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The map label for this gene is fumA [H]

Identifier: 218930356

GI number: 218930356

Start: 3721374

End: 3723017

Strand: Direct

Name: fumA [H]

Synonym: YPO3335

Alternate gene names: 218930356

Gene position: 3721374-3723017 (Clockwise)

Preceding gene: 218930355

Following gene: 218930357

Centisome position: 79.97

GC content: 50.85

Gene sequence:

>1644_bases
ATGTCAAACAAACCGTTCCACTATCAGGATCCCTTCCCGCTCAAGGAAGATGATACTGAATATTACCTTGTCAGTGATAA
ACATGTCTCAGTTGCACAGTTTGAAGGACACGACATCCTAAAAGTCGAGCCGGAAGCCCTGACCCTCCTCGCTCAACAGG
CTTTCCACGATGCCTCTTTCCTCCTGCGCCCCGCTCACCAAAAACAAGTTGCCGCCATTCTGGATGACCCGGAAGCCAGC
GAAAACGATAAATATGTCGCCCTGCAATTCCTACGTAACTCTGAGATTTCGGCTAAAGGGATTTTACCCACCTGTCAAGA
TACCGGCACCGCGATCATCATGGGCAAAAAAGGCCAGCGGGTCTGGACTGGGGGTAACGATGCCGAAGCGTTATCACGTG
GGGTCTATAACACCTTTATTGAAGATAACCTGCGCTATTCACAAAACGCCGCGCTGGATATGTATAAGGAAGTGAATACC
GGTACCAACCTGCCAGCACAGATCGATCTATACAGCACCGAAGGTGAAGAGTACAAATTCTTGTTTGTGACTAAAGGCGG
CGGTTCAGCCAATAAAACCTATCTGTATCAGGAAACCAAAGCCCTGCTCACACCGGGCAGGCTGAAAGCGTTTTTGGTCG
AGAAAATGCGCACATTGGGTACCGCGGCTTGCCCGCCTTATCACATTGCCTTTGTCATCGGCGGTACCTCTGCCGAAAGT
ACGCTGAAGACGGTTAAACTGGCCTCGACCAAATATTACGATGGCTTGCCGACCGAAGGGAATGAACACGGCCAAGCGTT
CCGTGACACTGCGTTAGAGCAAGAATTGCTGGAAGCAGCACAAGATTTAGGTCTGGGCGCGCAATTTGGCGGCAAATACT
TTGCCCACGATGTTCGGGTCGTTCGCCTGCCACGCCACGGAGCCTCCTGCCCGGTCGGCATGGGGGTATCTTGTTCTGCT
GACCGTAATATCAAAGGCAAAATTAACCGCAAAGGCATTTGGTTGGAGAAACTGGAACAGAATCCAGGGAAATATATTCC
CGAACATTTGCGCCACAGCACTGAAGGCCAAGTGGTTAAAATTGATCTTAACCGCCCGATGCCTGAAATTTTAAAAGAGC
TGTCACAGTACCCGGTCTCCACCCGCCTGTCGTTAAGCGGCACCATTATTGTTGGCCGTGATATCGCCCATGCCAAATTA
AAAGAGCGGCTGGATAACGGTGAAGGCTTACCACAATACATTAAAGATCATCCGATCTATTACGCCGGACCGGCCAAAAC
CCCCGAAGGCTATGCTTCAGGATCACTCGGGCCAACCACCGCAGGGCGCATGGACTCTTACGTGGATCTGCTGCAATCCC
ACGGGGGTAGCATGATCATGTTAGCCAAAGGCAACCGTAGCCAGCAAGTGACCGACGCCTGCCATAAACACGGGGGCTTC
TATCTGGGCAGCATCGGTGGCCCGGCAGCGGTATTGGCGCAAAACAGTATTAAGAGTCTGGAATGTGTGGAATATCCTGA
ATTAGGGATGGAAGCCATCTGGAAGATTGAAGTGGAAGACTTCCCAGCCTTTATTTTAGTTGATGATAAGGGCAATGATT
TCTTCCAGCAGATCCAAGCGGCAAAATGCAACCGTTGTGGCTGA

Upstream 100 bases:

>100_bases
TATTTTTGATGATGATTAGATTTGATAATAATGACTTTTTACTGATGATTACTTCTGTTAACGAACATTTTCGCTAACGA
ATGTTGAATGTGAGAAAAAT

Downstream 100 bases:

>100_bases
GGTGAGTATTTAATACGCCATAAACTGGCCTCAACATATTGAGGTAATTTAGATTAAGCGGCTATCAAGGCCTGAGTTCT
TCATTCACGGAGCTCAGGCC

Product: fumarate hydratase, class I

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MSNKPFHYQDPFPLKEDDTEYYLVSDKHVSVAQFEGHDILKVEPEALTLLAQQAFHDASFLLRPAHQKQVAAILDDPEAS
ENDKYVALQFLRNSEISAKGILPTCQDTGTAIIMGKKGQRVWTGGNDAEALSRGVYNTFIEDNLRYSQNAALDMYKEVNT
GTNLPAQIDLYSTEGEEYKFLFVTKGGGSANKTYLYQETKALLTPGRLKAFLVEKMRTLGTAACPPYHIAFVIGGTSAES
TLKTVKLASTKYYDGLPTEGNEHGQAFRDTALEQELLEAAQDLGLGAQFGGKYFAHDVRVVRLPRHGASCPVGMGVSCSA
DRNIKGKINRKGIWLEKLEQNPGKYIPEHLRHSTEGQVVKIDLNRPMPEILKELSQYPVSTRLSLSGTIIVGRDIAHAKL
KERLDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGF
YLGSIGGPAAVLAQNSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQAAKCNRCG

Sequences:

>Translated_547_residues
MSNKPFHYQDPFPLKEDDTEYYLVSDKHVSVAQFEGHDILKVEPEALTLLAQQAFHDASFLLRPAHQKQVAAILDDPEAS
ENDKYVALQFLRNSEISAKGILPTCQDTGTAIIMGKKGQRVWTGGNDAEALSRGVYNTFIEDNLRYSQNAALDMYKEVNT
GTNLPAQIDLYSTEGEEYKFLFVTKGGGSANKTYLYQETKALLTPGRLKAFLVEKMRTLGTAACPPYHIAFVIGGTSAES
TLKTVKLASTKYYDGLPTEGNEHGQAFRDTALEQELLEAAQDLGLGAQFGGKYFAHDVRVVRLPRHGASCPVGMGVSCSA
DRNIKGKINRKGIWLEKLEQNPGKYIPEHLRHSTEGQVVKIDLNRPMPEILKELSQYPVSTRLSLSGTIIVGRDIAHAKL
KERLDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGF
YLGSIGGPAAVLAQNSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQAAKCNRCG
>Mature_546_residues
SNKPFHYQDPFPLKEDDTEYYLVSDKHVSVAQFEGHDILKVEPEALTLLAQQAFHDASFLLRPAHQKQVAAILDDPEASE
NDKYVALQFLRNSEISAKGILPTCQDTGTAIIMGKKGQRVWTGGNDAEALSRGVYNTFIEDNLRYSQNAALDMYKEVNTG
TNLPAQIDLYSTEGEEYKFLFVTKGGGSANKTYLYQETKALLTPGRLKAFLVEKMRTLGTAACPPYHIAFVIGGTSAEST
LKTVKLASTKYYDGLPTEGNEHGQAFRDTALEQELLEAAQDLGLGAQFGGKYFAHDVRVVRLPRHGASCPVGMGVSCSAD
RNIKGKINRKGIWLEKLEQNPGKYIPEHLRHSTEGQVVKIDLNRPMPEILKELSQYPVSTRLSLSGTIIVGRDIAHAKLK
ERLDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGFY
LGSIGGPAAVLAQNSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQAAKCNRCG

Specific function: It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=546, Percent_Identity=86.4468864468865, Blast_Score=1004, Evalue=0.0,
Organism=Escherichia coli, GI1790564, Length=546, Percent_Identity=85.8974358974359, Blast_Score=987, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=170, Percent_Identity=27.6470588235294, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 60132; Mature: 60001

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKPFHYQDPFPLKEDDTEYYLVSDKHVSVAQFEGHDILKVEPEALTLLAQQAFHDASF
CCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCEEEECHHHHHHHHHHHHCCHHH
LLRPAHQKQVAAILDDPEASENDKYVALQFLRNSEISAKGILPTCQDTGTAIIMGKKGQR
HCCCCCHHHHHHEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCE
VWTGGNDAEALSRGVYNTFIEDNLRYSQNAALDMYKEVNTGTNLPAQIDLYSTEGEEYKF
EECCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCEEE
LFVTKGGGSANKTYLYQETKALLTPGRLKAFLVEKMRTLGTAACPPYHIAFVIGGTSAES
EEEECCCCCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHH
TLKTVKLASTKYYDGLPTEGNEHGQAFRDTALEQELLEAAQDLGLGAQFGGKYFAHDVRV
HHHEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHCCCEEECCEEE
VRLPRHGASCPVGMGVSCSADRNIKGKINRKGIWLEKLEQNPGKYIPEHLRHSTEGQVVK
EEECCCCCCCCCCCCEECCCCCCCCCEECCCCCCHHHHHCCCCCCCHHHHCCCCCCCEEE
IDLNRPMPEILKELSQYPVSTRLSLSGTIIVGRDIAHAKLKERLDNGEGLPQYIKDHPIY
EECCCCHHHHHHHHHHCCCEEEEEECEEEEEECCHHHHHHHHHHCCCCCCHHHHHCCCEE
YAGPAKTPEGYASGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGF
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQNSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQA
EEECCCCCEEEHHHCCCCCCEECCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH
AKCNRCG
HHCCCCC
>Mature Secondary Structure 
SNKPFHYQDPFPLKEDDTEYYLVSDKHVSVAQFEGHDILKVEPEALTLLAQQAFHDASF
CCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCEEEECHHHHHHHHHHHHCCHHH
LLRPAHQKQVAAILDDPEASENDKYVALQFLRNSEISAKGILPTCQDTGTAIIMGKKGQR
HCCCCCHHHHHHEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCE
VWTGGNDAEALSRGVYNTFIEDNLRYSQNAALDMYKEVNTGTNLPAQIDLYSTEGEEYKF
EECCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCEEE
LFVTKGGGSANKTYLYQETKALLTPGRLKAFLVEKMRTLGTAACPPYHIAFVIGGTSAES
EEEECCCCCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHH
TLKTVKLASTKYYDGLPTEGNEHGQAFRDTALEQELLEAAQDLGLGAQFGGKYFAHDVRV
HHHEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHCCCEEECCEEE
VRLPRHGASCPVGMGVSCSADRNIKGKINRKGIWLEKLEQNPGKYIPEHLRHSTEGQVVK
EEECCCCCCCCCCCCEECCCCCCCCCEECCCCCCHHHHHCCCCCCCHHHHCCCCCCCEEE
IDLNRPMPEILKELSQYPVSTRLSLSGTIIVGRDIAHAKLKERLDNGEGLPQYIKDHPIY
EECCCCHHHHHHHHHHCCCEEEEEECEEEEEECCHHHHHHHHHHCCCCCCHHHHHCCCEE
YAGPAKTPEGYASGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGF
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQNSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQA
EEECCCCCEEEHHHCCCCCCEECCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH
AKCNRCG
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 1879695 [H]