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The map label for this gene is dacA [H]

Identifier: 21910291

GI number: 21910291

Start: 809869

End: 811116

Strand: Direct

Name: dacA [H]

Synonym: SpyM3_0755

Alternate gene names: 21910291

Gene position: 809869-811116 (Clockwise)

Preceding gene: 21910290

Following gene: 21910293

Centisome position: 42.61

GC content: 40.38

Gene sequence:

>1248_bases
ATGAAAGATATAATGATAAAAAGGTGGGCTATCCTTTTTTTTGTGGTATTAACTTGTTCAGGGCTTAGTAAAATAGTCCT
CGCAGCTGATTTTCAAGCGGGAGCAGAACATGCGATTGTCGTAGAGGCAGACTCTGGGAGAGTTCTGTATGAGAAAGACG
CTAAGACGCCAGATGCTATTGCCTCTTTGACCAAATTGGTGACGGCTTATCTGGTTTTAGATAAGGTTAAATCAGGCCAG
CTGCAATTATCAGATCAAGTTAATCTCTCAGATTATGCCTTCGAGTTAACAAAAGATAGGTCTTTAAGTAATGTACCTTT
TGACAAAAAAACTTATTCAGTTCAAGACCTGTTGACAGCTACTTTGGTAGCAAGCTCAAATAGCGCTGCTATTGCGTTGG
CCGAAAAAGTGGCGGGCTCAGAGCCTCATTTTGTAAATCAAATGAGAGAACAGCTATCTCATTGGGGGATTACTTCTGGT
AAAATCCTCAACGCCTCAGGTCTGCCAAATGAGGTGTTAAAGGACCATCGTTATCCTGGTTCTGCGTTGGAAGAAGAAAA
TACCTTGAGTGCTCAAGATGTTGCAGTTGTGACGATGCATTTGTTAGAAGATTTTCCTGAGATTTTAGAGATCACCAAAC
AAACAGAAGTTAATTTTGCTGGCAATTCTATTAAAAGTTTTAATCAACTCTTACCAGGTATGGCTAAGGGTAGAGCAGGA
GTGGATGGTTTAAAGACTGGGACAACAGACTTGGCAGGTCATTGTCTTGTGGTAACTTCTATTGAAAATAGTATGCGAGT
GATTACCGTTATTCTAAATGCCGATGGATCTGATAAAAACCAAAACACGCGGTTTGAGCAAGCAAATCGTTTGTTGGACT
ATGTGGCTAGAACTTATTGTCGTCGTAAGATTCTTAAAAAAGGAAGTCTTGTCTCGGAGCGATCGCTCCCTATTCAAGAT
GGCCAAGTAAAAGAACTACCGGTATCTGTTGCCGAAGATGTAACCATCATTTTGCAACAGGGTGAACAAGTCCCCAAACC
TAAACAATTTATGATATCAGAGACTTCTTTACTAGCACCGATTACTAAAGGTGAAGTGGTTGCTTATTTGACGTCTCCAC
GAATAACAGATCAGTCAGTGAGGTATTTAAAGGAACCTAAGCGTATTCCTCTTAAAGCTAGCCAATCTCTTAAAAAAGCC
TCTGACTTACAGCTGTGGTGGCGAGATTTTCTTGAAAAAAGACGTTAA

Upstream 100 bases:

>100_bases
AGTTAATAATGTTAAGCTTTAAGTGTAATTGAGATTATTTTGATAAGTGGTATTTCACTTATAGTCAATTTTTCTGAGAA
TTTTTGCTATAATAGTAACT

Downstream 100 bases:

>100_bases
AGATAAGAATAAAATAATTTTAAAACAATCAAAAAAGACAGTTATGAAAGAATTTTTTCATAACTGTCTTTTTAGTTTAC
AGTGAGTATGATTTGGAATG

Product: putative D,D-carboxypeptidase, penicillin-binding protein

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MKDIMIKRWAILFFVVLTCSGLSKIVLAADFQAGAEHAIVVEADSGRVLYEKDAKTPDAIASLTKLVTAYLVLDKVKSGQ
LQLSDQVNLSDYAFELTKDRSLSNVPFDKKTYSVQDLLTATLVASSNSAAIALAEKVAGSEPHFVNQMREQLSHWGITSG
KILNASGLPNEVLKDHRYPGSALEEENTLSAQDVAVVTMHLLEDFPEILEITKQTEVNFAGNSIKSFNQLLPGMAKGRAG
VDGLKTGTTDLAGHCLVVTSIENSMRVITVILNADGSDKNQNTRFEQANRLLDYVARTYCRRKILKKGSLVSERSLPIQD
GQVKELPVSVAEDVTIILQQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKASQSLKKA
SDLQLWWRDFLEKRR

Sequences:

>Translated_415_residues
MKDIMIKRWAILFFVVLTCSGLSKIVLAADFQAGAEHAIVVEADSGRVLYEKDAKTPDAIASLTKLVTAYLVLDKVKSGQ
LQLSDQVNLSDYAFELTKDRSLSNVPFDKKTYSVQDLLTATLVASSNSAAIALAEKVAGSEPHFVNQMREQLSHWGITSG
KILNASGLPNEVLKDHRYPGSALEEENTLSAQDVAVVTMHLLEDFPEILEITKQTEVNFAGNSIKSFNQLLPGMAKGRAG
VDGLKTGTTDLAGHCLVVTSIENSMRVITVILNADGSDKNQNTRFEQANRLLDYVARTYCRRKILKKGSLVSERSLPIQD
GQVKELPVSVAEDVTIILQQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKASQSLKKA
SDLQLWWRDFLEKRR
>Mature_415_residues
MKDIMIKRWAILFFVVLTCSGLSKIVLAADFQAGAEHAIVVEADSGRVLYEKDAKTPDAIASLTKLVTAYLVLDKVKSGQ
LQLSDQVNLSDYAFELTKDRSLSNVPFDKKTYSVQDLLTATLVASSNSAAIALAEKVAGSEPHFVNQMREQLSHWGITSG
KILNASGLPNEVLKDHRYPGSALEEENTLSAQDVAVVTMHLLEDFPEILEITKQTEVNFAGNSIKSFNQLLPGMAKGRAG
VDGLKTGTTDLAGHCLVVTSIENSMRVITVILNADGSDKNQNTRFEQANRLLDYVARTYCRRKILKKGSLVSERSLPIQD
GQVKELPVSVAEDVTIILQQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKASQSLKKA
SDLQLWWRDFLEKRR

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=366, Percent_Identity=30.6010928961749, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1786851, Length=341, Percent_Identity=27.5659824046921, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87082022, Length=374, Percent_Identity=26.4705882352941, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 45845; Mature: 45845

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDIMIKRWAILFFVVLTCSGLSKIVLAADFQAGAEHAIVVEADSGRVLYEKDAKTPDAI
CCCHHHHHHHHHHHHHHHHCCHHHEEEEECCCCCCCEEEEEECCCCCEEEECCCCCHHHH
ASLTKLVTAYLVLDKVKSGQLQLSDQVNLSDYAFELTKDRSLSNVPFDKKTYSVQDLLTA
HHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHEEECCCCCCCCCCCCCCHHHHHHHHHH
TLVASSNSAAIALAEKVAGSEPHFVNQMREQLSHWGITSGKILNASGLPNEVLKDHRYPG
HHHCCCCCCEEEEHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHCCCCC
SALEEENTLSAQDVAVVTMHLLEDFPEILEITKQTEVNFAGNSIKSFNQLLPGMAKGRAG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHCCCHHCCCCC
VDGLKTGTTDLAGHCLVVTSIENSMRVITVILNADGSDKNQNTRFEQANRLLDYVARTYC
CCCCCCCCHHHCCCEEEEEECCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
RRKILKKGSLVSERSLPIQDGQVKELPVSVAEDVTIILQQGEQVPKPKQFMISETSLLAP
HHHHHHCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHEECCCCCCCCCHHHEECCHHHCCC
ITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKASQSLKKASDLQLWWRDFLEKRR
CCCCCEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKDIMIKRWAILFFVVLTCSGLSKIVLAADFQAGAEHAIVVEADSGRVLYEKDAKTPDAI
CCCHHHHHHHHHHHHHHHHCCHHHEEEEECCCCCCCEEEEEECCCCCEEEECCCCCHHHH
ASLTKLVTAYLVLDKVKSGQLQLSDQVNLSDYAFELTKDRSLSNVPFDKKTYSVQDLLTA
HHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHEEECCCCCCCCCCCCCCHHHHHHHHHH
TLVASSNSAAIALAEKVAGSEPHFVNQMREQLSHWGITSGKILNASGLPNEVLKDHRYPG
HHHCCCCCCEEEEHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHCCCCC
SALEEENTLSAQDVAVVTMHLLEDFPEILEITKQTEVNFAGNSIKSFNQLLPGMAKGRAG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHCCCHHCCCCC
VDGLKTGTTDLAGHCLVVTSIENSMRVITVILNADGSDKNQNTRFEQANRLLDYVARTYC
CCCCCCCCHHHCCCEEEEEECCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
RRKILKKGSLVSERSLPIQDGQVKELPVSVAEDVTIILQQGEQVPKPKQFMISETSLLAP
HHHHHHCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHEECCCCCCCCCHHHEECCHHHCCC
ITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKASQSLKKASDLQLWWRDFLEKRR
CCCCCEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]