLRAVRFEQR
YDFRIGAGSEKLIKNAMGLKLMDRLSGPRLFHEFKLICDEENPLACLERLDQLGILPAIAQQLALTPGKRKILEELQDML
SWYRLLYFEKMPQPWLVYFLGLCHGLPYVETSVLYRRMGLPENKRNDILGQREQMRTVRGRLENRQKRQGVQEFKVSVLH
GLLAPLSLESLLYLMAETNDEGLQKNLSRYITQWRHEKVDITGEDLKNMGLSPGPLFSQILRAVLQAKLDGETPGREEQL
ALARSLARNRTKDEGKKLAAAKRNN
>Mature_904_residues
SHVSAAKATLITCHANADFDAFAAMLAARHLYSPHVLLFPGSQERGLQKLYATLDTRAYNFAASAELDWDSFDRLVLVDT
RQRGRVSHVAPLLDRPDVHVEIWDHHPESGDDITPDRLYFASIGSVTSLIVQHLREAGIRLEAEDATLLGLGIYGDTGSF
TYSSTTTADFEAAAWLLGQGMEVNRINDLAAHELTSLHIQALNSLLESAQSYTINGTQVVLAEASMEHYLGDFAYLAHRL
MEMEKFIVLFAIGLMGDRIQVVARSRSDTVNVGDICAALGGGGHVYAASASVRHMTLHEVRDAILRQLYAQAQPDKTARE
YMSSPAVGVESTATIRQADELMLHFGLKAVPVFIPGTRRCEGLLDAQTASRASAHGLGNARVEDYMQRRIQTLPPEAVLK
DLTAAIVGARQRLVPIVENEDVIGVVTRTDLINVFAHEPGNLPVLKHSSSKERHVGKLIQDRLPASAKNLLHLAGRLGRD
LGLPVYAVGGFVRDLLLNRANQDIDLVVEGNGIALAKALAAELGGRVREHQEFLTSVVIFKDAEGRESRIDVATARLEYY
EYPAALPTVELSSIKMDLFRRDFSINALAVRLDSTPFGQLVDFFGGQRDIKERIIRVLHTLSFVEDPTRCLRAVRFEQRY
DFRIGAGSEKLIKNAMGLKLMDRLSGPRLFHEFKLICDEENPLACLERLDQLGILPAIAQQLALTPGKRKILEELQDMLS
WYRLLYFEKMPQPWLVYFLGLCHGLPYVETSVLYRRMGLPENKRNDILGQREQMRTVRGRLENRQKRQGVQEFKVSVLHG
LLAPLSLESLLYLMAETNDEGLQKNLSRYITQWRHEKVDITGEDLKNMGLSPGPLFSQILRAVLQAKLDGETPGREEQLA
LARSLARNRTKDEGKKLAAAKRNN

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=270, Percent_Identity=29.6296296296296, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 100863; Mature: 100732

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHVSAAKATLITCHANADFDAFAAMLAARHLYSPHVLLFPGSQERGLQKLYATLDTRAY
CCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHC
NFAASAELDWDSFDRLVLVDTRQRGRVSHVAPLLDRPDVHVEIWDHHPESGDDITPDRLY
CEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHH
FASIGSVTSLIVQHLREAGIRLEAEDATLLGLGIYGDTGSFTYSSTTTADFEAAAWLLGQ
HHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEEECCCCCEEECCCCCCCHHHHHHHHCC
GMEVNRINDLAAHELTSLHIQALNSLLESAQSYTINGTQVVLAEASMEHYLGDFAYLAHR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCEEEEEHHHHHHHHHHHHHHHHH
LMEMEKFIVLFAIGLMGDRIQVVARSRSDTVNVGDICAALGGGGHVYAASASVRHMTLHE
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHH
VRDAILRQLYAQAQPDKTAREYMSSPAVGVESTATIRQADELMLHFGLKAVPVFIPGTRR
HHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHH
CEGLLDAQTASRASAHGLGNARVEDYMQRRIQTLPPEAVLKDLTAAIVGARQRLVPIVEN
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEECC
EDVIGVVTRTDLINVFAHEPGNLPVLKHSSSKERHVGKLIQDRLPASAKNLLHLAGRLGR
CCEEEEEEHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
DLGLPVYAVGGFVRDLLLNRANQDIDLVVEGNGIALAKALAAELGGRVREHQEFLTSVVI
CCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHHE
FKDAEGRESRIDVATARLEYYEYPAALPTVELSSIKMDLFRRDFSINALAVRLDSTPFGQ
EECCCCCCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCEEEEEEECCCCHHH
LVDFFGGQRDIKERIIRVLHTLSFVEDPTRCLRAVRFEQRYDFRIGAGSEKLIKNAMGLK
HHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHH
LMDRLSGPRLFHEFKLICDEENPLACLERLDQLGILPAIAQQLALTPGKRKILEELQDML
HHHHCCCCHHHHHHHHEECCCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHH
SWYRLLYFEKMPQPWLVYFLGLCHGLPYVETSVLYRRMGLPENKRNDILGQREQMRTVRG
HHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHH
RLENRQKRQGVQEFKVSVLHGLLAPLSLESLLYLMAETNDEGLQKNLSRYITQWRHEKVD
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
ITGEDLKNMGLSPGPLFSQILRAVLQAKLDGETPGREEQLALARSLARNRTKDEGKKLAA
CCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
AKRNN
HCCCC
>Mature Secondary Structure 
SHVSAAKATLITCHANADFDAFAAMLAARHLYSPHVLLFPGSQERGLQKLYATLDTRAY
CCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHC
NFAASAELDWDSFDRLVLVDTRQRGRVSHVAPLLDRPDVHVEIWDHHPESGDDITPDRLY
CEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHH
FASIGSVTSLIVQHLREAGIRLEAEDATLLGLGIYGDTGSFTYSSTTTADFEAAAWLLGQ
HHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEEECCCCCEEECCCCCCCHHHHHHHHCC
GMEVNRINDLAAHELTSLHIQALNSLLESAQSYTINGTQVVLAEASMEHYLGDFAYLAHR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCEEEEEHHHHHHHHHHHHHHHHH
LMEMEKFIVLFAIGLMGDRIQVVARSRSDTVNVGDICAALGGGGHVYAASASVRHMTLHE
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHH
VRDAILRQLYAQAQPDKTAREYMSSPAVGVESTATIRQADELMLHFGLKAVPVFIPGTRR
HHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHH
CEGLLDAQTASRASAHGLGNARVEDYMQRRIQTLPPEAVLKDLTAAIVGARQRLVPIVEN
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEECC
EDVIGVVTRTDLINVFAHEPGNLPVLKHSSSKERHVGKLIQDRLPASAKNLLHLAGRLGR
CCEEEEEEHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
DLGLPVYAVGGFVRDLLLNRANQDIDLVVEGNGIALAKALAAELGGRVREHQEFLTSVVI
CCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHHE
FKDAEGRESRIDVATARLEYYEYPAALPTVELSSIKMDLFRRDFSINALAVRLDSTPFGQ
EECCCCCCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCEEEEEEECCCCHHH
LVDFFGGQRDIKERIIRVLHTLSFVEDPTRCLRAVRFEQRYDFRIGAGSEKLIKNAMGLK
HHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHH
LMDRLSGPRLFHEFKLICDEENPLACLERLDQLGILPAIAQQLALTPGKRKILEELQDML
HHHHCCCCHHHHHHHHEECCCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHH
SWYRLLYFEKMPQPWLVYFLGLCHGLPYVETSVLYRRMGLPENKRNDILGQREQMRTVRG
HHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHH
RLENRQKRQGVQEFKVSVLHGLLAPLSLESLLYLMAETNDEGLQKNLSRYITQWRHEKVD
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
ITGEDLKNMGLSPGPLFSQILRAVLQAKLDGETPGREEQLALARSLARNRTKDEGKKLAA
CCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
AKRNN
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA