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The map label for this gene is nrdE.1 [H]

Identifier: 21910585

GI number: 21910585

Start: 1090332

End: 1092491

Strand: Direct

Name: nrdE.1 [H]

Synonym: SpyM3_1049

Alternate gene names: 21910585

Gene position: 1090332-1092491 (Clockwise)

Preceding gene: 21910584

Following gene: 21910586

Centisome position: 57.37

GC content: 37.96

Gene sequence:

>2160_bases
ATGAGTCTCAAAGATCTTGGCGATATTTCATATTTTCGCCTAAATAATGAAATTAACCGTCCTGTTAATGGTAAAATTCC
ACTTCATAAAGACAAAGAAGCTTTAAAAGCTTTTTTCGCTGAAAATGTGCTGCCAAACACCATGTCTTTTACTTCCATTA
CGGAAAAAATTGAGTATTTAATCTCAAATGATTACATTGAATCAGCTTTTATTCAGAAATACCACCCTGAATTTATTACT
GAATTAGCTGGCATAATCAAATCAGAAAATTTTCGCTTTAAATCATTTATGGCAGCCTACAAGTTCTACCAGCAATACGC
CTTAAAAACAAATGATGGAGAGCATTATTTAGAAAACCTTGAAGACCGTGTCTTGTTTAATGCTTTGTATTTTGCAGATG
GTCAAGAAGACTTAGCAAAAGATTTAGCCGTTGAAATGATTAACCAACGTTACCAACCGGCTACTCCTTCCTTTTTAAAT
GCTGGTCGAAGCCGTCGTGGTGAATTGGTCTCTTGTTTCTTGATTCAAGTAACTGATGACATGAACTCTATCGGACGTTC
CATTAACTCTGCCTTGCAATTATCCCGTATTGGTGGAGGAGTTGGGATTACCTTGTCTAACCTCCGTGAAGCTGGCGCAC
CAATCAAAGGCTATGCTGGTGCAGCCTCAGGAGTTGTTCCTGTTATGAAATTATTTGAAGATAGTTTTTCTTATTCAAAT
CAACTTGGGCAACGTCAAGGAGCTGGTGTTGTTTACCTAAATGTTTTTCATCCTGATATCATTGCTTTCTTATCTACTAA
AAAAGAAAATGCCGATGAAAAGGTGCGTGTTAAAACCTTGTCACTAGGGATTACCGTTCCTGATAAATTCTACGAATTAG
CTCGTAAAAACGAGGACATGTATCTCTTTAGTCCTTACAATGTTGAAAAAGAATATGGCATTCCCTTTAACTATCTCGAC
ATTACCAATATGTACGATGAGTTAGTGGCGAACCCTAAAATTACTAAGACTAAAATTAAAGCTCGTGATCTTGAAACAGA
GATTTCAAAATTACAACAAGAATCTGGTTACCCTTATATCATCAATATTGATACAGCTAATAAAGCTAATCCTATCGATG
GAAAAATCATCATGAGCAACTTGTGTTCTGAAATTTTACAAGTTCAAACACCTAGCCTTATCAATGATGCGCAAGAGTTT
GTAGAAATGGGAACTGATATTTCATGTAACTTAGGTTCCACTAATATCCTGAACATGATGACCTCACCAGACTTTGGCCG
TTCTATTAAGACCATGACACGTGCCCTAACTTTTGTTACTGATTCATCAAGCATTGAAGCTGTTCCAACCATTAAACATG
GCAATAGCCAAGCTCATACTTTTGGCCTTGGAGCTATGGGACTACATTCTTACCTTGCTCAACATCATATTGAATATGGC
AGTCCAGAATCCATCGAGTTTACTGATATTTACTTTATGCTCCTGAATTATTGGACCTTGGTCGAATCCAATAACATCGC
TCGTGAGCGCCAAACTACCTTTGTTGGCTTTGAGAACTCTAAGTACGCTAATGGTAGTTACTTTGATAAATACGTTACAG
GACACTTTGTTCCAAAATCTGATTTGGTCAAAAGTCTCTTTAAAGATCACTTTATTCCGCAAGCTTCAGATTGGGAGGCT
CTTCGCGACGCCGTTCAAAAAGATGGTCTTTATCATCAAAACCGACTAGCAGTTGCTCCAAATGGCTCTATTTCTTATAT
CAATGACTGCTCTGCTTCTATTCACCCAATCACACAACGCATCGAAGAGCGTCAAGAAAAGAAAATTGGTAAAATCTACT
ATCCTGCAAATGGTTTGTCTACGGATACCATTCCTTACTATACATCTGCTTACGATATGGACATGCGCAAAGTTATTGAT
GTCTATGCCGCTGCGACCGAACATGTGGACCAAGGCTTGTCATTAACTCTATTCCTTCGTAGTGAGTTGCCTATGGAGCT
TTATGAGTGGAAAACACAAAGCAAACAAACCACTCGTGATTTATCCATCTTACGAAACTACGCTTTCAATAAAGGCATTA
AATCTATCTACTATATCCGTACCTTTACGGATGATGGGGAAGAAGTGGGCGCAAACCAATGTGAATCTTGTGTCATTTAA

Upstream 100 bases:

>100_bases
TTCACATCTGCTCCTGTTATCGAAGCAGATAACCTCGTATTTTCTGGCTTCCAACCTGCAAAGTTAAAAGAATTGATTTA
ATAAGACAAGGAAAAATAAT

Downstream 100 bases:

>100_bases
TTGGTTAAGAAGTAATGATGCTAAAGCATCTATTACTTCTACGGCAGTCGCTTTAGGCGATCTGCCTAAACCACTCACTA
ACTACGTAAGGAAAGTATTA

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase R1 subunit [H]

Number of amino acids: Translated: 719; Mature: 718

Protein sequence:

>719_residues
MSLKDLGDISYFRLNNEINRPVNGKIPLHKDKEALKAFFAENVLPNTMSFTSITEKIEYLISNDYIESAFIQKYHPEFIT
ELAGIIKSENFRFKSFMAAYKFYQQYALKTNDGEHYLENLEDRVLFNALYFADGQEDLAKDLAVEMINQRYQPATPSFLN
AGRSRRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGYAGAASGVVPVMKLFEDSFSYSN
QLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVRVKTLSLGITVPDKFYELARKNEDMYLFSPYNVEKEYGIPFNYLD
ITNMYDELVANPKITKTKIKARDLETEISKLQQESGYPYIINIDTANKANPIDGKIIMSNLCSEILQVQTPSLINDAQEF
VEMGTDISCNLGSTNILNMMTSPDFGRSIKTMTRALTFVTDSSSIEAVPTIKHGNSQAHTFGLGAMGLHSYLAQHHIEYG
SPESIEFTDIYFMLLNYWTLVESNNIARERQTTFVGFENSKYANGSYFDKYVTGHFVPKSDLVKSLFKDHFIPQASDWEA
LRDAVQKDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLSTDTIPYYTSAYDMDMRKVID
VYAAATEHVDQGLSLTLFLRSELPMELYEWKTQSKQTTRDLSILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI

Sequences:

>Translated_719_residues
MSLKDLGDISYFRLNNEINRPVNGKIPLHKDKEALKAFFAENVLPNTMSFTSITEKIEYLISNDYIESAFIQKYHPEFIT
ELAGIIKSENFRFKSFMAAYKFYQQYALKTNDGEHYLENLEDRVLFNALYFADGQEDLAKDLAVEMINQRYQPATPSFLN
AGRSRRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGYAGAASGVVPVMKLFEDSFSYSN
QLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVRVKTLSLGITVPDKFYELARKNEDMYLFSPYNVEKEYGIPFNYLD
ITNMYDELVANPKITKTKIKARDLETEISKLQQESGYPYIINIDTANKANPIDGKIIMSNLCSEILQVQTPSLINDAQEF
VEMGTDISCNLGSTNILNMMTSPDFGRSIKTMTRALTFVTDSSSIEAVPTIKHGNSQAHTFGLGAMGLHSYLAQHHIEYG
SPESIEFTDIYFMLLNYWTLVESNNIARERQTTFVGFENSKYANGSYFDKYVTGHFVPKSDLVKSLFKDHFIPQASDWEA
LRDAVQKDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLSTDTIPYYTSAYDMDMRKVID
VYAAATEHVDQGLSLTLFLRSELPMELYEWKTQSKQTTRDLSILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI
>Mature_718_residues
SLKDLGDISYFRLNNEINRPVNGKIPLHKDKEALKAFFAENVLPNTMSFTSITEKIEYLISNDYIESAFIQKYHPEFITE
LAGIIKSENFRFKSFMAAYKFYQQYALKTNDGEHYLENLEDRVLFNALYFADGQEDLAKDLAVEMINQRYQPATPSFLNA
GRSRRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGYAGAASGVVPVMKLFEDSFSYSNQ
LGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVRVKTLSLGITVPDKFYELARKNEDMYLFSPYNVEKEYGIPFNYLDI
TNMYDELVANPKITKTKIKARDLETEISKLQQESGYPYIINIDTANKANPIDGKIIMSNLCSEILQVQTPSLINDAQEFV
EMGTDISCNLGSTNILNMMTSPDFGRSIKTMTRALTFVTDSSSIEAVPTIKHGNSQAHTFGLGAMGLHSYLAQHHIEYGS
PESIEFTDIYFMLLNYWTLVESNNIARERQTTFVGFENSKYANGSYFDKYVTGHFVPKSDLVKSLFKDHFIPQASDWEAL
RDAVQKDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLSTDTIPYYTSAYDMDMRKVIDV
YAAATEHVDQGLSLTLFLRSELPMELYEWKTQSKQTTRDLSILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=617, Percent_Identity=24.9594813614263, Blast_Score=149, Evalue=8e-36,
Organism=Escherichia coli, GI1789030, Length=698, Percent_Identity=49.5702005730659, Blast_Score=711, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=599, Percent_Identity=25.2086811352254, Blast_Score=159, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17555242, Length=623, Percent_Identity=25.3611556982343, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6322123, Length=595, Percent_Identity=24.7058823529412, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320914, Length=602, Percent_Identity=23.421926910299, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI17352481, Length=479, Percent_Identity=26.0960334029228, Blast_Score=145, Evalue=7e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 81292; Mature: 81161

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKDLGDISYFRLNNEINRPVNGKIPLHKDKEALKAFFAENVLPNTMSFTSITEKIEYL
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ISNDYIESAFIQKYHPEFITELAGIIKSENFRFKSFMAAYKFYQQYALKTNDGEHYLENL
HHCHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
EDRVLFNALYFADGQEDLAKDLAVEMINQRYQPATPSFLNAGRSRRGELVSCFLIQVTDD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEEEEEECHH
MNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGYAGAASGVVPVMKLFEDSFSYSN
HHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVRVKTLSLGITVPDKFYELARKNEDM
HHHHCCCCCEEEEEECCHHHHHHHHCCCCCCCCEEEEEEEEEEEECCHHHHHHHHCCCCE
YLFSPYNVEKEYGIPFNYLDITNMYDELVANPKITKTKIKARDLETEISKLQQESGYPYI
EEECCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
INIDTANKANPIDGKIIMSNLCSEILQVQTPSLINDAQEFVEMGTDISCNLGSTNILNMM
EEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHCC
TSPDFGRSIKTMTRALTFVTDSSSIEAVPTIKHGNSQAHTFGLGAMGLHSYLAQHHIEYG
CCCCCCHHHHHHHHHHHEEECCCCCCCCEEEECCCCCCEEEECHHHHHHHHHHHHHCCCC
SPESIEFTDIYFMLLNYWTLVESNNIARERQTTFVGFENSKYANGSYFDKYVTGHFVPKS
CCCCEEHHHHHHHHHHHHHHCCCCCCCHHHCCEEEECCCCCCCCCCHHHHHHCCCCCCHH
DLVKSLFKDHFIPQASDWEALRDAVQKDGLYHQNRLAVAPNGSISYINDCSASIHPITQR
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEECCEEEECCCCCEEEECCCCCCHHHHHHH
IEERQEKKIGKIYYPANGLSTDTIPYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFLR
HHHHHHHCCCEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEE
SELPMELYEWKTQSKQTTRDLSILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHCCHHHHHCCC
>Mature Secondary Structure 
SLKDLGDISYFRLNNEINRPVNGKIPLHKDKEALKAFFAENVLPNTMSFTSITEKIEYL
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ISNDYIESAFIQKYHPEFITELAGIIKSENFRFKSFMAAYKFYQQYALKTNDGEHYLENL
HHCHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
EDRVLFNALYFADGQEDLAKDLAVEMINQRYQPATPSFLNAGRSRRGELVSCFLIQVTDD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEEEEEECHH
MNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGYAGAASGVVPVMKLFEDSFSYSN
HHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVRVKTLSLGITVPDKFYELARKNEDM
HHHHCCCCCEEEEEECCHHHHHHHHCCCCCCCCEEEEEEEEEEEECCHHHHHHHHCCCCE
YLFSPYNVEKEYGIPFNYLDITNMYDELVANPKITKTKIKARDLETEISKLQQESGYPYI
EEECCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
INIDTANKANPIDGKIIMSNLCSEILQVQTPSLINDAQEFVEMGTDISCNLGSTNILNMM
EEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHCC
TSPDFGRSIKTMTRALTFVTDSSSIEAVPTIKHGNSQAHTFGLGAMGLHSYLAQHHIEYG
CCCCCCHHHHHHHHHHHEEECCCCCCCCEEEECCCCCCEEEECHHHHHHHHHHHHHCCCC
SPESIEFTDIYFMLLNYWTLVESNNIARERQTTFVGFENSKYANGSYFDKYVTGHFVPKS
CCCCEEHHHHHHHHHHHHHHCCCCCCCHHHCCEEEECCCCCCCCCCHHHHHHCCCCCCHH
DLVKSLFKDHFIPQASDWEALRDAVQKDGLYHQNRLAVAPNGSISYINDCSASIHPITQR
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEECCEEEECCCCCEEEECCCCCCHHHHHHH
IEERQEKKIGKIYYPANGLSTDTIPYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFLR
HHHHHHHCCCEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEE
SELPMELYEWKTQSKQTTRDLSILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]