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The map label for this gene is yhjJ [H]

Identifier: 218930986

GI number: 218930986

Start: 4496486

End: 4497985

Strand: Reverse

Name: yhjJ [H]

Synonym: YPO3991

Alternate gene names: 218930986

Gene position: 4497985-4496486 (Counterclockwise)

Preceding gene: 218930987

Following gene: 218930983

Centisome position: 96.65

GC content: 51.07

Gene sequence:

>1500_bases
ATGCAGGGCACCAAAATTCGTCTTATGGTTGGTGGGTTATTATTGGCGGCGGCCAGCAATAATGTGCAGGCTGAAGCACT
ACAACCCGACCCTGCCTGGCAGCAGGGAAAGTTAGATAATGGGTTTTCATGGCAGTTGCTGGCAACGCCGCAGCGCCCAA
GTGATCGGATCGAGCTACGCTTGATCGTCAATACCGGATCATTATCCGAAAATACACAGGAAGTGGGCTTTGCCCATTTA
CTGCCTCGTCTCGCATTAATGAGCAGCGCGAGTTTTACCCCCGCCCAACTCCAGTCGCTATGGCAGCAAGGGATCGATAA
CGAACGCCCATTGCCACCGGCTATTACATCGTATGATTTCACCTTATACAGCCTGAGCTTACCCAATAACCGTCCTGATC
TGCTGAAGGATGCGTTGGCATGGTTATCTGATACGGCGGGTAATCTGGCGGTAAGTGAGCAAACGGTTAATGCAGCCTTA
AATACGGCAACCGACCCCATTGCGACTTTCCCACAAAATATTCAGGAACCGTGGTGGCGTTATCGCCTCAAAGGTTCTTC
TTTAATCGGCCACGATCCCGGTCAGCCAGTGACGCAACCGGTCGATGTAGAGAAGCTAAAGCAGTTTTATCAGCAATGGT
ATACCCCAGATGCGATGACGCTGTATGTGGTCGGTAATGTTGATAGCCGTAGTATCGCCGCCCAAATCAGTAAAGCGTTT
TCTGAGCTGAAAGGTAAGCGTACCGCGCCAGCGGCAGTTGCAACCTTAGCCCCGCTACCACCAGAACCGGTGAGTTTGAT
GAATGAACAGGCGGCACAGGACACGTTATCGTTGATGTGGGATACCCCATGGCACCCCATTCAAGACTCTATGGCCCTGA
GCCGCTATTGGCGCAGCGATTTAGCCCGTGAAGCGCTGTTTTGGCATATCAAGCAGGTGTTGGAGAAAAATAATCAGAAG
AACCTAAAGCTGGGCTTTGATTGCCGGGTCCAGTATCAACGCGCCCAATGTGCTATTCACTTAAATACCCCCGTTGAGAA
CCTGACAGCCAATATGACATTTGTTGCCCGTGAGTTGGCGGCATTGCGTGCTAACGGCCTGAGCCAGGCTGAGTTTGATG
CGTTGATGACACAGAAAAACGACCAACTCAGTAAGTTGTTCGCCACCTATGCGCGTACTGATACCGATATTTTGATGAGC
CAACGTCTGCGCTCACAGCAAAGTGGTGTTGTGGACATCGCGCCGGAACAGTATCAAAAATTGCGGCAGGCATTCTTGTC
TGGGTTGACGCTGGCAGAGCTGAATCGGGAGTTAAAACAGCAACTTTCACAAGATACCACCTTGGTTCTGATGCAACCGA
AAGGTGAACCTGAAGTTAATGTGAAGGCGTTGCAGGAGATCTATAACGGCATTATGGCACCACAGACGGTGGCGGAAGAA
GAGGTTGCCCCTGCTGAAGCGGTAGAAACTGCACCTGTTATGCCGACAACCGCGCAATAA

Upstream 100 bases:

>100_bases
CAAGTCGGTGTAACCACCCAATGCAGGGTGTTGGCAGGATGAAATAACGTTAAAAATTGCATTATTTAAAAATTGGATAG
TTTATTCAGTAGGGGTTCAC

Downstream 100 bases:

>100_bases
CGAACGAATGACGAATAAATAATGAACCTGGAGCGTTGTTGGGGCGGTTAACACCGGGCTAAGGCCACGTTAGCCAGGCG
TAAAATGACCGATGGCTGTA

Product: putative insulinase family protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MQGTKIRLMVGGLLLAAASNNVQAEALQPDPAWQQGKLDNGFSWQLLATPQRPSDRIELRLIVNTGSLSENTQEVGFAHL
LPRLALMSSASFTPAQLQSLWQQGIDNERPLPPAITSYDFTLYSLSLPNNRPDLLKDALAWLSDTAGNLAVSEQTVNAAL
NTATDPIATFPQNIQEPWWRYRLKGSSLIGHDPGQPVTQPVDVEKLKQFYQQWYTPDAMTLYVVGNVDSRSIAAQISKAF
SELKGKRTAPAAVATLAPLPPEPVSLMNEQAAQDTLSLMWDTPWHPIQDSMALSRYWRSDLAREALFWHIKQVLEKNNQK
NLKLGFDCRVQYQRAQCAIHLNTPVENLTANMTFVARELAALRANGLSQAEFDALMTQKNDQLSKLFATYARTDTDILMS
QRLRSQQSGVVDIAPEQYQKLRQAFLSGLTLAELNRELKQQLSQDTTLVLMQPKGEPEVNVKALQEIYNGIMAPQTVAEE
EVAPAEAVETAPVMPTTAQ

Sequences:

>Translated_499_residues
MQGTKIRLMVGGLLLAAASNNVQAEALQPDPAWQQGKLDNGFSWQLLATPQRPSDRIELRLIVNTGSLSENTQEVGFAHL
LPRLALMSSASFTPAQLQSLWQQGIDNERPLPPAITSYDFTLYSLSLPNNRPDLLKDALAWLSDTAGNLAVSEQTVNAAL
NTATDPIATFPQNIQEPWWRYRLKGSSLIGHDPGQPVTQPVDVEKLKQFYQQWYTPDAMTLYVVGNVDSRSIAAQISKAF
SELKGKRTAPAAVATLAPLPPEPVSLMNEQAAQDTLSLMWDTPWHPIQDSMALSRYWRSDLAREALFWHIKQVLEKNNQK
NLKLGFDCRVQYQRAQCAIHLNTPVENLTANMTFVARELAALRANGLSQAEFDALMTQKNDQLSKLFATYARTDTDILMS
QRLRSQQSGVVDIAPEQYQKLRQAFLSGLTLAELNRELKQQLSQDTTLVLMQPKGEPEVNVKALQEIYNGIMAPQTVAEE
EVAPAEAVETAPVMPTTAQ
>Mature_499_residues
MQGTKIRLMVGGLLLAAASNNVQAEALQPDPAWQQGKLDNGFSWQLLATPQRPSDRIELRLIVNTGSLSENTQEVGFAHL
LPRLALMSSASFTPAQLQSLWQQGIDNERPLPPAITSYDFTLYSLSLPNNRPDLLKDALAWLSDTAGNLAVSEQTVNAAL
NTATDPIATFPQNIQEPWWRYRLKGSSLIGHDPGQPVTQPVDVEKLKQFYQQWYTPDAMTLYVVGNVDSRSIAAQISKAF
SELKGKRTAPAAVATLAPLPPEPVSLMNEQAAQDTLSLMWDTPWHPIQDSMALSRYWRSDLAREALFWHIKQVLEKNNQK
NLKLGFDCRVQYQRAQCAIHLNTPVENLTANMTFVARELAALRANGLSQAEFDALMTQKNDQLSKLFATYARTDTDILMS
QRLRSQQSGVVDIAPEQYQKLRQAFLSGLTLAELNRELKQQLSQDTTLVLMQPKGEPEVNVKALQEIYNGIMAPQTVAEE
EVAPAEAVETAPVMPTTAQ

Specific function: Unknown

COG id: COG0612

COG function: function code R; Predicted Zn-dependent peptidases

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M16 family [H]

Homologues:

Organism=Escherichia coli, GI1789946, Length=474, Percent_Identity=62.2362869198312, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI1787770, Length=432, Percent_Identity=22.4537037037037, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011249
- InterPro:   IPR011237
- InterPro:   IPR011765
- InterPro:   IPR007863 [H]

Pfam domain/function: PF00675 Peptidase_M16; PF05193 Peptidase_M16_C [H]

EC number: NA

Molecular weight: Translated: 55446; Mature: 55446

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGTKIRLMVGGLLLAAASNNVQAEALQPDPAWQQGKLDNGFSWQLLATPQRPSDRIELR
CCCCEEEEEEHHHHEEECCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEE
LIVNTGSLSENTQEVGFAHLLPRLALMSSASFTPAQLQSLWQQGIDNERPLPPAITSYDF
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCE
TLYSLSLPNNRPDLLKDALAWLSDTAGNLAVSEQTVNAALNTATDPIATFPQNIQEPWWR
EEEEEECCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCHHHCCHHHHCHHHH
YRLKGSSLIGHDPGQPVTQPVDVEKLKQFYQQWYTPDAMTLYVVGNVDSRSIAAQISKAF
EEECCCCEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHH
SELKGKRTAPAAVATLAPLPPEPVSLMNEQAAQDTLSLMWDTPWHPIQDSMALSRYWRSD
HHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LAREALFWHIKQVLEKNNQKNLKLGFDCRVQYQRAQCAIHLNTPVENLTANMTFVARELA
HHHHHHHHHHHHHHHCCCCCCEEEECEEEEEEEECEEEEEECCCHHHHHHHHHHHHHHHH
ALRANGLSQAEFDALMTQKNDQLSKLFATYARTDTDILMSQRLRSQQSGVVDIAPEQYQK
HHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEECHHHHHH
LRQAFLSGLTLAELNRELKQQLSQDTTLVLMQPKGEPEVNVKALQEIYNGIMAPQTVAEE
HHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHH
EVAPAEAVETAPVMPTTAQ
HCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQGTKIRLMVGGLLLAAASNNVQAEALQPDPAWQQGKLDNGFSWQLLATPQRPSDRIELR
CCCCEEEEEEHHHHEEECCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEE
LIVNTGSLSENTQEVGFAHLLPRLALMSSASFTPAQLQSLWQQGIDNERPLPPAITSYDF
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCE
TLYSLSLPNNRPDLLKDALAWLSDTAGNLAVSEQTVNAALNTATDPIATFPQNIQEPWWR
EEEEEECCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCHHHCCHHHHCHHHH
YRLKGSSLIGHDPGQPVTQPVDVEKLKQFYQQWYTPDAMTLYVVGNVDSRSIAAQISKAF
EEECCCCEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHH
SELKGKRTAPAAVATLAPLPPEPVSLMNEQAAQDTLSLMWDTPWHPIQDSMALSRYWRSD
HHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LAREALFWHIKQVLEKNNQKNLKLGFDCRVQYQRAQCAIHLNTPVENLTANMTFVARELA
HHHHHHHHHHHHHHHCCCCCCEEEECEEEEEEEECEEEEEECCCHHHHHHHHHHHHHHHH
ALRANGLSQAEFDALMTQKNDQLSKLFATYARTDTDILMSQRLRSQQSGVVDIAPEQYQK
HHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEECHHHHHH
LRQAFLSGLTLAELNRELKQQLSQDTTLVLMQPKGEPEVNVKALQEIYNGIMAPQTVAEE
HHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHH
EVAPAEAVETAPVMPTTAQ
HCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 9298646 [H]