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The map label for this gene is nfi [H]

Identifier: 218930738

GI number: 218930738

Start: 4186004

End: 4186708

Strand: Reverse

Name: nfi [H]

Synonym: YPO3733

Alternate gene names: 218930738

Gene position: 4186708-4186004 (Counterclockwise)

Preceding gene: 218930739

Following gene: 218930737

Centisome position: 89.96

GC content: 52.62

Gene sequence:

>705_bases
ATGTTTGATACTAAAGCGCTACAGGCAGAGCAGCGGCAACGGGCATCGGAGATTAGTCTTCATGATGGCATTGATAACCA
GTCTGTGCGCTTTATTGCTGGAGCTGATGTCGGTTTTGAGCAGCATGGGGAGATAACCCGTGCCGCTATCGCTATTTTAC
GCTATCCCTCTCTGGCGCTGGTGGAATACCAGGTTGCCAGAGTGGCAACTTCTCTGCCTTATATCCCCGGTTTACTCTCT
TTTCGTGAATATCCAGCGCTGTTAGCCGCGTGGGCGCAGTTGCAGCAACGCCCTGATCTGATTCTGGTCGATGGGCAAGG
CATCGCTCATCCTCGCCGTCTCGGCGTTGCCAGCCATTTTGGGTTATTGGTCGATGTACCGACGATTGGTGTCGCTAAAA
GCCGCCTGTGTGGTGATTTCTTGCCACTGCATCAAGATGTTGGTGCGGTGCAGCCCTTGTTCGATAACGATGAGCAGCTT
GGTTGGGTATGGCGCAGTAAAATCCGCTGTAACCCACTGTTTATTTCGCCTGGGCATCGGGTGAGTGTTGGCAGTGCGCT
GGCATGGGTTCAACGTTGCATGGCGGGTTATCGTCTGCCTGAACCAACCCGCTGGGCGGATGCTATTGCTTCAAACCGTC
CGCAATTTCAACGTTGGCTGCGGAAAAATCCTGATTTTCTTGGCAAGCGTAGGGATATGATTTAA

Upstream 100 bases:

>100_bases
TACATGCGCTATCACGGCCTTATCATCAAAAATAATCAGCGTGTAGGATGGAGCCGATTGGTCAATGGTGAAGACTAAAT
CGAGGGTTGAGACTAAATCA

Downstream 100 bases:

>100_bases
TTTCGGTTACACTGCCGCGCAGATAATGAATAATGAGAACTCATCATGTTACGTAATCCGATTCATCTACGGCTCGAAAA
GCTGGAAAGTTGGCAACATC

Product: endonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MFDTKALQAEQRQRASEISLHDGIDNQSVRFIAGADVGFEQHGEITRAAIAILRYPSLALVEYQVARVATSLPYIPGLLS
FREYPALLAAWAQLQQRPDLILVDGQGIAHPRRLGVASHFGLLVDVPTIGVAKSRLCGDFLPLHQDVGAVQPLFDNDEQL
GWVWRSKIRCNPLFISPGHRVSVGSALAWVQRCMAGYRLPEPTRWADAIASNRPQFQRWLRKNPDFLGKRRDMI

Sequences:

>Translated_234_residues
MFDTKALQAEQRQRASEISLHDGIDNQSVRFIAGADVGFEQHGEITRAAIAILRYPSLALVEYQVARVATSLPYIPGLLS
FREYPALLAAWAQLQQRPDLILVDGQGIAHPRRLGVASHFGLLVDVPTIGVAKSRLCGDFLPLHQDVGAVQPLFDNDEQL
GWVWRSKIRCNPLFISPGHRVSVGSALAWVQRCMAGYRLPEPTRWADAIASNRPQFQRWLRKNPDFLGKRRDMI
>Mature_234_residues
MFDTKALQAEQRQRASEISLHDGIDNQSVRFIAGADVGFEQHGEITRAAIAILRYPSLALVEYQVARVATSLPYIPGLLS
FREYPALLAAWAQLQQRPDLILVDGQGIAHPRRLGVASHFGLLVDVPTIGVAKSRLCGDFLPLHQDVGAVQPLFDNDEQL
GWVWRSKIRCNPLFISPGHRVSVGSALAWVQRCMAGYRLPEPTRWADAIASNRPQFQRWLRKNPDFLGKRRDMI

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair [H]

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family [H]

Homologues:

Organism=Homo sapiens, GI31542775, Length=195, Percent_Identity=38.4615384615385, Blast_Score=110, Evalue=9e-25,
Organism=Homo sapiens, GI257467548, Length=121, Percent_Identity=41.3223140495868, Blast_Score=74, Evalue=8e-14,
Organism=Homo sapiens, GI257467550, Length=121, Percent_Identity=42.1487603305785, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI87082357, Length=223, Percent_Identity=69.0582959641256, Blast_Score=325, Evalue=1e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007581 [H]

Pfam domain/function: PF04493 Endonuclease_5 [H]

EC number: =3.1.21.7 [H]

Molecular weight: Translated: 26245; Mature: 26245

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDTKALQAEQRQRASEISLHDGIDNQSVRFIAGADVGFEQHGEITRAAIAILRYPSLAL
CCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHCCHHHHHHHHHHHCCCHHH
VEYQVARVATSLPYIPGLLSFREYPALLAAWAQLQQRPDLILVDGQGIAHPRRLGVASHF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHCHHHHC
GLLVDVPTIGVAKSRLCGDFLPLHQDVGAVQPLFDNDEQLGWVWRSKIRCNPLFISPGHR
CEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCC
VSVGSALAWVQRCMAGYRLPEPTRWADAIASNRPQFQRWLRKNPDFLGKRRDMI
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHCCCCHHHHHCCCC
>Mature Secondary Structure
MFDTKALQAEQRQRASEISLHDGIDNQSVRFIAGADVGFEQHGEITRAAIAILRYPSLAL
CCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHCCHHHHHHHHHHHCCCHHH
VEYQVARVATSLPYIPGLLSFREYPALLAAWAQLQQRPDLILVDGQGIAHPRRLGVASHF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHCHHHHC
GLLVDVPTIGVAKSRLCGDFLPLHQDVGAVQPLFDNDEQLGWVWRSKIRCNPLFISPGHR
CEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCC
VSVGSALAWVQRCMAGYRLPEPTRWADAIASNRPQFQRWLRKNPDFLGKRRDMI
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA