Click here to switch to the map view.

The map label for this gene is agaF [H]

Identifier: 21909983

GI number: 21909983

Start: 491392

End: 491829

Strand: Reverse

Name: agaF [H]

Synonym: SpyM3_0447

Alternate gene names: 21909983

Gene position: 491829-491392 (Counterclockwise)

Preceding gene: 21909993

Following gene: 21909982

Centisome position: 25.88

GC content: 42.01

Gene sequence:

>438_bases
ATGATTGCTATTATCGTTATGGGACATGGACATTTTGCGAGTGGGATTGTCTCATCCCTAGAGTTAATTGCCGGACAACA
AGAGAAGGTAACTGCAATTGATTTTACTACTGAGATGACCGCCGCTGATGTGCAGGACCAATTATCTCGTGCTTTAATCC
CAGAAGAGGAAACCTTAGTTCTTTGTGACCTTCTAGGAGGTACCCCTTTTAAAGTCGCTGCAACCTTAATGGAATCTCTT
CCAAACACGACGTGTAATGTTCTTTCTGGCCTTAACTTAGCCATGCTGATTGAAGCTAGTTTTGCAAGACAAACAGCAGC
TTCTTTTGACGACTTAGTATCAGGACTTATAATATGTTCTAAAGAAGGCATTGTTGACTGGAAAACTCTTTCTCAGCAAG
AAGACGGTGCGACAGACGATGAGTTAGGAGGCATTTAA

Upstream 100 bases:

>100_bases
TTTTTCTTTTTCAAAAATCTTTTTAAAAAAGTATTGACAAAGAATTTTGAAAGCGCTACAATATTTTTAAGTAAACCGGT
AAACCAAAAGGAGTTTTATT

Downstream 100 bases:

>100_bases
TGACACGACGTTTAAAAACCATTGCTCTTGAACCTATCAAGCAGCCTGAACGATTTACCAAAGAAGATTTCCTAAGCCAA
GAAGACATTACTCAGGCCCT

Product: putative PTS dependent N-acetyl-galactosamine- and galactosamine IIA component

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MIAIIVMGHGHFASGIVSSLELIAGQQEKVTAIDFTTEMTAADVQDQLSRALIPEEETLVLCDLLGGTPFKVAATLMESL
PNTTCNVLSGLNLAMLIEASFARQTAASFDDLVSGLIICSKEGIVDWKTLSQQEDGATDDELGGI

Sequences:

>Translated_145_residues
MIAIIVMGHGHFASGIVSSLELIAGQQEKVTAIDFTTEMTAADVQDQLSRALIPEEETLVLCDLLGGTPFKVAATLMESL
PNTTCNVLSGLNLAMLIEASFARQTAASFDDLVSGLIICSKEGIVDWKTLSQQEDGATDDELGGI
>Mature_145_residues
MIAIIVMGHGHFASGIVSSLELIAGQQEKVTAIDFTTEMTAADVQDQLSRALIPEEETLVLCDLLGGTPFKVAATLMESL
PNTTCNVLSGLNLAMLIEASFARQTAASFDDLVSGLIICSKEGIVDWKTLSQQEDGATDDELGGI

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=126, Percent_Identity=31.7460317460317, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR004720 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 15316; Mature: 15316

Theoretical pI: Translated: 3.82; Mature: 3.82

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAIIVMGHGHFASGIVSSLELIAGQQEKVTAIDFTTEMTAADVQDQLSRALIPEEETLV
CEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCEE
LCDLLGGTPFKVAATLMESLPNTTCNVLSGLNLAMLIEASFARQTAASFDDLVSGLIICS
EEECCCCCHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEC
KEGIVDWKTLSQQEDGATDDELGGI
CCCCCCHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIAIIVMGHGHFASGIVSSLELIAGQQEKVTAIDFTTEMTAADVQDQLSRALIPEEETLV
CEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCEE
LCDLLGGTPFKVAATLMESLPNTTCNVLSGLNLAMLIEASFARQTAASFDDLVSGLIICS
EEECCCCCHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEC
KEGIVDWKTLSQQEDGATDDELGGI
CCCCCCHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA