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The map label for this gene is insI1 [C]

Identifier: 21910524

GI number: 21910524

Start: 1028695

End: 1029282

Strand: Direct

Name: insI1 [C]

Synonym: SpyM3_0988

Alternate gene names: 21910524

Gene position: 1028695-1029282 (Clockwise)

Preceding gene: 21910523

Following gene: 21910533

Centisome position: 54.13

GC content: 40.65

Gene sequence:

>588_bases
ATGGAAAACAAACCTTCTTCAACGCCTATTTCGCTGATAGTGGGCAACGTGTCTATTAAACCAATCGATTTGCCTAAGAT
GGTTAGTATCAGAAAACGTTCTAAGAAAGTCATGAAGACGAATAAGAAGACTTTAGGTAAATCTATCGAAGAACGTCCAG
AATATATTAATGATCATTCTGAATTTGGGCATTGGGAGATTGATTTGGTTCTCGGCAAGAAAACCAAAAGTGAAGCTGTT
ATGTTGACTTTGGTAGAGCGCCAGACACGCTATGCACTAGGAGTTAAGCTAGAAGACAAGCAGTCCCAAACCATTAACCG
TGCTGTCACGCATCTCATCAGTCAGTATCCTATTGCATCCATCACAGCGGATAATGGTTCTGAGTTTAGTTTACTCTCAG
ACTTAAAAGCTGTTGACGTTTACTTTGCACATCCCTATTCTTCACATGAGAGAGGAACAAACGAGAACTTCAATGGCCTC
CTCAGAGAATATGTCCCTAAAGGAGTCTCACTTAATCCACTAACCTCTGAAGAACTTGACAATGATATTACTGCCATCAA
TGAGCGCCTAAGACGACTTCTTCAATAG

Upstream 100 bases:

>100_bases
GGAAAGAAACCCGTTGAGATTGCTCGACTTCTGGGGCGTCACTGTTCTACAATCTGCCGAGAAATAAAACGTGGTTCAGT
AGATCAGGTAAAGGATAAGA

Downstream 100 bases:

>100_bases
CAATCCTCAACATTCCTGTTTGAGCTATCCCAAACAGCTTAACCTCTGGAACTCTAGTTATCGATGAACTCGTTGCACTT
GACTTGACAACTTGCGAGAG

Product: putative transposase carboxy terminal portion

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MENKPSSTPISLIVGNVSIKPIDLPKMVSIRKRSKKVMKTNKKTLGKSIEERPEYINDHSEFGHWEIDLVLGKKTKSEAV
MLTLVERQTRYALGVKLEDKQSQTINRAVTHLISQYPIASITADNGSEFSLLSDLKAVDVYFAHPYSSHERGTNENFNGL
LREYVPKGVSLNPLTSEELDNDITAINERLRRLLQ

Sequences:

>Translated_195_residues
MENKPSSTPISLIVGNVSIKPIDLPKMVSIRKRSKKVMKTNKKTLGKSIEERPEYINDHSEFGHWEIDLVLGKKTKSEAV
MLTLVERQTRYALGVKLEDKQSQTINRAVTHLISQYPIASITADNGSEFSLLSDLKAVDVYFAHPYSSHERGTNENFNGL
LREYVPKGVSLNPLTSEELDNDITAINERLRRLLQ
>Mature_195_residues
MENKPSSTPISLIVGNVSIKPIDLPKMVSIRKRSKKVMKTNKKTLGKSIEERPEYINDHSEFGHWEIDLVLGKKTKSEAV
MLTLVERQTRYALGVKLEDKQSQTINRAVTHLISQYPIASITADNGSEFSLLSDLKAVDVYFAHPYSSHERGTNENFNGL
LREYVPKGVSLNPLTSEELDNDITAINERLRRLLQ

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786450, Length=156, Percent_Identity=39.7435897435897, Blast_Score=100, Evalue=5e-23,
Organism=Escherichia coli, GI1790736, Length=156, Percent_Identity=39.1025641025641, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787671, Length=156, Percent_Identity=39.1025641025641, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 22065; Mature: 22065

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS50994 INTEGRASE ; PS01043 TRANSPOSASE_IS30

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKPSSTPISLIVGNVSIKPIDLPKMVSIRKRSKKVMKTNKKTLGKSIEERPEYINDHS
CCCCCCCCCEEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EFGHWEIDLVLGKKTKSEAVMLTLVERQTRYALGVKLEDKQSQTINRAVTHLISQYPIAS
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHCCCEE
ITADNGSEFSLLSDLKAVDVYFAHPYSSHERGTNENFNGLLREYVPKGVSLNPLTSEELD
EECCCCCCCHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHC
NDITAINERLRRLLQ
CHHHHHHHHHHHHCC
>Mature Secondary Structure
MENKPSSTPISLIVGNVSIKPIDLPKMVSIRKRSKKVMKTNKKTLGKSIEERPEYINDHS
CCCCCCCCCEEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EFGHWEIDLVLGKKTKSEAVMLTLVERQTRYALGVKLEDKQSQTINRAVTHLISQYPIAS
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHCCCEE
ITADNGSEFSLLSDLKAVDVYFAHPYSSHERGTNENFNGLLREYVPKGVSLNPLTSEELD
EECCCCCCCHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHC
NDITAINERLRRLLQ
CHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8393068 [H]