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The map label for this gene is gatA [H]

Identifier: 218930286

GI number: 218930286

Start: 3632668

End: 3634065

Strand: Direct

Name: gatA [H]

Synonym: YPO3261

Alternate gene names: 218930286

Gene position: 3632668-3634065 (Clockwise)

Preceding gene: 218930285

Following gene: 218930287

Centisome position: 78.06

GC content: 55.01

Gene sequence:

>1398_bases
ATGAAGCCTGCCTCATCACTCACTATCAGCGAGATCCAACAAGCCCTGCACAAGGGCGAATTATCCGCACAAGCGATCGC
ACAGCAGACGTTGGACGCCATTACCGTCGCCAATCCGCTCATCAACGCTTATACCCATATCACCGCTGACCGTATGCTTA
GCGAAGCTTGTCGGCTAGATACGCTACGCGCCAAAGGCCAGCCGCTGCCAGCACTGGCGGCAGTCCCTTATGCGGTCAAG
AATCTGTTCGATGTTGCCGGAGAAACAACCCTATCAGGTGCCAGCTTATTTTGTGATCAAGCCCCCGCCAAACAGGATGC
CTGGGCCATTTCATGCCTGAGCCGCCAAGGAGCCATGCTCTCCGGCATGTTAAATATGGATGCCTATGCTTACGGTTTTA
CTACCGAGAACAGCCATTATGGTGCCACCCATAATCCACACGATCTGAACCGCATTGCAGGGGGATCTTCTGGCGGATCT
GCGGCAGCGGTCGCCGCAGGGCTCGTGCACTTTTCTCTCGGCAGTGATACTAACGGTTCGATCCGGGTTCCCTCCTCACT
GTGTGGTATTTTTGGGCTGAAACCAACCTTTGGCCGCCTGTCACGCCGCGGCACCCACCCATTTGTCGCCAGTCTGGACC
ATATCGGCCCAATGGCACGCTGTACGCAAGATTTGGCGGTGGTTTATGACGTGATGCAAGGCATCGATACTCAGGATAAT
TTTCAAGCCGATCAACCCGTCACGCCGTGCCAAAATTTACTGACGCGTGGTCAGCAAGGGTTAAGAAGCGCGGTGCTGGG
CGGTTACTTTCATCAATGGAGTGATGATGACGCCAAAGCCGCAGTGCTGGCGGTCGCAAAGGCGCTTGAGGCTAACGCGG
AGGTCGAGATGCCGCAGAGTGATTTAGCCCGGTCAGCGGCATTTATTATCAGTGCCAGCGAGGGCGGCAATCAATATCTG
CCCGCCTTGAGGAAATCACCTCAACGTTTTGAACCCCTATCACGGGAGCGGTTATTAGCCGGAGCCATGCTACCGGCAAC
TTGGTACGTACAGGCCCAGCGTTTCCGTCATCATTTTCGCCAACACATTTTGCCGCTGTTCGAGCACTGGGATATTTTGA
TTGCCCCAGCAACGCCATGCAGTGCCACGCTTATCGGCCAGGACACGATGAGGATTAATGATATGGACCTACCGATCCGC
GCCAGTATGGGCATATTAACCCAGCCAATCTCCTTCCTGGGTCTGCCAGTCGTGAGCGTACCCTTGAAAACCACCCGCGG
CTTACCAATTGGCATTCAGTTGATTGCCCCACCATGGCGAGAAGATTTATGCCTGCGCGCGGCTTATGCGCTGGAGCAAC
AAGGGTTGGTCAGCGCGCCGGTGAGTACTGTTATTTGA

Upstream 100 bases:

>100_bases
CGGCATTGCAGCAACAATTTGAACACATTGCCGCTCTGGCCGCACCGCTGATGGCCCTACCTTTAGATGAACGGCAAGAG
ATCGCGGGGGTATACCGGTT

Downstream 100 bases:

>100_bases
TAGGACCGTTATTTGATAGGACCGCTATTTGATAGGTCCGTTATTTGATAGGACCGTGCTTGATAAAGATAGCCATTTGG
CGAGAACCGTCTCCGATGAG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MKPASSLTISEIQQALHKGELSAQAIAQQTLDAITVANPLINAYTHITADRMLSEACRLDTLRAKGQPLPALAAVPYAVK
NLFDVAGETTLSGASLFCDQAPAKQDAWAISCLSRQGAMLSGMLNMDAYAYGFTTENSHYGATHNPHDLNRIAGGSSGGS
AAAVAAGLVHFSLGSDTNGSIRVPSSLCGIFGLKPTFGRLSRRGTHPFVASLDHIGPMARCTQDLAVVYDVMQGIDTQDN
FQADQPVTPCQNLLTRGQQGLRSAVLGGYFHQWSDDDAKAAVLAVAKALEANAEVEMPQSDLARSAAFIISASEGGNQYL
PALRKSPQRFEPLSRERLLAGAMLPATWYVQAQRFRHHFRQHILPLFEHWDILIAPATPCSATLIGQDTMRINDMDLPIR
ASMGILTQPISFLGLPVVSVPLKTTRGLPIGIQLIAPPWREDLCLRAAYALEQQGLVSAPVSTVI

Sequences:

>Translated_465_residues
MKPASSLTISEIQQALHKGELSAQAIAQQTLDAITVANPLINAYTHITADRMLSEACRLDTLRAKGQPLPALAAVPYAVK
NLFDVAGETTLSGASLFCDQAPAKQDAWAISCLSRQGAMLSGMLNMDAYAYGFTTENSHYGATHNPHDLNRIAGGSSGGS
AAAVAAGLVHFSLGSDTNGSIRVPSSLCGIFGLKPTFGRLSRRGTHPFVASLDHIGPMARCTQDLAVVYDVMQGIDTQDN
FQADQPVTPCQNLLTRGQQGLRSAVLGGYFHQWSDDDAKAAVLAVAKALEANAEVEMPQSDLARSAAFIISASEGGNQYL
PALRKSPQRFEPLSRERLLAGAMLPATWYVQAQRFRHHFRQHILPLFEHWDILIAPATPCSATLIGQDTMRINDMDLPIR
ASMGILTQPISFLGLPVVSVPLKTTRGLPIGIQLIAPPWREDLCLRAAYALEQQGLVSAPVSTVI
>Mature_465_residues
MKPASSLTISEIQQALHKGELSAQAIAQQTLDAITVANPLINAYTHITADRMLSEACRLDTLRAKGQPLPALAAVPYAVK
NLFDVAGETTLSGASLFCDQAPAKQDAWAISCLSRQGAMLSGMLNMDAYAYGFTTENSHYGATHNPHDLNRIAGGSSGGS
AAAVAAGLVHFSLGSDTNGSIRVPSSLCGIFGLKPTFGRLSRRGTHPFVASLDHIGPMARCTQDLAVVYDVMQGIDTQDN
FQADQPVTPCQNLLTRGQQGLRSAVLGGYFHQWSDDDAKAAVLAVAKALEANAEVEMPQSDLARSAAFIISASEGGNQYL
PALRKSPQRFEPLSRERLLAGAMLPATWYVQAQRFRHHFRQHILPLFEHWDILIAPATPCSATLIGQDTMRINDMDLPIR
ASMGILTQPISFLGLPVVSVPLKTTRGLPIGIQLIAPPWREDLCLRAAYALEQQGLVSAPVSTVI

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=514, Percent_Identity=28.2101167315175, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI195972892, Length=244, Percent_Identity=31.1475409836066, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI166795287, Length=503, Percent_Identity=26.2425447316103, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=444, Percent_Identity=26.3513513513513, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17556264, Length=294, Percent_Identity=29.2517006802721, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17537465, Length=499, Percent_Identity=25.8517034068136, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=258, Percent_Identity=26.7441860465116, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=464, Percent_Identity=23.7068965517241, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=170, Percent_Identity=34.7058823529412, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=170, Percent_Identity=34.7058823529412, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=449, Percent_Identity=26.7260579064588, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323950, Length=248, Percent_Identity=29.0322580645161, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320448, Length=230, Percent_Identity=32.1739130434783, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24648113, Length=494, Percent_Identity=31.5789473684211, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24644968, Length=499, Percent_Identity=26.0521042084168, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24652985, Length=485, Percent_Identity=28.4536082474227, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI19922090, Length=485, Percent_Identity=28.4536082474227, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24652981, Length=485, Percent_Identity=28.4536082474227, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24652983, Length=485, Percent_Identity=28.4536082474227, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI161078093, Length=496, Percent_Identity=27.4193548387097, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI45550774, Length=495, Percent_Identity=24.2424242424242, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24648435, Length=495, Percent_Identity=24.2424242424242, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24648437, Length=495, Percent_Identity=24.2424242424242, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24648441, Length=432, Percent_Identity=25, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648439, Length=432, Percent_Identity=25, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356731, Length=183, Percent_Identity=34.9726775956284, Blast_Score=92, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 49873; Mature: 49873

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPASSLTISEIQQALHKGELSAQAIAQQTLDAITVANPLINAYTHITADRMLSEACRLD
CCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLRAKGQPLPALAAVPYAVKNLFDVAGETTLSGASLFCDQAPAKQDAWAISCLSRQGAML
HHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCHHH
SGMLNMDAYAYGFTTENSHYGATHNPHDLNRIAGGSSGGSAAAVAAGLVHFSLGSDTNGS
HHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHEECCCCCCCC
IRVPSSLCGIFGLKPTFGRLSRRGTHPFVASLDHIGPMARCTQDLAVVYDVMQGIDTQDN
EECCHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCC
FQADQPVTPCQNLLTRGQQGLRSAVLGGYFHQWSDDDAKAAVLAVAKALEANAEVEMPQS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHH
DLARSAAFIISASEGGNQYLPALRKSPQRFEPLSRERLLAGAMLPATWYVQAQRFRHHFR
HHHCCEEEEEEECCCCCCHHHHHHCCCHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHH
QHILPLFEHWDILIAPATPCSATLIGQDTMRINDMDLPIRASMGILTQPISFLGLPVVSV
HHHHHHHHCCCEEEECCCCCCEEEECCCCEEECCCCCCHHHCCCHHHCCHHHHCCCCEEE
PLKTTRGLPIGIQLIAPPWREDLCLRAAYALEQQGLVSAPVSTVI
CCHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHCC
>Mature Secondary Structure
MKPASSLTISEIQQALHKGELSAQAIAQQTLDAITVANPLINAYTHITADRMLSEACRLD
CCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLRAKGQPLPALAAVPYAVKNLFDVAGETTLSGASLFCDQAPAKQDAWAISCLSRQGAML
HHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCHHH
SGMLNMDAYAYGFTTENSHYGATHNPHDLNRIAGGSSGGSAAAVAAGLVHFSLGSDTNGS
HHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHEECCCCCCCC
IRVPSSLCGIFGLKPTFGRLSRRGTHPFVASLDHIGPMARCTQDLAVVYDVMQGIDTQDN
EECCHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCC
FQADQPVTPCQNLLTRGQQGLRSAVLGGYFHQWSDDDAKAAVLAVAKALEANAEVEMPQS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHH
DLARSAAFIISASEGGNQYLPALRKSPQRFEPLSRERLLAGAMLPATWYVQAQRFRHHFR
HHHCCEEEEEEECCCCCCHHHHHHCCCHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHH
QHILPLFEHWDILIAPATPCSATLIGQDTMRINDMDLPIRASMGILTQPISFLGLPVVSV
HHHHHHHHCCCEEEECCCCCCEEEECCCCEEECCCCCCHHHCCCHHHCCHHHHCCCCEEE
PLKTTRGLPIGIQLIAPPWREDLCLRAAYALEQQGLVSAPVSTVI
CCHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA