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The map label for this gene is potB [H]

Identifier: 21910301

GI number: 21910301

Start: 818316

End: 819110

Strand: Direct

Name: potB [H]

Synonym: SpyM3_0765

Alternate gene names: 21910301

Gene position: 818316-819110 (Clockwise)

Preceding gene: 21910300

Following gene: 21910302

Centisome position: 43.06

GC content: 39.75

Gene sequence:

>795_bases
ATGAAGAAAACCTCTAGTCTTTTTTCGATTCCTTACTTCTTATGGATTCTCTTCTTTGTTGTGGCACCAGTCACTCTCTT
GTTTTACAAGTCCTTTTTTGACATAGAAGGGCGCGTGACCTTAGCCAATTATGAAACCTTTTTTAGCTCTTGGACCTATT
TGAGAATGAGTGTGAATTCTATTTTATACGCTGGTATTATCACACTCGTCACGCTCTTGATTTCATATCCTACGGCTCTC
TTTTTAACGCGCCTAAAGCACAAGCAGTTGTGGCTTATGCTCATTATTTTGCCAACTTGGGTAAACTTATTGCTAAAAGC
CTATGCCTTTATGGGAATCTTTGGTCAACAAGGAGGAATTAACAGCTTTTTAACCTTTATGGGGATTGGCCCGCAGCAAA
TCCTTTTCACGGATTTCTCCTTCATTTTTGTAGCCTCTTACATTGAGCTTCCTTTTATGATGTTACCGATTTTTAATGCT
TTGGATGATATTGACCATAATGTCATCAATGCCAGTCGCGATCTAGGAGCTAGTGAATTTCAGGCCTTCTCAAAAGTTAT
TTTTCCCCTTTCTTTAAATGGGGTTAGGGCAGGTGTTCAGTCTGTCTTTATCCCAAGTTTGAGTCTCTTTATGTTAACCC
GTTTGATTGGTGGAAACCGCGTGATTACACTTGGTACAGCCATTGAACAACATTTTTTGACCACCCAAAACTGGGGAATG
GGATCAACCATTGGTGTCGTCTTGATTTTGACCATGGTTGCTATTATGTGGCTCACAAAGGAGAAAAGTAAATGA

Upstream 100 bases:

>100_bases
CTTAATGAGACTGAAGAGGAATTTGATGCCCGTATTGAAGAATATGTGGAAATGGATGAGCCTGAAGATGGATTGATTAA
TGCCATTGAGGAGGAGCGTA

Downstream 100 bases:

>100_bases
AAAAATTTGCCAATCTTTATTTAGCGAGTGTCTTTGTTTTACTCTACATTCCTATTTTTTATTTGATTTTCTATTCTTTC
AACAAAGGTGGGGATATGAA

Product: putative spermidine/putrescine ABC transporter (permease protein)

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKKTSSLFSIPYFLWILFFVVAPVTLLFYKSFFDIEGRVTLANYETFFSSWTYLRMSVNSILYAGIITLVTLLISYPTAL
FLTRLKHKQLWLMLIILPTWVNLLLKAYAFMGIFGQQGGINSFLTFMGIGPQQILFTDFSFIFVASYIELPFMMLPIFNA
LDDIDHNVINASRDLGASEFQAFSKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGGNRVITLGTAIEQHFLTTQNWGM
GSTIGVVLILTMVAIMWLTKEKSK

Sequences:

>Translated_264_residues
MKKTSSLFSIPYFLWILFFVVAPVTLLFYKSFFDIEGRVTLANYETFFSSWTYLRMSVNSILYAGIITLVTLLISYPTAL
FLTRLKHKQLWLMLIILPTWVNLLLKAYAFMGIFGQQGGINSFLTFMGIGPQQILFTDFSFIFVASYIELPFMMLPIFNA
LDDIDHNVINASRDLGASEFQAFSKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGGNRVITLGTAIEQHFLTTQNWGM
GSTIGVVLILTMVAIMWLTKEKSK
>Mature_264_residues
MKKTSSLFSIPYFLWILFFVVAPVTLLFYKSFFDIEGRVTLANYETFFSSWTYLRMSVNSILYAGIITLVTLLISYPTAL
FLTRLKHKQLWLMLIILPTWVNLLLKAYAFMGIFGQQGGINSFLTFMGIGPQQILFTDFSFIFVASYIELPFMMLPIFNA
LDDIDHNVINASRDLGASEFQAFSKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGGNRVITLGTAIEQHFLTTQNWGM
GSTIGVVLILTMVAIMWLTKEKSK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=248, Percent_Identity=33.0645161290323, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1787080, Length=249, Percent_Identity=33.7349397590361, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1787713, Length=219, Percent_Identity=29.2237442922374, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29874; Mature: 29874

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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CHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]