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The map label for this gene is malE [H]

Identifier: 21910519

GI number: 21910519

Start: 1024603

End: 1025850

Strand: Direct

Name: malE [H]

Synonym: SpyM3_0983

Alternate gene names: 21910519

Gene position: 1024603-1025850 (Clockwise)

Preceding gene: 21910515

Following gene: 21910520

Centisome position: 53.91

GC content: 39.02

Gene sequence:

>1248_bases
ATGAAATCATGGCAAAAAGTTATCGTCGGCGGAGCAAGTTTGACACTTGCAAGTACCTTGTTAGTGGGATGTGGATCAGG
TTCAAAAGATAAAAAGGAAGCTGGCGCTGACTCAAAGACTATTAAACTTTGGGTACCGACTGGCTCTAAAAAATCATATG
CAGATACCATTGCAAAATTTGAAAAAGATTCTGGTTACACCGTTAAAGTGGTTGAATCAGAAGATCCAAAAGCACAAGAA
AAAATCAAAAAAGATGCCTCAACTGCTGCCGATGTCTTTTCACTTCCTCATGATCAACTTGGTCAACTTGTAGAATCTGG
TACTATTCAAGAAGTTCCAGAAAAGTACAATAAAGAAATTGCTGCTACTTCTACTGATCAGGCGCTTATCGGTGCACAGT
ACAAAGGGAAAACGTATGCTTTCCCATTTGGTATTGAATCACAAGTTCTTTTCTACAATAAATCAAAATTAGCTGCTGAA
GACGTTACTTCATATGATACAATTACTACAAAAGCAACTTTTGGTGGTACCTTTAAACAAGCTAACAGTTACGCTACTGG
TCCATTATTCATGTCAGTTGGTAACACTTTATTTGGGGAAAACGGTGAAGATGTAAAAGGTACTAACTGGGGCAATGAAA
AAGGCGCTGCTGTTCTTAAATGGATCGCAGACCAAGCTTCAAATAAAGGTTTTGTTAGCTTAGATGCTAACAACGTTATC
TCTAAATTTGGTGACGGTTCAGTAGCTTCCTTTGAATCAGGACCGTGGGACTATGAAGCTGCTCAAAAAGCTATCGGTAA
AGAAAACCTTGGTGTTGCTATTTATCCAAAAGTAACTATTGGAGGCGAAACGGTTCAACAAAAAGCATTCTTGGGTGTAA
AACTTTACGCAGTCAACCAAGCACCAGCTAAAGGTGATACAAAACGTATCGCAGCTAGCTACAAATTGGCATCCTACTTG
ACTAATGCTGAAAGCCAAGAAAACCAATTCAAGACTCGTAACATTGTCCCAGCCAACAAAGAAGTTCAATCTTCAGAAGC
TGTTCAATCAAACGAACTTGCTAAAACCGTTATCACTATGGGTTCTTCTTCAGACTACACTGTCGTAATGCCAAAACTTA
GCCAAATGGGCACATTCTGGACTGAAAGTGCTGCTATTCTTAGTGATGCCTTCAACGGTAAAATCAAAGAAAGCGACTAC
CTTACTAAGTTGCAACAATTCGACAAAGATATCGCTGCAACAAAATAA

Upstream 100 bases:

>100_bases
TTTTTAAAAAAACGACCTTTTTTTTAAAAAAGGCTTGACTTTTTCTGCAAGCGGTTGCATAATATTAAGTGTAAGAAATA
TATCTTAGGAGGAAATAGCT

Downstream 100 bases:

>100_bases
TATGATCTCATGTCATCACTGCTATACTAAAACAAGCACAAGGTAAGTACTTCTAAAAAGTTCTTTCCACTTACCTTATG
AAGAGTGGAACTAGGCTCCA

Product: putative maltose/maltodextrin-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MKSWQKVIVGGASLTLASTLLVGCGSGSKDKKEAGADSKTIKLWVPTGSKKSYADTIAKFEKDSGYTVKVVESEDPKAQE
KIKKDASTAADVFSLPHDQLGQLVESGTIQEVPEKYNKEIAATSTDQALIGAQYKGKTYAFPFGIESQVLFYNKSKLAAE
DVTSYDTITTKATFGGTFKQANSYATGPLFMSVGNTLFGENGEDVKGTNWGNEKGAAVLKWIADQASNKGFVSLDANNVI
SKFGDGSVASFESGPWDYEAAQKAIGKENLGVAIYPKVTIGGETVQQKAFLGVKLYAVNQAPAKGDTKRIAASYKLASYL
TNAESQENQFKTRNIVPANKEVQSSEAVQSNELAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFNGKIKESDY
LTKLQQFDKDIAATK

Sequences:

>Translated_415_residues
MKSWQKVIVGGASLTLASTLLVGCGSGSKDKKEAGADSKTIKLWVPTGSKKSYADTIAKFEKDSGYTVKVVESEDPKAQE
KIKKDASTAADVFSLPHDQLGQLVESGTIQEVPEKYNKEIAATSTDQALIGAQYKGKTYAFPFGIESQVLFYNKSKLAAE
DVTSYDTITTKATFGGTFKQANSYATGPLFMSVGNTLFGENGEDVKGTNWGNEKGAAVLKWIADQASNKGFVSLDANNVI
SKFGDGSVASFESGPWDYEAAQKAIGKENLGVAIYPKVTIGGETVQQKAFLGVKLYAVNQAPAKGDTKRIAASYKLASYL
TNAESQENQFKTRNIVPANKEVQSSEAVQSNELAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFNGKIKESDY
LTKLQQFDKDIAATK
>Mature_415_residues
MKSWQKVIVGGASLTLASTLLVGCGSGSKDKKEAGADSKTIKLWVPTGSKKSYADTIAKFEKDSGYTVKVVESEDPKAQE
KIKKDASTAADVFSLPHDQLGQLVESGTIQEVPEKYNKEIAATSTDQALIGAQYKGKTYAFPFGIESQVLFYNKSKLAAE
DVTSYDTITTKATFGGTFKQANSYATGPLFMSVGNTLFGENGEDVKGTNWGNEKGAAVLKWIADQASNKGFVSLDANNVI
SKFGDGSVASFESGPWDYEAAQKAIGKENLGVAIYPKVTIGGETVQQKAFLGVKLYAVNQAPAKGDTKRIAASYKLASYL
TNAESQENQFKTRNIVPANKEVQSSEAVQSNELAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFNGKIKESDY
LTKLQQFDKDIAATK

Specific function: Binding protein for maltose and maltodextrin; involved in its cellular uptake [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=349, Percent_Identity=27.5071633237822, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059
- InterPro:   IPR006061 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 44540; Mature: 44540

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSWQKVIVGGASLTLASTLLVGCGSGSKDKKEAGADSKTIKLWVPTGSKKSYADTIAKF
CCCCCEEEECCCHHHHHHHHHEECCCCCCCHHHCCCCCCEEEEEECCCCCHHHHHHHHHH
EKDSGYTVKVVESEDPKAQEKIKKDASTAADVFSLPHDQLGQLVESGTIQEVPEKYNKEI
HCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCE
AATSTDQALIGAQYKGKTYAFPFGIESQVLFYNKSKLAAEDVTSYDTITTKATFGGTFKQ
ECCCCCCCEEECEECCCEEEECCCCCCEEEEEECCHHHHHHCCCCCCEEEEEECCCCHHH
ANSYATGPLFMSVGNTLFGENGEDVKGTNWGNEKGAAVLKWIADQASNKGFVSLDANNVI
CCCCCCCCCCEECCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECHHHHH
SKFGDGSVASFESGPWDYEAAQKAIGKENLGVAIYPKVTIGGETVQQKAFLGVKLYAVNQ
HHCCCCCEECCCCCCCCHHHHHHHCCCCCCCEEEEEEEEECCHHHHHHHHEEEEEEEECC
APAKGDTKRIAASYKLASYLTNAESQENQFKTRNIVPANKEVQSSEAVQSNELAKTVITM
CCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEE
GSSSDYTVVMPKLSQMGTFWTESAAILSDAFNGKIKESDYLTKLQQFDKDIAATK
CCCCCEEEECCCHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSWQKVIVGGASLTLASTLLVGCGSGSKDKKEAGADSKTIKLWVPTGSKKSYADTIAKF
CCCCCEEEECCCHHHHHHHHHEECCCCCCCHHHCCCCCCEEEEEECCCCCHHHHHHHHHH
EKDSGYTVKVVESEDPKAQEKIKKDASTAADVFSLPHDQLGQLVESGTIQEVPEKYNKEI
HCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCE
AATSTDQALIGAQYKGKTYAFPFGIESQVLFYNKSKLAAEDVTSYDTITTKATFGGTFKQ
ECCCCCCCEEECEECCCEEEECCCCCCEEEEEECCHHHHHHCCCCCCEEEEEECCCCHHH
ANSYATGPLFMSVGNTLFGENGEDVKGTNWGNEKGAAVLKWIADQASNKGFVSLDANNVI
CCCCCCCCCCEECCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECHHHHH
SKFGDGSVASFESGPWDYEAAQKAIGKENLGVAIYPKVTIGGETVQQKAFLGVKLYAVNQ
HHCCCCCEECCCCCCCCHHHHHHHCCCCCCCEEEEEEEEECCHHHHHHHHEEEEEEEECC
APAKGDTKRIAASYKLASYLTNAESQENQFKTRNIVPANKEVQSSEAVQSNELAKTVITM
CCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEE
GSSSDYTVVMPKLSQMGTFWTESAAILSDAFNGKIKESDYLTKLQQFDKDIAATK
CCCCCEEEECCCHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11463916; 2684766 [H]