Click here to switch to the map view.

The map label for this gene is gcvR [H]

Identifier: 218930108

GI number: 218930108

Start: 3420361

End: 3420930

Strand: Direct

Name: gcvR [H]

Synonym: YPO3063

Alternate gene names: 218930108

Gene position: 3420361-3420930 (Clockwise)

Preceding gene: 218930100

Following gene: 218930109

Centisome position: 73.5

GC content: 48.77

Gene sequence:

>570_bases
TTGCCGCAGCTTGATGAACATTATCTGGTCATTACCGCACTCGGTGCTGACCGCCCAGGGATCGTCAATACCATCACCCG
CCATGTCAGTAGCTGCGGTTGTAATATTGAAGATAGTCGGCTGGCTATGTTCGGTGAGGAGTTCACCTTTATTATGCTGC
TGTCAGGCAGTTGGAACGCCGTTACCCTGCTGGAATCAACTCTGCCGCAAAAAGGTGCAGAGCTTGATTTGCTGATTGTC
ATGAAGCGAACCAACGCTCAAGGGCAGAAAGCTATGCCAACCACTGTCTGGGTAAAAGTTGAAGTGAATGACTCCCCCCA
CATTATTGAACGGTTTACCAACCTGTTCCATTGCCACAATATGAATATCGCGGAGTTAGTCTCCAAAACTCAGCCGGCAG
ACGGCGAGACACCGCCATTGCTACATATACAGATGAGTGCACACAGTCCTGCCAGCCAAAACAGTTCAATAATCGAACAT
GATTTTTATCAACTCTGTACAGAACTCAACGCGCAGGGCAGTATTAGCGTGGTGAGCTATCCACAGCATGATGACCGAAT
GGAGAGTTAG

Upstream 100 bases:

>100_bases
TATCCAAAAGCAAGTAAACAACTGTGTTGTAAGCGCTTGGCAGCAGGTTTTTTTTATGTTTACCATGGTAACCCCAAAGA
GCATGACAGGAAGAGCGATT

Downstream 100 bases:

>100_bases
TGATGAGCCCATTGAAAGCCGGTGATATAGCGCCGAAATTTAGTTTACCCGATCAAGATGGCGAGCAAATTAGTTTGGCC
GACTTCCAGGGACAGCGTGT

Product: glycine cleavage system transcriptional repressor

Products: NA

Alternate protein names: Gcv operon repressor [H]

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MPQLDEHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAMFGEEFTFIMLLSGSWNAVTLLESTLPQKGAELDLLIV
MKRTNAQGQKAMPTTVWVKVEVNDSPHIIERFTNLFHCHNMNIAELVSKTQPADGETPPLLHIQMSAHSPASQNSSIIEH
DFYQLCTELNAQGSISVVSYPQHDDRMES

Sequences:

>Translated_189_residues
MPQLDEHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAMFGEEFTFIMLLSGSWNAVTLLESTLPQKGAELDLLIV
MKRTNAQGQKAMPTTVWVKVEVNDSPHIIERFTNLFHCHNMNIAELVSKTQPADGETPPLLHIQMSAHSPASQNSSIIEH
DFYQLCTELNAQGSISVVSYPQHDDRMES
>Mature_188_residues
PQLDEHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAMFGEEFTFIMLLSGSWNAVTLLESTLPQKGAELDLLIVM
KRTNAQGQKAMPTTVWVKVEVNDSPHIIERFTNLFHCHNMNIAELVSKTQPADGETPPLLHIQMSAHSPASQNSSIIEHD
FYQLCTELNAQGSISVVSYPQHDDRMES

Specific function: Negative transcriptional regulator of the glycine cleavage system operon (GCV). Does not autoregulate its own expression. It is not yet known how gcvR acts as a repressor. It does not seem to bind DNA. It could interact with gcvA and suppress its activato

COG id: COG2716

COG function: function code E; Glycine cleavage system regulatory protein

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082113, Length=181, Percent_Identity=75.6906077348066, Blast_Score=290, Evalue=6e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR016867 [H]

Pfam domain/function: PF01842 ACT [H]

EC number: NA

Molecular weight: Translated: 21004; Mature: 20873

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQLDEHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAMFGEEFTFIMLLSGSWNA
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEECCCCE
VTLLESTLPQKGAELDLLIVMKRTNAQGQKAMPTTVWVKVEVNDSPHIIERFTNLFHCHN
EEEEHHHCCCCCCCEEEEEEEECCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCC
MNIAELVSKTQPADGETPPLLHIQMSAHSPASQNSSIIEHDFYQLCTELNAQGSISVVSY
CCHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEC
PQHDDRMES
CCCCCCCCC
>Mature Secondary Structure 
PQLDEHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAMFGEEFTFIMLLSGSWNA
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEECCCCE
VTLLESTLPQKGAELDLLIVMKRTNAQGQKAMPTTVWVKVEVNDSPHIIERFTNLFHCHN
EEEEHHHCCCCCCCEEEEEEEECCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCC
MNIAELVSKTQPADGETPPLLHIQMSAHSPASQNSSIIEHDFYQLCTELNAQGSISVVSY
CCHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEC
PQHDDRMES
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537378; 9205837; 9278503; 2016588 [H]