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The map label for this gene is flgJ [H]

Identifier: 21910267

GI number: 21910267

Start: 785034

End: 786242

Strand: Direct

Name: flgJ [H]

Synonym: SpyM3_0731

Alternate gene names: 21910267

Gene position: 785034-786242 (Clockwise)

Preceding gene: 21910266

Following gene: 21910272

Centisome position: 41.31

GC content: 44.5

Gene sequence:

>1209_bases
ATGACCTTTTTAGATAAAATTAAACAAGGCTGTTTAGATGGCTGGGCTAAGTACAAAATCTTGCCATCCTTGACCGCAGC
ACAAGCTATCTTAGAGAGCGGGTGGGGCAAACATGCCCCACACAACGCTCTGTTTGGTATTAAGGCAGATAGCTCTTGGA
CTGGTAAATCATTTGATACCAAAACCCAAGAGGAATATCAAGCAGGTGTTGTCACGGATATTGTGGACCGATTTAGGGCG
TATGATAGTTGGACTGACAGCATTATTGATCACGGTAAATTTTTAAACGATAATCCACGGTATAAGGCAGTCGTTGGTGA
GACTGACTATAAAAAGGCTTGCCATGCTATTAAAGATGCAGGGTATGCCACAGCAAGTGGCTATGCGGAGCTACTTATCC
AAATTATCAAGGAGAATGGCTTGCAGTTTTGGGATGCCGAAGTCTTAAAAAGTAATAAGGAGGAGAAAATGATTAGTTCT
CAATGTCGAGAAGTTATCGAATTTTTTATTAATTTGGCCAATGCTGGTATGGGTGTTGACAAAGATAGTTTTGCGGGCTG
GCAATGTGCAGATGTGCCTTGTTATGCAGCAAAGCACTGGTTTGGTGTTGATCTTTGGGGAAATGCGATTGACTTACTAG
ATAGCGCTGCTGCCGTAGGTTGGGAAGTCCACCGCATGCCGACAGATGCAAATCCACTGACGGGAGCATTTTTTGTCCAA
TCAGTGCCGTATCACCAATTTGGACATACGGGAATTGTTATCGAGGATAGTGACGGTTACACCATGCGCACTGTCGAGCA
AAACATTGATGGCAATCCTGATGCTTTGTATGTCGGTGCACCAGCTCGTTTTAACACTCGTGACTTTACTGGCGTGATAG
GTTGGTTTTACCCACCATATCAAGGGGATACAGTCACGCAACCAGTCAGCACCGAGCCGCAAACTTCTGACACTATCGTA
GAGACAGCAAAAACAGGCACCTTTACCCTTGATGTTGCAGAGATCAATATCAGACGCTGGCCAAGTCTAGCCAGCGAGGT
TGTAGGTATCTACAAGCAAGGTGATACTGTCAGCTTTGATAGCGAGGGCTACGCTAATGGCTATTATTGGATTAGCTATG
TTGGAGGCTTAGGTATGCGTGACTACCTAGCTATTGGACAGACTGATAAAGACGGGAACATTATCAGTCTTTGGGGTAAA
TTAGTGTAA

Upstream 100 bases:

>100_bases
CAATCACACGATTAGCATTAGTTATAGCAATAGCAATACTGTATGTGCCATTATCTGTTGTTGCTCTGATCTTTTATCCA
TTTTTAGATAAGGAGGACAG

Downstream 100 bases:

>100_bases
CCGACATATATGCCGGTAGCAAAAAAGGAGGTAAAGCTCCAAGATAAGACAAAACCGCTCAGATTAATTTCTGGGCGGTT
TTTTGTGTGTCTAAAAAAGC

Product: putative holin - phage associated

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTFLDKIKQGCLDGWAKYKILPSLTAAQAILESGWGKHAPHNALFGIKADSSWTGKSFDTKTQEEYQAGVVTDIVDRFRA
YDSWTDSIIDHGKFLNDNPRYKAVVGETDYKKACHAIKDAGYATASGYAELLIQIIKENGLQFWDAEVLKSNKEEKMISS
QCREVIEFFINLANAGMGVDKDSFAGWQCADVPCYAAKHWFGVDLWGNAIDLLDSAAAVGWEVHRMPTDANPLTGAFFVQ
SVPYHQFGHTGIVIEDSDGYTMRTVEQNIDGNPDALYVGAPARFNTRDFTGVIGWFYPPYQGDTVTQPVSTEPQTSDTIV
ETAKTGTFTLDVAEINIRRWPSLASEVVGIYKQGDTVSFDSEGYANGYYWISYVGGLGMRDYLAIGQTDKDGNIISLWGK
LV

Sequences:

>Translated_402_residues
MTFLDKIKQGCLDGWAKYKILPSLTAAQAILESGWGKHAPHNALFGIKADSSWTGKSFDTKTQEEYQAGVVTDIVDRFRA
YDSWTDSIIDHGKFLNDNPRYKAVVGETDYKKACHAIKDAGYATASGYAELLIQIIKENGLQFWDAEVLKSNKEEKMISS
QCREVIEFFINLANAGMGVDKDSFAGWQCADVPCYAAKHWFGVDLWGNAIDLLDSAAAVGWEVHRMPTDANPLTGAFFVQ
SVPYHQFGHTGIVIEDSDGYTMRTVEQNIDGNPDALYVGAPARFNTRDFTGVIGWFYPPYQGDTVTQPVSTEPQTSDTIV
ETAKTGTFTLDVAEINIRRWPSLASEVVGIYKQGDTVSFDSEGYANGYYWISYVGGLGMRDYLAIGQTDKDGNIISLWGK
LV
>Mature_401_residues
TFLDKIKQGCLDGWAKYKILPSLTAAQAILESGWGKHAPHNALFGIKADSSWTGKSFDTKTQEEYQAGVVTDIVDRFRAY
DSWTDSIIDHGKFLNDNPRYKAVVGETDYKKACHAIKDAGYATASGYAELLIQIIKENGLQFWDAEVLKSNKEEKMISSQ
CREVIEFFINLANAGMGVDKDSFAGWQCADVPCYAAKHWFGVDLWGNAIDLLDSAAAVGWEVHRMPTDANPLTGAFFVQS
VPYHQFGHTGIVIEDSDGYTMRTVEQNIDGNPDALYVGAPARFNTRDFTGVIGWFYPPYQGDTVTQPVSTEPQTSDTIVE
TAKTGTFTLDVAEINIRRWPSLASEVVGIYKQGDTVSFDSEGYANGYYWISYVGGLGMRDYLAIGQTDKDGNIISLWGKL
V

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=122, Percent_Identity=36.8852459016393, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 44492; Mature: 44361

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50911 CHAP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFLDKIKQGCLDGWAKYKILPSLTAAQAILESGWGKHAPHNALFGIKADSSWTGKSFDT
CCHHHHHHHHHHCCHHHEEECCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCC
KTQEEYQAGVVTDIVDRFRAYDSWTDSIIDHGKFLNDNPRYKAVVGETDYKKACHAIKDA
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHC
GYATASGYAELLIQIIKENGLQFWDAEVLKSNKEEKMISSQCREVIEFFINLANAGMGVD
CCCCHHHHHHHHHHHHHHCCCEEECHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
KDSFAGWQCADVPCYAAKHWFGVDLWGNAIDLLDSAAAVGWEVHRMPTDANPLTGAFFVQ
CCCCCCCEECCCCHHHHHHEEEEEHHCCHHHHHHHHHHHCEEEEECCCCCCCCCHHHHHC
SVPYHQFGHTGIVIEDSDGYTMRTVEQNIDGNPDALYVGAPARFNTRDFTGVIGWFYPPY
CCCHHHCCCEEEEEECCCCCEEEEEHHCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCC
QGDTVTQPVSTEPQTSDTIVETAKTGTFTLDVAEINIRRWPSLASEVVGIYKQGDTVSFD
CCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEEEECHHHCHHHHHHHHHHHCCCCEEEEC
SEGYANGYYWISYVGGLGMRDYLAIGQTDKDGNIISLWGKLV
CCCCCCCEEEEEECCCCCCHHHHEECCCCCCCCEEEECCCCC
>Mature Secondary Structure 
TFLDKIKQGCLDGWAKYKILPSLTAAQAILESGWGKHAPHNALFGIKADSSWTGKSFDT
CHHHHHHHHHHCCHHHEEECCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCC
KTQEEYQAGVVTDIVDRFRAYDSWTDSIIDHGKFLNDNPRYKAVVGETDYKKACHAIKDA
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHC
GYATASGYAELLIQIIKENGLQFWDAEVLKSNKEEKMISSQCREVIEFFINLANAGMGVD
CCCCHHHHHHHHHHHHHHCCCEEECHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
KDSFAGWQCADVPCYAAKHWFGVDLWGNAIDLLDSAAAVGWEVHRMPTDANPLTGAFFVQ
CCCCCCCEECCCCHHHHHHEEEEEHHCCHHHHHHHHHHHCEEEEECCCCCCCCCHHHHHC
SVPYHQFGHTGIVIEDSDGYTMRTVEQNIDGNPDALYVGAPARFNTRDFTGVIGWFYPPY
CCCHHHCCCEEEEEECCCCCEEEEEHHCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCC
QGDTVTQPVSTEPQTSDTIVETAKTGTFTLDVAEINIRRWPSLASEVVGIYKQGDTVSFD
CCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEEEECHHHCHHHHHHHHHHHCCCCEEEEC
SEGYANGYYWISYVGGLGMRDYLAIGQTDKDGNIISLWGKLV
CCCCCCCEEEEEECCCCCCHHHHEECCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]