Click here to switch to the map view.

The map label for this gene is regR [H]

Identifier: 21909977

GI number: 21909977

Start: 485007

End: 486002

Strand: Reverse

Name: regR [H]

Synonym: SpyM3_0441

Alternate gene names: 21909977

Gene position: 486002-485007 (Counterclockwise)

Preceding gene: 21909978

Following gene: 21909974

Centisome position: 25.57

GC content: 35.64

Gene sequence:

>996_bases
ATGCAGCGCAAAGTAACCATCAAAGATATTGCTGAATTAGCGAAAACTTCAAAAACAACAGTTTCTTTCTACTTAAATGG
TAGGTTTGACAAGATGTCTGAAGAAACTAAAAACCGCATTAAAAGCACGATTGAAGCTACCAATTATAAACCAAGCATTG
CTGCCAGAAGTCTAAACGCCAAATCTACTAAACTCATTGGCGTGGTTATTGGTGATATTACCAATAGTTTTTCCAATCAA
ATTGTTAAGGGAATTGAAAGCAAAGCCCAGGAATTTGGTTACCAAATTATCATCGGAAACAGCAACTACGACCCTTCTCG
CGAAGATGAACTCATTGAAAAGATGCTGAACCTCGGAGTGGATGGCTTTATCATTCAACCAACTTCTAACTTTAGAAAAT
ATTCACGTATTATCGATATTAAAAAAAAGAAGGTTGTCTTTTTTGATAGCCAACTTTACGAACATCGCACAAACTGGGTC
AAAACTAATAATTATGATGCTGTTTATGATACCATTCAACAATGTATCGATAAAGGTTATGAACATTTTATCATGATCAC
GGGTAACCCCAATTTACTTAGCACTCGTATTGAACGCGCTTCGGGCTTCATTGATGTGTTAGAAGCTAATCATCTTACTC
ATCAAGAAATGATTATTGATGAAAATCAGACTTCTTCAGAGGCAATTGCTCAGTTTTTGCAAGGATCACTGACTAAAAAG
AGTTTGGTTTTTGTCCCCAATTGTTGGGCTCTTCCAAAAGTATTTACAGCTATGAAGTCTCTTAAATTTAATATACCAGA
GATTGGACTAGTAGGATTTGATAACATTGAGTGGACAAAATTTTCTTCACCAACACTAACAACTATTATCCAACCCGCCT
ATGAAGAAGGCGAACAGGCTACTAAGATTTTAATCGATGATATTGAAGGTCACAGTCAAGAAGCCAAACAACAAATTTTT
GACTGTCAAGTCAATTGGCAAGAATCAACTTTTTAA

Upstream 100 bases:

>100_bases
CCATATCACGAATGAGATTATTCGATTGAAACACTAAATCTTTTACGAGTCCTTATGTTATAATATGACTAACCATTACT
ATTTTAAAGGAGTGTATGCA

Downstream 100 bases:

>100_bases
TGTCAGGCATACCAATAGTCCTTATCGTTGTAACGATTATAAAAAAAGGTTTGAGACAATTGTCTCAAACCTTTTAGCGT
CTATTAAATTGCTTTTTCAT

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Kdg operon repressor [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MQRKVTIKDIAELAKTSKTTVSFYLNGRFDKMSEETKNRIKSTIEATNYKPSIAARSLNAKSTKLIGVVIGDITNSFSNQ
IVKGIESKAQEFGYQIIIGNSNYDPSREDELIEKMLNLGVDGFIIQPTSNFRKYSRIIDIKKKKVVFFDSQLYEHRTNWV
KTNNYDAVYDTIQQCIDKGYEHFIMITGNPNLLSTRIERASGFIDVLEANHLTHQEMIIDENQTSSEAIAQFLQGSLTKK
SLVFVPNCWALPKVFTAMKSLKFNIPEIGLVGFDNIEWTKFSSPTLTTIIQPAYEEGEQATKILIDDIEGHSQEAKQQIF
DCQVNWQESTF

Sequences:

>Translated_331_residues
MQRKVTIKDIAELAKTSKTTVSFYLNGRFDKMSEETKNRIKSTIEATNYKPSIAARSLNAKSTKLIGVVIGDITNSFSNQ
IVKGIESKAQEFGYQIIIGNSNYDPSREDELIEKMLNLGVDGFIIQPTSNFRKYSRIIDIKKKKVVFFDSQLYEHRTNWV
KTNNYDAVYDTIQQCIDKGYEHFIMITGNPNLLSTRIERASGFIDVLEANHLTHQEMIIDENQTSSEAIAQFLQGSLTKK
SLVFVPNCWALPKVFTAMKSLKFNIPEIGLVGFDNIEWTKFSSPTLTTIIQPAYEEGEQATKILIDDIEGHSQEAKQQIF
DCQVNWQESTF
>Mature_331_residues
MQRKVTIKDIAELAKTSKTTVSFYLNGRFDKMSEETKNRIKSTIEATNYKPSIAARSLNAKSTKLIGVVIGDITNSFSNQ
IVKGIESKAQEFGYQIIIGNSNYDPSREDELIEKMLNLGVDGFIIQPTSNFRKYSRIIDIKKKKVVFFDSQLYEHRTNWV
KTNNYDAVYDTIQQCIDKGYEHFIMITGNPNLLSTRIERASGFIDVLEANHLTHQEMIIDENQTSSEAIAQFLQGSLTKK
SLVFVPNCWALPKVFTAMKSLKFNIPEIGLVGFDNIEWTKFSSPTLTTIIQPAYEEGEQATKILIDDIEGHSQEAKQQIF
DCQVNWQESTF

Specific function: Transcriptional repressor of the kdg operon for pectin utilization [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=333, Percent_Identity=26.1261261261261, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790194, Length=311, Percent_Identity=30.2250803858521, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1787948, Length=328, Percent_Identity=26.5243902439024, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789068, Length=319, Percent_Identity=25.0783699059561, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790715, Length=326, Percent_Identity=29.7546012269939, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1789202, Length=295, Percent_Identity=23.3898305084746, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI48994940, Length=319, Percent_Identity=21.9435736677116, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1788474, Length=323, Percent_Identity=22.6006191950464, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786268, Length=204, Percent_Identity=29.9019607843137, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787906, Length=297, Percent_Identity=23.2323232323232, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787580, Length=318, Percent_Identity=24.8427672955975, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786540, Length=327, Percent_Identity=22.9357798165138, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790689, Length=330, Percent_Identity=24.5454545454545, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012310
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37686; Mature: 37686

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRKVTIKDIAELAKTSKTTVSFYLNGRFDKMSEETKNRIKSTIEATNYKPSIAARSLNA
CCCEEEHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
KSTKLIGVVIGDITNSFSNQIVKGIESKAQEFGYQIIIGNSNYDPSREDELIEKMLNLGV
CCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHCCC
DGFIIQPTSNFRKYSRIIDIKKKKVVFFDSQLYEHRTNWVKTNNYDAVYDTIQQCIDKGY
CCEEECCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCEECCCCHHHHHHHHHHHHCCC
EHFIMITGNPNLLSTRIERASGFIDVLEANHLTHQEMIIDENQTSSEAIAQFLQGSLTKK
EEEEEEECCCCHHHHHHHHHCCHHHHHHCCCCCHHHHEECCCCCCHHHHHHHHHCCCCCC
SLVFVPNCWALPKVFTAMKSLKFNIPEIGLVGFDNIEWTKFSSPTLTTIIQPAYEEGEQA
EEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCHHHHCCHHHHCCHHH
TKILIDDIEGHSQEAKQQIFDCQVNWQESTF
HHHEEECCCCCHHHHHHHHHCCEECCCCCCC
>Mature Secondary Structure
MQRKVTIKDIAELAKTSKTTVSFYLNGRFDKMSEETKNRIKSTIEATNYKPSIAARSLNA
CCCEEEHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
KSTKLIGVVIGDITNSFSNQIVKGIESKAQEFGYQIIIGNSNYDPSREDELIEKMLNLGV
CCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHCCC
DGFIIQPTSNFRKYSRIIDIKKKKVVFFDSQLYEHRTNWVKTNNYDAVYDTIQQCIDKGY
CCEEECCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCEECCCCHHHHHHHHHHHHCCC
EHFIMITGNPNLLSTRIERASGFIDVLEANHLTHQEMIIDENQTSSEAIAQFLQGSLTKK
EEEEEEECCCCHHHHHHHHHCCHHHHHHCCCCCHHHHEECCCCCCHHHHHHHHHCCCCCC
SLVFVPNCWALPKVFTAMKSLKFNIPEIGLVGFDNIEWTKFSSPTLTTIIQPAYEEGEQA
EEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCHHHHCCHHHHCCHHH
TKILIDDIEGHSQEAKQQIFDCQVNWQESTF
HHHEEECCCCCHHHHHHHHHCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377 [H]