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The map label for this gene is insF1 [C]

Identifier: 21909933

GI number: 21909933

Start: 437648

End: 438451

Strand: Reverse

Name: insF1 [C]

Synonym: SpyM3_0397

Alternate gene names: 21909933

Gene position: 438451-437648 (Counterclockwise)

Preceding gene: 21909934

Following gene: 21909927

Centisome position: 23.07

GC content: 33.71

Gene sequence:

>804_bases
TTGTGTCGTGTTTTACGTGTCAATCGCTCCACTTACTATAAATTTCTAAAACATAAGCCCTCCAAAAGAGAATTGGATAA
TCAAATTTATAAAAAACAAATACTTGAGATTTATACCAAAGCGAACAAAAGACTTGGTGTGAAGTCTATCAAAGTCATTC
TTCAAAGAGACTACGACACAAAAATCTCTGAAGGAAGAATTTACCGTCTGATGAAGAATATGGCGCTTCCTAAAATGGCT
ACCGTTAAGCCAAAAACAGCTCTTAAAAGGACTCAAAAAACGTATCCTCAAAACTTACTCAACCAGAAATTTAATCCTGA
CAAACCAAATCAAGTATGGTCTACTGACTTCACCTATATTTCTATTGGATATAAGAAATATGTCTATCTCTGCGCAATAC
TTGATCTCTATTCTAGAAAATGTATTGCTTGGAAACTGAGTCATCATATGGATGCAAAGTTAGCATGTGACACTCTAGAA
TTAGCTCTTAATAAAAGAAAGATTGAAGGAACACTTCTCTTTCATTCCGACCAAGGGTCACAATTTAAGGCCAGGGAATT
GAGAAAAATAATTGATGACAACACTATCATGCATTCTTTTTCTAAACCTGGATATCCTTATGATAATGCCGTAACGGAAG
CATTTTTCAAGTATTTAAAGCATAGACAAATCAACCGAAAAAAGTATCAAAATATCAAACAGGTTCAATTAGACTGCTTT
GAATACATTGAGAATTTTTATAACAATTACAACCCACATACGGCTAATCTAGGACTAACCCCTAATCAGAAAGAAGAAAA
TTAG

Upstream 100 bases:

>100_bases
AGAACCTTATCCTAAAAAAAGCAAGTGCCATATTCATGCAAAACTTAAAGTAAGACTCCTCGCTGTCTATCGGTTACGCT
TTGAACACGCCACAACAACC

Downstream 100 bases:

>100_bases
TTTAACGCAATAAAATAACACGGTTTTCTGTCTACTTATTTGACATTAGTCCAAAATGTACATCCTAATGGTGTCGTTTT
TTTTTTATTTCACTAGACAT

Product: transposase TnpA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MCRVLRVNRSTYYKFLKHKPSKRELDNQIYKKQILEIYTKANKRLGVKSIKVILQRDYDTKISEGRIYRLMKNMALPKMA
TVKPKTALKRTQKTYPQNLLNQKFNPDKPNQVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHHMDAKLACDTLE
LALNKRKIEGTLLFHSDQGSQFKARELRKIIDDNTIMHSFSKPGYPYDNAVTEAFFKYLKHRQINRKKYQNIKQVQLDCF
EYIENFYNNYNPHTANLGLTPNQKEEN

Sequences:

>Translated_267_residues
MCRVLRVNRSTYYKFLKHKPSKRELDNQIYKKQILEIYTKANKRLGVKSIKVILQRDYDTKISEGRIYRLMKNMALPKMA
TVKPKTALKRTQKTYPQNLLNQKFNPDKPNQVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHHMDAKLACDTLE
LALNKRKIEGTLLFHSDQGSQFKARELRKIIDDNTIMHSFSKPGYPYDNAVTEAFFKYLKHRQINRKKYQNIKQVQLDCF
EYIENFYNNYNPHTANLGLTPNQKEEN
>Mature_267_residues
MCRVLRVNRSTYYKFLKHKPSKRELDNQIYKKQILEIYTKANKRLGVKSIKVILQRDYDTKISEGRIYRLMKNMALPKMA
TVKPKTALKRTQKTYPQNLLNQKFNPDKPNQVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHHMDAKLACDTLE
LALNKRKIEGTLLFHSDQGSQFKARELRKIIDDNTIMHSFSKPGYPYDNAVTEAFFKYLKHRQINRKKYQNIKQVQLDCF
EYIENFYNNYNPHTANLGLTPNQKEEN

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=220, Percent_Identity=30.9090909090909, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI1788405, Length=274, Percent_Identity=30.6569343065693, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1787263, Length=274, Percent_Identity=30.6569343065693, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1786753, Length=274, Percent_Identity=30.6569343065693, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1786570, Length=274, Percent_Identity=30.6569343065693, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1786490, Length=274, Percent_Identity=30.6569343065693, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1789981, Length=263, Percent_Identity=30.4182509505703, Blast_Score=110, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31774; Mature: 31774

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRVLRVNRSTYYKFLKHKPSKRELDNQIYKKQILEIYTKANKRLGVKSIKVILQRDYDT
CCEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
KISEGRIYRLMKNMALPKMATVKPKTALKRTQKTYPQNLLNQKFNPDKPNQVWSTDFTYI
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCEEE
SIGYKKYVYLCAILDLYSRKCIAWKLSHHMDAKLACDTLELALNKRKIEGTLLFHSDQGS
EECHHHHHHHHHHHHHHCCHHHEEEHHHCCCHHHHHHHHHHHHHHHEEEEEEEEECCCCC
QFKARELRKIIDDNTIMHSFSKPGYPYDNAVTEAFFKYLKHRQINRKKYQNIKQVQLDCF
CHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYIENFYNNYNPHTANLGLTPNQKEEN
HHHHHHHHCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MCRVLRVNRSTYYKFLKHKPSKRELDNQIYKKQILEIYTKANKRLGVKSIKVILQRDYDT
CCEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
KISEGRIYRLMKNMALPKMATVKPKTALKRTQKTYPQNLLNQKFNPDKPNQVWSTDFTYI
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCEEE
SIGYKKYVYLCAILDLYSRKCIAWKLSHHMDAKLACDTLELALNKRKIEGTLLFHSDQGS
EECHHHHHHHHHHHHHHCCHHHEEEHHHCCCHHHHHHHHHHHHHHHEEEEEEEEECCCCC
QFKARELRKIIDDNTIMHSFSKPGYPYDNAVTEAFFKYLKHRQINRKKYQNIKQVQLDCF
CHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYIENFYNNYNPHTANLGLTPNQKEEN
HHHHHHHHCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8387149 [H]