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The map label for this gene is insK [H]

Identifier: 21910548

GI number: 21910548

Start: 1051623

End: 1052429

Strand: Direct

Name: insK [H]

Synonym: SpyM3_1012

Alternate gene names: 21910548

Gene position: 1051623-1052429 (Clockwise)

Preceding gene: 21910547

Following gene: 21910550

Centisome position: 55.33

GC content: 39.53

Gene sequence:

>807_bases
ATGCTACTTGAAATCCTTGATTTATCACGCTCCACCTATTACTATCAAGTGAAGCGACTAGCTCAAGGAGATAAGGATAT
AGAACTAAAGCATGTGATTCGAGAGATTTATGATGAACATAAAGGTAATTACGGTTATCGTCGGATTCATATGGAGTTGT
GTAACCGAGGATTTGTGGTCAATCATAAGAAAGTTCAACGTCTCATGAAAGTCATGGGCCTAGCAGCCTGCATTCGTCGG
AAGTGCAAGTATTCTTCTTACAAAGGAGAGGTTGGTAAGAAGGCTGATAATCTGATTAAACGTCACTTCGAAGGCTCTAA
ACCTTACGAAAAATGTTACACTGATGTGACGGAGTTTGCCTTACCTGAAGGAAAACTCTACTTATCACCTGTTCTTGACG
GCTATAACAGTGAGATTATTGATTTCACTCTGTCTCGGTCTCCTAACTTGAAACAGGTTCAAACCATGCTTGAGAAGACT
TTTCCAGCAGATTCATACAGCGGAACTATTCTTCATAGTGACCAGGGGTGGCAATACCAACACCAGTCCTACCATGATTT
TTTAGAGTCTAAAGGTATTCTGGCATCCATGTCCCGCAAAGGGAATAGCCCGGATAACGGCATGATGGAATCCTTCTTTG
GCATTCTCAAATCTGAAATGTTTTATGGACTCGAGACAACTTATCAATCACTTGACAAGCTTGAAGAAGCTATTACAGAT
TACATTTTTTACTACAACAACAAACGAATCAAAGCAAAACTAAAAGGATTTAGTCCTGTCCAATACAGAACTAAATCCTT
TCAATAA

Upstream 100 bases:

>100_bases
CTGTGCTAAAAAAGCTGAGAGAATACCGCTTGAGAGACGAAGCAAAACTCAAAGAGCAACAGAAATCATTCAAGCATTAA
GGAATCAATTTCCCTTAGAG

Downstream 100 bases:

>100_bases
TTATTTGTCCAACTTTTTGGGGTCAGTACACTCCTAGTTAACGACTTTGATTGATTTTATTATGATGAATCGTGATTTCT
GAAAACAAAATTTTCTTCAT

Product: putative transposase - IS861

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MLLEILDLSRSTYYYQVKRLAQGDKDIELKHVIREIYDEHKGNYGYRRIHMELCNRGFVVNHKKVQRLMKVMGLAACIRR
KCKYSSYKGEVGKKADNLIKRHFEGSKPYEKCYTDVTEFALPEGKLYLSPVLDGYNSEIIDFTLSRSPNLKQVQTMLEKT
FPADSYSGTILHSDQGWQYQHQSYHDFLESKGILASMSRKGNSPDNGMMESFFGILKSEMFYGLETTYQSLDKLEEAITD
YIFYYNNKRIKAKLKGFSPVQYRTKSFQ

Sequences:

>Translated_268_residues
MLLEILDLSRSTYYYQVKRLAQGDKDIELKHVIREIYDEHKGNYGYRRIHMELCNRGFVVNHKKVQRLMKVMGLAACIRR
KCKYSSYKGEVGKKADNLIKRHFEGSKPYEKCYTDVTEFALPEGKLYLSPVLDGYNSEIIDFTLSRSPNLKQVQTMLEKT
FPADSYSGTILHSDQGWQYQHQSYHDFLESKGILASMSRKGNSPDNGMMESFFGILKSEMFYGLETTYQSLDKLEEAITD
YIFYYNNKRIKAKLKGFSPVQYRTKSFQ
>Mature_268_residues
MLLEILDLSRSTYYYQVKRLAQGDKDIELKHVIREIYDEHKGNYGYRRIHMELCNRGFVVNHKKVQRLMKVMGLAACIRR
KCKYSSYKGEVGKKADNLIKRHFEGSKPYEKCYTDVTEFALPEGKLYLSPVLDGYNSEIIDFTLSRSPNLKQVQTMLEKT
FPADSYSGTILHSDQGWQYQHQSYHDFLESKGILASMSRKGNSPDNGMMESFFGILKSEMFYGLETTYQSLDKLEEAITD
YIFYYNNKRIKAKLKGFSPVQYRTKSFQ

Specific function: Involved in the transposition of the insertion sequence IS150 [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=254, Percent_Identity=27.5590551181102, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI1789981, Length=267, Percent_Identity=47.1910112359551, Blast_Score=255, Evalue=2e-69,
Organism=Escherichia coli, GI1788405, Length=272, Percent_Identity=27.5735294117647, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1787263, Length=272, Percent_Identity=27.5735294117647, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1786753, Length=272, Percent_Identity=27.5735294117647, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1786570, Length=272, Percent_Identity=27.5735294117647, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1786490, Length=272, Percent_Identity=27.5735294117647, Blast_Score=97, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31340; Mature: 31340

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLEILDLSRSTYYYQVKRLAQGDKDIELKHVIREIYDEHKGNYGYRRIHMELCNRGFVV
CEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEE
NHKKVQRLMKVMGLAACIRRKCKYSSYKGEVGKKADNLIKRHFEGSKPYEKCYTDVTEFA
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHC
LPEGKLYLSPVLDGYNSEIIDFTLSRSPNLKQVQTMLEKTFPADSYSGTILHSDQGWQYQ
CCCCCEEEEHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCHH
HQSYHDFLESKGILASMSRKGNSPDNGMMESFFGILKSEMFYGLETTYQSLDKLEEAITD
HHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIFYYNNKRIKAKLKGFSPVQYRTKSFQ
HHHHCCCCEEEEEECCCCCHHHHCCCCC
>Mature Secondary Structure
MLLEILDLSRSTYYYQVKRLAQGDKDIELKHVIREIYDEHKGNYGYRRIHMELCNRGFVV
CEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEE
NHKKVQRLMKVMGLAACIRRKCKYSSYKGEVGKKADNLIKRHFEGSKPYEKCYTDVTEFA
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHC
LPEGKLYLSPVLDGYNSEIIDFTLSRSPNLKQVQTMLEKTFPADSYSGTILHSDQGWQYQ
CCCCCEEEEHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCHH
HQSYHDFLESKGILASMSRKGNSPDNGMMESFFGILKSEMFYGLETTYQSLDKLEEAITD
HHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIFYYNNKRIKAKLKGFSPVQYRTKSFQ
HHHHCCCCEEEEEECCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2841644; 2541046; 8041620; 9278503 [H]