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The map label for this gene is ksgA [H]

Identifier: 220904363

GI number: 220904363

Start: 1278725

End: 1279564

Strand: Reverse

Name: ksgA [H]

Synonym: Ddes_1091

Alternate gene names: 220904363

Gene position: 1279564-1278725 (Counterclockwise)

Preceding gene: 220904364

Following gene: 220904362

Centisome position: 44.53

GC content: 60.0

Gene sequence:

>840_bases
ATGCCTGCCGCATTCCCCCATATGAAAAAAAATATTGTCACACATACCCCACGGGCCAAAAAAAGTCTGGGCCAGCATTT
TTTACGGCGCGAAGACACCTGCTGTCGTATTGCGGCGCTCATTGCGCCAGCCGCCCAAGATCGCATCATTGAAATCGGCC
CCGGACCCGGCGCGTTGACCCGCGCCATTGAAGCCACCCCCCATTCCCGGCTGCTACTGCTGGAAAAAGACCGCCACTGG
GCCGCCGAACGGCAGCGTCTGGGCGGTGCCAACACCCAGGCCGTACTCATTGATGCCCTGAAGTTTGCATGGCGGCGCAT
CAGCCCTGAAGAGCCGTGGAAAATCATAGGCAACCTGCCCTACAATGTGGCTTCTCCCCTGATCTGGGACATTTTCTCCC
GGTCCACAGGCTGGCAGCGGGCAGCTTTTATGGTGCAGAAGGAAGTGGGCCAGCGGCTGGCGGCCCATCCCGGTACAGGA
CATTACGGTGCGCTTTCGGTATGGGTGCAAAGTTACGCCCGCCCCCGCATGGAATTCGTGGTGGGTCCAGGCGCGTTCAG
CCCGCCCCCCAAGGTAGATTCCGCGGTTCTGAGCTTTGAGCCATTGCCCGCCAATGAACGCCCCGCACATCCGGAACTTC
TCTCGCGTCTTCTGCGGATATGCTTTCAGCAGCGTCGCAAACAGCTTGGCGGCGTATTCCGCCGCGCCGCGCAGCCGGGC
CTTGAAAAATCCCTGCACGATGCCTGCATTGACCCGGCCTTGCGCCCCGAAGCCCTGACGTGCTCAGATTTCCAGCGTAT
AGCCGCTTTTTGGGCTTCACAGCTTGACAACAGGGCATAA

Upstream 100 bases:

>100_bases
GCGCCGCCTGTTCGGTTTTTCCACCTCCTTGCGCTCGGCCACACAGGGCCGGGCCGGACTGGTGATGACCTTTGACCGTT
TTGACCTGCCCTGAGGCCCC

Downstream 100 bases:

>100_bases
AAAGTTTTCAGTCTATCCATAATCTTGCTGCGTTGTATGCCTTCCGGCTTTTTCATCGCCAGGAAGGCCGCCTGAAACAA
CGCGGGGGAACCTTTCAGGA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MPAAFPHMKKNIVTHTPRAKKSLGQHFLRREDTCCRIAALIAPAAQDRIIEIGPGPGALTRAIEATPHSRLLLLEKDRHW
AAERQRLGGANTQAVLIDALKFAWRRISPEEPWKIIGNLPYNVASPLIWDIFSRSTGWQRAAFMVQKEVGQRLAAHPGTG
HYGALSVWVQSYARPRMEFVVGPGAFSPPPKVDSAVLSFEPLPANERPAHPELLSRLLRICFQQRRKQLGGVFRRAAQPG
LEKSLHDACIDPALRPEALTCSDFQRIAAFWASQLDNRA

Sequences:

>Translated_279_residues
MPAAFPHMKKNIVTHTPRAKKSLGQHFLRREDTCCRIAALIAPAAQDRIIEIGPGPGALTRAIEATPHSRLLLLEKDRHW
AAERQRLGGANTQAVLIDALKFAWRRISPEEPWKIIGNLPYNVASPLIWDIFSRSTGWQRAAFMVQKEVGQRLAAHPGTG
HYGALSVWVQSYARPRMEFVVGPGAFSPPPKVDSAVLSFEPLPANERPAHPELLSRLLRICFQQRRKQLGGVFRRAAQPG
LEKSLHDACIDPALRPEALTCSDFQRIAAFWASQLDNRA
>Mature_278_residues
PAAFPHMKKNIVTHTPRAKKSLGQHFLRREDTCCRIAALIAPAAQDRIIEIGPGPGALTRAIEATPHSRLLLLEKDRHWA
AERQRLGGANTQAVLIDALKFAWRRISPEEPWKIIGNLPYNVASPLIWDIFSRSTGWQRAAFMVQKEVGQRLAAHPGTGH
YGALSVWVQSYARPRMEFVVGPGAFSPPPKVDSAVLSFEPLPANERPAHPELLSRLLRICFQQRRKQLGGVFRRAAQPGL
EKSLHDACIDPALRPEALTCSDFQRIAAFWASQLDNRA

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=305, Percent_Identity=33.4426229508197, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI7657198, Length=254, Percent_Identity=29.9212598425197, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1786236, Length=265, Percent_Identity=34.3396226415094, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25141369, Length=301, Percent_Identity=30.2325581395349, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI25146882, Length=220, Percent_Identity=32.7272727272727, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6324989, Length=226, Percent_Identity=31.4159292035398, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21357273, Length=300, Percent_Identity=30.6666666666667, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21358017, Length=276, Percent_Identity=30.7971014492754, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 31118; Mature: 30987

Theoretical pI: Translated: 10.74; Mature: 10.74

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAAFPHMKKNIVTHTPRAKKSLGQHFLRREDTCCRIAALIAPAAQDRIIEIGPGPGALT
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCHHHH
RAIEATPHSRLLLLEKDRHWAAERQRLGGANTQAVLIDALKFAWRRISPEEPWKIIGNLP
HHHHCCCCCEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCC
YNVASPLIWDIFSRSTGWQRAAFMVQKEVGQRLAAHPGTGHYGALSVWVQSYARPRMEFV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEE
VGPGAFSPPPKVDSAVLSFEPLPANERPAHPELLSRLLRICFQQRRKQLGGVFRRAAQPG
ECCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LEKSLHDACIDPALRPEALTCSDFQRIAAFWASQLDNRA
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PAAFPHMKKNIVTHTPRAKKSLGQHFLRREDTCCRIAALIAPAAQDRIIEIGPGPGALT
CCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCCHHHH
RAIEATPHSRLLLLEKDRHWAAERQRLGGANTQAVLIDALKFAWRRISPEEPWKIIGNLP
HHHHCCCCCEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCC
YNVASPLIWDIFSRSTGWQRAAFMVQKEVGQRLAAHPGTGHYGALSVWVQSYARPRMEFV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEE
VGPGAFSPPPKVDSAVLSFEPLPANERPAHPELLSRLLRICFQQRRKQLGGVFRRAAQPG
ECCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LEKSLHDACIDPALRPEALTCSDFQRIAAFWASQLDNRA
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA