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The map label for this gene is murE [H]

Identifier: 21909820

GI number: 21909820

Start: 321096

End: 322541

Strand: Reverse

Name: murE [H]

Synonym: SpyM3_0284

Alternate gene names: 21909820

Gene position: 322541-321096 (Counterclockwise)

Preceding gene: 21909830

Following gene: 21909819

Centisome position: 16.97

GC content: 41.36

Gene sequence:

>1446_bases
ATGATAACCATTGAACAATTATTAGACATTTTAAAAAAAGACCATAATTTTCGTGAGGTTCTTGATGCCGACGAATACCA
CTACCATTATCAAGGCTTTAGTTTTGAGCGCTTGAGTTACGATAGCCGTCAAGTAGATGGCAAGACCCTTTTTTTTGCTA
AAGGGGCAACTTTCAAAGCTGACTACCTTAAAGAAGCAATCACTAACGGATTACAACTCTATATTTCAGAGGTAGATTAC
GAGCTTGGTATTCCCGTTGTTTTGGTCACTGATATCAAAAAAGCTATGAGTCTAATTGCCATGGCTTTCTATGGCAACCC
TCAAGAAAAGCTCAAACTACTAGCTTTTACAGGTACTAAGGGAAAGACGACGGCTGCTTATTTTGCCTACCACATGTTGA
AAGAATCCTATAAACCTGCCATGTTTTCTACCATGAATACCACCTTAGATGGCAAGACCTTTTTCAAATCACAACTCACA
ACTCCTGAGAGCTTGGATCTTTTTGCTATGATGGCTGAGTGTGTGACTAATGGCATGACCCACTTGATTATGGAAGTTTC
TAGTCAGGCTTACCTCGTTGACCGTGTCTATGGGCTTACGTTCGATGTTGGGGTTTTCCTAAATATTAGTCCTGATCATA
TTGGCCCAATCGAGCATCCAACGTTTGAAGATTATTTCTATCATAAGCGTCTTTTAATGGAGAATAGCAGAGCTGTTGTG
ATCAATAGCGTGATGGATCATTTTTCATTTCTAGCGGATCAGGTAGCGGATCAAGAGCATGTGTTTTATGGTCCCTTGTC
TGACAATCAAATCACCACTAGCCAAGCTTTCTCCTTTGAAGCTAAAGGACAATTAGCTGGCCATTATGACATTCAATTAA
TTGGCCATTTTAACCAAGAAAATGCTATGGCAGCTGGACTTGCCTGCCTTCGTTTGGGTGCTAGCCTAGCTGATATTCAA
AAAGGTATCGCTAAGACTCGCGTCCCAGGCCGTATGGAAGTCCTCACTATGACAAATCATGCTAAAGTTTTTGTTGATTA
TGCCCATAATGGTGACAGCTTAGAAAAATTACTCAGCGTTGTGGAAGAACATCAGACAGGCAAATTAATGTTGATTTTAG
GTGCACCAGGAAATAAAGGCGAGAGCAGACGAGCTGATTTTGGTAGGGTGATTCATCAGCATCCAAACCTCACCGTCATT
CTCACTGCTGATGACCCTAATTTCGAGGATCCAGAAGATATTTCAAAAGAAATTGCCAGCCATATCGCACGCCCGGTAGA
GATTATTAGTGACCGTGAGCAAGCTATTCAAAAAGCCATGTCTCTTTGCCAAGGAGCAAAAGATGCTGTGATTATCGCTG
GGAAAGGCGCAGATGCTTATCAAATTGTTAAAGGACAACAAGTAGCTTATGCTGGTGATTTAGCAATCGCCAAACACTAC
CTCTAG

Upstream 100 bases:

>100_bases
TTTCTGTCGACATATCTATTCTTTCCTAGTTTCTATTAGCTTTATTATAAACTTTTGCTTGTGACTTGTAAAACAAGAAC
TTTAAGGATAAAATAAGGGT

Downstream 100 bases:

>100_bases
AGTATGTTGACGTTAGTTTAGAATGATTATCGCTATTGTTTTTAATCATTCTAAACTAATATTTCTTTTTTAATACATCC
TAAAAATCACATCAAAAAAG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--L-lysine ligase

Products: NA

Alternate protein names: L-lysine-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:L-Lys ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MITIEQLLDILKKDHNFREVLDADEYHYHYQGFSFERLSYDSRQVDGKTLFFAKGATFKADYLKEAITNGLQLYISEVDY
ELGIPVVLVTDIKKAMSLIAMAFYGNPQEKLKLLAFTGTKGKTTAAYFAYHMLKESYKPAMFSTMNTTLDGKTFFKSQLT
TPESLDLFAMMAECVTNGMTHLIMEVSSQAYLVDRVYGLTFDVGVFLNISPDHIGPIEHPTFEDYFYHKRLLMENSRAVV
INSVMDHFSFLADQVADQEHVFYGPLSDNQITTSQAFSFEAKGQLAGHYDIQLIGHFNQENAMAAGLACLRLGASLADIQ
KGIAKTRVPGRMEVLTMTNHAKVFVDYAHNGDSLEKLLSVVEEHQTGKLMLILGAPGNKGESRRADFGRVIHQHPNLTVI
LTADDPNFEDPEDISKEIASHIARPVEIISDREQAIQKAMSLCQGAKDAVIIAGKGADAYQIVKGQQVAYAGDLAIAKHY
L

Sequences:

>Translated_481_residues
MITIEQLLDILKKDHNFREVLDADEYHYHYQGFSFERLSYDSRQVDGKTLFFAKGATFKADYLKEAITNGLQLYISEVDY
ELGIPVVLVTDIKKAMSLIAMAFYGNPQEKLKLLAFTGTKGKTTAAYFAYHMLKESYKPAMFSTMNTTLDGKTFFKSQLT
TPESLDLFAMMAECVTNGMTHLIMEVSSQAYLVDRVYGLTFDVGVFLNISPDHIGPIEHPTFEDYFYHKRLLMENSRAVV
INSVMDHFSFLADQVADQEHVFYGPLSDNQITTSQAFSFEAKGQLAGHYDIQLIGHFNQENAMAAGLACLRLGASLADIQ
KGIAKTRVPGRMEVLTMTNHAKVFVDYAHNGDSLEKLLSVVEEHQTGKLMLILGAPGNKGESRRADFGRVIHQHPNLTVI
LTADDPNFEDPEDISKEIASHIARPVEIISDREQAIQKAMSLCQGAKDAVIIAGKGADAYQIVKGQQVAYAGDLAIAKHY
L
>Mature_481_residues
MITIEQLLDILKKDHNFREVLDADEYHYHYQGFSFERLSYDSRQVDGKTLFFAKGATFKADYLKEAITNGLQLYISEVDY
ELGIPVVLVTDIKKAMSLIAMAFYGNPQEKLKLLAFTGTKGKTTAAYFAYHMLKESYKPAMFSTMNTTLDGKTFFKSQLT
TPESLDLFAMMAECVTNGMTHLIMEVSSQAYLVDRVYGLTFDVGVFLNISPDHIGPIEHPTFEDYFYHKRLLMENSRAVV
INSVMDHFSFLADQVADQEHVFYGPLSDNQITTSQAFSFEAKGQLAGHYDIQLIGHFNQENAMAAGLACLRLGASLADIQ
KGIAKTRVPGRMEVLTMTNHAKVFVDYAHNGDSLEKLLSVVEEHQTGKLMLILGAPGNKGESRRADFGRVIHQHPNLTVI
LTADDPNFEDPEDISKEIASHIARPVEIISDREQAIQKAMSLCQGAKDAVIIAGKGADAYQIVKGQQVAYAGDLAIAKHY
L

Specific function: Catalyzes the addition of L-lysine to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=497, Percent_Identity=29.1750503018109, Blast_Score=143, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.7 [H]

Molecular weight: Translated: 53628; Mature: 53628

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIEQLLDILKKDHNFREVLDADEYHYHYQGFSFERLSYDSRQVDGKTLFFAKGATFKA
CCCHHHHHHHHHCCCCHHHHHCCCCCEEEECCEEEHHCCCCCCCCCCCEEEEECCCCCHH
DYLKEAITNGLQLYISEVDYELGIPVVLVTDIKKAMSLIAMAFYGNPQEKLKLLAFTGTK
HHHHHHHHCCCEEEHHHCCCCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHEEEEEEECCC
GKTTAAYFAYHMLKESYKPAMFSTMNTTLDGKTFFKSQLTTPESLDLFAMMAECVTNGMT
CCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCHH
HLIMEVSSQAYLVDRVYGLTFDVGVFLNISPDHIGPIEHPTFEDYFYHKRLLMENSRAVV
HHHHHHCCCEEEEHHHHCEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
INSVMDHFSFLADQVADQEHVFYGPLSDNQITTSQAFSFEAKGQLAGHYDIQLIGHFNQE
HHHHHHHHHHHHHHHCCCCCEEECCCCCCCEECCCCEEEECCCCCCCEEEEEEEEECCCC
NAMAAGLACLRLGASLADIQKGIAKTRVPGRMEVLTMTNHAKVFVDYAHNGDSLEKLLSV
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEEECCCCHHHHHHHHH
VEEHQTGKLMLILGAPGNKGESRRADFGRVIHQHPNLTVILTADDPNFEDPEDISKEIAS
HHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHH
HIARPVEIISDREQAIQKAMSLCQGAKDAVIIAGKGADAYQIVKGQQVAYAGDLAIAKHY
HHCCHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCEEEEECCHHHHHCC
L
C
>Mature Secondary Structure
MITIEQLLDILKKDHNFREVLDADEYHYHYQGFSFERLSYDSRQVDGKTLFFAKGATFKA
CCCHHHHHHHHHCCCCHHHHHCCCCCEEEECCEEEHHCCCCCCCCCCCEEEEECCCCCHH
DYLKEAITNGLQLYISEVDYELGIPVVLVTDIKKAMSLIAMAFYGNPQEKLKLLAFTGTK
HHHHHHHHCCCEEEHHHCCCCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHEEEEEEECCC
GKTTAAYFAYHMLKESYKPAMFSTMNTTLDGKTFFKSQLTTPESLDLFAMMAECVTNGMT
CCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCHH
HLIMEVSSQAYLVDRVYGLTFDVGVFLNISPDHIGPIEHPTFEDYFYHKRLLMENSRAVV
HHHHHHCCCEEEEHHHHCEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
INSVMDHFSFLADQVADQEHVFYGPLSDNQITTSQAFSFEAKGQLAGHYDIQLIGHFNQE
HHHHHHHHHHHHHHHCCCCCEEECCCCCCCEECCCCEEEECCCCCCCEEEEEEEEECCCC
NAMAAGLACLRLGASLADIQKGIAKTRVPGRMEVLTMTNHAKVFVDYAHNGDSLEKLLSV
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEEECCCCHHHHHHHHH
VEEHQTGKLMLILGAPGNKGESRRADFGRVIHQHPNLTVILTADDPNFEDPEDISKEIAS
HHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHH
HIARPVEIISDREQAIQKAMSLCQGAKDAVIIAGKGADAYQIVKGQQVAYAGDLAIAKHY
HHCCHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCEEEEECCHHHHHCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]