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The map label for this gene is dapE [H]

Identifier: 218930098

GI number: 218930098

Start: 3410745

End: 3411872

Strand: Direct

Name: dapE [H]

Synonym: YPO3053

Alternate gene names: 218930098

Gene position: 3410745-3411872 (Clockwise)

Preceding gene: 218930097

Following gene: 218930099

Centisome position: 73.29

GC content: 52.3

Gene sequence:

>1128_bases
ATGATCTGTCCGGTAATCGAATTGGCCCAACAGTTAATTAAACGCCCATCGCTCAGCCCTTCAGATGCGGGTTGCCAAGA
GATCATGATCCAGCGCCTAGCCGCCATCGGCTTTACCATCGAACCCATGAATTTTGGTGATACGCTTAATTTTTGGGCAT
GGCGTGGCGAAGGCGAAACGCTGGCATTTGCTGGTCACACGGATGTGGTGCCTACCGGTGACGAGAGCCACTGGCACAGC
CCACCGTTTGAACCCACCATCCGTGACGGCATGTTGTATGGCCGAGGTGCCGCAGACATGAAAGGCTCACTGGCAGCAAT
GATCGTAGCAGCCGAGCGTTTTGTCGCCGCGCACCCTGACCACAAAGGCCGCCTGGCCTTTATGATAACCTCCGATGAAG
AAGCCAAAGCCACCAACGGTACAGTAAAAGTGGTTGAAGCCCTGATGGCACGCCACGAGCGGCTGGATTATTGTCTGGTG
GGCGAACCATCCAGTACTGACCGGGTTGGCGATATCGTGAAAAATGGCCGCCGTGGCTCCATCACCGCTAACTTGCGTAT
TCATGGAGTGCAAGGGCACGTGGCGTATCCTCATCTGGCGGATAATCCCGTTCATCGTGCTATGCCAGCGCTGAATGAAT
TGGTTGCCACCCAATGGGATGAAGGCAATGCGTTTTTCCCTGCCACCAGTATGCAAATTGCCAATTTACAGGCCGGTACG
GGCAGCAATAATGTTATTCCCGGTGAATTTTATGTGCAGTTTAACTTCCGTTTCAGTACTGAACTGACTGACAGCCTGAT
CAAGCAACGGGTAGCCGCATTGCTGGATCGACATCAGCTTGATTACACCCTGGAATGGGTATTGTCTGGTCAACCTTTCC
TGACCGCCAAAGGGGCGTTGGTGGATGCCGTGGTTAATGCCGTTAAGCATTACACTGAAATCACACCGCAACTGTTAACC
ACCGGGGGGACGTCAGATGGCCGCTTTATTGCGTTAATGGGGGCGCAAGTGGTTGAGTTGGGGCCAGTTAATGCCACCAT
TCATAAAGTTAATGAATGTGTTAGCGCGGCTGATTTACAATTGCTCAGCCGTATGTACCAGAAAATCATGGAACAACTGA
TCGCATGA

Upstream 100 bases:

>100_bases
CCACTGCTGGAAGGCCCGAATGGTGAGATGCTGCTAGGCTTCAAAATTGAAAGCTATCAAGAATTTATTCATAACCAACC
CGCTACTGAGGTGCAATAAC

Downstream 100 bases:

>100_bases
CCGATAATACATTAACGCCGCAGATGCTTACTGGCCTTTCGACAGATCATTTGGTGGTGTTAAGTGGCGCTCATCGCCTA
CAGCCACAGGCGGCTGAGGC

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MICPVIELAQQLIKRPSLSPSDAGCQEIMIQRLAAIGFTIEPMNFGDTLNFWAWRGEGETLAFAGHTDVVPTGDESHWHS
PPFEPTIRDGMLYGRGAADMKGSLAAMIVAAERFVAAHPDHKGRLAFMITSDEEAKATNGTVKVVEALMARHERLDYCLV
GEPSSTDRVGDIVKNGRRGSITANLRIHGVQGHVAYPHLADNPVHRAMPALNELVATQWDEGNAFFPATSMQIANLQAGT
GSNNVIPGEFYVQFNFRFSTELTDSLIKQRVAALLDRHQLDYTLEWVLSGQPFLTAKGALVDAVVNAVKHYTEITPQLLT
TGGTSDGRFIALMGAQVVELGPVNATIHKVNECVSAADLQLLSRMYQKIMEQLIA

Sequences:

>Translated_375_residues
MICPVIELAQQLIKRPSLSPSDAGCQEIMIQRLAAIGFTIEPMNFGDTLNFWAWRGEGETLAFAGHTDVVPTGDESHWHS
PPFEPTIRDGMLYGRGAADMKGSLAAMIVAAERFVAAHPDHKGRLAFMITSDEEAKATNGTVKVVEALMARHERLDYCLV
GEPSSTDRVGDIVKNGRRGSITANLRIHGVQGHVAYPHLADNPVHRAMPALNELVATQWDEGNAFFPATSMQIANLQAGT
GSNNVIPGEFYVQFNFRFSTELTDSLIKQRVAALLDRHQLDYTLEWVLSGQPFLTAKGALVDAVVNAVKHYTEITPQLLT
TGGTSDGRFIALMGAQVVELGPVNATIHKVNECVSAADLQLLSRMYQKIMEQLIA
>Mature_375_residues
MICPVIELAQQLIKRPSLSPSDAGCQEIMIQRLAAIGFTIEPMNFGDTLNFWAWRGEGETLAFAGHTDVVPTGDESHWHS
PPFEPTIRDGMLYGRGAADMKGSLAAMIVAAERFVAAHPDHKGRLAFMITSDEEAKATNGTVKVVEALMARHERLDYCLV
GEPSSTDRVGDIVKNGRRGSITANLRIHGVQGHVAYPHLADNPVHRAMPALNELVATQWDEGNAFFPATSMQIANLQAGT
GSNNVIPGEFYVQFNFRFSTELTDSLIKQRVAALLDRHQLDYTLEWVLSGQPFLTAKGALVDAVVNAVKHYTEITPQLLT
TGGTSDGRFIALMGAQVVELGPVNATIHKVNECVSAADLQLLSRMYQKIMEQLIA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=375, Percent_Identity=81.8666666666667, Blast_Score=654, Evalue=0.0,
Organism=Escherichia coli, GI1790395, Length=259, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 40933; Mature: 40933

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MICPVIELAQQLIKRPSLSPSDAGCQEIMIQRLAAIGFTIEPMNFGDTLNFWAWRGEGET
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCEEEEECCCCE
LAFAGHTDVVPTGDESHWHSPPFEPTIRDGMLYGRGAADMKGSLAAMIVAAERFVAAHPD
EEEECCCCCCCCCCCCCCCCCCCCCCHHCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCC
HKGRLAFMITSDEEAKATNGTVKVVEALMARHERLDYCLVGEPSSTDRVGDIVKNGRRGS
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCCE
ITANLRIHGVQGHVAYPHLADNPVHRAMPALNELVATQWDEGNAFFPATSMQIANLQAGT
EEEEEEEEEEECCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCEEEEEEECCC
GSNNVIPGEFYVQFNFRFSTELTDSLIKQRVAALLDRHQLDYTLEWVLSGQPFLTAKGAL
CCCCCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEECHHH
VDAVVNAVKHYTEITPQLLTTGGTSDGRFIALMGAQVVELGPVNATIHKVNECVSAADLQ
HHHHHHHHHHHHHCCHHHEECCCCCCCCEEEEECCEEEEECCCCHHHHHHHHHHHHHHHH
LLSRMYQKIMEQLIA
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MICPVIELAQQLIKRPSLSPSDAGCQEIMIQRLAAIGFTIEPMNFGDTLNFWAWRGEGET
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCEEEEECCCCE
LAFAGHTDVVPTGDESHWHSPPFEPTIRDGMLYGRGAADMKGSLAAMIVAAERFVAAHPD
EEEECCCCCCCCCCCCCCCCCCCCCCHHCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCC
HKGRLAFMITSDEEAKATNGTVKVVEALMARHERLDYCLVGEPSSTDRVGDIVKNGRRGS
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCCE
ITANLRIHGVQGHVAYPHLADNPVHRAMPALNELVATQWDEGNAFFPATSMQIANLQAGT
EEEEEEEEEEECCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCEEEEEEECCC
GSNNVIPGEFYVQFNFRFSTELTDSLIKQRVAALLDRHQLDYTLEWVLSGQPFLTAKGAL
CCCCCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEECHHH
VDAVVNAVKHYTEITPQLLTTGGTSDGRFIALMGAQVVELGPVNATIHKVNECVSAADLQ
HHHHHHHHHHHHHCCHHHEECCCCCCCCEEEEECCEEEEECCCCHHHHHHHHHHHHHHHH
LLSRMYQKIMEQLIA
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA