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The map label for this gene is truB [H]

Identifier: 218930508

GI number: 218930508

Start: 3902696

End: 3903670

Strand: Reverse

Name: truB [H]

Synonym: YPO3494

Alternate gene names: 218930508

Gene position: 3903670-3902696 (Counterclockwise)

Preceding gene: 218930509

Following gene: 218930507

Centisome position: 83.88

GC content: 49.44

Gene sequence:

>975_bases
ATGGGGCGTCCACGTCGTCGCGGCCGTGACATTAACGGCGTTTTGCTATTGGATAAGCCGCTAGGCCTCTCTTCTAATGA
CGTTTTGCAAAAAGTAAAACGTCTCTTTAGCGCTAATCGCGCTGGTCACACGGGTGCTCTTGATCCATTAGCAACCGGTA
TGTTGCCTATTTGTCTGGGCGAAGCAACTAAGTTTTCTCAATTCTTACTCGATTCAGATAAACGTTACCGAGTGGTTGCT
CGGCTGGGGCAACGCACGGATACCTCAGATGCGGAAGGTGCGTTAATCAGTGAGCGTGAGGTCAATCTTACTCAAGCTCA
GATCGATACTGCACTGGAGAGTTTCCGCGGTGAAAGCCAGCAGATCCCCTCCATGTATTCTGCATTGAAACATCAAGGTA
AGCCTCTTTACGAATATGCCCGTCAGGGGATTGAAGTCGAACGTGAAGCTCGCAGCATTACGGTTTACGAGTTGCTCTTC
ATCCGTTGGGAAGGCAATGATCTGGAACTGGAAATTCACTGTTCGAAAGGAACGTACATTCGTACCATCATCGATGACTT
GGGTGAATTATTGGGTTGCGGCGCACATGTCAGTTATCTGCGTCGCTTGCAGGTTGCGACTTACCCGAGCGAACGGATGG
TGACCCTAGAGCAGTTAACGGCGATGGTTGAGGCTGCGCAGGCAGAGGGACGCTCGCCAAATCCAGAACTGGATTCGCTG
TTATTGCCGATGGACAGTGCCGTTCTTAATTTTCCTGAAGTCAATCTGTTACCTTCCGTGGCCGCTTATGTAAAACAGGG
GCAACCGGTGCATGTGTCAGGTGCTCCCAGTGAAGGAATGGTCCGAATCACTGAAGGTAAAGAGCGTAATTTCATTGGTA
TTGGTACTATCGCAGAAGATGGCCGCGTTGCCCCTAAACGTTTGGTTGTGGAAAGCGTGGAAGTAGAAAATCTGCCAGTA
GAAAATAAAAAGTAG

Upstream 100 bases:

>100_bases
ACTCCCTGATTGAAGGGATGCGGATGTCTAACCTGGTGAGCAATGTCATCAAAAATGATGTAGAGCGTCAGGTTAATCCA
GGTAGTGACGAGGAGAAGTA

Downstream 100 bases:

>100_bases
GCCACACGGCCTGCCTTGCGATCCCGCGATTCAAAGAGTAGAATAGTGCGGCTTACATATTGGGTTGCTGAATTAGAGAT
CGGCGCCTGTCTTAATTCAT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MGRPRRRGRDINGVLLLDKPLGLSSNDVLQKVKRLFSANRAGHTGALDPLATGMLPICLGEATKFSQFLLDSDKRYRVVA
RLGQRTDTSDAEGALISEREVNLTQAQIDTALESFRGESQQIPSMYSALKHQGKPLYEYARQGIEVEREARSITVYELLF
IRWEGNDLELEIHCSKGTYIRTIIDDLGELLGCGAHVSYLRRLQVATYPSERMVTLEQLTAMVEAAQAEGRSPNPELDSL
LLPMDSAVLNFPEVNLLPSVAAYVKQGQPVHVSGAPSEGMVRITEGKERNFIGIGTIAEDGRVAPKRLVVESVEVENLPV
ENKK

Sequences:

>Translated_324_residues
MGRPRRRGRDINGVLLLDKPLGLSSNDVLQKVKRLFSANRAGHTGALDPLATGMLPICLGEATKFSQFLLDSDKRYRVVA
RLGQRTDTSDAEGALISEREVNLTQAQIDTALESFRGESQQIPSMYSALKHQGKPLYEYARQGIEVEREARSITVYELLF
IRWEGNDLELEIHCSKGTYIRTIIDDLGELLGCGAHVSYLRRLQVATYPSERMVTLEQLTAMVEAAQAEGRSPNPELDSL
LLPMDSAVLNFPEVNLLPSVAAYVKQGQPVHVSGAPSEGMVRITEGKERNFIGIGTIAEDGRVAPKRLVVESVEVENLPV
ENKK
>Mature_323_residues
GRPRRRGRDINGVLLLDKPLGLSSNDVLQKVKRLFSANRAGHTGALDPLATGMLPICLGEATKFSQFLLDSDKRYRVVAR
LGQRTDTSDAEGALISEREVNLTQAQIDTALESFRGESQQIPSMYSALKHQGKPLYEYARQGIEVEREARSITVYELLFI
RWEGNDLELEIHCSKGTYIRTIIDDLGELLGCGAHVSYLRRLQVATYPSERMVTLEQLTAMVEAAQAEGRSPNPELDSLL
LPMDSAVLNFPEVNLLPSVAAYVKQGQPVHVSGAPSEGMVRITEGKERNFIGIGTIAEDGRVAPKRLVVESVEVENLPVE
NKK

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=274, Percent_Identity=29.1970802919708, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI215599015, Length=209, Percent_Identity=30.622009569378, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI4503337, Length=209, Percent_Identity=30.622009569378, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI2367200, Length=311, Percent_Identity=72.0257234726688, Blast_Score=459, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17553978, Length=219, Percent_Identity=29.6803652968037, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323204, Length=278, Percent_Identity=28.0575539568345, Blast_Score=93, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6324037, Length=189, Percent_Identity=32.2751322751323, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281364189, Length=274, Percent_Identity=28.1021897810219, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281364187, Length=274, Percent_Identity=28.1021897810219, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281364185, Length=274, Percent_Identity=28.1021897810219, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281364183, Length=274, Percent_Identity=28.1021897810219, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI62471759, Length=274, Percent_Identity=28.1021897810219, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17975520, Length=274, Percent_Identity=28.1021897810219, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 35812; Mature: 35681

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRPRRRGRDINGVLLLDKPLGLSSNDVLQKVKRLFSANRAGHTGALDPLATGMLPICLG
CCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHC
EATKFSQFLLDSDKRYRVVARLGQRTDTSDAEGALISEREVNLTQAQIDTALESFRGESQ
CHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCHH
QIPSMYSALKHQGKPLYEYARQGIEVEREARSITVYELLFIRWEGNDLELEIHCSKGTYI
CCHHHHHHHHHCCCHHHHHHHCCCCEEHHHCEEEEEEEEEEEECCCEEEEEEEECCCCCH
RTIIDDLGELLGCGAHVSYLRRLQVATYPSERMVTLEQLTAMVEAAQAEGRSPNPELDSL
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHE
LLPMDSAVLNFPEVNLLPSVAAYVKQGQPVHVSGAPSEGMVRITEGKERNFIGIGTIAED
ECCCCHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCCCCEEEEEEECCC
GRVAPKRLVVESVEVENLPVENKK
CCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GRPRRRGRDINGVLLLDKPLGLSSNDVLQKVKRLFSANRAGHTGALDPLATGMLPICLG
CCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHC
EATKFSQFLLDSDKRYRVVARLGQRTDTSDAEGALISEREVNLTQAQIDTALESFRGESQ
CHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCHH
QIPSMYSALKHQGKPLYEYARQGIEVEREARSITVYELLFIRWEGNDLELEIHCSKGTYI
CCHHHHHHHHHCCCHHHHHHHCCCCEEHHHCEEEEEEEEEEEECCCEEEEEEEECCCCCH
RTIIDDLGELLGCGAHVSYLRRLQVATYPSERMVTLEQLTAMVEAAQAEGRSPNPELDSL
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHE
LLPMDSAVLNFPEVNLLPSVAAYVKQGQPVHVSGAPSEGMVRITEGKERNFIGIGTIAED
ECCCCHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCCCCEEEEEEECCC
GRVAPKRLVVESVEVENLPVENKK
CCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA