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The map label for this gene is btuB [H]

Identifier: 220904266

GI number: 220904266

Start: 1160436

End: 1162643

Strand: Reverse

Name: btuB [H]

Synonym: Ddes_0993

Alternate gene names: 220904266

Gene position: 1162643-1160436 (Counterclockwise)

Preceding gene: 220904267

Following gene: 220904265

Centisome position: 40.46

GC content: 55.03

Gene sequence:

>2208_bases
ATGAAACTCATCCTTTGCCTGTGCCTGCTGGCTGTGACGGCCTTTCCCCAGGCAGGCCACGCAGCCGGTTCCGACAATCT
CATGCAGGACGTCTTTGTTCTGGACCCGGTAAAAGTCACCGGGCGTATGGAAGAAGACAGGCTGGACAGAACCACCGCCA
TCGTGGTGGAAAAAAACCGCAGCAACAACGTGGCCGACTACCTGGTGCGCGACCCTGAAATTTCTTTCAAGCGCAAGGCC
GCCATGGGAGACAGCAGCGACATCATTTCCATACGCGGCATGGAATCCAAAAGGATCATGCTCAACCTTGACGGCCGCAA
CATCAGTTCTACCGGCATGTCCGGCGGCAACTATATCGACTTCGGCACCATTCCACTGGACAATATCGAGCGGATCGAAG
TGATCAAAGGCGGCAGTTCGGTTGAATACGGCAACAACGCCCTGGGCGGCGTCATCAATGCCTATACCCGCCGCCCCACA
GAGCAGCCCTACTTCAGCGTTTACGCCACCATGGGCGGCTGGAAGGATTTTTATGACTTTCATAACGTGCGCGGCAGCTA
TGCCCAGAAATTCAACGCCATCGGCCTGAGTGTGGGGTTGAGCCATCAGCACGCCGACCCTTTTTTTCGCAACAACGACT
ATGATTCCTTTCACTTCAATCCCAAACTGTACGTAGACCTGCCCTGGCGGGCCGAGCTTACCCTGGGCTACAATTACAGC
CAGACCCGGCGCGGGCTTATCCGCACCAACAGAAACGACGGCAACCCCACCAGCGACTCCAATCCGGACCTGCCCGGCTT
TGATTCTCCCATAGACTCCAACTATCCCACAGCCAGCGGCGAAAGCTTTGCCGGGGGATCCCCCACGCCCTCGATGACCG
TTATTGGCGACGGCGCGCACTGGACCAAATACCGCCATCTGATGGACTTCACCTACCGGCAGGAATTTCTGGAGACAGCC
TACTTTGAGCTTTCCGGCTTCAAGAACAGCGAAAATCGCCGTGAAAAAAACTATGCGGATATTGACGCCCGCATGCAGAT
TAAAAAAGGCCCGGACGGCTTCGATCCGTCCAAAACAAAAAACGGCGATCTCGTCTTGGACCGCAATGTCATTGTAGACA
ATACTCACGGCTTCAAGTTCAAGACAGGAGCCACCATTCACGGCCACGAGCTTATGACCGGCGTGGAGTACAAAAGACTG
ACCCCCGGTCCCATTACAGTGGAATATGTGGATAAAAACTACAACAAGGCCGGACCAAACAAGTGGACGGGAAAAATGGA
ATCCTCCGAAGCGGGCGCGCCGGCCACGGTAGTGGGGGCGTTTCTGGCCGACAAGTTTTCCCTTACCGACAGCCTGCATC
TGGATATCGGCCTGCGCTACGACAGCTTCAGCTACAGCCCCGACGGCCAGGACAGAAACTATTTCGGCTCGCAGCTTTCC
CCCAAGGCCATGATCACCTACGAGTTTACCGAAAACCAGTCCGCTTCCCTGGCCCTGTACCAGAACTACAGAACCCCCAC
CATTCCCGAACTGTACTGGAATTCGCAGTCATCATCACGGGACAATACCGTCAATGTACCCTATCTTGTGGGCAAGGACA
TAAAACCAGAAACGGCCCGCGGCATTGACGCCGCCTACAAATACAGCTTCGGCGGCAAGGGTTTTCTCAAGCTTTCCGGC
TTTTACTACAATATTCAGGATTATATCGTCCACAAGCCCGTCTACGTAAACAGGCCGCAAAGCTATCAGGCATGGGCCGC
CTATAATATTGACGCCGAAATATACGGCGCTACGCTGAGCGGCAGCTATGACGTGCTGGACAATGTTACGGTAAAGGCCG
CCGTGACCAGACAGGACAGCAAAAAGTCCAATGACCCCGCCGACCCCGGGGGAGTGATGGAAAAGCTGGAGTATATTCCC
GACTGGAAAGGAACCCTGGGAACCACCTGGAAAATCAATGAGCAGTTCACTCTTGACACTGTGCTGACCTATGTGGGCGA
GCGCAGTTATTACGTCAACACGGCCCAACTGCGTAAGGGCACCCTGCACGACTACGCCACCCTGGGTGCAAGCCTCAGCT
ACAAGGTGGACGAACATCTGACCCTTGAAGCCTATGCGGACAACATCACCAACACCCAGTATGAAGAAGCCTGGGGGTAC
CCGGCACTGGGATTCAACGCGGGAGTCAGCATCAAATGGGAACTGTAG

Upstream 100 bases:

>100_bases
AGCAGCCTTTTTTTCCAGCAATTGATAGCACTTTTTTTAAAAACATCACATGCGCCGGCCTGACACCAACCTTCAGCACG
GGCGCTGCCAGAGGGTATGT

Downstream 100 bases:

>100_bases
ACGAAAAGCAGCAGAAGCAGCGTGAATACTGGAACGCCCGGTCACGCACCTTTCCGCGCTTTGAAGAAGGCGACGACAAT
TACGAAGCGGGCATGCTGCG

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 735; Mature: 735

Protein sequence:

>735_residues
MKLILCLCLLAVTAFPQAGHAAGSDNLMQDVFVLDPVKVTGRMEEDRLDRTTAIVVEKNRSNNVADYLVRDPEISFKRKA
AMGDSSDIISIRGMESKRIMLNLDGRNISSTGMSGGNYIDFGTIPLDNIERIEVIKGGSSVEYGNNALGGVINAYTRRPT
EQPYFSVYATMGGWKDFYDFHNVRGSYAQKFNAIGLSVGLSHQHADPFFRNNDYDSFHFNPKLYVDLPWRAELTLGYNYS
QTRRGLIRTNRNDGNPTSDSNPDLPGFDSPIDSNYPTASGESFAGGSPTPSMTVIGDGAHWTKYRHLMDFTYRQEFLETA
YFELSGFKNSENRREKNYADIDARMQIKKGPDGFDPSKTKNGDLVLDRNVIVDNTHGFKFKTGATIHGHELMTGVEYKRL
TPGPITVEYVDKNYNKAGPNKWTGKMESSEAGAPATVVGAFLADKFSLTDSLHLDIGLRYDSFSYSPDGQDRNYFGSQLS
PKAMITYEFTENQSASLALYQNYRTPTIPELYWNSQSSSRDNTVNVPYLVGKDIKPETARGIDAAYKYSFGGKGFLKLSG
FYYNIQDYIVHKPVYVNRPQSYQAWAAYNIDAEIYGATLSGSYDVLDNVTVKAAVTRQDSKKSNDPADPGGVMEKLEYIP
DWKGTLGTTWKINEQFTLDTVLTYVGERSYYVNTAQLRKGTLHDYATLGASLSYKVDEHLTLEAYADNITNTQYEEAWGY
PALGFNAGVSIKWEL

Sequences:

>Translated_735_residues
MKLILCLCLLAVTAFPQAGHAAGSDNLMQDVFVLDPVKVTGRMEEDRLDRTTAIVVEKNRSNNVADYLVRDPEISFKRKA
AMGDSSDIISIRGMESKRIMLNLDGRNISSTGMSGGNYIDFGTIPLDNIERIEVIKGGSSVEYGNNALGGVINAYTRRPT
EQPYFSVYATMGGWKDFYDFHNVRGSYAQKFNAIGLSVGLSHQHADPFFRNNDYDSFHFNPKLYVDLPWRAELTLGYNYS
QTRRGLIRTNRNDGNPTSDSNPDLPGFDSPIDSNYPTASGESFAGGSPTPSMTVIGDGAHWTKYRHLMDFTYRQEFLETA
YFELSGFKNSENRREKNYADIDARMQIKKGPDGFDPSKTKNGDLVLDRNVIVDNTHGFKFKTGATIHGHELMTGVEYKRL
TPGPITVEYVDKNYNKAGPNKWTGKMESSEAGAPATVVGAFLADKFSLTDSLHLDIGLRYDSFSYSPDGQDRNYFGSQLS
PKAMITYEFTENQSASLALYQNYRTPTIPELYWNSQSSSRDNTVNVPYLVGKDIKPETARGIDAAYKYSFGGKGFLKLSG
FYYNIQDYIVHKPVYVNRPQSYQAWAAYNIDAEIYGATLSGSYDVLDNVTVKAAVTRQDSKKSNDPADPGGVMEKLEYIP
DWKGTLGTTWKINEQFTLDTVLTYVGERSYYVNTAQLRKGTLHDYATLGASLSYKVDEHLTLEAYADNITNTQYEEAWGY
PALGFNAGVSIKWEL
>Mature_735_residues
MKLILCLCLLAVTAFPQAGHAAGSDNLMQDVFVLDPVKVTGRMEEDRLDRTTAIVVEKNRSNNVADYLVRDPEISFKRKA
AMGDSSDIISIRGMESKRIMLNLDGRNISSTGMSGGNYIDFGTIPLDNIERIEVIKGGSSVEYGNNALGGVINAYTRRPT
EQPYFSVYATMGGWKDFYDFHNVRGSYAQKFNAIGLSVGLSHQHADPFFRNNDYDSFHFNPKLYVDLPWRAELTLGYNYS
QTRRGLIRTNRNDGNPTSDSNPDLPGFDSPIDSNYPTASGESFAGGSPTPSMTVIGDGAHWTKYRHLMDFTYRQEFLETA
YFELSGFKNSENRREKNYADIDARMQIKKGPDGFDPSKTKNGDLVLDRNVIVDNTHGFKFKTGATIHGHELMTGVEYKRL
TPGPITVEYVDKNYNKAGPNKWTGKMESSEAGAPATVVGAFLADKFSLTDSLHLDIGLRYDSFSYSPDGQDRNYFGSQLS
PKAMITYEFTENQSASLALYQNYRTPTIPELYWNSQSSSRDNTVNVPYLVGKDIKPETARGIDAAYKYSFGGKGFLKLSG
FYYNIQDYIVHKPVYVNRPQSYQAWAAYNIDAEIYGATLSGSYDVLDNVTVKAAVTRQDSKKSNDPADPGGVMEKLEYIP
DWKGTLGTTWKINEQFTLDTVLTYVGERSYYVNTAQLRKGTLHDYATLGASLSYKVDEHLTLEAYADNITNTQYEEAWGY
PALGFNAGVSIKWEL

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=164, Percent_Identity=29.2682926829268, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1788478, Length=168, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 82202; Mature: 82202

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLILCLCLLAVTAFPQAGHAAGSDNLMQDVFVLDPVKVTGRMEEDRLDRTTAIVVEKNR
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEEEECCCCEECCCCHHHCCCEEEEEEECCC
SNNVADYLVRDPEISFKRKAAMGDSSDIISIRGMESKRIMLNLDGRNISSTGMSGGNYID
CCCCHHHEEECCCCCCHHHHCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEE
FGTIPLDNIERIEVIKGGSSVEYGNNALGGVINAYTRRPTEQPYFSVYATMGGWKDFYDF
ECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHH
HNVRGSYAQKFNAIGLSVGLSHQHADPFFRNNDYDSFHFNPKLYVDLPWRAELTLGYNYS
HHCCCHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCEEECCEEEEECCCEEEEEEECCHH
QTRRGLIRTNRNDGNPTSDSNPDLPGFDSPIDSNYPTASGESFAGGSPTPSMTVIGDGAH
HHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCH
WTKYRHLMDFTYRQEFLETAYFELSGFKNSENRREKNYADIDARMQIKKGPDGFDPSKTK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCEEEEEECCCCCCCCCCCC
NGDLVLDRNVIVDNTHGFKFKTGATIHGHELMTGVEYKRLTPGPITVEYVDKNYNKAGPN
CCCEEEECCEEEECCCCEEEECCCEEECHHHHCCCCEEECCCCCEEEEEECCCCCCCCCC
KWTGKMESSEAGAPATVVGAFLADKFSLTDSLHLDIGLRYDSFSYSPDGQDRNYFGSQLS
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCC
PKAMITYEFTENQSASLALYQNYRTPTIPELYWNSQSSSRDNTVNVPYLVGKDIKPETAR
CCEEEEEEECCCCCCEEEEEECCCCCCCCCEEECCCCCCCCCEEECCEEECCCCCCHHHC
GIDAAYKYSFGGKGFLKLSGFYYNIQDYIVHKPVYVNRPQSYQAWAAYNIDAEIYGATLS
CCCCEEEECCCCCEEEEEEEEEEEEEHEEEECCEEECCCCCCCEEEEEECCCEEEEEEEC
GSYDVLDNVTVKAAVTRQDSKKSNDPADPGGVMEKLEYIPDWKGTLGTTWKINEQFTLDT
CCCHHHCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCEEHHH
VLTYVGERSYYVNTAQLRKGTLHDYATLGASLSYKVDEHLTLEAYADNITNTQYEEAWGY
HHHHHCCCCEEEEHHHHCCCCCHHHHHHCCEEEEECCCCEEEEEECCCCCCCCHHHHCCC
PALGFNAGVSIKWEL
CCCCCCCCEEEEEEC
>Mature Secondary Structure
MKLILCLCLLAVTAFPQAGHAAGSDNLMQDVFVLDPVKVTGRMEEDRLDRTTAIVVEKNR
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEEEECCCCEECCCCHHHCCCEEEEEEECCC
SNNVADYLVRDPEISFKRKAAMGDSSDIISIRGMESKRIMLNLDGRNISSTGMSGGNYID
CCCCHHHEEECCCCCCHHHHCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEE
FGTIPLDNIERIEVIKGGSSVEYGNNALGGVINAYTRRPTEQPYFSVYATMGGWKDFYDF
ECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHH
HNVRGSYAQKFNAIGLSVGLSHQHADPFFRNNDYDSFHFNPKLYVDLPWRAELTLGYNYS
HHCCCHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCEEECCEEEEECCCEEEEEEECCHH
QTRRGLIRTNRNDGNPTSDSNPDLPGFDSPIDSNYPTASGESFAGGSPTPSMTVIGDGAH
HHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCH
WTKYRHLMDFTYRQEFLETAYFELSGFKNSENRREKNYADIDARMQIKKGPDGFDPSKTK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCEEEEEECCCCCCCCCCCC
NGDLVLDRNVIVDNTHGFKFKTGATIHGHELMTGVEYKRLTPGPITVEYVDKNYNKAGPN
CCCEEEECCEEEECCCCEEEECCCEEECHHHHCCCCEEECCCCCEEEEEECCCCCCCCCC
KWTGKMESSEAGAPATVVGAFLADKFSLTDSLHLDIGLRYDSFSYSPDGQDRNYFGSQLS
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCC
PKAMITYEFTENQSASLALYQNYRTPTIPELYWNSQSSSRDNTVNVPYLVGKDIKPETAR
CCEEEEEEECCCCCCEEEEEECCCCCCCCCEEECCCCCCCCCEEECCEEECCCCCCHHHC
GIDAAYKYSFGGKGFLKLSGFYYNIQDYIVHKPVYVNRPQSYQAWAAYNIDAEIYGATLS
CCCCEEEECCCCCEEEEEEEEEEEEEHEEEECCEEECCCCCCCEEEEEECCCEEEEEEEC
GSYDVLDNVTVKAAVTRQDSKKSNDPADPGGVMEKLEYIPDWKGTLGTTWKINEQFTLDT
CCCHHHCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCEEHHH
VLTYVGERSYYVNTAQLRKGTLHDYATLGASLSYKVDEHLTLEAYADNITNTQYEEAWGY
HHHHHCCCCEEEEHHHHCCCCCHHHHHHCCEEEEECCCCEEEEEECCCCCCCCHHHHCCC
PALGFNAGVSIKWEL
CCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA