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The map label for this gene is greA

Identifier: 218930519

GI number: 218930519

Start: 3915367

End: 3915843

Strand: Reverse

Name: greA

Synonym: YPO3505

Alternate gene names: 218930519

Gene position: 3915843-3915367 (Counterclockwise)

Preceding gene: 218930523

Following gene: 218930517

Centisome position: 84.14

GC content: 42.35

Gene sequence:

>477_bases
ATGAAACAGATTCCGATGACGGTACGTGGCGCAGATAAGCTGCGTGAAGAGCTGGATTATTTAAAAGGTGTTCGTCGCCC
TAAAATTATTTCTGATATCGCTGATGCACGTGAACATGGCGACTTGAAAGAAAATGCGGAATATCACGCAGCCCGTGAGC
AACAAGGGTTCTGTGAAGGCCGAATTCAGGAAATAGAAGCCAAGCTTTCAAATGCTCAGGTAATTGATATCACTAAAATG
CCCAACAATGGCCGTGTTATTTTTGGTGCCACTGTTCGTGTATTAAATCTGAACACTGAAGAAGAACAGAACTACCGTAT
TGTGGGTGATGACGAGGCTGATTTTAAACAAAATCTTATTTCGGTTAATTCGCCTATTGCTCGTGGCCTGATTGGTAAAG
AAGTTGATGACGTCGTTGTTATCCATACGCCAGGCGGCGAAGTAGAATATGAAATTCTTAGCGTTGAATATGTGTAA

Upstream 100 bases:

>100_bases
TCGCTTGGAAGCGGATAGGGTATCAAGCTAACCGTATATTCACTCGAAAACGTTAGGATGACGGTTTTTTGAACAGGATA
GATTTAGAGGTATTTAAAAG

Downstream 100 bases:

>100_bases
TACTGGCCTGTATTGAACAGTAAATGCCCATTAAGTTATAAAATAAAGTTGTAAAGAAAAGTAAAAGGCCGCAAGCGGCC
TTTTATCAGAACAAAGTGCA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MKQIPMTVRGADKLREELDYLKGVRRPKIISDIADAREHGDLKENAEYHAAREQQGFCEGRIQEIEAKLSNAQVIDITKM
PNNGRVIFGATVRVLNLNTEEEQNYRIVGDDEADFKQNLISVNSPIARGLIGKEVDDVVVIHTPGGEVEYEILSVEYV

Sequences:

>Translated_158_residues
MKQIPMTVRGADKLREELDYLKGVRRPKIISDIADAREHGDLKENAEYHAAREQQGFCEGRIQEIEAKLSNAQVIDITKM
PNNGRVIFGATVRVLNLNTEEEQNYRIVGDDEADFKQNLISVNSPIARGLIGKEVDDVVVIHTPGGEVEYEILSVEYV
>Mature_158_residues
MKQIPMTVRGADKLREELDYLKGVRRPKIISDIADAREHGDLKENAEYHAAREQQGFCEGRIQEIEAKLSNAQVIDITKM
PNNGRVIFGATVRVLNLNTEEEQNYRIVGDDEADFKQNLISVNSPIARGLIGKEVDDVVVIHTPGGEVEYEILSVEYV

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=84.1772151898734, Blast_Score=279, Evalue=5e-77,
Organism=Escherichia coli, GI87082261, Length=158, Percent_Identity=34.8101265822785, Blast_Score=99, Evalue=1e-22,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_YERPE (Q8ZBB3)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AB0426
- RefSeq:   NP_668016.1
- RefSeq:   NP_991971.1
- RefSeq:   YP_002348394.1
- ProteinModelPortal:   Q8ZBB3
- SMR:   Q8ZBB3
- GeneID:   1145626
- GeneID:   1176324
- GeneID:   2764186
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO3505
- KEGG:   ypk:y0679
- KEGG:   ypm:YP_0578
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   YPES187410:Y0679-MONOMER
- BioCyc:   YPES214092:YPO3505-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17778; Mature: 17778

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQIPMTVRGADKLREELDYLKGVRRPKIISDIADAREHGDLKENAEYHAAREQQGFCEG
CCCCCCEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHH
RIQEIEAKLSNAQVIDITKMPNNGRVIFGATVRVLNLNTEEEQNYRIVGDDEADFKQNLI
HHHHHHHHHCCCEEEEEEECCCCCEEEEEEEEEEEECCCCCCCCEEEECCCCHHHHHHHH
SVNSPIARGLIGKEVDDVVVIHTPGGEVEYEILSVEYV
HCCCCHHHHHCCCCCCCEEEEECCCCCEEEEEEEEEEC
>Mature Secondary Structure
MKQIPMTVRGADKLREELDYLKGVRRPKIISDIADAREHGDLKENAEYHAAREQQGFCEG
CCCCCCEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHH
RIQEIEAKLSNAQVIDITKMPNNGRVIFGATVRVLNLNTEEEQNYRIVGDDEADFKQNLI
HHHHHHHHHCCCEEEEEEECCCCCEEEEEEEEEEEECCCCCCCCEEEECCCCHHHHHHHH
SVNSPIARGLIGKEVDDVVVIHTPGGEVEYEILSVEYV
HCCCCHHHHHCCCCCCCEEEEECCCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430