Click here to switch to the map view.

The map label for this gene is potD [H]

Identifier: 21910303

GI number: 21910303

Start: 819876

End: 820949

Strand: Direct

Name: potD [H]

Synonym: SpyM3_0767

Alternate gene names: 21910303

Gene position: 819876-820949 (Clockwise)

Preceding gene: 21910302

Following gene: 21910306

Centisome position: 43.14

GC content: 37.24

Gene sequence:

>1074_bases
ATGCGTAAACTTTATTCCTTTCTAGCAGGAGTTTTGGGTGTTATTGTTATTTTAACAAGTCTTTCTTTCATCTTGCAGAA
AAAATCAGGTTCTGGTAGTCAATCGGATAAATTAGTTATTTATAACTGGGGAGATTACATTGATCCAGCTTTGCTCAAAA
AATTCACCAAAGAAACGGGCATTGAAGTGCAGTATGAAACTTTCGATTCCAATGAAGCCATGTACACTAAAATCAAGCAG
GGCGGAACCACTTATGACATTGCTGTTCCTAGTGATTACACCATTGATAAAATGATCAAAGAAAACCTACTCAATAAGCT
TGATAAGTCAAAATTAGTTGGCATGGATAATATCGGGAAAGAATTTTTAGGGAAAAGCTTTGACCCACAAAACGACTATT
CTTTGCCTTATTTCTGGGGAACCGTTGGGATTGTTTATAATGATCAATTAGTTGATAAGGCGCCTATGCACTGGGAAGAT
CTGTGGCGTCCAGAATATAAAAATAGTATTATGCTGATTGATGGAGCGCGTGAAATGCTAGGGGTTGGTTTAACAACTTT
TGGTTATAGTGTGAATTCTAAAAATCTAGAGCAGTTGCAGGCAGCCGAGAGAAAGCTGCAGCAGTTGACGCCGAATGTTA
AAGCCATTGTAGCAGATGAGATGAAAGGCTACATGATTCAAGGTGACGCTGCTATTGGAATTACCTTTTCTGGTGAAGCC
AGTGAGATGTTAGATAGTAACGAACACCTTCACTACATCGTGCCTTCAGAAGGGTCTAACCTTTGGTTTGATAATTTGGT
ACTACCAAAAACCATGAAACACGAAAAAGAAGCGTATGCTTTTTTGAACTTTATCAATCGTCCTGAAAATGCTGCGCAAA
ATGCTGCATATATTGGTTATGCGACACCAAATAAAAAAGCCAAGGCCTTACTTCCAGATGAGATAAAAAATGATCCTGCT
TTTTATCCAACAGATGACATTATCAAAAAATTGGAAGTTTATGACAATTTAGGGTCAAGATGGTTGGGGATTTATAATGA
TTTATACCTCCAATTTAAAATGTATCGCAAATAA

Upstream 100 bases:

>100_bases
ATTTCCTTGGATATCAATGCTTTGTCAACCATCGTTTTCTTTTTCTCCATCCTCTTAGTGATCGGTTATTATTATATGTC
ACAGGACAAGGAGGAAAAAC

Downstream 100 bases:

>100_bases
GCACTCAAAAAGGCTGGTATTTCTCCAGCCTTTTTAATGTATCGACAGATGACGTCACCAGTACACGCGATAGGGACGGC
CAACCTTGGTAAAAATTTGT

Product: putative spermidine/putrescine ABC transporter (periplasmic transport protein)

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MRKLYSFLAGVLGVIVILTSLSFILQKKSGSGSQSDKLVIYNWGDYIDPALLKKFTKETGIEVQYETFDSNEAMYTKIKQ
GGTTYDIAVPSDYTIDKMIKENLLNKLDKSKLVGMDNIGKEFLGKSFDPQNDYSLPYFWGTVGIVYNDQLVDKAPMHWED
LWRPEYKNSIMLIDGAREMLGVGLTTFGYSVNSKNLEQLQAAERKLQQLTPNVKAIVADEMKGYMIQGDAAIGITFSGEA
SEMLDSNEHLHYIVPSEGSNLWFDNLVLPKTMKHEKEAYAFLNFINRPENAAQNAAYIGYATPNKKAKALLPDEIKNDPA
FYPTDDIIKKLEVYDNLGSRWLGIYNDLYLQFKMYRK

Sequences:

>Translated_357_residues
MRKLYSFLAGVLGVIVILTSLSFILQKKSGSGSQSDKLVIYNWGDYIDPALLKKFTKETGIEVQYETFDSNEAMYTKIKQ
GGTTYDIAVPSDYTIDKMIKENLLNKLDKSKLVGMDNIGKEFLGKSFDPQNDYSLPYFWGTVGIVYNDQLVDKAPMHWED
LWRPEYKNSIMLIDGAREMLGVGLTTFGYSVNSKNLEQLQAAERKLQQLTPNVKAIVADEMKGYMIQGDAAIGITFSGEA
SEMLDSNEHLHYIVPSEGSNLWFDNLVLPKTMKHEKEAYAFLNFINRPENAAQNAAYIGYATPNKKAKALLPDEIKNDPA
FYPTDDIIKKLEVYDNLGSRWLGIYNDLYLQFKMYRK
>Mature_357_residues
MRKLYSFLAGVLGVIVILTSLSFILQKKSGSGSQSDKLVIYNWGDYIDPALLKKFTKETGIEVQYETFDSNEAMYTKIKQ
GGTTYDIAVPSDYTIDKMIKENLLNKLDKSKLVGMDNIGKEFLGKSFDPQNDYSLPYFWGTVGIVYNDQLVDKAPMHWED
LWRPEYKNSIMLIDGAREMLGVGLTTFGYSVNSKNLEQLQAAERKLQQLTPNVKAIVADEMKGYMIQGDAAIGITFSGEA
SEMLDSNEHLHYIVPSEGSNLWFDNLVLPKTMKHEKEAYAFLNFINRPENAAQNAAYIGYATPNKKAKALLPDEIKNDPA
FYPTDDIIKKLEVYDNLGSRWLGIYNDLYLQFKMYRK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=330, Percent_Identity=40, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1787078, Length=320, Percent_Identity=30.9375, Blast_Score=166, Evalue=3e-42,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40565; Mature: 40565

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLYSFLAGVLGVIVILTSLSFILQKKSGSGSQSDKLVIYNWGDYIDPALLKKFTKETG
CHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCC
IEVQYETFDSNEAMYTKIKQGGTTYDIAVPSDYTIDKMIKENLLNKLDKSKLVGMDNIGK
CEEEEEECCCCCCEEHEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCH
EFLGKSFDPQNDYSLPYFWGTVGIVYNDQLVDKAPMHWEDLWRPEYKNSIMLIDGAREML
HHCCCCCCCCCCCCCCEEECEEEEEECCCHHCCCCCCHHHCCCCCCCCCEEEEECCHHHH
GVGLTTFGYSVNSKNLEQLQAAERKLQQLTPNVKAIVADEMKGYMIQGDAAIGITFSGEA
HCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCEEEEEEECCCH
SEMLDSNEHLHYIVPSEGSNLWFDNLVLPKTMKHEKEAYAFLNFINRPENAAQNAAYIGY
HHHHCCCCCEEEEECCCCCCEEECCEECCHHHHHHHHHHHHHHHHCCCCCHHCCCEEEEE
ATPNKKAKALLPDEIKNDPAFYPTDDIIKKLEVYDNLGSRWLGIYNDLYLQFKMYRK
ECCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHEEEEEEEECC
>Mature Secondary Structure
MRKLYSFLAGVLGVIVILTSLSFILQKKSGSGSQSDKLVIYNWGDYIDPALLKKFTKETG
CHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCC
IEVQYETFDSNEAMYTKIKQGGTTYDIAVPSDYTIDKMIKENLLNKLDKSKLVGMDNIGK
CEEEEEECCCCCCEEHEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCH
EFLGKSFDPQNDYSLPYFWGTVGIVYNDQLVDKAPMHWEDLWRPEYKNSIMLIDGAREML
HHCCCCCCCCCCCCCCEEECEEEEEECCCHHCCCCCCHHHCCCCCCCCCEEEEECCHHHH
GVGLTTFGYSVNSKNLEQLQAAERKLQQLTPNVKAIVADEMKGYMIQGDAAIGITFSGEA
HCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCEEEEEEECCCH
SEMLDSNEHLHYIVPSEGSNLWFDNLVLPKTMKHEKEAYAFLNFINRPENAAQNAAYIGY
HHHHCCCCCEEEEECCCCCCEEECCEECCHHHHHHHHHHHHHHHHCCCCCHHCCCEEEEE
ATPNKKAKALLPDEIKNDPAFYPTDDIIKKLEVYDNLGSRWLGIYNDLYLQFKMYRK
ECCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]