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The map label for this gene is mvaS.2 [H]

Identifier: 21910136

GI number: 21910136

Start: 660150

End: 661325

Strand: Reverse

Name: mvaS.2 [H]

Synonym: SpyM3_0600

Alternate gene names: 21910136

Gene position: 661325-660150 (Counterclockwise)

Preceding gene: 21910143

Following gene: 21910135

Centisome position: 34.8

GC content: 37.59

Gene sequence:

>1176_bases
ATGACAATTGGAATTGATAAGATTGGTTTTGCAACCAGTCAATATGTCTTGAAATTGGAAGATTTAGCGCTTGCACGCCA
AGTGGATCCAGCAAAATTTAGTCAAGGGCTACTCATTGAATCTTTTAGTGTGGCACCAATCACTGAAGACATTATTACTT
TAGCTGCTTCTGCAGCAGATCAAATCTTAACCGACGAAGATCGAGCTAAGATTGATATGGTTATTTTGGCAACTGAATCG
AGTACTGATCAGTCAAAGGCATCAGCTATCTATGTACATCACTTAGTTGGTATCCAGCCTTTTGCACGTTCCTTTGAAGT
AAAACAAGCCTGCTATAGCGCAACTGCTGCTCTAGACTATGCTAAACTGCATGTGGCTTCTAAGCCAGATTCTCGTGTCC
TTGTTATTGCTAGTGATATTGCTAGATACGGTGTAGGATCTCCTGGCGAATCAACTCAAGGATCTGGTAGTATTGCTCTT
TTGGTAACTGCTGACCCTCGTATTCTTGCTCTAAATGAAGATAATGTGGCTCAAACTAGGGATATTATGGACTTTTGGAG
GCCTAACTATAGTTTCACACCTTATGTTGATGGTATTTACTCTACCAAGCAATATCTCAATTGCTTAGAAACCACGTGGC
AAGCTTATCAGAAAAGAGAAAACCTTCAGTTATCTGATTTTGCTGCAGTTTGTTTCCATATTCCATTTCCCAAGTTAGCC
CTAAAAGGTCTAAATAACATTATGGATAACACAGTACCTCCTGAACACAGGGAAAAACTAATAGAAGCCTTTCAAGCTTC
TATTACTTATAGCAAACAAATTGGAAATATTTATACTGGCTCACTTTATCTAGGATTGTTATCTTTACTTGAAAATAGTA
AAGTATTACAATCTGGAGATAAAATTGGTTTTTTTAGCTATGGTTCCGGTGCCGTAAGTGAATTTTATTCCGGTCAGTTA
GTTGCTGGGTACGATAAAATGTTAATGACTAATCGACAAGCTTTACTAGATCAACGAACACGTCTTTCCGTTTCTAAATA
CGAAGACCTTTTCTACGAACAAGTCCAATTAGATGATAATGGTAATGCCAATTTTGACATTTACTTAACTGGAAAATTTG
CTCTAACAGCCATCAAGGAGCATCAAAGGATCTATCATACCAATGACAAAAACTAA

Upstream 100 bases:

>100_bases
AACTATTAAAGGATAAAACAAATAACTTTATCAGTCTAATTTTGCAGTAAAATGTGATAAAATGGGTAAGACTATAAAAA
TAACTAAAAGAAGGACAAAT

Downstream 100 bases:

>100_bases
TCTTAACTGGAGCGGTTTTTCAAAGAAAACATTCGAAGAACGCCTCCAACTTATCGAAAAATTTAAACTACTTAATGCTG
AAAACTTAAATCAACTCAAA

Product: putative 3-hydroxy-3-methylglutaryl-coenzyme A synthase

Products: (S)-3-hydroxy-3-methylglutaryl-CoA; CoA

Alternate protein names: HMG synthase [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MTIGIDKIGFATSQYVLKLEDLALARQVDPAKFSQGLLIESFSVAPITEDIITLAASAADQILTDEDRAKIDMVILATES
STDQSKASAIYVHHLVGIQPFARSFEVKQACYSATAALDYAKLHVASKPDSRVLVIASDIARYGVGSPGESTQGSGSIAL
LVTADPRILALNEDNVAQTRDIMDFWRPNYSFTPYVDGIYSTKQYLNCLETTWQAYQKRENLQLSDFAAVCFHIPFPKLA
LKGLNNIMDNTVPPEHREKLIEAFQASITYSKQIGNIYTGSLYLGLLSLLENSKVLQSGDKIGFFSYGSGAVSEFYSGQL
VAGYDKMLMTNRQALLDQRTRLSVSKYEDLFYEQVQLDDNGNANFDIYLTGKFALTAIKEHQRIYHTNDKN

Sequences:

>Translated_391_residues
MTIGIDKIGFATSQYVLKLEDLALARQVDPAKFSQGLLIESFSVAPITEDIITLAASAADQILTDEDRAKIDMVILATES
STDQSKASAIYVHHLVGIQPFARSFEVKQACYSATAALDYAKLHVASKPDSRVLVIASDIARYGVGSPGESTQGSGSIAL
LVTADPRILALNEDNVAQTRDIMDFWRPNYSFTPYVDGIYSTKQYLNCLETTWQAYQKRENLQLSDFAAVCFHIPFPKLA
LKGLNNIMDNTVPPEHREKLIEAFQASITYSKQIGNIYTGSLYLGLLSLLENSKVLQSGDKIGFFSYGSGAVSEFYSGQL
VAGYDKMLMTNRQALLDQRTRLSVSKYEDLFYEQVQLDDNGNANFDIYLTGKFALTAIKEHQRIYHTNDKN
>Mature_390_residues
TIGIDKIGFATSQYVLKLEDLALARQVDPAKFSQGLLIESFSVAPITEDIITLAASAADQILTDEDRAKIDMVILATESS
TDQSKASAIYVHHLVGIQPFARSFEVKQACYSATAALDYAKLHVASKPDSRVLVIASDIARYGVGSPGESTQGSGSIALL
VTADPRILALNEDNVAQTRDIMDFWRPNYSFTPYVDGIYSTKQYLNCLETTWQAYQKRENLQLSDFAAVCFHIPFPKLAL
KGLNNIMDNTVPPEHREKLIEAFQASITYSKQIGNIYTGSLYLGLLSLLENSKVLQSGDKIGFFSYGSGAVSEFYSGQLV
AGYDKMLMTNRQALLDQRTRLSVSKYEDLFYEQVQLDDNGNANFDIYLTGKFALTAIKEHQRIYHTNDKN

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the aldol condensation between the acetyl group attached to the acyl-carrier-protein AcpK (Ac- AcpK) and

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA synthase family [H]

Homologues:

Organism=Homo sapiens, GI148298764, Length=450, Percent_Identity=26.6666666666667, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI148298677, Length=450, Percent_Identity=26.6666666666667, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI5031751, Length=446, Percent_Identity=25.7847533632287, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17560126, Length=392, Percent_Identity=24.234693877551, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323509, Length=377, Percent_Identity=24.9336870026525, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24654139, Length=403, Percent_Identity=27.2952853598015, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24654137, Length=403, Percent_Identity=27.2952853598015, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24654133, Length=403, Percent_Identity=27.2952853598015, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24654135, Length=403, Percent_Identity=27.2952853598015, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI17933694, Length=403, Percent_Identity=27.2952853598015, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013746
- InterPro:   IPR013528
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF08540 HMG_CoA_synt_C; PF01154 HMG_CoA_synt_N [H]

EC number: 2.3.3.10

Molecular weight: Translated: 43378; Mature: 43246

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIGIDKIGFATSQYVLKLEDLALARQVDPAKFSQGLLIESFSVAPITEDIITLAASAAD
CEECCCCCCCCCHHHHEEHHHHHHHHCCCHHHHCCCCEEECCCCCCHHHHHHHHHHHHHH
QILTDEDRAKIDMVILATESSTDQSKASAIYVHHLVGIQPFARSFEVKQACYSATAALDY
HHCCCCCCCEEEEEEEEECCCCCCHHHHEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AKLHVASKPDSRVLVIASDIARYGVGSPGESTQGSGSIALLVTADPRILALNEDNVAQTR
HEEEECCCCCCCEEEEECHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCHHHHH
DIMDFWRPNYSFTPYVDGIYSTKQYLNCLETTWQAYQKRENLQLSDFAAVCFHIPFPKLA
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHH
LKGLNNIMDNTVPPEHREKLIEAFQASITYSKQIGNIYTGSLYLGLLSLLENSKVLQSGD
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCHHHHCCC
KIGFFSYGSGAVSEFYSGQLVAGYDKMLMTNRQALLDQRTRLSVSKYEDLFYEQVQLDDN
CEEEEECCCCHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
GNANFDIYLTGKFALTAIKEHQRIYHTNDKN
CCCEEEEEEECHHHHHHHHHHHHEECCCCCC
>Mature Secondary Structure 
TIGIDKIGFATSQYVLKLEDLALARQVDPAKFSQGLLIESFSVAPITEDIITLAASAAD
EECCCCCCCCCHHHHEEHHHHHHHHCCCHHHHCCCCEEECCCCCCHHHHHHHHHHHHHH
QILTDEDRAKIDMVILATESSTDQSKASAIYVHHLVGIQPFARSFEVKQACYSATAALDY
HHCCCCCCCEEEEEEEEECCCCCCHHHHEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AKLHVASKPDSRVLVIASDIARYGVGSPGESTQGSGSIALLVTADPRILALNEDNVAQTR
HEEEECCCCCCCEEEEECHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCHHHHH
DIMDFWRPNYSFTPYVDGIYSTKQYLNCLETTWQAYQKRENLQLSDFAAVCFHIPFPKLA
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHH
LKGLNNIMDNTVPPEHREKLIEAFQASITYSKQIGNIYTGSLYLGLLSLLENSKVLQSGD
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCHHHHCCC
KIGFFSYGSGAVSEFYSGQLVAGYDKMLMTNRQALLDQRTRLSVSKYEDLFYEQVQLDDN
CEEEEECCCCHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
GNANFDIYLTGKFALTAIKEHQRIYHTNDKN
CCCEEEEEEECHHHHHHHHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; H2O; acetoacetyl-CoA

Specific reaction: acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]