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The map label for this gene is hycF [C]

Identifier: 220904268

GI number: 220904268

Start: 1163684

End: 1164784

Strand: Reverse

Name: hycF [C]

Synonym: Ddes_0995

Alternate gene names: 220904268

Gene position: 1164784-1163684 (Counterclockwise)

Preceding gene: 220904272

Following gene: 220904267

Centisome position: 40.54

GC content: 56.95

Gene sequence:

>1101_bases
ATGCCTGCCAAAGTATATTTTACCGATACACATTCCCGCTCACATGAAGAAAGCAAGATGGTCAAGGTCGCCCGCCTGTG
CGATGCCCTTAACCTGAAAAAAATTATCAAAAAAAATGAACTGACTGCCGTCAAGCTGCATTTTGGAGAATACGGCAACG
ACACGCACCTGAACCCCACCCTGGTGCGGCAGGTGGTGGACAAGATCACCGCCGCCGGGGGCAAACCCTTTCTGACGGAC
ACCACTACCCTGTACTCCGGCAGCAGACATAATGCGGTCGACCACCTGCAGACCGCCTATCTCAACGGCTTTGCTCCTTC
GGTGGTGGGCGCTCCCGTTATTATTGCCGATGGCCTGTTCGGCGGCAACGACGCGCCGGTGCGCATCAACTGCAAACACT
TCAAGGAAGTTCATATCGCCACTGAAATACGCAACGCGCCGGCTATGGTGGTACTCAGCCATTTCAAGGGGCATCAGATG
GCTGGCTTTGGCGGAGCCGTCAAAAATCTGGCAATGGGAGGTGCATCTGTACGGGGTAAGCGGGAGCAGCACGCCACCCA
TGTGAGCATCAACAAAGACGACTGCATCGGCTGCGCCAAATGCGTGCATTCATGCCCGCAGCAGGCCCTGAGCATGCGCG
ACCATAAAAGCCATGTGGAAACGGCGCGATGTATCGGCTGCTTCGAATGCATGACAGTCTGCCCGGCCAAGGCCATTGTT
ATTGACTGGGCGACGGAAATGGAACCGTTTATGGAGCGCATGACCGAGTATGCCTATGGTACGGTCAAGGGCAGAAGCAG
GCGCGTCTGCTATATCAATTTTGTGCTCAACGTAACGCCGGACTGCGACTGCGTGGCCTGGAGCGACATGCCGCTGGTGC
CTGATGTGGGCATTCTGGCTTCCACAGATCCCGTAGCCCTGGACCAGGCGTGTCTTGACCTGGTCAGCAGCGCCCCCAGC
CTGTGCGGCAGCGCGCAGGAAAAAAACGGGGAACAGGACAAGTTCACCGCGCGCTGGCCCCATACACGCGGGGGCGTTCA
ACTGCAGTATGGCGAAGAAATCGGGCTGGGCAGCCGGGCCTACGAACTCATAAAACTGTAA

Upstream 100 bases:

>100_bases
TTTTTCATGATTCCGCCTAGATTTTTACTTGCGAAAACCCCTGGCTGCGGCATAAAAAACCTTATGGTTACAGCATAAAA
AGCCAGCTATGGAGGACAGC

Downstream 100 bases:

>100_bases
TTTCCGCCGCCAGGTCGCGCTTCGCGCCGCCTGTAACGGGTCCAAGCCTGTAAAACATTGCAGACTGCTGCAAAAGCTGA
AGCCGGAATTGCCTGTATGC

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: None

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MPAKVYFTDTHSRSHEESKMVKVARLCDALNLKKIIKKNELTAVKLHFGEYGNDTHLNPTLVRQVVDKITAAGGKPFLTD
TTTLYSGSRHNAVDHLQTAYLNGFAPSVVGAPVIIADGLFGGNDAPVRINCKHFKEVHIATEIRNAPAMVVLSHFKGHQM
AGFGGAVKNLAMGGASVRGKREQHATHVSINKDDCIGCAKCVHSCPQQALSMRDHKSHVETARCIGCFECMTVCPAKAIV
IDWATEMEPFMERMTEYAYGTVKGRSRRVCYINFVLNVTPDCDCVAWSDMPLVPDVGILASTDPVALDQACLDLVSSAPS
LCGSAQEKNGEQDKFTARWPHTRGGVQLQYGEEIGLGSRAYELIKL

Sequences:

>Translated_366_residues
MPAKVYFTDTHSRSHEESKMVKVARLCDALNLKKIIKKNELTAVKLHFGEYGNDTHLNPTLVRQVVDKITAAGGKPFLTD
TTTLYSGSRHNAVDHLQTAYLNGFAPSVVGAPVIIADGLFGGNDAPVRINCKHFKEVHIATEIRNAPAMVVLSHFKGHQM
AGFGGAVKNLAMGGASVRGKREQHATHVSINKDDCIGCAKCVHSCPQQALSMRDHKSHVETARCIGCFECMTVCPAKAIV
IDWATEMEPFMERMTEYAYGTVKGRSRRVCYINFVLNVTPDCDCVAWSDMPLVPDVGILASTDPVALDQACLDLVSSAPS
LCGSAQEKNGEQDKFTARWPHTRGGVQLQYGEEIGLGSRAYELIKL
>Mature_365_residues
PAKVYFTDTHSRSHEESKMVKVARLCDALNLKKIIKKNELTAVKLHFGEYGNDTHLNPTLVRQVVDKITAAGGKPFLTDT
TTLYSGSRHNAVDHLQTAYLNGFAPSVVGAPVIIADGLFGGNDAPVRINCKHFKEVHIATEIRNAPAMVVLSHFKGHQMA
GFGGAVKNLAMGGASVRGKREQHATHVSINKDDCIGCAKCVHSCPQQALSMRDHKSHVETARCIGCFECMTVCPAKAIVI
DWATEMEPFMERMTEYAYGTVKGRSRRVCYINFVLNVTPDCDCVAWSDMPLVPDVGILASTDPVALDQACLDLVSSAPSL
CGSAQEKNGEQDKFTARWPHTRGGVQLQYGEEIGLGSRAYELIKL

Specific function: Probable Electron Transfer Protein For Hydrogenase 3 [C]

COG id: COG2768

COG function: function code R; Uncharacterized Fe-S center protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 39932; Mature: 39801

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAKVYFTDTHSRSHEESKMVKVARLCDALNLKKIIKKNELTAVKLHFGEYGNDTHLNPT
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHH
LVRQVVDKITAAGGKPFLTDTTTLYSGSRHNAVDHLQTAYLNGFAPSVVGAPVIIADGLF
HHHHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCHHHHCCCEEEECCCC
GGNDAPVRINCKHFKEVHIATEIRNAPAMVVLSHFKGHQMAGFGGAVKNLAMGGASVRGK
CCCCCCEEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCC
REQHATHVSINKDDCIGCAKCVHSCPQQALSMRDHKSHVETARCIGCFECMTVCPAKAIV
HHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IDWATEMEPFMERMTEYAYGTVKGRSRRVCYINFVLNVTPDCDCVAWSDMPLVPDVGILA
EECHHHHHHHHHHHHHHHCEEECCCCCEEEEEEEEEECCCCCCEEEECCCCCCCCCCCEE
STDPVALDQACLDLVSSAPSLCGSAQEKNGEQDKFTARWPHTRGGVQLQYGEEIGLGSRA
CCCCHHHHHHHHHHHHCCHHHHCCHHHCCCCCCCEEEECCCCCCCEEEEECCCCCCCHHH
YELIKL
HHHHCC
>Mature Secondary Structure 
PAKVYFTDTHSRSHEESKMVKVARLCDALNLKKIIKKNELTAVKLHFGEYGNDTHLNPT
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHH
LVRQVVDKITAAGGKPFLTDTTTLYSGSRHNAVDHLQTAYLNGFAPSVVGAPVIIADGLF
HHHHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCHHHHCCCEEEECCCC
GGNDAPVRINCKHFKEVHIATEIRNAPAMVVLSHFKGHQMAGFGGAVKNLAMGGASVRGK
CCCCCCEEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCC
REQHATHVSINKDDCIGCAKCVHSCPQQALSMRDHKSHVETARCIGCFECMTVCPAKAIV
HHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IDWATEMEPFMERMTEYAYGTVKGRSRRVCYINFVLNVTPDCDCVAWSDMPLVPDVGILA
EECHHHHHHHHHHHHHHHCEEECCCCCEEEEEEEEEECCCCCCEEEECCCCCCCCCCCEE
STDPVALDQACLDLVSSAPSLCGSAQEKNGEQDKFTARWPHTRGGVQLQYGEEIGLGSRA
CCCCHHHHHHHHHHHHCCHHHHCCHHHCCCCCCCEEEECCCCCCCEEEEECCCCCCCHHH
YELIKL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA