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The map label for this gene is rbsB [H]

Identifier: 218930958

GI number: 218930958

Start: 4458896

End: 4459840

Strand: Reverse

Name: rbsB [H]

Synonym: YPO3962

Alternate gene names: 218930958

Gene position: 4459840-4458896 (Counterclockwise)

Preceding gene: 218930959

Following gene: 218930957

Centisome position: 95.83

GC content: 51.11

Gene sequence:

>945_bases
ATGCGTTTGAAACCCATAATGACCGCACTAATCGCGGGGGCCATGATAGCCAGTTCACCGGCGCTCCAGGCGAAAGAGTT
GAACTCGATAGGCGTAACGGTGGGCGATCTTGCCAACCCATTTTTCGTGCAAATCACCAAAGGTGTGGAGTTAGAAGCGC
GTAAGTTGGCCGGCGATAAAGTCAAAGTTACGCTCGTTTCCAGCGGCTACGATCTCGGCCAGCAGGTGGCACAGATAGAT
AACTTTATTGCCGCCAAAGTGGACATGATCATTCTCAATGCCGCGGATTCTAAAGGGATCGGTCCGGCGGTGAAGCGTGC
TAAAGATGCCGGTATTGTCGTGGTGGCAGTGGATGTGGCAGCAGAAGGGGCTGACGCAACGATTACCTCTGACAACACTC
AGGCCGGTGCGATGGCGTGTAAGTACATATCCGACCGTCTGAAAGAGAAAGGCAACGTGGTTATTATCAACGGGCCGCCA
GTGTCTGCCATACAAAACCGTGTTGAAGGTTGTGAAAGCGAATTCAAAAAATATCCTGATATCAAGGTCCTTTCTTCTAA
CCAAAATGCTAAGGGCAGTCGAGAAGGTGGCCTGGAGGTGATGACCTCCTTGCTGGCGGTGAACCCGAAGATCGATGGCG
TGTTTGCCATCAATGATCCAACGGCGATTGGGGCTGATTTGGCTGCCAAACAGGCACAACGTAATGAATTCTTTATTGTT
GGTGTGGACGGCAGCCCGGATGCAGAAGAAGCGCTGAAACGGGGTGGTAACACATTGTTTGTCGCGACCCCAGCGCAAGA
TCCTCAGGTCATGGCCTCCAAAGCCGTTGAGGTTGGTTATGGCATCTTGCAGGGCAACCCAGCACCAAAAGATCCCATTT
TGATACCAGTGACCCTGATCGACAAAAATAATATCAGCACTTACAAAGGCTGGACGATGAAATAA

Upstream 100 bases:

>100_bases
GATCAAAGGGGCGGTGATCATCATAGCGGTGCTGATAGACAAATACCGTACCCGTCACCATAACTCATAACTAAAAATAC
CCTTACACAGGAGTAACAGC

Downstream 100 bases:

>100_bases
CAGCTCGCCTCACTTCGGCTCGGTCTAATCCTCTCTCCCTATTAAGGAGAGGGGCAACTTTTTGAGGAGATTTTATGAAA
CGCTCCGAGGTCAACCAAAT

Product: sugar-binding transport protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MRLKPIMTALIAGAMIASSPALQAKELNSIGVTVGDLANPFFVQITKGVELEARKLAGDKVKVTLVSSGYDLGQQVAQID
NFIAAKVDMIILNAADSKGIGPAVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGAMACKYISDRLKEKGNVVIINGPP
VSAIQNRVEGCESEFKKYPDIKVLSSNQNAKGSREGGLEVMTSLLAVNPKIDGVFAINDPTAIGADLAAKQAQRNEFFIV
GVDGSPDAEEALKRGGNTLFVATPAQDPQVMASKAVEVGYGILQGNPAPKDPILIPVTLIDKNNISTYKGWTMK

Sequences:

>Translated_314_residues
MRLKPIMTALIAGAMIASSPALQAKELNSIGVTVGDLANPFFVQITKGVELEARKLAGDKVKVTLVSSGYDLGQQVAQID
NFIAAKVDMIILNAADSKGIGPAVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGAMACKYISDRLKEKGNVVIINGPP
VSAIQNRVEGCESEFKKYPDIKVLSSNQNAKGSREGGLEVMTSLLAVNPKIDGVFAINDPTAIGADLAAKQAQRNEFFIV
GVDGSPDAEEALKRGGNTLFVATPAQDPQVMASKAVEVGYGILQGNPAPKDPILIPVTLIDKNNISTYKGWTMK
>Mature_314_residues
MRLKPIMTALIAGAMIASSPALQAKELNSIGVTVGDLANPFFVQITKGVELEARKLAGDKVKVTLVSSGYDLGQQVAQID
NFIAAKVDMIILNAADSKGIGPAVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGAMACKYISDRLKEKGNVVIINGPP
VSAIQNRVEGCESEFKKYPDIKVLSSNQNAKGSREGGLEVMTSLLAVNPKIDGVFAINDPTAIGADLAAKQAQRNEFFIV
GVDGSPDAEEALKRGGNTLFVATPAQDPQVMASKAVEVGYGILQGNPAPKDPILIPVTLIDKNNISTYKGWTMK

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=288, Percent_Identity=31.5972222222222, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790526, Length=274, Percent_Identity=31.3868613138686, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790715, Length=281, Percent_Identity=25.9786476868327, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790674, Length=194, Percent_Identity=29.8969072164948, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32876; Mature: 32876

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLKPIMTALIAGAMIASSPALQAKELNSIGVTVGDLANPFFVQITKGVELEARKLAGDK
CCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEHHHCCCCEEEEEECCCCEEHHHCCCCE
VKVTLVSSGYDLGQQVAQIDNFIAAKVDMIILNAADSKGIGPAVKRAKDAGIVVVAVDVA
EEEEEEECCCHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCHHHHHHCCCCEEEEEEEEE
AEGADATITSDNTQAGAMACKYISDRLKEKGNVVIINGPPVSAIQNRVEGCESEFKKYPD
CCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCC
IKVLSSNQNAKGSREGGLEVMTSLLAVNPKIDGVFAINDPTAIGADLAAKQAQRNEFFIV
EEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEE
GVDGSPDAEEALKRGGNTLFVATPAQDPQVMASKAVEVGYGILQGNPAPKDPILIPVTLI
EECCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCEEEEECCCCCCCCEEEEEEEE
DKNNISTYKGWTMK
ECCCCCCCCCCCCC
>Mature Secondary Structure
MRLKPIMTALIAGAMIASSPALQAKELNSIGVTVGDLANPFFVQITKGVELEARKLAGDK
CCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEHHHCCCCEEEEEECCCCEEHHHCCCCE
VKVTLVSSGYDLGQQVAQIDNFIAAKVDMIILNAADSKGIGPAVKRAKDAGIVVVAVDVA
EEEEEEECCCHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCHHHHHHCCCCEEEEEEEEE
AEGADATITSDNTQAGAMACKYISDRLKEKGNVVIINGPPVSAIQNRVEGCESEFKKYPD
CCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCC
IKVLSSNQNAKGSREGGLEVMTSLLAVNPKIDGVFAINDPTAIGADLAAKQAQRNEFFIV
EEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEE
GVDGSPDAEEALKRGGNTLFVATPAQDPQVMASKAVEVGYGILQGNPAPKDPILIPVTLI
EECCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCEEEEECCCCCCCCEEEEEEEE
DKNNISTYKGWTMK
ECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]