Click here to switch to the map view.

The map label for this gene is thiH [H]

Identifier: 218930748

GI number: 218930748

Start: 4195117

End: 4196247

Strand: Direct

Name: thiH [H]

Synonym: YPO3743

Alternate gene names: 218930748

Gene position: 4195117-4196247 (Clockwise)

Preceding gene: 218930747

Following gene: 218930750

Centisome position: 90.15

GC content: 50.13

Gene sequence:

>1131_bases
ATGTCTGAAGACTTCAACCAGCGCTGGCAGCAGTTGGACTGGGACGATATCTCCTTAACCATCAACAGTAAAAAACCCGC
AGACGTTGAACGGGCGCTGAATGCCATCAAGCCCACCCGCGAAGATTTGATGGCACTCATTTCCCCTGCCGCACTGGCTT
ACCTGGAGCCAATGGCACAGAAGGCCCAACAACTGACACGCCAACGTTTTGGAAATACAGTCAGTTTTTATGTGCCGCTT
TATCTCTCCAATTTGTGTGCTAATGATTGCACTTACTGCGGCTTCTCGATGAGTAATCGCATCAAACGCAAGACATTGGA
TGAAGCAGAGATTATCCGAGAGTGTGAAGCCATCAAAGCGCTGGGTTTTGAGCACTTGCTGCTGGTCACTGGGGAGCACC
AAACAAAAGTAGGAATGGACTATTTTCGCCGCCACCTCCCTACGATCCGCAGTAGATTCAGTTCGCTAATGATGGAGGTT
CAGCCATTGGCAGAAGATGAATATACTGAATTAAAGGCGCTGGGATTAGATGGCGTGATGGTTTATCAGGAAACCTATCA
CCCAGCGACGTATCAGCAGCACCATTTGCGGGGTCATAAGCAGGATTTTCACTGGCGGTTGGCAACCCCAGATCGTTTGG
GCCGTGCGGGGATCGACAAGATCGGATTAGGTGCCTTGATTGGCTTGTCCAATAGTTGGCGTACCGACTGTTATATGCTG
GCGGAGCATCTGTTTTATTTGCAACAAACTTACTGGCAAACCCGTTATTCGATCTCTTTCCCTCGCTTGCGTCCGTGCGC
TGGGGGGATCGAACCCGCATCCATCATGAGTGAGCCACAACTGCTGCAACTGATCTGCGCTTTTCGCTTATTTGCACCTG
ATGTGGAACTGTCGTTATCTACTCGAGAATCGCCTTTCTTCCGCGATAATGTCATTCCGGTTGCTATCAATAATGTCAGT
GCCGGGTCAAAAACCCAACCGGGGGGTTATGCCGATGATCATCCCGAACTGGAACAATTTGCGCCCCATGATAACCGCTC
CCCGGAACAGGTCGCACAAGCGTTAACAAAAGCAGGCTTACAGCCCGTGTGGAAAGATTGGGATAGCCATTTAGGCCGTT
CATTGCGATAA

Upstream 100 bases:

>100_bases
GCTTCACCGTTCAATCCTTCACAACCTGATACATTACAACTACGGGCCACCGCCACCAGCCCGCTAACGGGTTTCCTCAG
CCAACTTGAGGAGCAAGACC

Downstream 100 bases:

>100_bases
GCGAGTTCACGTAATATTGAAAAACCGCCCGAAGGCGTAATATCAGTGAGTTACACCACTGGCTGGCATGACACTCTTGG
CGGTTTGTTTTAACCAGAGA

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MSEDFNQRWQQLDWDDISLTINSKKPADVERALNAIKPTREDLMALISPAALAYLEPMAQKAQQLTRQRFGNTVSFYVPL
YLSNLCANDCTYCGFSMSNRIKRKTLDEAEIIRECEAIKALGFEHLLLVTGEHQTKVGMDYFRRHLPTIRSRFSSLMMEV
QPLAEDEYTELKALGLDGVMVYQETYHPATYQQHHLRGHKQDFHWRLATPDRLGRAGIDKIGLGALIGLSNSWRTDCYML
AEHLFYLQQTYWQTRYSISFPRLRPCAGGIEPASIMSEPQLLQLICAFRLFAPDVELSLSTRESPFFRDNVIPVAINNVS
AGSKTQPGGYADDHPELEQFAPHDNRSPEQVAQALTKAGLQPVWKDWDSHLGRSLR

Sequences:

>Translated_376_residues
MSEDFNQRWQQLDWDDISLTINSKKPADVERALNAIKPTREDLMALISPAALAYLEPMAQKAQQLTRQRFGNTVSFYVPL
YLSNLCANDCTYCGFSMSNRIKRKTLDEAEIIRECEAIKALGFEHLLLVTGEHQTKVGMDYFRRHLPTIRSRFSSLMMEV
QPLAEDEYTELKALGLDGVMVYQETYHPATYQQHHLRGHKQDFHWRLATPDRLGRAGIDKIGLGALIGLSNSWRTDCYML
AEHLFYLQQTYWQTRYSISFPRLRPCAGGIEPASIMSEPQLLQLICAFRLFAPDVELSLSTRESPFFRDNVIPVAINNVS
AGSKTQPGGYADDHPELEQFAPHDNRSPEQVAQALTKAGLQPVWKDWDSHLGRSLR
>Mature_375_residues
SEDFNQRWQQLDWDDISLTINSKKPADVERALNAIKPTREDLMALISPAALAYLEPMAQKAQQLTRQRFGNTVSFYVPLY
LSNLCANDCTYCGFSMSNRIKRKTLDEAEIIRECEAIKALGFEHLLLVTGEHQTKVGMDYFRRHLPTIRSRFSSLMMEVQ
PLAEDEYTELKALGLDGVMVYQETYHPATYQQHHLRGHKQDFHWRLATPDRLGRAGIDKIGLGALIGLSNSWRTDCYMLA
EHLFYLQQTYWQTRYSISFPRLRPCAGGIEPASIMSEPQLLQLICAFRLFAPDVELSLSTRESPFFRDNVIPVAINNVSA
GSKTQPGGYADDHPELEQFAPHDNRSPEQVAQALTKAGLQPVWKDWDSHLGRSLR

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=370, Percent_Identity=79.7297297297297, Blast_Score=632, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42939; Mature: 42808

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDFNQRWQQLDWDDISLTINSKKPADVERALNAIKPTREDLMALISPAALAYLEPMAQ
CCCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
KAQQLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKRKTLDEAEIIRECEAIKA
HHHHHHHHHHCCEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
LGFEHLLLVTGEHQTKVGMDYFRRHLPTIRSRFSSLMMEVQPLAEDEYTELKALGLDGVM
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEE
VYQETYHPATYQQHHLRGHKQDFHWRLATPDRLGRAGIDKIGLGALIGLSNSWRTDCYML
EEECCCCCCHHHHHHHCCCCCCCEEEECCHHHHCCCCCCHHCCHHHEECCCCCCHHHHHH
AEHLFYLQQTYWQTRYSISFPRLRPCAGGIEPASIMSEPQLLQLICAFRLFAPDVELSLS
HHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCEEEEC
TRESPFFRDNVIPVAINNVSAGSKTQPGGYADDHPELEQFAPHDNRSPEQVAQALTKAGL
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHCC
QPVWKDWDSHLGRSLR
CHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SEDFNQRWQQLDWDDISLTINSKKPADVERALNAIKPTREDLMALISPAALAYLEPMAQ
CCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
KAQQLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKRKTLDEAEIIRECEAIKA
HHHHHHHHHHCCEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
LGFEHLLLVTGEHQTKVGMDYFRRHLPTIRSRFSSLMMEVQPLAEDEYTELKALGLDGVM
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEE
VYQETYHPATYQQHHLRGHKQDFHWRLATPDRLGRAGIDKIGLGALIGLSNSWRTDCYML
EEECCCCCCHHHHHHHCCCCCCCEEEECCHHHHCCCCCCHHCCHHHEECCCCCCHHHHHH
AEHLFYLQQTYWQTRYSISFPRLRPCAGGIEPASIMSEPQLLQLICAFRLFAPDVELSLS
HHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCEEEEC
TRESPFFRDNVIPVAINNVSAGSKTQPGGYADDHPELEQFAPHDNRSPEQVAQALTKAGL
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHCC
QPVWKDWDSHLGRSLR
CHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 10082377 [H]