Click here to switch to the map view.

The map label for this gene is tusA [H]

Identifier: 218930824

GI number: 218930824

Start: 4287625

End: 4287879

Strand: Reverse

Name: tusA [H]

Synonym: YPO3821

Alternate gene names: 218930824

Gene position: 4287879-4287625 (Counterclockwise)

Preceding gene: 218930827

Following gene: 218930821

Centisome position: 92.14

GC content: 51.37

Gene sequence:

>255_bases
ATGACCGATATTTTTGCTAATCCAGATAAAACCCTTGATGCTCTGGGCCTGCGTTGCCCAGAGCCGGTGATGATGGTTCG
TAAGACGGTCCGGCACATGGAAGAGGGCCAAACCCTACTGATTATTGCTGATGATCCCGCGACCACCCGTGATATTCCGG
GCTTCTGTCGCTTTATGGATCACCAGCTACTGGCGCAAGATACTGAACAGACCCCCTATCGCTATTTGGTGAGGAAGGGA
ATAACCGCAGGTTAA

Upstream 100 bases:

>100_bases
TAAACTGGCCGCCTTTGTAAGCTTGCTGACATTTTATCTTTCTATTAAACCGCTTACTGACGAATTATTAAACCGTTTAC
TGACGAACAAGAGAACCGCC

Downstream 100 bases:

>100_bases
TCCAGTATCTTGCTGATCAAGCATCGATTTAATCAAACATTAGCCTATTCAAACATTGACCTAATCAAACATCTCGGACC
TAATCAAACATCTCGCTTGC

Product: sulfur transfer protein SirA

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein A [H]

Number of amino acids: Translated: 84; Mature: 83

Protein sequence:

>84_residues
MTDIFANPDKTLDALGLRCPEPVMMVRKTVRHMEEGQTLLIIADDPATTRDIPGFCRFMDHQLLAQDTEQTPYRYLVRKG
ITAG

Sequences:

>Translated_84_residues
MTDIFANPDKTLDALGLRCPEPVMMVRKTVRHMEEGQTLLIIADDPATTRDIPGFCRFMDHQLLAQDTEQTPYRYLVRKG
ITAG
>Mature_83_residues
TDIFANPDKTLDALGLRCPEPVMMVRKTVRHMEEGQTLLIIADDPATTRDIPGFCRFMDHQLLAQDTEQTPYRYLVRKGI
TAG

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Interacts with iscS and stimulates its activity. Then, accepts a sulfur from iscS and transfers it in turn to tusD [H]

COG id: COG0425

COG function: function code O; Predicted redox protein, regulator of disulfide bond formation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0033 family. TusA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789881, Length=80, Percent_Identity=77.5, Blast_Score=137, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001455
- InterPro:   IPR022931 [H]

Pfam domain/function: PF01206 SirA [H]

EC number: NA

Molecular weight: Translated: 9536; Mature: 9405

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS01148 UPF0033

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIFANPDKTLDALGLRCPEPVMMVRKTVRHMEEGQTLLIIADDPATTRDIPGFCRFMD
CCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHH
HQLLAQDTEQTPYRYLVRKGITAG
HHHHHCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TDIFANPDKTLDALGLRCPEPVMMVRKTVRHMEEGQTLLIIADDPATTRDIPGFCRFMD
CCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHH
HQLLAQDTEQTPYRYLVRKGITAG
HHHHHCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA